Genotoxic Hazard and Oxidative Stress Induced by Wastewater Irrigated Soil With Special Reference to Pesticides and Heavy Metal Pollution

Mapping Intimacies ◽

10.21203/rs.3.rs-488039/v1 ◽

2021 ◽

Author(s):

Mohammad Tarique Zeyad ◽

Abdul Malik

Keyword(s):

Oxidative Stress ◽

Dna Repair ◽

Contaminated Soil ◽

Soil Extract ◽

Mutagenic Potential ◽

Potential Health ◽

Soil Extracts ◽

Plasmid Nicking Assay ◽

Plasmid Nicking ◽

K 12

Abstract Enhancement of industrial growth and urbanization, soil contamination is increasingly prominent. Therefore, it’s important to examine possible adverse effects of industrial waste. Samples were found to be polluted with several heavy-metals and pesticides. Gas chromatographic results showed occurrence of high-level of organochlorine and organophosphate pesticides. Genotoxicity of soil extracts was assessed using environmental-risk assessment models. Soil samples were extracted in hexane and dichloromethane solvents and were evaluated for genotoxic potential by prokaryotic (Ames test, plasmid nicking assay and E. coli K-12 DNA repair defective mutants) and eukaryotic (Allium cepa root chromosomal aberration and Vigna radiata seed-germination test) bioassays. Strain TA98 was found most susceptible among soil extracts. Hexane soil extract of wastewater irrigation was determined more mutagenic than DCM samples in terms of mutagenic index, mutagenic potential and induction factor for Ames strains. The damage in DNA repair defective mutants of hexane extracts were found higher compared to DCM extracts at dose of 20 μl/ml of culture. Survival in polA, lexA and recA mutants were 39%, 47% and 55% while treated with hexane extract. Allium cepa test, mitotic index was decreased in dose-dependent way and various kinds of chromosomal aberrations were found. Vigna radiata seeds germination and other parameters were also affected when treated with contaminated soil. Oxidative stress in V. radiata roots were also showed under CLS microscope. Genotoxicity of contaminated soil extract was also confirmed by plasmid nicking test. Our study provides possible explanation for the assessment of potential health and environmental hazards of the industrial region.

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SERS approach for Zn(II) detection in contaminated soil

Open Chemistry ◽

10.2478/s11532-011-0019-5 ◽

2011 ◽

Vol 9 (3) ◽

pp. 410-414 ◽

Cited By ~ 5

Author(s):

László Szabó ◽

Loredana Leopold ◽

Bogdan Cozar ◽

Nicolae Leopold ◽

Krisztian Herman ◽

...

Keyword(s):

Contaminated Soil ◽

Metal Ion ◽

Density Functional ◽

Soil Extract ◽

Sers Spectrum ◽

Industrialized Countries ◽

Soil Extracts ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Enhanced Raman Scattering

AbstractSoil contamination by metals is a common problem encountered in many industrialized countries. In this work we present a new approach for heavy metals detection by using surface-enhanced Raman scattering (SERS) spectroscopy. Zn(II) can be clearly determined by SERS in contaminated soil by using 4-(2-pyridylazo) resorcinol (PAR) as chelating molecule for the metal ion. The SERS spectra of PAR, of its metal chelates and of the soil extract-PAR mixture were recorded using a hydroxylamine reduced silver colloid. An excellent match of the PAR-contaminated soil extracts SERS spectrum to the Zn(PAR)2 SERS spectrum can be observed, demonstrating the presence of Zn(II) in the soil probes. Density functional theory (DFT) based calculations were also performed for a reliable assignment of SERS spectra.

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Genetic variants and cognitive functions in patients with brain tumors

Neuro-Oncology ◽

10.1093/neuonc/noz094 ◽

2019 ◽

Vol 21 (10) ◽

pp. 1297-1309 ◽

Cited By ~ 5

Author(s):

Denise D Correa ◽

Jaya Satagopan ◽

Axel Martin ◽

Erica Braun ◽

Maria Kryza-Lacombe ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Cycle ◽

Dna Repair ◽

Brain Tumor ◽

Brain Tumors ◽

Cell Cycle Regulation ◽

Cognitive Outcome ◽

Cholesterol Transport ◽

Tumor Patients ◽

Cycle Regulation

AbstractBackgroundPatients with brain tumors treated with radiotherapy (RT) and chemotherapy (CT) often experience cognitive dysfunction. We reported that single nucleotide polymorphisms (SNPs) in the APOE, COMT, and BDNF genes may influence cognition in brain tumor patients. In this study, we assessed whether genes associated with late-onset Alzheimer’s disease (LOAD), inflammation, cholesterol transport, dopamine and myelin regulation, and DNA repair may influence cognitive outcome in this population.MethodsOne hundred and fifty brain tumor patients treated with RT ± CT or CT alone completed a neurocognitive assessment and provided a blood sample for genotyping. We genotyped genes/SNPs in these pathways: (i) LOAD risk/inflammation/cholesterol transport, (ii) dopamine regulation, (iii) myelin regulation, (iv) DNA repair, (v) blood–brain barrier disruption, (vi) cell cycle regulation, and (vii) response to oxidative stress. White matter (WM) abnormalities were rated on brain MRIs.ResultsMultivariable linear regression analysis with Bayesian shrinkage estimation of SNP effects, adjusting for relevant demographic, disease, and treatment variables, indicated strong associations (posterior association summary [PAS] ≥ 0.95) among tests of attention, executive functions, and memory and 33 SNPs in genes involved in: LOAD/inflammation/cholesterol transport (eg, PDE7A, IL-6), dopamine regulation (eg, DRD1, COMT), myelin repair (eg, TCF4), DNA repair (eg, RAD51), cell cycle regulation (eg, SESN1), and response to oxidative stress (eg, GSTP1). The SNPs were not significantly associated with WM abnormalities.ConclusionThis novel study suggests that polymorphisms in genes involved in aging and inflammation, dopamine, myelin and cell cycle regulation, and DNA repair and response to oxidative stress may be associated with cognitive outcome in patients with brain tumors.

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Caveolin-1 promotes radioresistance in rhabdomyosarcoma through increased oxidative stress protection and DNA repair

Cancer Letters ◽

10.1016/j.canlet.2021.02.005 ◽

2021 ◽

Vol 505 ◽

pp. 1-12

Author(s):

Silvia Codenotti ◽

Francesco Marampon ◽

Luca Triggiani ◽

Marco Lorenzo Bonù ◽

Stefano Maria Magrini ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Repair ◽

Caveolin 1 ◽

Stress Protection ◽

Oxidative Stress Protection

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Potential Antioxidant and Anti-Inflammatory Effects of Spilanthes acmella and Its Health Beneficial Effects: A Review

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18073532 ◽

2021 ◽

Vol 18 (7) ◽

pp. 3532

Author(s):

Rohanizah Abdul Rahim ◽

Putri Ayu Jayusman ◽

Norliza Muhammad ◽

Norazlina Mohamed ◽

Vuanghao Lim ◽

...

Keyword(s):

Oxidative Stress ◽

Potential Role ◽

Radical Scavenging ◽

Mapk Signaling ◽

Total Phenolic ◽

Thiobarbituric Acid Reactive Substance ◽

Potential Health ◽

Beneficial Effects ◽

Spilanthes Acmella ◽

Anti Inflammatory

Oxidative stress and inflammation are two common risk factors of various life-threatening disease pathogenesis. In recent years, medicinal plants that possess antioxidant and anti-inflammatory activities were extensively studied for their potential role in treating and preventing diseases. Spilanthes acmella (S. acmella), which has been traditionally used to treat toothache in Malaysia, contains various active metabolites responsible for its anti-inflammatory, antiseptic, and anesthetic bioactivities. These bioactivities were attributed to bioactive compounds, such as phenolic, flavonoids, and alkamides. The review focused on the summarization of in vitro and in vivo experimental reports on the antioxidant and anti-inflammatory actions of S. acmella, as well as how they contributed to potential health benefits in lowering the risk of diseases that were related to oxidative stress. The molecular mechanism of S. acmella in reducing oxidative stress and inflammatory targets, such as inducible nitric oxide synthase (iNOS), transcription factors of the nuclear factor-κB family (NF-κB), cyclooxygenase-2 (COX-2), and mitogen-activated protein kinase (MAPK) signaling pathways were discussed. Besides, the antioxidant potential of S. acmella was measured by total phenolic content (TPC), total flavonid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and superoxide anion radical scavenging (SOD) and thiobarbituric acid reactive substance (TBARS) assays. This review revealed that S. acmella might have a potential role as a reservoir of bioactive agents contributing to the observed antioxidant, anti-inflammatory, and health beneficial effects.

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NME3 Regulates Mitochondria to Reduce ROS-Mediated Genome Instability

International Journal of Molecular Sciences ◽

10.3390/ijms21145048 ◽

2020 ◽

Vol 21 (14) ◽

pp. 5048

Author(s):

Chih-Wei Chen ◽

Ning Tsao ◽

Wei Zhang ◽

Zee-Fen Chang

Keyword(s):

Oxidative Stress ◽

Dna Repair ◽

Genome Stability ◽

Nuclear Dna ◽

Genome Instability ◽

Mitochondrial Fission ◽

Nucleoside Diphosphate Kinase ◽

Mitochondrial Fusion ◽

Mitochondrial Outer Membrane ◽

Mitochondrial Oxidative Stress

NME3 is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. In this study, we showed that NME3 knockdown delayed DNA repair without reducing the cellular levels of nucleotide triphosphates. Further analyses revealed that NME3 knockdown increased fragmentation of mitochondria, which in turn led to mitochondrial oxidative stress-mediated DNA single-strand breaks (SSBs) in nuclear DNA. Re-expression of wild-type NME3 or inhibition of mitochondrial fission markedly reduced SSBs and facilitated DNA repair in NME3 knockdown cells, while expression of N-terminal deleted mutant defective in mitochondrial binding had no rescue effect. We further showed that disruption of mitochondrial fusion by knockdown of NME4 or MFN1 also caused mitochondrial oxidative stress-mediated genome instability. In conclusion, the contribution of NME3 to redox-regulated genome stability lies in its function in mitochondrial fusion.

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p21Cip1/WAF1/Sdi1Does Not Affect Expression of Base Excision DNA Repair Enzymes During Chronic Oxidative Stress

Antioxidants and Redox Signaling ◽

10.1089/ars.2005.7.719 ◽

2005 ◽

Vol 7 (5-6) ◽

pp. 719-725 ◽

Cited By ~ 2

Author(s):

Michael A. O'Reilly ◽

Peter F. Vitiello ◽

Sean C. Gehen ◽

Rhonda J. Staversky

Keyword(s):

Oxidative Stress ◽

Dna Repair ◽

Dna Repair Enzymes ◽

Repair Enzymes ◽

Chronic Oxidative Stress ◽

Affect Expression ◽

Base Excision ◽

Base Excision Dna Repair

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Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells

Biomolecules ◽

10.3390/biom11010087 ◽

2021 ◽

Vol 11 (1) ◽

pp. 87

Author(s):

Francesca Perut ◽

Laura Roncuzzi ◽

Sofia Avnet ◽

Annamaria Massa ◽

Nicoletta Zini ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Mammalian Cells ◽

Dependent Manner ◽

Potential Health ◽

Human Mesenchymal Stromal Cells ◽

Health Promoting ◽

Size And Morphology

Plant-derived exosome-like nanovesicles (EPDENs) have recently been isolated and evaluated as potential bioactive nutraceutical biomolecules. It has been hypothesized that EPDENs may exert their activity on mammalian cells through their specific cargo. In this study, we isolated and purified EPDENs from the strawberry juice of Fragaria x ananassa (cv. Romina), a new cultivar characterized by a high content of anthocyanins, folic acid, flavonols, and vitamin C and an elevated antioxidant capacity. Fragaria-derived EPDENs were purified by a series of centrifugation and filtration steps. EPDENs showed size and morphology similar to mammalian extracellular nanovesicles. The internalization of Fragaria-derived EPDENs by human mesenchymal stromal cells (MSCs) did not negatively affect their viability, and the pretreatment of MSCs with Fragaria-derived EPDENs prevented oxidative stress in a dose-dependent manner. This is possibly due to the presence of vitamin C inside the nanovesicle membrane. The analysis of EPDEN cargo also revealed the presence of small RNAs and miRNAs. These findings suggest that Fragaria-derived EPDENs may be considered nanoshuttles contained in food, with potential health-promoting activity.

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