scholarly journals Distribution Characteristics of Per- and Polyfluoroalkyl Substances (PFASs) in Human Urines of Acrylic Fiber Plant and Chemical Plant

2021 ◽  
Author(s):  
Lin Peng ◽  
Wang Xu ◽  
Qinghuai Zeng ◽  
Yao Cheng ◽  
Yingjie Zhang ◽  
...  
Keyword(s):  
Chemical Plant ◽  
Urine Samples ◽  
Short Chain ◽  
Distribution Characteristics ◽  
Low Frequencies ◽  
Acrylic Fiber ◽  
Detection Frequency ◽  
Fiber Plant ◽  
Polyfluoroalkyl Substances ◽  
And Gender

Abstract Per- and polyfluoroalkyl substances (PFASs) are persistent and bio-accumulative substances that have many adverse effects on human bodies. This study investigated the PFASs distribution characteristics in urine samples of workers from an acrylic fiber plant and a chemical plant. It was found that perfluorobutanoic acid (PFBA) was the predominant PFASs both in urine samples from the chemical plant (detection frequency: 86.52%; median value: 39.01 ng/mL) and the acrylic fiber plant (detection frequency: 88.16%; median value: 44.36 ng/mL). Meanwhile, perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) were detected with very low frequencies and low concentrations. Furthermore, the results showed that PFASs levels in urine samples of workers from different units of the plants were quite different. PFASs concentrations of urine samples in males were higher than those in females, especially for PFBA, PFHxA, and PFDoA. The age had limited effects on the PFASs distribution in urine samples in this study, as short-chain PFASs were the dominant compounds. The correlations between PFASs concentrations in urine and gender/ages of workers were finally analyzed by Person correlation. The overall results may indicate that short-chain PFASs (such as: PFBA and PFBS) were becoming dominant for human exposure, especially occupational workers.

scholarly journals 979. Disparities in PrEP uptake and adherence among cisgender women using a pharmacologic measure

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S517-S518
Author(s):  
Shane Hebel ◽  
Elijah Kahn-Woods ◽  
Casper Enghuus ◽  
Helen Koenig ◽  
Linden Lalley-Chareczko ◽  
...  
Keyword(s):  
Real World ◽  
Minority Groups ◽  
Hiv Infections ◽  
Urine Samples ◽  
Real World Data ◽  
Women And Gender ◽  
Gender Minority ◽  
Adherence Data ◽  
And Gender ◽  
Exposure Prophylaxis

Abstract Background HIV pre-exposure prophylaxis (PrEP) is 99% effective at preventing new HIV infections if taken daily. To be successful, PrEP requires concurrent efforts to optimize uptake, persistence, and adherence. In 2018, cisgender (cis) women accounted for 19% of new HIV infections in the US but comprised only 7% of all PrEP users. Studies show poor PrEP adherence amongst cis women, but there is a paucity of real-world clinical data describing PrEP adherence among cis women and gender minority people. Methods An adherence test that measures the concentration of tenofovir in urine samples using a liquid chromatography mass spectrometry (LC-MS/MS) was used to assess recent PrEP adherence at 8 clinics. Urine samples were collected during routine visits and analyzed using the LC-MS/MS assay. Test results were retrospectively paired with gender data, when available, and sex assigned at birth (SAAB) data. Adherence data were aggregated and analyzed to assess non-adherence proportions by sub-population. Results Gender data were available from 1,461 patients at 5 clinics, 1,344 (92%) of whom were cis males (Figure 1). From the 5 clinics where gender and SAAB data were available, 3,835 tests were conducted and 517 (13.5%) indicated non-adherence (Figure 2). 3 additional clinics conduct routine adherence testing and collect SAAB data (gender data not available). At these 8 clinics, SAAB data were available for 2,773 PrEP patients, totaling 5,602 urine tests (Figure 3). Among these 5,602 adherence tests, 813 (14.5%) indicated non-adherence (Figure 4). SAAB females demonstrated significantly higher non-adherence than SAAB males (22% vs 14%, p< 0.001). Across clinics, 89%-98% of PrEP patients are SAAB male (Figure 5). Within these 8 clinics, SAAB female demonstrated consistently higher non-adherence (17%-44%, vs 12%-17% for SAAB males) (Figure 6). Figures 1 and 2 Figures 3 and 4 Figures 5 and 6 Conclusion Real-world data align with nationwide trends in PrEP utilization and show that the majority of PrEP patients are cis men. When initiated on PrEP, cis women exhibit higher rates of non-adherence than cis men. These data underscore the need to collect gender-identity data to monitor PrEP disparities and suggest that greater efforts are needed to target PrEP access, utilization, and accompanying support services to cis women and gender minority groups. Disclosures All Authors: No reported disclosures

scholarly journals Per- and polyfluoroalkyl substances in human serum and urine samples from a residentially exposed community

2017 ◽  
Vol 106 ◽  
pp. 135-143 ◽  
Author(s):  
Rachel Rogers Worley ◽  
Susan McAfee Moore ◽  
Bruce C. Tierney ◽  
Xiaoyun Ye ◽  
Antonia M. Calafat ◽  
...  
Keyword(s):  
Human Serum ◽  
Urine Samples ◽  
Polyfluoroalkyl Substances ◽  
Serum And Urine ◽  
Serum And Urine Samples

scholarly journals On the Ability of Perfluorohexane Sulfonate (PFHxS) Bioaccumulation by Two Pseudomonas sp. Strains Isolated from PFAS-Contaminated Environmental Matrices

2020 ◽  
Vol 8 (1) ◽  
pp. 92 ◽  
Author(s):  
Alessandro Presentato ◽  
Silvia Lampis ◽  
Andrea Vantini ◽  
Flavio Manea ◽  
Francesca Daprà ◽  
...  
Keyword(s):  
Short Chain ◽  
Industrial Processes ◽  
Bacterial Cells ◽  
Pseudomonas Sp ◽  
Environmental Matrices ◽  
Synthetic Compounds ◽  
Physiological Conditions ◽  
End Products ◽  
Polyfluoroalkyl Substances ◽  
Thermal And Chemical Stability

PFASs (perfluoroalkyl and polyfluoroalkyl substances) are highly fluorinated, aliphatic, synthetic compounds with high thermal and chemical stability as well as unique amphiphilic properties which make them ingredients in a range of industrial processes. PFASs have attracted consideration due to their persistence, toxicity and bioaccumulation tendency in the environment. Recently, attention has begun to be addressed to shorter-chain PFASs, such as perfluorohexane sulfonate [PFHxS], apparently less toxic to and more easily eliminated from lab animals. However, short-chain PFASs represent end-products from the transformation of fluorotelomers whose biotic breakdown reactions have not been identified to date. This means that such emergent pollutants will tend to accumulate and persist in ecosystems. Since we are just learning about the interaction between short-chain PFASs and microorganisms, this study reports on the response to PFHxS of two Pseudomonas sp. strains isolated from environmental matrices contaminated by PFASs. The PFHxS bioaccumulation potential of these strains was unveiled by exploiting different physiological conditions as either axenic or mixed cultures under alkanothrofic settings. Moreover, electron microscopy revealed nonorthodox features of the bacterial cells, as a consequence of the stress caused by both organic solvents and PFHxS in the culturing substrate.

scholarly journals A Comparative Study of Spatial and Temporal Preferences for Waterfronts in Wuhan based on Gender Differences in Check-In Behavior

2019 ◽  
Vol 8 (9) ◽  
pp. 413 ◽  
Author(s):  
Wu ◽  
Li ◽  
Ma
Keyword(s):  
Gender Differences ◽  
Social Media ◽  
Geographical Location ◽  
Distribution Characteristics ◽  
Social Media Data ◽  
Men And Women ◽  
Male And Female ◽  
Points Of Interest ◽  
And Gender ◽  
Media Data

The geographical location and check-in frequency of social platform users indicate their personal preferences and intentions for space. On the basis of social media data and gender differences, this study analyzes Weibo users’ preferences and the reasons behind these preferences for the waterfronts of the 21 major lakes within Wuhan’s Third Ring Road, in accordance with users’ check-in behaviors. According to the distribution characteristics of the waterfronts’ points of interest, this study explores the preferences of male and female users for waterfronts and reveals, through the check-in behaviors of Weibo users, the gender differences in the preference and willingness of these users to choose urban waterfronts. Results show that men and women check in significantly more frequently on weekends than on weekdays. Women are more likely than men to check in at waterfronts. Significant differences in time and space exist between male and female users’ preferences for different lakes.

scholarly journals Reliability of studies of iodine intake and recommendations for number of samples in groups and in individuals

2007 ◽  
Vol 99 (4) ◽  
pp. 813-818 ◽  
Author(s):  
Stig Andersen ◽  
Jesper Karmisholt ◽  
Klaus M. Pedersen ◽  
Peter Laurberg
Keyword(s):  
Standard Deviation ◽  
Urinary Iodine ◽  
Iodine Intake ◽  
Urine Samples ◽  
Urinary Iodine Excretion ◽  
Iodine Nutrition ◽  
Cross Sectional ◽  
Spot Urine ◽  
Iodine Excretion ◽  
And Gender

The iodine intake level in a population is determined in cross-sectional studies. Urinary iodine varies considerably and the reliability of studies of iodine nutrition and the number of samples needed is unsettled. We performed a longitudinal study of sixteen healthy men living in an area of mild to moderate iodine deficiency. Iodine and creatinine concentrations were measured in spot urine samples collected monthly for 13 months. From these data we calculated the number of urine samples needed to determine the iodine excretion level for crude urinary iodine and for 24 h iodine excretion estimated from age- and gender-specific creatinine excretions. We found that mean urinary iodine excretion varied from 30 to 87 μg/l (31 to 91 μg/24 h). Sample iodine varied from 10 to 260 μg/l (20 to 161 μg/24 h). Crude urinary iodine varied more than estimated 24 h iodine excretion (population standard deviation 32v. 26; individual standard deviation 29v. 21; Bartlett's test,P < 0·01 for both). The number of spot urine samples needed to estimate the iodine level in a population with 95 % confidence within a precision range of ± 10 % was about 125 (100 when using estimated 24 h iodine excretions), and within a precision range of ± 5 % was about 500 (400). A precision range of ± 20 % in an individual required twelve urine samples or more (seven when using estimated 24 h iodine excretions). In conclusion, estimating population iodine excretion requires 100–500 spot urine samples for each group or subgroup. Less than ten urine samples in an individual may be misleading.

Detection of low-frequency FGFR3 mutations in the urine of patients with bladder cancer using next-generation deep sequencing.

2012 ◽  
Vol 30 (30_suppl) ◽  
pp. 59-59
Author(s):  
John M. Millholland ◽  
Shuqiang Li ◽  
Cecilia A. Fernandez ◽  
Anthony P. Shuber
Keyword(s):  
Bladder Cancer ◽  
Deep Sequencing ◽  
Amplicon Sequencing ◽  
Urine Samples ◽  
Next Generation ◽  
High Background ◽  
Next Generation Sequencing Technology ◽  
Non Invasive ◽  
Detection Frequency ◽  
Fgfr3 Mutations

59 Background: FGFR3 mutations have been identified in ~60-70% of low-stage, non-invasive tumors. Our group and others have developed assays to detect FGFR3 mutations in the urine of bladder cancer patients. However, urine-based assays have been limited by the technical ability to detect rare events in a dilute medium where there is a high background of normal DNA. In these assays, FGFR3 mutations are generally found in ~30% of the urine samples, which is < 50% concordance with the expected detection in tissue. We have now developed an ultra-deep amplicon sequencing technique that increases FGFR3 mutation detection in urine to ~67%, close to the expected detection frequency if every mutation found in tissue could be detected in urine. Methods: Amplicons were designed against FGFR3 exons 7, 10, and 15 using PCR primers containing the adapter sequences for unidirectional sequencing. Primary amplification was performed from DNA isolated from 4 ml of urine. The resulting PCR products were used as template for emulsion PCR and these were then sequenced using the Roche 454 GS Junior. Samples were analyzed for total DNA reads per sample and number of mutant sequencing reads to determine percent mutation. Results: Urine samples from 43 patients with bladder cancer were analyzed by both our previously described qPCR method and the new ultra-deep sequencing approach. Using ultra-deep amplicon sequencing, 24 out of 43 (55.8%) were positive for FGFR3 mutations, while only 5 out of 43 (11.6%) were positive for mutations by qPCR. The urine samples from the 15 newly identified mutations using deep sequencing contained FGFR3 mutations as low as 0.05% mutant DNA. The sensitivity achieved using deep sequencing was 91% concordant with the FGFR3 mutations observed in tissue. Conclusions: We have developed a highly sensitive non-invasive urine based assay that can detect FGFR3 mutant DNA when present at < 1% of the sample and is > 90% concordance with the mutations found in tumor tissues. To our knowledge, this is the first practical application of next generation sequencing technology for diagnostic use.

scholarly journals Short-chain per- and polyfluoroalkyl substances in aquatic systems: Occurrence, impacts and treatment

2020 ◽  
Vol 380 ◽  
pp. 122506 ◽  
Author(s):  
Fan Li ◽  
Jun Duan ◽  
Shuting Tian ◽  
Haodong Ji ◽  
Yangmo Zhu ◽  
...  
Keyword(s):  
Aquatic Systems ◽  
Short Chain ◽  
Polyfluoroalkyl Substances
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