scholarly journals Enhanced Reduction of Polymicrobial Biofilms on the Orthodontic Brackets and Enamel Surface Remineralization Using Zeolite-Zinc Oxide Nanoparticles-Based Antimicrobial Photodynamic Therapy

2021 ◽  
Author(s):  
Maryam Pourhajibagher ◽  
Abbas Bahador
Keyword(s):  
Zinc Oxide ◽  
Photodynamic Therapy ◽  
Zinc Oxide Nanoparticles ◽  
Enamel Surface ◽  
Orthodontic Brackets ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Antimicrobial Photodynamic Therapy ◽  
Diagnodent Pen ◽  
Metabolic Activities

Abstract The aim of this study was to evaluate the anti-biofilm and anti-metabolic activities of zeolite-zinc oxide nanoparticles (Zeo\ZnONPs)-based antimicrobial photodynamic therapy (aPDT) against pre-formed polymicrobial biofilms on the orthodontic brackets, as well as, assess the remineralization efficacy on polymicrobial biofilms induced enamel lesions. Following synthesis and characterization of Zeo\ZnONPs, cell cytotoxicity, hemolytic effect, and intracellular reactive oxygen species (ROS) production were determined. The anti-biofilm and anti-metabolic activities of aPDT using different concentrations of Zeo\ZnONPs were investigated. Microhardness tester and DIAGNOdent Pen were used to evaluate the changes of remineralization degree on the treated enamel slabs duration one and three months. No significant cytotoxicity and erythrocyte hemolysis were observed in treated cells with Ze\ZnONPs. When irradiated, suggesting that the Ze\ZnONPs were photoactivated, generating ROS and leading to reduce dose-dependently the cell viability and metabolic activity of polymicrobial biofilms. Also, the enamel surface microhardness value of exposed enamel showed a steady increase with the concentration of Zeo\ZnONPs. No statistically significant differences were shown between aPDT and sodium fluoride varnish as the control group. Overall, Zeo\ZnONPs-based aPDT with the greatest remineralization efficacy of enamel surface can be used as an anti-biofilm therapeutic method, which is involved with their potent ability to produce ROS.

scholarly journals Enhanced Reduction of Polymicrobial Biofilms on the Orthodontic Brackets and Enamel Surface Remineralization Using Zeolite-Zinc Oxide Nanoparticles- Based Antimicrobial Photodynamic Therapy

2021 ◽  
Author(s):  
Maryam Pourhajibagher ◽  
Abbas Bahador
Keyword(s):  
Zinc Oxide ◽  
Photodynamic Therapy ◽  
Zinc Oxide Nanoparticles ◽  
Enamel Surface ◽  
Orthodontic Brackets ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Antimicrobial Photodynamic Therapy ◽  
Diagnodent Pen ◽  
Metabolic Activities

Abstract The aim of this study was to evaluate the anti-biofilm and anti-metabolic activities of zeolite-zinc oxide nanoparticles (Zeo/ZnONPs)-based antimicrobial photodynamic therapy (aPDT) against pre-formed polymicrobial biofilms on the orthodontic brackets, as well as, assess the remineralization efficacy on polymicrobial biofilms induced enamel lesions. Following synthesis and characterization of Zeo/ZnONPs, cell cytotoxicity, hemolytic effect, and intracellular reactive oxygen species (ROS) production were determined. The anti-biofilm and anti-metabolic activities of aPDT using different concentrations of Zeo/ZnONPs were investigated. Microhardness tester and DIAGNOdent Pen were used to evaluate the changes of remineralization degree on the treated enamel slabs duration one and three months. No significant cytotoxicity and erythrocyte hemolysis were observed in treated cells with Ze/ZnONPs. When irradiated, suggesting that the Ze/ZnONPs were photoactivated, generating ROS and leading to reduce dose-dependently the cell viability and metabolic activity of polymicrobial biofilms. Also, the enamel surface microhardness value of exposed enamel showed a steady increase with the concentration of Zeo/ZnONPs. No statistically significant differences were shown between aPDT and sodium fluoride varnish as the control group. Overall, Zeo/ZnONPs-based aPDT with the greatest remineralization efficacy of enamel surface can be used as an anti-biofilm therapeutic method, which is involved with their potent ability to produce ROS.

scholarly journals Enhanced reduction of polymicrobial biofilms on the orthodontic brackets and enamel surface remineralization using zeolite-zinc oxide nanoparticles-based antimicrobial photodynamic therapy

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Maryam Pourhajibagher ◽  
Abbas Bahador
Keyword(s):  
Zinc Oxide ◽  
Photodynamic Therapy ◽  
Zinc Oxide Nanoparticles ◽  
Enamel Surface ◽  
Orthodontic Brackets ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Antimicrobial Photodynamic Therapy ◽  
Diagnodent Pen ◽  
Metabolic Activities

AbstractThe aim of this study was to evaluate the anti-biofilm and anti-metabolic activities of zeolite-zinc oxide nanoparticles (Zeo/ZnONPs)-based antimicrobial photodynamic therapy (aPDT) against pre-formed polymicrobial biofilms on the orthodontic brackets, as well as, assess the remineralization efficacy on polymicrobial biofilms induced enamel lesions. Following synthesis and characterization of Zeo/ZnONPs, cell cytotoxicity, hemolytic effect, and intracellular reactive oxygen species (ROS) production were determined. The anti-biofilm and anti-metabolic activities of aPDT using different concentrations of Zeo/ZnONPs were investigated. Microhardness tester and DIAGNOdent Pen were used to evaluate the changes of remineralization degree on the treated enamel slabs duration 1 and 3 months. No significant cytotoxicity and erythrocyte hemolysis were observed in treated cells with Zeo/ZnONPs. When irradiated, suggesting that the Zeo/ZnONPs were photoactivated, generating ROS and leading to reduce dose-dependently the cell viability and metabolic activity of polymicrobial biofilms. Also, the enamel surface microhardness value of exposed enamel showed a steady increase with the concentration of Zeo/ZnONPs. No statistically significant differences were shown between aPDT and sodium fluoride varnish as the control group. Overall, Zeo/ZnONPs-based aPDT with the greatest remineralization efficacy of enamel surface can be used as an anti-biofilm therapeutic method, which is involved with their potent ability to produce ROS.

scholarly journals Zinc Oxide Nanoparticles and Photodynamic Therapy for the Treatment of B-chronic Lymphocytic Leukemia

10.5772/61796 ◽  
2015 ◽  
Author(s):  
Sandra Loydover Peña Luengas ◽  
Gustavo H. Marin ◽  
Luis Rivera ◽  
Adrian Tarditti ◽  
Gustavo Roque ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Photodynamic Therapy ◽  
Chronic Lymphocytic Leukemia ◽  
Zinc Oxide Nanoparticles ◽  
Lymphocytic Leukemia ◽  
Oxide Nanoparticles

PEGylated silver doped zinc oxide nanoparticles as novel photosensitizers for photodynamic therapy against Leishmania

2014 ◽  
Vol 77 ◽  
pp. 230-238 ◽  
Author(s):  
Akhtar Nadhman ◽  
Samina Nazir ◽  
Malik Ihsanullah Khan ◽  
Syeda Arooj ◽  
Muhammad Bakhtiar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Photodynamic Therapy ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Investigation of sub-acute levels of zinc oxide nanoparticles on the filtration rate of Mytilaster lineatus and Dressina polymorpha in the short term

2018 ◽  
Vol 10 (1) ◽  
pp. 53-57
Author(s):  
AMIR QADERMARZI ◽  
MOJTABA POULADI ◽  
ALI REZAMAND ◽  
SEYED HOSSEIN HOSEINIFAR ◽  
ALI AKBAR HEDAYATI
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Filtration Rate ◽  
Infiltration Rate ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Short Term ◽  
The Third ◽  
Significant Difference ◽  
Mytilaster Lineatus

Qadermarzi A, Pouladi M, Rezamand A, Hoseinifar SH, Hedayati AA. 2018. Investigation of sub-acute levels of zinc oxide nanoparticles on the filtration rate of Mytilaster lineatus and Dressina polymorpha in the short term. Nusantara Bioscience 10: 53-57. The filtration rate is one of the physiological indices in the bivalves which indicate the degree of efficacy of the filtration function in the exposure to contaminants. In this study, changes in the filtration rate of freshwater bivalves (D. polymorpha and M. lineatus) were investigated after exposure periods of 5 and 10 days with nanoparticles. Bivalves were transferred from the natural environment to the laboratory. The distribution of nanoparticles was measured by differential light scattering (DLS). Bivalves were exposed to 2.5, 25 and 50 ppm nanoparticles for ten days in water reservoirs. The filtration rates were significantly different in the treatments compared to the control samples, with the highest filtration rate was observed in the control group. On the fifth day, the highest filtration rate was obtained in the first treatment of M. lineatus and the lowest filtration rate was obtained in the third treatment of D. polymorphia. The highest filtration rates were observed in the first and second treatments and the lowest filtration rate was observed in the third treatment. Also, there was no significant difference in the filtration rate of M. lineatus in the 1st treatment with control (P> 0.05) on the 5th day, but filtration rate was significantly (P <0.05) less than other concentrations and control group in the third treatment. On the other hand, the filtration rates on the 10th day showed similar differences compared to the 5th day. It could be concluded that the changes infiltration rate are an appropriate indicator for the measurement of contamination in nanoparticles.

The Interaction of Zinc Oxide/Green Tea Extract Complex Nanoparticles and its Effect on Monosodium Glutamate Toxicity in Liver of Rats

2019 ◽  
Vol 20 (6) ◽  
pp. 465-475 ◽  
Author(s):  
Fawziah A. Al-Salmi ◽  
Reham Z. Hamza ◽  
Nahla S. El-Shenawy
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Green Tea Extract ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are increasingly utilized in both industrial and medical applications. Therefore, the study was aimed to investigate the effect of green nanoparticle complex (green tea extract/zinc oxide nanoparticles complex, GTE/ZnO NPs) on oxidative stress induced by monosodium glutamate (MSG) on the liver of rats. Methods: Wistar male rats (n=64) weighing between 200-250 g were divided randomly into eight groups: control group was given physiological saline (1 mg/kg), two groups were treated with two different doses of MSG (MSG-LD, MSG-HD; 6 and 17.5 mg/Kg, respectively), GTE was given 1 mg/mL, 5th group was treated with ZnO NPs and 6th group was treated with GTE/ZnO NPs complex while, 7th and 8th groups were treated with MSG-LD + GTE/ZnO NPs complex and MSG-HD + GTE/ZnO NPs complex, respectively. All substances were given orally for 30 consecutive days. At the end of the study, the liver was homogenized for measurement of the oxidative stress status and anti-inflammatory biomarkers as well as histological and transmission alternations. Results: Results showed that the antioxidant enzymes activity and glutathione level were significantly decreased in MSG groups than control in a dose-dependent manner. Conversely, the malondialdehyde and inflammatory cytokines levels were significantly increased in MSG groups than the control group. The liver indicated no evidence of alteration in oxidative status, anti-inflammatory and morphological parameters in GTE, ZnO NPs and GTE/ZnO NPs complex groups. Conclusion: In conclusion, MSG at both doses caused oxidative stress and inflammation on liver after 28 days of exposure that supported histological analysis and transmission view of hepatic parenchyma. GTE/ZnO NPs act as partial hepato-protective against MSG.

scholarly journals Zinc Oxide Nanoparticles Protected with Terpenoids as a Substance in Redox Imbalance Normalization in Burns

2021 ◽  
Vol 14 (6) ◽  
pp. 492
Author(s):  
Nina Melnikova ◽  
Alyona Balakireva ◽  
Dmitry Orekhov ◽  
Denis Kamorin ◽  
Natalia Didenko ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Hysteresis Loops ◽  
Fold Increase ◽  
Redox Balance ◽  
Healing Time ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Ethanol Medium

Preliminary protection of zinc oxide nanoparticles (ZnO NPs) with terpenoids such as betulin, its derivatives, and essential oils components has been proposed to produce gel-like oleophilic and hydrophilic formulations. We studied the properties of gel-like dispersions of ZnO NPs with immobilized terpenoids and their effects on the activity of LDH, GR, G6PDH, restoration of redox balance of co-enzyme pairs NAD+/NADH and NADP+/NADPH, as well as the activity of SOD, catalase, AlDH in erythrocytes in the treatment of burns in rats. Hysteresis loops on the rheograms of studied dispersions characterize their thixotropic properties. ZnO NPs with betulin diphosphate in the water–ethanol medium lead to a 20-fold increase in the hydrodynamic radius at pH 7.3 compared to the initial ZnO NPs, and facilitate the formation of Zn2+ ions and their penetration into the viable epidermis, unlike oleophilic dispersions. All dispersions reduce the healing time by one and a half times compared with the untreated control group, increase the activity of LDH, GR, G6PDH, SOD, catalase, AlDH, and contribute to the normalization of coenzyme balance. Normalization of the redox balance and wound state was more effective using hydrophilic dispersions due to Zn2 + penetration.

scholarly journals Zinc Oxide Nanoparticles and Zinc Sulfate Impact Physiological Parameters and Boosts Lipid Peroxidation in Soil Grown Coriander Plants (Coriandrum sativum)

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1998
Author(s):  
Norma Ruiz-Torres ◽  
Antonio Flores-Naveda ◽  
Enrique Díaz Barriga-Castro ◽  
Neymar Camposeco-Montejo ◽  
Sonia Ramírez-Barrón ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Zinc Sulfate ◽  
Atomic Emission ◽  
Coriandrum Sativum ◽  
Emission Spectrometry ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Chlorophyll Accumulation

The objective of this study was to determine the oxidative stress and the physiological and antioxidant responses of coriander plants (Coriandrum sativum) grown for 58 days in soil with zinc oxide nanoparticles (ZnO NPs) and zinc sulfate (ZnSO4) at concentrations of 0, 100, 200, 300, and 400 mg of Zn/kg of soil. The results revealed that all Zn compounds increased the total chlorophyll content (CHLt) by at least 45%, compared to the control group; however, with 400 mg/kg of ZnSO4, chlorophyll accumulation decreased by 34.6%. Zn determination by induction-plasma-coupled atomic emission spectrometry (ICP–AES) showed that Zn absorption in roots and shoots occurred in plants exposed to ZnSO4 at all concentrations, which resulted in high levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA). Only at 400 mg/kg of ZnSO4, a 78.6% decrease in the MDA levels was observed. According to the results, the ZnSO4 treatments were more effective than the ZnO NPs to increase the antioxidant activity of catalase (CAT), ascorbate peroxidase (APX), and peroxidases (POD). The results corroborate that phytotoxicity was higher in plants subjected to ZnSO4 compared to treatments with ZnO NPs, which suggests that the toxicity was due to Zn accumulation in the tissues by absorbing dissolved Zn++ ions.

scholarly journals The Effects of Pentoxifylline on Memory in Male Rats Treated with Zinc Oxide NPs

2021 ◽  
Vol 25 (1) ◽  
pp. 1-10
Author(s):  
Niloufar Darbandi ◽  
◽  
Zeynab Vasheghani Farahani ◽  
Hamidreza Momeni ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Treatment Group ◽  
Blood Serum ◽  
Zinc Oxide Nanoparticles ◽  
Male Rats ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Ca1 Region

Background: Zinc oxide Nanoparticles (NPs) present irreversible effects on the nervous system, memory, and learning. Objective: The current study aimed to investigate the effects of pentoxifylline on memory impairments, CA1 hippocampal pyramidal cells, and blood serum antioxidant enzymes in male rats treated with zinc oxide NPs. Methods: Male Wistar rats were divided into the control, zinc oxide NPs (1.25 mg/kg), pentoxifylline (50 mg/kg), and pentoxifylline with zinc oxide NPs groups. In all study groups, saline, zinc oxide NPs, and pentoxifylline were intraperitoneally injected 30 minutes before training. In the co-treatment group, pentoxifylline was injected one hour before injecting Zno NPs. After performing the behavioral test, the tested animals’ brains were fixed and the number of healthy neurons in the CA1 region of the hippocampus was counted. In all research groups, malondialdehyde levels, total antioxidant power, superoxide dismutase levels, and glutathione peroxidase in blood serum were measured. Results: Zinc oxide nanoparticles decreased memory and the number of healthy neurons in the CA1 region of the hippocampus and increased oxidative stress in blood serum, compared to the controls. In the co-treatment group, using pentoxifylline improved the above-mentioned factors and reached the level of the control group. Pentoxifylline alone presented no significant effect on the aforementioned characteristics, compared to the control group. Conclusion: ZnO NPs may decrease memory retrieval and cause cell death in the pyramidal neurons of the CA1 region of the hippocampus by increasing oxidative stress. Pentoxifylline, as a potent antioxidant, can prevent the harmful effects of ZnO NPs.

Synthesis, characterization, and pharmacological evaluation of zinc oxide nanoparticles formulation

2018 ◽  
Vol 34 (11) ◽  
pp. 753-763 ◽  
Author(s):  
Zulfia Hussain ◽  
Junaid Ali Khan ◽  
Hafeez Anwar ◽  
Naila Andleeb ◽  
Sehrish Murtaza ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Antimicrobial Properties ◽  
Average Crystallite Size ◽  
Precipitation Method ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
In Vivo Evaluation ◽  
Pharmaceutical Industries

Zinc oxide nanoparticles (ZnONPs) are being used extensively in manufacturing skin lotions and food products and in various biological and pharmaceutical industries because of their immunomodulatory and antimicrobial properties. In this study, ZnONPs were synthesized by a precipitation method and characterized by X-ray diffraction (XRD) techniques, scanning electron microscopy (SEM), and ultraviolet–visible spectroscopy to investigate their structural, morphological, and optical properties. For in vivo evaluation, 40 healthy albino mice were randomly allocated to four equal groups among which the first one was the control group, while the second, third, and fourth were treated with carbon tetrachloride (CCl4), a blend of CCl4 and ZnONPs, and ZnONPs alone, respectively, for 21 days. The XRD analysis confirmed hexagonal wurtzite type structures having an average crystallite size of 41.54 nm. The morphology of ZnONPs analyzed through SEM showed uniform distribution of the grains and shape of the synthesized oxide. The energy band gap of the ZnONPs was found to be 3.498 eV. Hepatic and renal damage following CCl4 administration was apparent after 14 days and was increased at the 21st day, showing nodular fibrotic masses in the liver and bumpy surfaces in the kidney as observed by gross and histological examination. Coadministration of ZnONPs (15 mg/kg b.w. intragastrically 5 days a week) significantly prevented the CCl4-dependent increases in alanine transaminase, aspartate transaminase, creatinine, and urea levels, suggesting a protective potential of ZnONPs.

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