scholarly journals Potential of Aspergilus Niger MT809753 for Bio-treatment Paper Industry Wastewater After Screening Effective Factors on Production of Cellulase Enzyme by Plackett-burman Design

2021 ◽  
Author(s):  
Marwa El-Sesy ◽  
Amira M Aly
Keyword(s):  
Rice Straw ◽  
Enzyme Production ◽  
Paper Industry ◽  
Cellulase Production ◽  
Barley Straw ◽  
Cellulase Enzyme ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Aspergilus Niger ◽  
Industry Wastewater

Abstract A microorganism capable of degrading cellulose present in rice straw was isolated from wastewater samples and identified as Aspergilus niger MT809753 by 18S rDNA. In the present study various cheap agronomic cellulosic wastes as (cotton seed husks, barley straw, rice straw and maize straw) were utilized as crude inducers for the cellulase enzyme production and represent the carbon source for isolates where cellulose activity was measured by (DNS) method. The highest cellulases enzyme production was obtained by fungal isolate Aspergilus niger MT809753 within 24 hours (0.532 IU/ml) using rice straw. Plackett-Burman design was used as conventional method for statistically screening of different variables. Nine variables of the production process were selected. The results illustrate those seven variables, namely as (inoculum size, substrate concentration, incubation temperature, pH, shaking conditions, and incubation time and peptone concentration) had influence with high confidence levels, while the remaining two variables did not show a significant effect on cellulase production. After using response optimization the experiment was performed and the obtained cellulase production was 1.08 IU/ml. A bench scale study was performed to examine paper industry wastewater treatment efficiency by Aspergillus Niger MT809753. Results reveal that organisms have proved their bioremediation potency in treatment of paper industry effluent. The importance of the research stems from the fact that it sheds light on the role of some fungi in the production of the cellulase enzyme. So our goal is to obtain local isolates from fungi having a high ability to produce the cellulase enzyme, as well as developing an effective treatment processes to get rid of environmental cellulosic pollution and utilization of cellulosic wastes as cheap carbon sources.

scholarly journals Cellulase Enzyme Production from Filamentous Fungi Trichoderma reesei and Aspergillus awamori in Submerged Fermentation with Rice Straw

2021 ◽  
Vol 7 (10) ◽  
pp. 868
Author(s):  
Laila Naher ◽  
Siti Noor Fatin ◽  
Md Abdul Halim Sheikh ◽  
Lateef Adebola Azeez ◽  
Shaiquzzaman Siddiquee ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Filamentous Fungi ◽  
Rice Straw ◽  
Submerged Fermentation ◽  
Enzyme Production ◽  
Dry Weight ◽  
Cellulase Production ◽  
Fungal Species ◽  
Aspergillus Awamori ◽  
Cellulase Enzyme

Fungi are a diverse group of microorganisms that play many roles in human livelihoods. However, the isolation of potential fungal species is the key factor to their utilization in different sectors, including the enzyme industry. Hence, in this study, we used two different fungal repositories—soil and weed leaves—to isolate filamentous fungi and evaluate their potential to produce the cellulase enzyme. The fungal strains were isolated using dichloran rose bengal agar (DRBA) and potato dextrose agar (PDA). For cellulase enzyme production, a rice straw submerged fermentation process was used. The enzyme production was carried out at the different incubation times of 3, 5, and 7 days of culture in submerged conditions with rice straw. Fungal identification studies by morphological and molecular methods showed that the soil colonies matched with Trichoderma reesei, and the weed leaf colonies matched with Aspergillus awamori. These species were coded as T. reesei UMK04 and A. awamori UMK02, respectively. This is the first report of A. awamori UMK02 isolation in Malaysian agriculture. The results of cellulase production using the two fungi incorporated with rice straw submerged fermentation showed that T. reesei produced a higher amount of cellulase at Day 5 (27.04 U/mg of dry weight) as compared with A. awamori (15.19 U/mg of dry weight), and the concentration was significantly different (p < 0.05). Our results imply that T. reesei can be utilized for cellulase production using rice straw.

scholarly journals Optimization of Cellulase Enzyme Production by Co-cultures of Fungi Isolated from Lignocellulosic Waste

2020 ◽  
Vol 6 (5) ◽  
pp. 19-26
Author(s):  
Francis John V ◽  
Dr. Soloman P A
Keyword(s):  
Moisture Content ◽  
Incubation Time ◽  
Substrate Concentration ◽  
Enzyme Production ◽  
Trichoderma Viride ◽  
Cellulase Production ◽  
Optimal Conditions ◽  
Lignocellulosic Waste ◽  
Cellulase Enzyme ◽  
Degree Of Degradation

Fruit wastes were incubated with the mixture of cellulolytic fungi Penicillium citrinum, Aspergillus oryzae, and Trichoderma viride to hydrolyze the cellulosic components and to increase the degree of degradation. . The batch experiments are statistically designed and performed using Box-Benhken method of Response Surface Methodology to investigate the influence of major parameters viz., incubation time, temperature, pH, moisture content and substrate concentration on cellulase enzyme production. Maximum cellulase production of 2.03 Units/ml (U/ml) was detected by the RSM method in a mixed culture containing fungi at a ratio of 1: 1: 1 under optimal conditions at an incubation time of 5.27 days, a temperature of 34.09 °C, pH 4.85, moisture content of 63.83% and a substrate concentration of 5.03%.

scholarly journals OPTIMIZATION OF CELLULASE ENZYME PRODUCTION FROM Aspergillus oryzae FOR INDUSTRIAL APPLICATIONS

2017 ◽  
Vol 2 (2) ◽  
pp. 155 ◽  
Author(s):  
Hassan Sher ◽  
Muhammad Faheem ◽  
Abdul Ghani ◽  
Rashid Mehmood ◽  
Hamza Rehman ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Time Course ◽  
Value Added ◽  
Cellulase Production ◽  
Industrial Applications ◽  
Inoculum Size ◽  
Cellulosic Biomass ◽  
High Yield ◽  
Carboxymethyl Cellulase ◽  
Cellulase Enzyme

Cellulases are the hydrolytic group of enzymes, responsible for release of sugars in the bioconversion of the cellulosic biomass into a variety of value added industrial products. Fungal isolated cellulases are well studied and playing a significant role in various industrial processes. Enzymatic depolymerisation of cellulosic material has been done by the various fungal isolated enzymes. In the present study, the cultivation conditions for cellulase production from Aspergillus species were optimized. Optimization of scarification conditions such as time course, inoculum size, carbon source and concentration, nitrogen source, various pH levels were performed for the production of extracellular carboxymethyl cellulase and endoglucanase enzyme. The result exhibited, 15 % inoculums size, corncobs 2 % concentration, Urea and medium pH 7 at 30oC supported high yield of carboxymethyl cellulase (38.80 U/ml/min) and exoglucanase enzyme (10.94 U/ml/min) through a submerged fermentation (SmF). In future biotechnological applications in cellulase enzyme production attain a vital role to obtain high degradable yield.

scholarly journals Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
P. Saravanan ◽  
R. Muthuvelayudham ◽  
T. Viruthagiri
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Activity ◽  
Statistical Design ◽  
Cellulase Production ◽  
Positive Influence ◽  
Mango Peel ◽  
Soybean Cake ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Cake Flour

Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.

Plackett-Burman Design for Screening Culture Conditions in Cellulase Production by Penicillium decumbens and Enzyme Characterization

2012 ◽  
Vol 518-523 ◽  
pp. 5578-5585
Author(s):  
Nan Ban ◽  
Yu Jie Zhou ◽  
Yan Ping Ye ◽  
Lin Mei Dai ◽  
Alatangaole Damirn ◽  
...  
Keyword(s):  
Cellulase Activity ◽  
Enzyme Characterization ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Penicillium Decumbens ◽  
Initial Ph ◽  
Effects Of Ph ◽  
Burman Design ◽  
The Stability ◽  
Plackett Burman Design

Plackett-Burman design was employed for screening culture conditions for cellulase production by Penicillium decumbens in submerged fermentation. The results showed that wheat bran was the most significant factor influencing Filter Paper Activity (FPA) of the cellulase, followed by cellulose microcrystalline and initial pH, which could be further optimized for improving the cellulase activity. The effects of pH and temperature on FPA assay were investigated, and optimal FPA could be obtained at pH 4.5 and 60 °C. The stabilities of endo-glucanase (EG), exo-glucanase (CBH) and β-glucosidase (BG) were investigated and compared with that of FPA under different pH and temperature. The results indicated that CBH and FPA were more sensitive to pH and temperature than EG and BG and the stability of CBH was very similar to that of FPA under the conditions.

scholarly journals Optimization of fermentation condition for cellulase enzyme production from Bacillus sp.

2019 ◽  
Vol 2 (2) ◽  
pp. 19-24
Author(s):  
Maidul Islam ◽  
Palash Kumar Sarkar ◽  
A.K.M. Mohiuddin ◽  
Md. Suzauddula
Keyword(s):  
Enzyme Production ◽  
Production Condition ◽  
Fermentation Medium ◽  
Cellulase Production ◽  
Shake Flask Culture ◽  
Industrial Biotechnology ◽  
Bacillus Sp ◽  
Cellulase Enzyme ◽  
Optimization Of Fermentation

AbstractCellulase is an important enzyme in present-day of industrial biotechnology. The current study is concerned with the production and partial characterization of cellulase enzyme from Bacillus sp. The effect of various fermentation conditions for cellulase production through shake-flask culture was investigated. Maximum enzyme production was obtained after 24 hours of incubation in fermentation medium with pH 3.5 at 35°C under having agitation at 150 rpm while inoculums volume 1% was applied. Enzyme production was 1.91 times higher after optimizing the production condition as compared to the basal media. Cellulase characterization revealed that optimum activity was at pH 5.5 and 50°C for 50 minutes. About 68% of the activity retained after heating the crude enzyme solution at 50°C for 30 minutes. This nature makes cellulase a suitable candidate for current mainstream biomass conversions for sustainable agriculture and industrial processes.

Optimization of Fermentation Conditions for Cellulase Production by HDZK-BYTF620 (Aureobasidium pullulans) which is from the Gut of Termites

2014 ◽  
Vol 692 ◽  
pp. 167-171
Author(s):  
Ming Jing Sun ◽  
Li Qing Sun ◽  
Shan Shan Wang ◽  
Kai Zhao
Keyword(s):  
Aureobasidium Pullulans ◽  
Fermentation Medium ◽  
Cellulase Production ◽  
Fermentation Conditions ◽  
Steepest Ascent ◽  
Burman Design ◽  
Major Factors ◽  
Initial Medium ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

The components of a fermentation medium were optimized to produce cellulose from HDZK-BYTF620 (Aureobasidium pullulans) which is from the gut of termites and can produce cellulose under liquid state fermentation conditions by response surface methodology. By Plackett-Burman design screened out three major factors of strains HDZK-BYTF620 which affected production of the activity cellulose, immediately, CaCl2, MgSO4and CMC-Na. The fermentation conditions were optimized with Box-Behnken experiment and the largest regional of the activity of cellulase was approach with the steepest ascent experiment. The optimum composition of medium addition of CaCl2, MgSO4and CMC-Na was 0.77 g/L, 0.41 g/L and 21.88 g/L, respectively. Under the optimum fermentation medium conditions, the activity of cellulase was (598.00±2.56)×10-3IU/mL, increased 5.0 % to that of using initial medium.

Cellulase Production and Characterization from Thermomucor indicae-seudaticae

2021 ◽  
Vol 16 (11) ◽  
pp. 69-77
Author(s):  
Ashima Kathuria ◽  
Swati Allen
Keyword(s):  
Enzyme Activity ◽  
Enzyme Production ◽  
Incubation Temperature ◽  
Paper Industry ◽  
Cellulose Hydrolysis ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Biofuel Production ◽  
Beverage Industry ◽  
Triton X

Cellulases are one of the important groups of industrial hydrolases which hydrolyze lignocellulosics into glucose, hence find numerous applications in the food, feed and beverage industry, textile, pulp and paper industry and biofuel production. With a view of industrial importance of cellulases, this study aimed to screen the cellulolytic potential of fungus Thermomucor indicae-seudaticae sp. nov. The zone of cellulose hydrolysis producing around the growth of fungus confirmed its cellulase-producing ability. Further, the enzyme production was carried out under liquid fermentation and various culture conditions were optimized. Among the physical conditions, maximum enzyme production was obtained at pH 7.0, incubation temperature of 50°C and incubation period of 7 days. Corn cob as carbon source followed by rice straw enhanced the enzyme production. Yeast extract was found to be the best nitrogen source. The crude enzyme exhibited highest enzyme activity at pH 7.0 and temperature 45oC. Among the various additives tested, some metal ions (Na+, K+, Ca+2 and Mg+2) stimulated the enzyme activity while Hg+2 strongly inhibited the enzyme. Among the detergents, SDS had stimulatory effect while Triton X-100 was found inhibitory for cellulase.

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