Plackett-Burman Design for Screening Culture Conditions in Cellulase Production by Penicillium decumbens and Enzyme Characterization

2012 ◽  
Vol 518-523 ◽  
pp. 5578-5585
Author(s):  
Nan Ban ◽  
Yu Jie Zhou ◽  
Yan Ping Ye ◽  
Lin Mei Dai ◽  
Alatangaole Damirn ◽  
...  
Keyword(s):  
Cellulase Activity ◽  
Enzyme Characterization ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Penicillium Decumbens ◽  
Initial Ph ◽  
Effects Of Ph ◽  
Burman Design ◽  
The Stability ◽  
Plackett Burman Design

Plackett-Burman design was employed for screening culture conditions for cellulase production by Penicillium decumbens in submerged fermentation. The results showed that wheat bran was the most significant factor influencing Filter Paper Activity (FPA) of the cellulase, followed by cellulose microcrystalline and initial pH, which could be further optimized for improving the cellulase activity. The effects of pH and temperature on FPA assay were investigated, and optimal FPA could be obtained at pH 4.5 and 60 °C. The stabilities of endo-glucanase (EG), exo-glucanase (CBH) and β-glucosidase (BG) were investigated and compared with that of FPA under different pH and temperature. The results indicated that CBH and FPA were more sensitive to pH and temperature than EG and BG and the stability of CBH was very similar to that of FPA under the conditions.

Optimization of Culture Conditions for Cold-Active Cellulase Production by Penicillium cordubense D28 Using Response Surface Methodology

2011 ◽  
Vol 183-185 ◽  
pp. 994-998
Author(s):  
Shuo Dong ◽  
Nai Yu Chi ◽  
Qing Fang Zhang
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Batch Reactors ◽  
Corn Meal ◽  
Cold Active ◽  
Initial Ph ◽  
Cost Efficient

The design of an optimum and cost-efficient medium for production of cold-active cellulase by Penicillium cordubense D28 was attempted by using response surface methodology (RSM). Based on the Plackett–Burman design, corn meal, (NH4)2SO4 and branc were selected as the most critical nutrient. Subsequently, they were investigated by the Box-Behnken design. Results showed that the maximum cold-active cellulase activity of 110.4U/mL was predicted when the concentration of corn meal, (NH4)2SO4 and branc were 21.97 g/L, 2.39 g/L and 14.99 g/L, respectively. The results were further verified by triplicate experiments. The batch reactors were operated under an optimized condition of the respective corn meal, (NH4)2SO4 and branc concentration of 22 g/L , 2.4 g/L and 15 g/L , the initial pH of 6.0 and experimental temperature of 20 ± 1°C. Without further pH adjustment, the maximum cold-active cellulase activity of 109.8 U/mL was obtained based on the optimized medium with further verified the practicability of this optimum strategy.

scholarly journals Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
P. Saravanan ◽  
R. Muthuvelayudham ◽  
T. Viruthagiri
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Activity ◽  
Statistical Design ◽  
Cellulase Production ◽  
Positive Influence ◽  
Mango Peel ◽  
Soybean Cake ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Cake Flour

Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.

scholarly journals Optimization of Acid Protease Production byAspergillus nigerI1 on Shrimp Peptone Using Statistical Experimental Design

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Rayda Siala ◽  
Fakher Frikha ◽  
Samiha Mhamdi ◽  
Moncef Nasri ◽  
Alya Sellami Kamoun
Keyword(s):  
Culture Conditions ◽  
Medium Composition ◽  
Acid Protease ◽  
Protease Production ◽  
Statistical Experimental Design ◽  
Initial Ph ◽  
Burman Design ◽  
The Cost ◽  
Optimized Conditions ◽  
Plackett Burman Design

Medium composition and culture conditions for the acid protease production byAspergillus nigerI1 were optimized by response surface methodology (RSM). A significant influence of temperature, KH2PO4, and initial pH on the protease production was evaluated by Plackett-Burman design (PBD). These factors were further optimized using Box-Behnken design and RSM. Under the proposed optimized conditions, the experimental protease production (183.13 U mL−1) closely matched the yield predicted by the statistical model (172.57 U mL−1) withR2=0.914. Compared with the initial M1 medium on which protease production was 43.13 U mL−1, a successful and significant improvement by 4.25 folds was achieved in the optimized medium containing (g/L): hulled grain of wheat (HGW) 5.0; KH2PO41.0; NaCl 0.3; MgSO4(7H2O) 0.5; CaCl2(7H2O) 0.4; ZnSO40.1; Na2HPO41.6; shrimp peptone (SP) 1.0. The pH was adjusted at 5 and the temperature at30°C. More interestingly, the optimization was accomplished using two cheap and local fermentation substrates, HGW and SP, which may result in a significant reduction in the cost of medium constituents.

scholarly journals BNC Biosynthesis with Increased Productivity in a Newly Designed Surface Air-Flow Bioreactor

2020 ◽  
Vol 10 (11) ◽  
pp. 3850
Author(s):  
Izabela Cielecka ◽  
Małgorzata Ryngajłło ◽  
Stanisław Bielecki
Keyword(s):  
Mechanical Properties ◽  
Air Flow ◽  
Culture Conditions ◽  
Production Costs ◽  
High Yield ◽  
Flow Ratio ◽  
Culture Time ◽  
Wide Range ◽  
Burman Design ◽  
Plackett Burman Design

The application of bacterial cellulose (BNC) could be widely expanded if the production costs were reduced. This study aims to determine factors simultaneously affecting the yield and tensile strength of BNC in a newly designed surface air-flow bioreactor (SAF). For this purpose, a two-stage study was done. Firstly, the most important factors for high yield were determined based on the Plackett–Burman Design. Secondly, impact of the chosen variables on both responses was assessed in a wide range of factor values. The greatest influence on the yield and mechanical strength was proved for such factors as air-flow ratio, glucose concentration, and culture time. The productivity in a SAF bioreactor with controlled air-flow ratio was enhanced by 65%. In terms of mechanical properties, the stress of BNC membranes varied from 0.8 to 6.39 MPa depending on the culture conditions. The results of the performed tests make a useful basis for future optimizations.

Optimization of Culture Parameters for Expression of Recombinant Human Basic Fibroblast Growth Factor in Pichia pastoris Using Response Surface Methodology Combining with Plackett-Burman Design

2013 ◽  
Vol 647 ◽  
pp. 456-461 ◽  
Author(s):  
Rui Jiang ◽  
Li Wei Sun ◽  
Yu Zhao ◽  
Kai Feng ◽  
Shou Yan Chen
Keyword(s):  
Response Surface Methodology ◽  
Growth Factor ◽  
Pichia Pastoris ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Fermentation Time ◽  
Initial Ph ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Significant Factors

In this study, we used plackett-burman design (PBD) and response surface methodology (RSM) methods to optimize media and culture conditions for expression of recombinant human basic fibroblast growth factor (rhbFGF) in Pichia pastoris in order to effectively enhance the production of rhbFGF. PBD was used to screen the most significant factors from nine important factors, which were fermentation time, methanol, temperature, initial pH, OD600 for inducement, concentrated volume, YNB peptone, and casein. Then RSM was further used to optimize the significant parameters. Our results had shown that three significant factors selected by PBD were temperature, fermentation time, and methanol. Base on the 3-factor-3-level central composite design (CCD) of RSM, the final optimized conditions were fermentation time 126.7h, methanol 1.3%, temperature 21.3°C, initial pH 6.0±0.1, OD600 for inducement 26, concentrated volume 100 ml, YNB 13.4%, peptone 4%, and casein 0.1%. The maximum production of rhbFGF was 302.4mg/L, which was 2 times higher than the production of rhbFGF reported in the previous studies. Those results provided the foundation for further researches on higher density cultivation condition of rhbFGF explored in the fermenter and accelerate the industrialization development of rhbFGF.

scholarly journals Optimization of Large-Scale Culture Conditions for the Production of Cordycepin withCordyceps militarisby Liquid Static Culture

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Chao Kang ◽  
Ting-Chi Wen ◽  
Ji-Chuan Kang ◽  
Ze-Bing Meng ◽  
Guang-Rong Li ◽  
...  
Keyword(s):  
Large Scale ◽  
Total Content ◽  
Culture Conditions ◽  
Key Factors ◽  
Working Volume ◽  
Large Scale Culture ◽  
Factor Design ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

Cordycepin is one of the most important bioactive compounds produced by species ofCordyceps sensu lato, but it is hard to produce large amounts of this substance in industrial production. In this work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.

scholarly journals PRODUCTION OF ALPHA AMYLASE AND CELLULASE FROM SOLID STATE CULTURE OF ASPERGILLUS OCHRACEUS: A FEASIBILITY ANALYSIS

2021 ◽  
Vol 4 (3) ◽  
pp. 1-3
Author(s):  
Sohail Khan ◽  
Ashwani Mathur
Keyword(s):  
Solid State ◽  
Cellulase Activity ◽  
Cost Effective ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Alpha Amylase ◽  
Aspergillus Ochraceus ◽  
Trichoderma Longibrachiatum ◽  
State Culture ◽  
Cost Effective Alternative

The growing demand and application of industrially important enzyme necessitate the need to explore new sources with diverse enzymes ranging in their specificity and activities. Enzymes are safe alternatives to chemical synthesis due to minimum side effect and ease of manufacturing. Solid state fermentation (SSF) is a cost-effective alternative to submerged fermentation with agro-residues or waste, often being used as substrate for growing diverse organisms for production of metabolites. Current study is one of the scarce report on exploring alpha amylase and cellulase production ability Aspergillus ochraceus (MTCC 1877) using wheat bran as substrate at relative humidity of 90% and at 30 ºC, for 7 days. Result showed the potential of Aspergillus ochraceus (MTCC 1877), as potential source of the two enzymes. Results revealed comparatively higher alpha amylase activity in the SSF extract of Aspergillus ochraceus (MTCC 1877) in comparison to Trichoderma longibrachiatum (ITCC 7839). On the contrary, comparatively higher cellulase activity was observed in the SSF extract of Trichoderma longibrachiatum (ITCC 7839). The results showed the potential of Aspergillus ochraceus (MTCC 1877) as a source of the two enzymes. Variation in enzymes activity may be attributed to the experimental culture conditions and may be further optimized to enhance the enzymes yield.

scholarly journals Potential of Aspergilus Niger MT809753 for Bio-treatment Paper Industry Wastewater After Screening Effective Factors on Production of Cellulase Enzyme by Plackett-burman Design

2021 ◽  
Author(s):  
Marwa El-Sesy ◽  
Amira M Aly
Keyword(s):  
Rice Straw ◽  
Enzyme Production ◽  
Paper Industry ◽  
Cellulase Production ◽  
Barley Straw ◽  
Cellulase Enzyme ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Aspergilus Niger ◽  
Industry Wastewater

Abstract A microorganism capable of degrading cellulose present in rice straw was isolated from wastewater samples and identified as Aspergilus niger MT809753 by 18S rDNA. In the present study various cheap agronomic cellulosic wastes as (cotton seed husks, barley straw, rice straw and maize straw) were utilized as crude inducers for the cellulase enzyme production and represent the carbon source for isolates where cellulose activity was measured by (DNS) method. The highest cellulases enzyme production was obtained by fungal isolate Aspergilus niger MT809753 within 24 hours (0.532 IU/ml) using rice straw. Plackett-Burman design was used as conventional method for statistically screening of different variables. Nine variables of the production process were selected. The results illustrate those seven variables, namely as (inoculum size, substrate concentration, incubation temperature, pH, shaking conditions, and incubation time and peptone concentration) had influence with high confidence levels, while the remaining two variables did not show a significant effect on cellulase production. After using response optimization the experiment was performed and the obtained cellulase production was 1.08 IU/ml. A bench scale study was performed to examine paper industry wastewater treatment efficiency by Aspergillus Niger MT809753. Results reveal that organisms have proved their bioremediation potency in treatment of paper industry effluent. The importance of the research stems from the fact that it sheds light on the role of some fungi in the production of the cellulase enzyme. So our goal is to obtain local isolates from fungi having a high ability to produce the cellulase enzyme, as well as developing an effective treatment processes to get rid of environmental cellulosic pollution and utilization of cellulosic wastes as cheap carbon sources.

scholarly journals CHARACTERIZATION OF CRUDE CELLULASE OF SEAGRASS ENDOPHYTIC FUNGUS

2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Yulia Oktavia ◽  
Aulia Andhikawati ◽  
Tati Nurhayati ◽  
Kustiariyah Tarman
Keyword(s):  
Endophytic Fungus ◽  
Cellulase Activity ◽  
Enzyme Characterization ◽  
Cellulase Production ◽  
Fungal Culture ◽  
Endoglucanase Activity ◽  
Maximum Reaction Rate ◽  
Glucosidase Activity ◽  
Maximum Reaction

<p>In this study, cellulase was produced by endophytic fungus isolated from seagrass. Substrate used for incubating the fungus was the waste of agar industry. The objectives of this study were to optimize cellulase production and to characterize the highest activity of fungal crude cellulase. In our previous study, the EN isolate (isolated from <span style="text-decoration: underline;">Enhalus</span> <span style="text-decoration: underline;">sp</span>.) showed the highest cellulolytic index. Therefore, in this research we focused on cellulase activity of the isolate. Cellulase activity was determined based on endoglucanase activity, total cellulase activity, and β-glucosidase activity. The highest activity was then used to determine cellulase activity in enzyme characterization. The fungus was cultured in different concentration of agar extraction algal wastes. The fungal culture was incubated for 3-21 days with 120 rpm orbital shaker. The results showed that endoglucanase activity was 0.019-0.031 U/mL, total cellulase activity was 0.007-0.013 U/mL, and β-glucosidase activity was 0.00012-0.00361 U/mL. The highest endoglucanase and total cellulase activity were obtained from the fungal culture after 9 days incubation, β-glucosidase was obtained from the fungal culture after 15 days incubation with 1.5% of algal waste as substrate. The optimum pH and temperature were determined as 4 and 60 <sup>o</sup>C, maximum reaction rate (V<sub>max</sub>) and Michaelis-Menten constant (K<sub>m</sub>) for endoglucanase activity was determined as 0.044 U/mL and 0.103% respectively.</p> <p>Keywords: algal waste, cellulase, endophytes, enzymes, seagrass</p>

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