scholarly journals Isolation and Identification of Lactobacillus plantarum C010 and Growth Kinetics of its Batch Fermentation

2021 ◽  
Author(s):  
Jinyue Dai ◽  
Limin fang ◽  
Manmin Zhang ◽  
Huaili Deng ◽  
Xin Cheng ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Batch Fermentation ◽  
Proteinase K ◽  
Isolation And Identification ◽  
16S Rdna Gene ◽  
Logarithmic Growth Phase ◽  
Gene Sequence Analysis ◽  
Kinetics Of ◽  
Logarithmic Growth ◽  
Chilled Pork

Abstract Chilled pork is pursuit by people due to its delicious and delicate taste, but it is still susceptible to microbial contamination even under refrigerated conditions. Consequently, to explore microbial preservatives for chilled pork, in this study, the specific spoilage organism Pseudomonas koreensis PS1 from spoiled chilled pork as the indicator was used to isolate the bacteriocin-producing lactic acid bacteria from the soils and fresh cow dungs. Among six bacteriocin-producing bacteria from 182 isolates, the strain C010 with higher-yielding, broad-spectrum, subculture stability and protease (pepsin, trypsin and proteinase K) sensitive was selected and identified as Lactobacillus plantarum based on morphological, biochemical and 16S rDNA gene sequence analysis. Simultaneously, the crude bacteriocin of L. plantarum C010 was stable under high temperature and ultraviolet conditions. The kinetics of bacterial growth and bacteriocin production of L. plantarum C010 were analyzed in batch fermentation. Bacteriocin was produced throughout the logarithmic growth phase and the Leudeking-piret model could characterize the synthesis of bacteriocin well. This present study indicates that bacteriocin-producing L. plantarum C010 has promising potentials to control the specific spoilage organism and can be used as the bio-preservative in food.

scholarly journals Isolation and Identification of Bacteriocin-Producing Lactic Acid Bacteria from Urutan, Balinese Traditional Fermented sausage

2021 ◽  
Vol 8 (1) ◽  
pp. 81
Author(s):  
Ni Made Sri Dwijastuti ◽  
I Nengah Sujaya ◽  
Ni Nengah Dwi Fatmawati
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Enterococcus Faecalis ◽  
Indicator Bacteria ◽  
Isolation And Identification ◽  
16S Rdna Gene ◽  
Fermented Sausage ◽  
Inhibition Zones

This study aimed to isolate and identify lactic acid bacteria (LAB) that could produce bacteriocins from urutan. Urutan is a Balinese sausage that is traditionally produced and naturally fermented. Urutans used in this study were obtained from several traders in the Baturiti, Mengwi, and Tegallalang region, Bali. More than 300 LAB colonies were isolated and underwent inhibition test against the growth of the indicator bacteria. Crude bacteriocins from two isolates, namely J2 and J6, showed inhibition zones against the growth of Lactobacillus plantarum E12.1 and Escherichia coli ATCC 8739. The J6 isolate also inhibited the growth of Enterococcus faecalis ATCC 29212. Both J2 and J6 were gram-positive, bacilli shaped, and showed negative catalase test. Based on the results of the 16S rDNA gene amplification, the two isolates were identified as Lactobacillus plantarum. Keywords: Lactic Acid Bacteria, Bacteriocin, Lactobacillus plantarum, Urutan

scholarly journals Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces

2007 ◽  
Vol 50 (2) ◽  
pp. 249-258 ◽  
Author(s):  
Alessandra Einsfeld Ferreira ◽  
Natália Canal ◽  
Daiana Morales ◽  
Daiane Bopp Fuentefria ◽  
Gertrudes Corção
Keyword(s):  
Molecular Weight ◽  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Storage Test ◽  
Logarithmic Growth Phase ◽  
Primary Metabolite ◽  
Enterococcus Mundtii ◽  
Kinetics Of ◽  
Logarithmic Growth

The aim of this study was to characterize bacteriocins produced by 70 strains of Enterococcus mundtii.Four strains exhibited antibiotic activity towards Listeria innocua, L. monocytogenes, Lactobacillus plantarum, and Salmonella Enteritidis. They remained active under temperatures of up to 121ºC for 20 min, and under pH treatments that varied from 2.0 to 10.0. Antimicrobial activity was maintained during the storage test for 60 days under freezing. The kinetics of production revealed the peak activity of 1600 AU /mL during the logarithmic growth phase and the molecular weight found was approximately 3.0 kDa. The characterization of the products with antimicrobial activity indicated their proteic nature, presenting a typical kinetics of primary metabolite and a molecular weight similar to many purified enterocins.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

scholarly journals Isolation and identification of bacteriocinogenic strain of Lactobacillus plantarum with potential beneficial properties from donkey milk

2013 ◽  
Vol 114 (6) ◽  
pp. 1793-1809 ◽  
Author(s):  
A. Murua ◽  
S.D. Todorov ◽  
A.D.S. Vieira ◽  
R.C.R. Martinez ◽  
A. Cencič ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Donkey Milk ◽  
Isolation And Identification

Phenyllactic acid production by fed-batch fermentation of Lactobacillus plantarum CECT-221

2010 ◽  
Vol 150 ◽  
pp. 320-320 ◽  
Author(s):  
Noelia Rodríguez ◽  
Jose Manuel Salgado ◽  
Belén Max ◽  
Sandra Cortés ◽  
Jose Manuel Domínguez
Keyword(s):  
Lactobacillus Plantarum ◽  
Acid Production ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Phenyllactic Acid ◽  
Fed Batch Fermentation

Degradation of zearalenone in swine feed and feed ingredients by Bacillus subtilis ANSB01G

2014 ◽  
Vol 7 (2) ◽  
pp. 143-151 ◽  
Author(s):  
Y.P. Lei ◽  
L.H. Zhao ◽  
Q.G. Ma ◽  
J.Y. Zhang ◽  
T. Zhou ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Culture Supernatant ◽  
Antimicrobial Activities ◽  
Proteinase K ◽  
Rrna Gene ◽  
Distillers Grains With Solubles ◽  
Dried Distillers Grains ◽  
Cell Extracts ◽  
Gene Sequence Analysis ◽  
And Staphylococcus Aureus

Zearalenone (ZEA) and its derivatives are mycotoxins that can cause oestrogenic effects and impair the reproductive physiology of animals, especially in female swine. Strategies to reduce or eliminate ZEA contamination in foods and feeds are very much needed. Among 36 bacterial isolates obtained from a variety of animal intestinal chyme, mouldy foods and feeds, soils, etc., five isolates demonstrated the ability to reduce more than 50% of ZEA in a liquid medium; ANSB01G isolate taken from normal broiler intestinal chyme reduced ZEA the most, by 88.65%. Using physiological, biochemical, and 16S rRNA gene sequence analysis methods, the ANSB01G isolate was identified as Bacillus subtilis. Under simulated intestinal tract conditions, the ANSB01G B. subtilis isolate degraded 84.58, 66.34 and 83.04% of ZEA in naturally contaminated maize, dried distillers’ grains with solubles, and swine complete feed, respectively. The highest degradation of ZEA occurred when the mycotoxin was co-incubated with the whole bacterial culture, resulting in a reduction of 88.65%, followed by 75.60% using culture supernatant, 26.11% using cell extracts, and 15.06% using viable cells. Treatments consisting of both heating and addition of proteinase K significantly reduced the rate of ZEA degradation in the culture supernatant, indicating that the ZEA degradation might be enzymatic. B. subtilis ANSB01G displayed resistance to simulated gastrointestinal tract environments and antimicrobial activities against several common bacterial pathogens, including Escherichia coli, Salmonella typhimurium and Staphylococcus aureus. These properties of B. subtilis ANSB01G suggest the possibility of its potential to effectively degrade ZEA in feed and to develop functional feed products for livestock industries.

scholarly journals Nocardia farcinica - a significant cause of mastitis in goats in Sudan : short communication

2004 ◽  
Vol 75 (3) ◽  
Author(s):  
L.A. Maldonado ◽  
M.E. Hamid ◽  
O.A. Gamal El Din ◽  
M. Goodfellow
Keyword(s):  
16S Rdna ◽  
Short Communication ◽  
Gene Sequencing ◽  
Isolation And Identification ◽  
16S Rdna Gene ◽  
Identification Methods ◽  
Milk Samples ◽  
Nocardia Farcinica ◽  
Mastitic Milk ◽  
16S Rdna Gene Sequencing

Fifteen of 100 mastitic milk samples from goats suffering from mastitis were tentatively identified as members of the genus Nocardia on the basis of selected phenotypic and chemotaxonomic characteristics. Six of the 15 strains were confirmed as Nocardia farcinica by 16S rDNA gene sequencing and subsequent aligning with relevant actinomycetes found in electronic databases and 2 by other identification criteria. N. farcinica is a serious cause of mastitis with a significant prevalence (15%) among the examined goats. Efforts are needed to optimise and simplify isolation and identification methods.

Regeneration of protoplasts in Saccharomyces

1972 ◽  
Vol 18 (11) ◽  
pp. 1773-1775 ◽  
Author(s):  
M. M. Shahin
Keyword(s):  
Growth Phase ◽  
Transition Phase ◽  
Protoplast Formation ◽  
Logarithmic Growth Phase ◽  
High Degree ◽  
Logarithmic Growth

Protoplasts prepared from cells of different stages within the logarithmic growth phase and from transition phase showed different degrees of colony-forming ability. The cells yielding higher frequency of protoplast formation also gave protoplast with a high degree of colony-forming ability.

scholarly journals In vitro and In Silico Approach For Characterization of Antimicrobial Peptide From Probiotics Against Staphylococcus Aureus and Escherichia Coli

2021 ◽  
Author(s):  
Amrutha Bindu ◽  
Lakshmi Devi
Keyword(s):  
Amino Acid ◽  
Antimicrobial Peptide ◽  
Lactobacillus Plantarum ◽  
Exchange Chromatography ◽  
Amino Acid Sequences ◽  
Proteinase K ◽  
Kinetic Assay ◽  
Wide Range

Abstract The focus of present study was to characterize antimicrobial peptide produced by probiotic cultures, Enterococcus durans DB-1aa (MCC4243), Lactobacillus plantarum Cu2-PM7 (MCC4246) and Lactobacillus fermentum Cu3-PM8 (MCC4233) against Staphylococus aureus and E. coli. The growth kinetic assay revealed 24 h of incubation to be optimum for bacteriocin production. The partially purified compound after ion-exchange chromatography was found to be thermoresistant and stable under wide range of pH. The compound was sensitive to proteinase-K, but resistant to trypsin, a-amylase and lipase. The apparent molecular weight of bacteriocin from MCC4243 and MCC4246 was found to be 3.5 KDa. Translated partial amino acid sequence of plnA gene in MCC4246 displayed 48 amino acid sequences showing 100% similarity with plantaricin A of Lactobacillus plantarum (WP_0036419). The sequence revealed 7 β sheets, 6 α sheets, 6 predicted coils and 9 predicted turns. The functions on cytoplasm show 10.82 isoelectric point and 48.6% hydrophobicity. The molecular approach of using Geneious Prime software and protein prediction data base for characterization of bacteriocin is novel and predicts “KSSAYSLQMGATAIKQVKKLFKKWGW” as peptide responsible for antimicrobial activity. The study provides information about broad spectrum bacteriocin in native probiotic culture and paves a way towards its application in functional foods as biopreservative agents.

scholarly journals A and alpha supernatant pretreatment of Saccharomyces cerevisiae cells affects both the kinetics and efficiency of mating.

1982 ◽  
Vol 2 (8) ◽  
pp. 897-903 ◽  
Author(s):  
E P Sena
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Fusion ◽  
Large Scale ◽  
Culture Supernatant ◽  
Treatment Procedure ◽  
Logarithmic Growth Phase ◽  
Future Studies ◽  
Mating Efficiency ◽  
Potential Applications ◽  
Logarithmic Growth

The effects of culture supernatant treatment on subsequent matings between pretreated a and alpha Saccharomyces cerevisiae cells were studied. For each experiment, pairs of a and alpha [rho+] or [rho- rho0] cells in the logarithmic growth phase in defined minimal medium were pretreated for a total of 15 min (by exchanging their cell-free supernatants or by mixing samples of a and alpha cell cultures) and then mated in defined minimal (YNB) or enriched (YEP) liquid medium. All pretreated cells, regardless of treatment procedure, initiated cell fusion 15 to 35 min faster than did their nontreated counterparts. In all cases, pretreated cells mated 8 to 20% more efficiently than did nonpretreated ones. Regardless of the strains, the hierarchy of mating efficiency was always treated YEP greater than untreated YEP greater than treated YNB greater than untreated YNB. The cell fusion kinetics in alpha [rho+] X a [rho-] crosses were most affected by pretreatment (delta 30 to 35 min), whereas [rho+] X [rho+] crosses were least affected (delta 15 min). These results are discussed in relation to the functions known for a and alpha pheromones. The successful pretreatment regimes were used to design new rapid and efficient techniques for mating YNB-grown log-phase cells in either YNB or YEP liquid media. These techniques can be used for small- or large-scale mating, and because of their inherent media flexibility, they have many potential applications to future studies on mating-specific or intrazygotic phenomena.

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