scholarly journals Leaf Transcriptome Analysis of Medicago Ruthenica, Revealing Its Response and Adaptive Strategy to Drought Stress and Rehydration

2020 ◽  
Author(s):  
Rina Wu ◽  
Bo Xu ◽  
Fengling Shi
Keyword(s):  
Drought Stress ◽  
Resistance Genes ◽  
Stress Resistance ◽  
Sucrose Metabolism ◽  
High Yield ◽  
Severe Drought ◽  
Proline Metabolism ◽  
Adaptation Mechanism ◽  
Medicago Ruthenica ◽  
Antenna Proteins

Abstract Background: Recently, drought stress has brought tremendous loss on the production of agriculture and animal husbandry. In realistic production, plants are often in cyclic wet-dry environment. Therefore, the factors that affect the final yield of plants in adversity including the resistance and tolerance to drought and the ability of plants to resume from the previous damage after rehydration. So it’s necessary for us to study the response and adaptive strategies of plants to drought and rehydration. Generally, the yield of herbage with strong resistance is relatively low. However, Medicago ruthenica(L.)cv.Zhilixing has the advantages of strong resistance and high yield concurrently. This made it can be used for raising livestock, natural grassland improvement, as a good parent for breeding and a new and high quality resource of stress resistance genes. Now, there are still many problems need to be solved when compared with other important legume forages. Therefore, we analyzed the changes of Medicago ruthenica(L.)cv.Zhilixing on transcription level under drought stress and rehydration, explored its phased response strategies.Results: We obtained 191 DEGs in drought stress, and the three treatments has 43 DEGs in common. Galactose metabolism, Starch and sucrose metabolism, Arginine and proline metabolism, TCA cycle, Photosynthesis-antenna proteins, were involved in the adaptation of Medicago ruthenica to 9 days of drought stress. The regulation of Arginine and proline metabolism, Cysteine and methionine metabolism, Photosynthesis-antenna proteins, Ascorbate and aldarate metabolism were conducive to the resistance of Medicago ruthenica to severe drought stress. The regulation of Starch and sucrose metabolism, Flavonoid biosynthesis, Valine, leucine and isoleucine degradation, Circadian rhythm-plant was beneficial to the post drought recovery of Medicago ruthenica.Conclusions: We preliminarily analyzed the adaptation mechanism of the plant under different drought and rehydration conditions. Medicago ruthenica(L.)cv.Zhilixing adopts different strategies to adapt to different degrees of drought stress and rehydration. The research discovered the genes that can be used as candidate genes to improve stress resistance and drought adaptability of plants. Our transcriptome data dramatically enriches the resources of stress resistance genes. It can provide theoretical support for further adaptation mechanism research of the plant under different drought and rehydration conditions.

scholarly journals HEAT AND DROUGHT STRESS EFFECT IN WHEAT GENOTYPES: A REVIEW

2021 ◽  
Vol 1 (2) ◽  
pp. 77-79
Author(s):  
Sandesh Paudel ◽  
Netra Prasad Pokharel ◽  
Susmita Adhikari ◽  
Sarah Poudel
Keyword(s):  
Heat Stress ◽  
Drought Stress ◽  
Harmful Effect ◽  
Winter Season ◽  
Triticum Aestivum L ◽  
High Yield ◽  
Severe Drought ◽  
Stress Effect ◽  
Cereal Crop ◽  
Drought And Heat Stress

Bread wheat (Triticum aestivum L.) belonging to family Poaceae is the most important cereal crop as it contributes major portion to the world food for the world’s population. Similarly, it is the third most cultivated cereal crop in Nepal in terms of production and area. Wheat is a winter season crop which is usually grown within a temperature range of 15-250C in cold and dry weather. However frequent irrigations are crucial for proper growth of the plant, high yield and high quality of the grain. The annual productivity of wheat has been reported to be 2.49 tons per hectare. Water is found to be one of the most important factors in wheat production and by far not a single water stress tolerant variety has been introduced thus water management is necessary. In Nepal around 35% of the total wheat is cultivated under rainfed condition annually and in Terai this is around 19%. This cultivated area faces a severe drought stress during growing stage and heat stress during anthesis stage. Various studies have suggested that the combined impacts of drought and heat stress had a significant harmful effect on wheat than individual stresses (Stress and Review, 2017). Under drought stress days to anthesis and days to maturity were reduced by 10% and 14% while under heat stress these were reduced by 16% and 20% respectively. Combined effect of drought and heat stress caused reduction in DTA by 25% DTH by and 31%.

scholarly journals Effects of Salt-Drought Stress on Growth and Physiobiochemical Characteristics ofTamarix chinensisSeedlings

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Junhua Liu ◽  
Jiangbao Xia ◽  
Yanming Fang ◽  
Tian Li ◽  
Jingtao Liu
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Oxidation Resistance ◽  
Stress Resistance ◽  
Salt Resistance ◽  
Severe Drought ◽  
Tamarix Chinensis ◽  
Biochemical Characteristics ◽  
Factors Influencing ◽  
Salt And Drought Stresses

The present study was designed to clarify the effects of salinity and water intercross stresses on the growth and physiobiochemical characteristics ofTamarix chinensisseedlings by pots culture under the artificial simulated conditions. The growth, activities of SOD, POD, and contents of MDA and osmotic adjusting substances of three years old seedlings ofT. chinensiswere studied under different salt-drought intercross stress. Results showed that the influence of salt stress on growth was greater than drought stress, the oxidation resistance of SOD and POD weakened gradually with salt and drought stresses intensified, and the content of MDA was higher under severe drought and mild and moderate salt stresses. The proline contents increased with the stress intensified but only significantly higher than control under the intercross stresses of severe salt-severe drought. It implied thatT. chinensiscould improve its stress resistance by adjusted self-growth and physiobiochemical characteristics, and the intercross compatibility ofT. chinensisto salt and drought stresses can enhance the salt resistance under appropriate drought stress, but the dominant factors influencing the physiological biochemical characteristics ofT. chinensiswere various with the changing of salt-drought intercross stresses gradients.

scholarly journals Role of Suillus placidus in Improving the Drought Tolerance of Masson Pine (Pinus massoniana Lamb.) Seedlings

Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 332
Author(s):  
Min Li ◽  
Haoyun Wang ◽  
Xizhou Zhao ◽  
Zhongke Lu ◽  
Xueguang Sun ◽  
...  
Keyword(s):  
Drought Stress ◽  
Defense Mechanism ◽  
Southern China ◽  
Dry Weight ◽  
Pinus Massoniana ◽  
Photosynthetic Performance ◽  
Ectomycorrhizal Fungus ◽  
Severe Drought ◽  
Masson Pine ◽  
Pine Seedlings

Masson pine is an important afforestation species in southern China, where seasonal drought is common. The present study focused on the effects of Suillus placidus, an ectomycorrhizal fungus, inoculation on the growth and physiological and biochemical performance of masson pine seedlings under four different watering treatments (well-watered, mild drought, moderate drought, and severe drought) to evaluate the symbiotic relationship between S. placidus and masson pine seedlings. Ectomycorrhizal-inoculated (ECM) and non-inoculated (NM) seedlings were grown in pots and maintained for 60 days using the weighing method. Results showed that seedlings’ growth, dry weight, RWC, chlorophyll content, PSII efficiency, and photosynthesis decreased as drought stress intensified in both ECM and NM plants. This suggests that drought stress significantly limits the growth and photosynthetic performance of masson pine seedlings. Nevertheless, increased An/gs and proline contents in both NM and ECM prevented oxidative damage caused by drought stress. In addition, increased peroxidase (POD) activity is an essential defense mechanism of ECM seedling under drought stress. Compared with NM, ECM seedlings showed faster growth, higher RWC, and photosynthetic performance, and lower lipid peroxidation in cell membranes under drought stress, as indicated by higher POD activity and lower proline and malondialdehyde (MDA). Our experiment found that S. placidus inoculation can enhance the drought resistance of masson pine seedlings by increasing antioxidant enzyme activity, water use efficiency, and proline content, thereby enhancing growth under water-deficiency conditions. S. placidus can be used to cultivate high-quality seedlings and improve their survival in regions that experience seasonal droughts.

scholarly journals Spermine: Its Emerging Role in Regulating Drought Stress Responses in Plants

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 261
Author(s):  
Md. Mahadi Hasan ◽  
Milan Skalicky ◽  
Mohammad Shah Jahan ◽  
Md. Nazmul Hossain ◽  
Zunaira Anwar ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Defense Mechanisms ◽  
Antioxidant Defense ◽  
Severe Drought ◽  
New Information ◽  
Effects Of Drought

In recent years, research on spermine (Spm) has turned up a lot of new information about this essential polyamine, especially as it is able to counteract damage from abiotic stresses. Spm has been shown to protect plants from a variety of environmental insults, but whether it can prevent the adverse effects of drought has not yet been reported. Drought stress increases endogenous Spm in plants and exogenous application of Spm improves the plants’ ability to tolerate drought stress. Spm’s role in enhancing antioxidant defense mechanisms, glyoxalase systems, methylglyoxal (MG) detoxification, and creating tolerance for drought-induced oxidative stress is well documented in plants. However, the influences of enzyme activity and osmoregulation on Spm biosynthesis and metabolism are variable. Spm interacts with other molecules like nitric oxide (NO) and phytohormones such as abscisic acid, salicylic acid, brassinosteroids, and ethylene, to coordinate the reactions necessary for developing drought tolerance. This review focuses on the role of Spm in plants under severe drought stress. We have proposed models to explain how Spm interacts with existing defense mechanisms in plants to improve drought tolerance.

Mapping QTLs for yield components and chlorophyll a fluorescence parameters in wheat under three levels of water availability

2011 ◽  
Vol 9 (2) ◽  
pp. 291-295 ◽  
Author(s):  
Ilona Czyczyło-Mysza ◽  
Izabela Marcińska ◽  
Edyta Skrzypek ◽  
Małgorzata Chrupek ◽  
Stanisław Grzesiak ◽  
...  
Keyword(s):  
Drought Stress ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Wheat Yield ◽  
Dry Weight ◽  
Interval Mapping ◽  
Main Stem ◽  
Severe Drought ◽  
Single Marker Analysis ◽  
Fluorescence Parameters

Drought is one of the major factors limiting wheat yield in many developing countries worldwide. Parameters of chlorophyll a fluorescence kinetics under drought stress conditions have been used to characterize dehydration tolerance in wheat. In the present study, a set of 94 doubled haploid lines obtained from Chinese Spring × SQ1 (CSDH), mapped with 450 markers, was evaluated for yield (grain dry weight/main stem ear), number of grains/main stem ear (NG) and chlorophyll a fluorescence parameters (FC) under moderate and severe drought stress, and compared with results for well-watered plants. quantitative trait loci (QTLs) were identified using Windows QTLCartographer version 2.5 software and the results were analysed using single-marker analysis (SMA) and composite interval mapping (CIM). Analysis using SMA and CIM showed mostly similar QTLs for all traits, though more QTLs were identified by SMA than by CIM. The genetic control of yield, NG and FC varied considerably between drought-stressed and non-stressed plants. Although no major QTL co-locations were found for yield and FC using CIM, the co-location of QTLs for NG, yield and Fv/Fm in drought-stressed plants was observed on chromosome 5A using SMA.

scholarly journals Proteome Analysis of Date Palm (Phoenix dactylifera L.) under Severe Drought and Salt Stress

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Abdulrahman L. Al-Malki ◽  
Khalid O. Abulnaja
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Date Palm ◽  
Proteome Analysis ◽  
Rubisco Activase ◽  
2D Electrophoresis ◽  
Severe Drought ◽  
Protein Abundance ◽  
Low Protein

Date palm cultivars differently tolerate salinity and drought stress. This study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. Eighteen-month-old date palm plants were subjected to severe salt (48 g/L NaCl) and drought (82.5 g/L PEG or no irrigation) conditions for one month. Using a protein 2D electrophoresis method, 55 protein spots were analyzed using mass spectrometry. ATP synthase CF1 alpha chains were significantly upregulated under all three stress conditions. Changes in the abundance of RubisCO activase and one of the RubisCO fragments were significant in the same spots only for salt stress and drought stress with no irrigation, and oxygen-evolving enhancer protein 2 was changed in different spots. Transketolase was significantly changed only in drought stress with PEG. The expression of salt and drought stress genes of the chosen protein spots was either overexpressed or downexpressed as revealed by the high or low protein abundance, respectively. In addition, all drought tolerance genes due to no irrigation were downregulated. In conclusion, the proteome analysis of date palm under salinity and drought conditions indicated that both salinity and drought tolerance genes were differentially expressed resulting in high or low protein abundance of the chosen protein spots as a result of exposure to drought and salinity stress condition.

The isolation of genes from the resurrection grass Sporobolus stapfianus which are induced during severe drought stress

2000 ◽  
Vol 23 (3) ◽  
pp. 265-277 ◽  
Author(s):  
A. D. Neale ◽  
C. K. Blomstedt ◽  
P. Bronson ◽  
T.‐N. Le ◽  
K. Guthridge ◽  
...  
Keyword(s):  
Drought Stress ◽  
Severe Drought ◽  
Sporobolus Stapfianus

The GmXTH1 gene improves drought stress resistance of soybean seedlings

2021 ◽  
Vol 42 (1) ◽  
Author(s):  
Ye Zhang ◽  
Han-zhu Zhang ◽  
Jia-yu Fu ◽  
Ye-yao Du ◽  
Jing Qu ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Resistance ◽  
Soybean Seedlings ◽  
Drought Stress Resistance

scholarly journals Comparative Proteomics of Root Apex and Root Elongation Zones Provides Insights into Molecular Mechanisms for Drought Stress and Recovery Adjustment in Switchgrass

Proteomes ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 3 ◽  
Author(s):  
Zhujia Ye ◽  
Sasikiran Reddy Sangireddy ◽  
Chih-Li Yu ◽  
Dafeng Hui ◽  
Kevin Howe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drought Stress ◽  
Molecular Mechanisms ◽  
Root Zone ◽  
Root Apex ◽  
Root Tip ◽  
Leaf Water Content ◽  
Severe Drought ◽  
Proteomics Data ◽  
Drought Treatment

Switchgrass plants were grown in a Sandwich tube system to induce gradual drought stress by withholding watering. After 29 days, the leaf photosynthetic rate decreased significantly, compared to the control plants which were watered regularly. The drought-treated plants recovered to the same leaf water content after three days of re-watering. The root tip (1cm basal fragment, designated as RT1 hereafter) and the elongation/maturation zone (the next upper 1 cm tissue, designated as RT2 hereafter) tissues were collected at the 29th day of drought stress treatment, (named SDT for severe drought treated), after one (D1W) and three days (D3W) of re-watering. The tandem mass tags mass spectrometry-based quantitative proteomics analysis was performed to identify the proteomes, and drought-induced differentially accumulated proteins (DAPs). From RT1 tissues, 6156, 7687, and 7699 proteins were quantified, and 296, 535, and 384 DAPs were identified in the SDT, D1W, and D3W samples, respectively. From RT2 tissues, 7382, 7255, and 6883 proteins were quantified, and 393, 587, and 321 proteins DAPs were identified in the SDT, D1W, and D3W samples. Between RT1 and RT2 tissues, very few DAPs overlapped at SDT, but the number of such proteins increased during the recovery phase. A large number of hydrophilic proteins and stress-responsive proteins were induced during SDT and remained at a higher level during the recovery stages. A large number of DAPs in RT1 tissues maintained the same expression pattern throughout drought treatment and the recovery phases. The DAPs in RT1 tissues were classified in cell proliferation, mitotic cell division, and chromatin modification, and those in RT2 were placed in cell wall remodeling and cell expansion processes. This study provided information pertaining to root zone-specific proteome changes during drought and recover phases, which will allow us to select proteins (genes) as better defined targets for developing drought tolerant plants. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD017441.

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