scholarly journals Genetic Dissection of the Powdery Mildew Resistance in Wheat Breeding Line LS5082 Using BSR-Seq

2021 ◽  
Author(s):  
Liru Wu ◽  
Tong Zhu ◽  
Huagang He ◽  
Xinyou Cao ◽  
Haosheng Li ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Differentially Expressed Genes ◽  
Dominant Gene ◽  
Wheat Breeding ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Breeding Line ◽  
Seedling Resistance ◽  
Physical Position ◽  
Chromosome Arm

Abstract Wheat powdery mildew is a destructive disease seriously threatening yield and quality. Comprehensive dissection of new resistance-related loci/genes is necessary to control this disease. LS5082 is a Chinese wheat breeding line with resistance to powdery mildew. Genetic analysis indicated that a single dominant gene, tentatively designated PmLS5082, conferred seedling resistance to different Bgt isolates. Bulked segregant RNA-seq (BSR-seq) was carried out to map the R gene PmLS5082 and profile differentially expressed genes associated with PmLS5082. PmLS5082 was mapped to chromosome arm 2BL and flanked by the markers WGGBH612-5 and YTU19-005 with genetic distances of 0.3 and 0.4 cM, respectively. The physical position was subsequently locked into the interval of 710.3-711.0 Mb. PmLS5082 differs from the catalogued Pm genes on chromosome arm 2BL in its resistant spectrum, physical position and origin, suggesting it is most likely a new Pm gene. Through COG and KEGG analysis, differentially expressed genes associated with PmLS5082 were profiled, and six genes in PmLS5082 interval were confirmed to be associated with PmLS5082 via RT-qPCR, using an additional set of wheat samples and time-course analysis post-inoculation with Bgt. In order to transfer PmLS082 to different genetic backgrounds using marker-assisted selection (MAS), closely linked markers, including Kompetitive Allele-Specific PCR (KASP) markers, were evaluated and nine markers were confirmed to be widely applicable for MAS in different genetic backgrounds.

scholarly journals Characterization of a Powdery Mildew Resistance Gene in Wheat Breeding Line 10V-2 and Its Application in Marker-Assisted Selection

Plant Disease ◽  
2018 ◽  
Vol 102 (5) ◽  
pp. 925-931 ◽  
Author(s):  
Pengtao Ma ◽  
Hongxing Xu ◽  
Yunfeng Xu ◽  
Liping Song ◽  
Shuoshuo Liang ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Marker Assisted Selection ◽  
Ssr Marker ◽  
Dominant Gene ◽  
Wheat Breeding ◽  
Homologous Sequence ◽  
Agronomic Performance ◽  
Breeding Line ◽  
Related Gene ◽  
Seedling Resistance

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a serious disease of wheat (Triticum aestivum L.) throughout the world. Host resistance is the most effective and preferred means for managing this disease. Line 10V-2, a wheat breeding line with superior agronomic performance, shows broad-spectrum seedling resistance to powdery mildew. Genetic analysis demonstrated that its resistance was controlled by a single dominant gene, tentatively designated Pm10V-2. This gene was localized near the documented Pm2 locus on chromosome 5DS using the simple sequence repeat (SSR) marker Cfd81. To saturate the marker map of Pm10V-2, more markers were developed using bulked segregant RNA-Seq. Two single-nucleotide polymorphism (SNP) markers (Swgi047 and Swgi064), three expressed sequence tag markers (Swgi007, Swgi029, and Swgi038), and one SSR marker (Swgi066) were polymorphic between the resistant and susceptible bulks and showed tightly linked to the Pm10V-2 gene. Pm10V-2 was flanked by the new developed markers Swgi064 and Swgi066 at genetic distances of 0.4 and 1.2 centimorgans (cM), respectively, and cosegregated with Swgi007 and Swgi038. The homologous sequence of Pm2a was cloned from 10V-2 based on a recent study. Although the sequence cloned from 10V-2 was completely identical to that of the reported Pm2a-related gene, they did not cosegregate but were separated at a genetic distance of 1.6 cM, indicating that Pm10V-2 was different from the reported of Pm2a-related gene. When inoculated with multiple B. graminis f. sp. tritici isolates, Pm10V-2 had a significantly different resistance spectrum from Pm2a and other powdery mildew (Pm) resistance genes at or near the Pm2 locus. Therefore, Pm10V-2 may be a new Pm2 allele or Pm2-linked gene. To use Pm10V-2 in marker-assisted selection (MAS) breeding, seven markers applicable for MAS were confirmed, including three newly developed markers (Swgi029, Swgi038, and Swgi064) in the present work. Using these markers, a great number of resistant lines with desirable agronomic performance were selected from crosses involving 10V-2, including the breeding line KM5016, which has been entered in the Regional trials in Hebei Province, China.

Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and its Evaluation in Marker-Assisted Selection

Plant Disease ◽  
2021 ◽  
Author(s):  
Wenrui Wang ◽  
Huagang He ◽  
Huiming Gao ◽  
Hongxing Xu ◽  
Wenyue Song ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Wheat Breeding ◽  
Agronomic Performance ◽  
Breeding Line ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease seriously threatening yield and quality of common wheat (Triticum aestivum L., 2n=6x=42, AABBDD). Characterization of resistance genes against powdery mildew is useful in parental selection and for developing disease resistant cultivars. Chinese wheat breeding line KN0816 has superior agronomic performance and resistance to powdery mildew at all growth stages. Genetic analysis using populations of KN0816 crossed with different susceptible parents indicated that a single dominant gene, tentatively designated PmKN0816, conferred seedling resistance to different Bgt isolates. Using a bulked segregant analysis (BSA), PmKN0816 was mapped to the Pm6 interval on chromosome arm 2BL using polymorphic markers linked to the catalogued genes Pm6, Pm52, and Pm64, and flanked by markers CISSR02g-6 and CIT02g-2 both with genetic distances of 0.7 cM. Analysis of closely linked molecular markers indicated that the marker alleles of PmKN0816 differed from those of other powdery mildew resistance genes on 2BL, including Pm6, Pm33, Pm51, Pm64, and PmQ. Based on the genetic and physical locations and response pattern to different Bgt isolates, PmKN0816 is most likely a new powdery mildew resistance gene and confers effective resistance to all the 14 tested Bgt isolates. In view of the elite agronomic performance of KN0816 combined with the resistance, PmKN0816 is expected to become a valuable resistance gene in wheat breeding. To transfer PmKN0816 to different genetic backgrounds using marker-assisted selection (MAS), closely linked markers of PmKN0816 were evaluated and four of them (CIT02g-2, CISSR02g-6, CIT02g-10, and CIT02g-17) were confirmed to be applicable for MAS in different genetic backgrounds.

Isolation of differentially expressed genes in wheat caryopses with contrasting starch granule size

2013 ◽  
Vol 8 (3) ◽  
pp. 297-305
Author(s):  
Rita Armonienė ◽  
Kristina Jonavičienė ◽  
Vytautas Ruzgas ◽  
Gintaras Brazauskas
Keyword(s):  
Gene Expression ◽  
Winter Wheat ◽  
Differentially Expressed Genes ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Starch Granule ◽  
Wheat Breeding ◽  
Granule Size ◽  
Differentially Expressed ◽  
Breeding Lines

AbstractIn order to identify genes responsible for starch granule initiation during early development of wheat caryopsis, nine winter wheat breeding lines were studied. Two breeding lines, which are the most diverse in A-type granule size (26.85 µm versus 23.65 µm) were chosen for further differential gene expression analysis in developing caryopses at 10 and 15 days post-anthesis (DPA). cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis resulted in 384 transcript-derived fragments, out of which 18 were identified as being differentially expressed. Six differentially expressed genes, together with the six well-known starch biosynthesis genes, were chosen for semi-quantitative gene expression analysis in developing wheat caryopses at 10 and 15 DPA. This study provides genomic information on 18 genes differentially expressed at early stages of wheat caryopses development and reports on the identification of genes putatively involved in the production of large A-type granules. These genes are targets for further validation on their role in starch granule synthesis control and provide the basis for the development of DNA marker tools in winter wheat breeding for enhanced starch quality.

scholarly journals Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis

2020 ◽  
Vol 8 (10) ◽  
pp. 1621
Author(s):  
Guillaume Dubrulle ◽  
Adeline Picot ◽  
Stéphanie Madec ◽  
Erwan Corre ◽  
Audrey Pawtowski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mass Spectrometry ◽  
Protein Synthesis ◽  
Differentially Expressed Genes ◽  
Differentially Expressed ◽  
White Lupin ◽  
Post Inoculation ◽  
Infectious Process ◽  
Plant Infection ◽  
Colletotrichum Lupini

The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors for other Colletotrichum species during plant infection, and 377 hypothetical proteins. Simultaneously, a total of 304 proteins produced during the interaction were identified and quantified by mass spectrometry. Taken together, the results highlight that the dynamics of symptoms, gene expression and protein synthesis shared similarities to those of hemibiotrophic pathogens. In addition, a few genes with unknown or poorly-described functions were found to be specifically associated with the early or late stages of infection, suggesting that they may be of importance for pathogenicity. This study, conducted for the first time on a species belonging to the Colletotrichum acutatum species complex, presents an opportunity to deepen functional analyses of the genes involved in the pathogenicity of Colletotrichum spp. during the onset of plant infection.

scholarly journals Transcriptomic analysis reveals distinct resistant response by physcion and chrysophanol against cucumber powdery mildew

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1991 ◽  
Author(s):  
Yanping Li ◽  
Shilin Tian ◽  
Xiaojun Yang ◽  
Xin Wang ◽  
Yuhai Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Resistance ◽  
Powdery Mildew ◽  
Differentially Expressed Genes ◽  
Expression Patterns ◽  
Defense Responses ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Sequencing Data ◽  
Transcriptional Change

Physcion and chrysophanol induce defense responses against powdery mildew in cucumbers. The combination of these two compounds has synergistic interaction against the disease. We performed RNA-seq on cucumber leaf samples treated with physcion and chrysophanol alone and with their combination. We generated 17.6 Gb of high-quality sequencing data (∼2 Gb per sample) and catalogued the expressions profiles of 12,293 annotated cucumber genes in each sample. We identified numerous differentially expressed genes that exhibited distinct expression patterns among the three treatments. The gene expression patterns of the Chr and Phy treatments were more similar to each other than to the Phy × Chr treatment. The Phy × Chr treatment induced the highest number of differentially expressed genes. This dramatic transcriptional change after Phy × Chr treatment leaves reflects that physcion combined with chrysophanol treatment was most closely associated with induction of disease resistance. The analysis showed that the combination treatment caused expression changes of numerous defense-related genes. These genes have known or potential roles in structural, chemical and signaling defense responses and were enriched in functional gene categories potentially responsible for cucumber resistance. These results clearly demonstrated that disease resistance in cucumber leaves was significantly influenced by the combined physcion and chrysophanol treatment. Thus, physcion and chrysophanol are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to the defense response.

scholarly journals Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jian Wu ◽  
Qing Zhao ◽  
Qingyong Yang ◽  
Han Liu ◽  
Qingyuan Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Differentially Expressed Genes ◽  
Sclerotinia Sclerotiorum ◽  
Defense Response ◽  
Quantitative Resistance ◽  
Resistance Breeding ◽  
Mapk Signaling ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Sclerotinia Stem Rot

Abstract Sclerotinia stem rot caused by Sclerotinia sclerotiorum is one of the most devastating diseases in many important crops including Brassica napus worldwide. Quantitative resistance is the only source for genetic improvement of Sclerotinia-resistance in B. napus, but the molecular basis for such a resistance is largely unknown. Here, we performed dynamic transcriptomic analyses to understand the differential defense response to S. sclerotiorum in a resistant line (R-line) and a susceptible line (S-line) of B. napus at 24, 48 and 96 h post-inoculation. Both the numbers of and fold changes in differentially expressed genes in the R-line were larger than those in the S-line. We identified 9001 relative differentially expressed genes in the R-line compared with the S-line. The differences between susceptibility and resistance were associated with the magnitude of expression changes in a set of genes involved in pathogen recognition, MAPK signaling cascade, WRKY transcription regulation, jasmonic acid/ethylene signaling pathways and biosynthesis of defense-related protein and indolic glucosinolate. The results were supported by quantitation of defense-related enzyme activity and glucosinolate contents. Our results provide insights into the complex molecular mechanism of the defense response to S. sclerotiorum in B. napus and for development of effective strategies in Sclerotinia-resistance breeding.

scholarly journals Characterization of a Segregation Distortion Locus with Powdery Mildew Resistance in a Wheat-Thinopyrum intermedium Introgression Line WE99

Plant Disease ◽  
2016 ◽  
Vol 100 (8) ◽  
pp. 1541-1547 ◽  
Author(s):  
Pengtao Ma ◽  
Hongxing Xu ◽  
Guohao Han ◽  
Qiaoling Luo ◽  
Yunfeng Xu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Single Gene ◽  
Introgression Line ◽  
Triticum Aestivum L ◽  
Thinopyrum Intermedium ◽  
Seedling Resistance ◽  
Mildew Resistance ◽  
Genotype Frequencies ◽  
Chromosome Arm

Exploitation of host resistance is important for controlling powdery mildew of wheat (Triticum aestivum L.). In this study, a wheat-Thinopyrum intermedium introgression line, designated WE99, conferred seedling resistance to 47 of 49 Blumeria graminis f. sp. tritici isolates. Genetic analysis demonstrated that the resistance segregation deviated significantly from a single gene Mendelian ratio. However, marker analysis indicated that only a single recessive resistance gene, temporarily designated PmWE99, conferred powdery mildew resistance (Pm). PmWE99 was mapped to chromosome arm 2BS and linked to the three simple-sequence repeat markers Gwm148, Gwm271, and Barc55. Using race spectrum analysis, PmWE99 was shown to be significantly different from the documented genes Pm42 and MlIW170 located on chromosome arm 2BS and, thus, appeared to be a new Pm gene. Examination of the genotype frequencies in the F2:3 families showed that a genetic variation in the PmWE99 interval that favored the transmission of the WE99 allele could be the cause of the deviated segregation. Further investigation revealed that the abnormal segregation only occurred at the PmWE99 interval and was not common at other loci in this population. Identification of PmWE99 will increase the diversity of the Pm genes for wheat improvement.

scholarly journals QTL Mapping and Transcriptome Analysis to Identify Differentially Expressed Genes Induced by Septoria Tritici Blotch Disease of Wheat

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 510 ◽  
Author(s):  
Odilbekov ◽  
He ◽  
Armoniené ◽  
Saripella ◽  
Henriksson ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Differentially Expressed Genes ◽  
Resistance Genes ◽  
Transcriptome Analysis ◽  
Resistance Mechanism ◽  
Transcriptome Profiling ◽  
Wheat Breeding ◽  
Differentially Expressed ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici

Resistance to Septoria tritici blotch (STB) is an economically important trait in many wheat-breeding programs across the world. Several quantitative trait loci (QTL) for STB resistance were identified in wheat but due to the dynamic pathogen population it is necessary to continuously identify new resistance genes/QTL and determine the underlying resistance mechanism. In this work, we integrated QTL mapping and transcriptome profiling to identify candidate genes underlying QTL associated with STB resistance in bread wheat at the seedling stage. The results revealed four QTL on chromosomes 1BS, 1BL, 3AS and 3DL for STB resistance. Among these, two QTL on 2BL and 3DL were mapped for chlorosis, necrosis and pycnidia while the other two on 1BS and 3AS were associated with necrosis and pycnidia. Among the four identified QTL, genes were identified in three QTL (1BS, 2BL and 3DL). In total, 238 differentially expressed genes (DEGs) were localized in 1BS, 16 DEGs in 2BL and 80 DEGs in 3DL QTL region respectively. F-box protein, NBS-LRR disease resistance genes and receptor-like protein kinase were the most over-represented. The results emphasize the importance of integrating QTL and transcriptome analysis to accelerate the identification of key genes underlying the traits of interest.

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