scholarly journals Embryotoxicity and oxidative stress induced by arsenic in albino mice and protective role of Moringa oleifera

2021 ◽  
Author(s):  
Kashif Ali ◽  
Asia Iqbal ◽  
Talha Omer ◽  
Maham Saleem
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Protective Role ◽  
Abnormal Development ◽  
Sodium Arsenate ◽  
Leaf Extracts ◽  
Group A ◽  
Embryo Toxicity ◽  
Group D

Abstract Chronic exposure to arsenic causes the abnormal development of embryo during pregnancy. So the study is designed to investigate the antioxidant and tissue protective properties of Moringa oleifera extracts against sodium arsenate induced embryo toxicity. Moringa oleifera extracts (leaf and flower) were prepared using soxhelt apparatus. Fourty four pregnant females were equally divided in 11 experimental groups (A-K). Group A was of control while B and C were sodium arsenate treated groups as group A (0.00), B (6.00, 0.00), C (12.00, 0.00). Group D to G were of sodium arsenate + Moringa oleifera flower extract treated groups with doses D (6.00, 150.00), E (6.00, 300.00), F (12.00, 150.00), G (12.00, 300.00) and groups H to K were sodium arsenate + Moringa oleifera leaf extracts treated groups H (6.00, 150.00), I (6.00, 300.00), J (12.00, 150.00) and K (12.00, 300.00) mg/kg B.W. Significant (p < 0.05) increased in MDA (malanodialdehyde) values 41.75 ± 3.40 and decreased GSH (glutathione) values 7.75 ± 0.95 in sodium arsenate treated groups were observed as compared to control 25 ± 0.81(MDA) and 18.5 ± 0.57 (GSH). Moringa oleifera extracts treated groups especially Moringa oleifera leaf extract at dose of 300mg/kg B.W normalized the MDA (25.25 ± 0.50) and GSH (18.25 ± 1.70) values. Sodium arsenate induced the histopathologiacl changes like malformed heart, meningocoel, spina bifida, anopathalmia, cavitization, degenerated kidney and intestine. Whereas Moringa oleifera leaf extract as ameliorant minimized these histopathological abnormalities. It is concluded that Moringa oleifera leaf extract is mixture of biomolecules as it has antioxidant activity, so can be used as ameliorant against sodium arsenate.

scholarly journals Amelioration potential of Moringa oleifera extracts against sodium arsenate induced embryotoxicity and genotoxicity in mouse (Mus musculus)

2023 ◽  
Vol 83 ◽  
Author(s):  
K. Ali ◽  
A. Iqbal ◽  
S. M. Bukhari ◽  
S. Safdar ◽  
A. Raiz ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sodium Arsenate ◽  
Leaf Extracts ◽  
Flower Extract ◽  
Ameliorative Effect ◽  
Pregnant Mice

Abstract Previous studies have suggested that arsenic crosses the placenta and affects the fetus development. The study under consideration aims to show comparative ameliorative effect of Moringa oleifera leaf and flower extracts against sodium arsenate induced fetus toxicity of mice. Pregnant mice (N=44) were kept in lab and divided into eleven group from (A to K) and were orally administered the doses 6 mg/kg, 12 mg/kg for sodium arsenate, 150 mg/kg and 300 mg/kg for Moringa oleifera leaf extracts (MOLE) and 150 mg/kg and 300 mg/kg for Moringa oleifera flower extracts (MOFE) comparing with control. The investigation revealed evident reduction in the fetuses weight, hind limb, fore limb, tail and snout length, crown rump and head circumferences well as malformations in tail, feet, arms, legs, skin and eyes in the negative control group (only administered with sodium arsenate). Co-administration of sodium arsenate with MOLE and MOFE ameliorate the reversed effect of sodium arsenate on the shape, length, body weight and DNA damage of fetus significantly at 95% confidence interval. However, Moringa oleifera leaf extract showed more significant results in comparison to Moringa oleifera flower extract. Hence concluded that Moringa oleifera leaf extract ameliorated the embryo toxic effects of sodium arsenate and can be used against environmental teratogens.

scholarly journals Gambaran histopatologik hati tikus Wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi karbon tetraklorida (CCl4)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Satriani Syahrin ◽  
Carla Kairupan ◽  
Lily Loho
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Moringa Leaf Extract

Abstract: Moringa (Moringa oleifera) is a traditional plant, which is known to treat liver disease. The effect of moringa is related to the antioxidant activity of its compounds such as quercetin and flavonoid silymarin which are useful as liver protector. Carbon tetraclorida is a hepatotoxic substance that is usually used as an inducer of liver damage in studies related to hepatoprotector activity of a substance. This study aimed to describe liver histopathological features of Wistar rats fed with moringa leaf extract after CCl4 induction. This was an experimental study using 24 Wistar rats (Rattus norvegicus) which were divided into 4 groups. Group A, the negative control, was terminated at day 6th, and the other groups (B, C, and D) were induced with CCl4 0.05 cc/day for 5 days. After CCl4 induction, group B were terminated at day 6th; group C was treated with moringa leaf extract 100 mg/day for 5 days and was terminated at day 11th; group D received no treatment for 5 days and was terminated at day 11th. The results showed that groups induced with CCl4 for 5 days showed the presence of inflammatory cells and fatty cells. The groups treated with moringa leaf extract 100mg/day for 5 days after CCl4 induction 0.05 cc/day exhibited regeneration of liver cells in nearly all lobules. Conclusion: Administration of moringa leaf extract of 100mg/day could accelerate liver cell regeneration of Wistar rats after induction of CCl4 0.05cc/day.Keywords: moringa leaf extract, carbon tetrachloride, histopathologogical image of liverAbstrak: Kelor merupakan tanaman tradisional yang diketahui dapat mengobati penyakit hati. Khasiat obat tanaman kelor dihubungkan dengan kandungan senyawa kimia quercetin dan silymarin golongan flavonoid dengan aktivitas antioksidan yang dapat melindungi dan mengobati kerusakan hati. Karbon tetraklorida merupakan zat hepatotoksik yang lazim dipakai sebagai penginduksi kerusakan hati dalam pengujian aktivitas hepatoprotektor suatu zat. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik hati tikus wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi CCl4. Jenis penelitian ini ialah eksperimental menggunakan 24 ekor tikus Wistar spesies Rattus norvegicus yang dibagi dalam 4 kelompok. Kelompok A merupakan kelompok kontrol negatif, diterminasi pada hari ke-6; kelompok B,C, dan D (kelompok perlakuan) diberi CCl4 dengan dosis 0,05 cc/hari selama 5 hari. Setelah pemberian CCl4, kelompok B langsung diterminasi pada hari ke-6; kelompok C diberi ekstrak daun kelor 100 mg/hari selama 5 hari, diterminasi pada hari ke-11; kelompok D tidak diberi perlakuan selama 5 hari, diterminasi pada hari ke-11. Hasil penelitian menunjukkan bahwa kelompok tikus yang diinduksi CCl4 selama 5 hari secara histopatologik memperlihatkan sel-sel radang dan perlemakan sel. Kelompok tikus yang diberi ekstrak daun kelor 100 mg/hari selama 5 hari setelah diinduksi CCl4 0,05 cc/hari menunjukkan regenerasi sel hati, hampir di seluruh lobuli hati. Simpulan: Pemberian ekstrak daun kelor 100 mg/hari setelah induksi CCl4 0,05 cc/hari dapat mempercepat regenerasi sel hati tikus Wistar yang mengalami cedera akibat CCl4.Kata kunci: ekstrak daun kelor, karbon tetraklorida, gambaran histopatologik hati

scholarly journals HEPATOTOXICITY;

2017 ◽  
Vol 24 (08) ◽  
pp. 1200-1205
Author(s):  
Aamna Khokhar ◽  
Aisha Qayyum ◽  
Momina Khokhar
Keyword(s):  
Controlled Trial ◽  
Protective Role ◽  
Alanine Transaminase ◽  
Blood Samples ◽  
Medical College ◽  
Randomized Controlled ◽  
Group A ◽  
Group B ◽  
Group D

Objectives: To evaluate the protective role of silymarin against methotrexate(MTX) induced hepatotoxicity in mice. Study design: Randomized controlled trial on animalmodel. Period: 06 months from March 2016 to August 2016. Settings: Department ofPharmacology and Therapeutics, Army Medical College, Rawalpindi. Material and Methods:Thirty male BALB/c mice were randomly divided into five groups (n=6). Group A received 0.2 mlnormal saline intraperitoneally served as control for MTX. Group B received 0.2 ml distilled waterorally for 7 days served as control for oral silymarin. Group C received single intraperitonealinjection of MTX 20 mg/kg. Group D received silymarin 25 mg/kg orally for seven days. GroupE received silymarin 25 mg/kg orally for 7 days with MTX 20 mg/kg intraperitoneally at day 4.Blood samples for measuring serum ALT (Alanine Transaminase), AST (Aspartate transaminase)and ALP (Alkaline Phosphatase) along with liver samples for hepatic histological examinationwere taken after 24 hours of last dose. Results: Silymarin show hepatoprotective effect againstMTX induced hepatotoxicity. Conclusion: Silymarin has hepatoprotective potential whenadministered along with MTX.

scholarly journals Invasive Group A Streptococcal Disease in The Netherlands: Evidence for a Protective Role of Anti–Exotoxin A Antibodies

2000 ◽  
Vol 181 (2) ◽  
pp. 631-638 ◽  
Author(s):  
Ellen M. Mascini ◽  
Margriet Jansze ◽  
Joop F. P. Schellekens ◽  
James M. Musser ◽  
Joop A. J. Faber ◽  
...  
Keyword(s):  
The Netherlands ◽  
Protective Role ◽  
Exotoxin A ◽  
Invasive Group ◽  
Streptococcal Disease ◽  
Group A

scholarly journals Alleviation of carbon tetrachloride-induced hepatocellular damage and oxidative stress with a leaf extract of Glyphae brevis (Tiliaceae)

2018 ◽  
Vol 29 (6) ◽  
pp. 609-619 ◽  
Author(s):  
Lucky Legbosi Nwidu ◽  
Yibala I. Oboma ◽  
Ekramy Elmorsy ◽  
Wayne Grant Carter
Keyword(s):  
Carbon Tetrachloride ◽  
Leaf Extract ◽  
Protective Role ◽  
Lipid Peroxidation Product ◽  
Hematological Indices ◽  
Hepatocellular Damage ◽  
Antioxidant Parameters ◽  
Mechanistic Basis ◽  
Histopathological Studies

Abstract Background Glyphae brevis leaf is reported in ethnomedicine as a treatment for hepatitis and jaundice; however, no studies have hitherto investigated the mechanistic basis of these claims. Methods A hepato-protective role of G. brevis hydromethanolic (GBH) leaf extract was established against carbon tetrachloride (CCl4)-induced hepatotoxicity. Twenty-four hours after a CCl4 challenge, rats were sacrificed and serum hematological indices, lipid profile, and biochemical parameters were determined. The antioxidant enzymes parameters (glutathione, catalase, and superoxide dismutase) and lipid peroxidation product (thiobarbituric reactive substances) levels in liver homogenates were evaluated. Changes in the liver cyto-architecture of different treatment groups were also investigated. Results The GBH extract produced no significant impact on weight and hematological indices. Intoxication with CCl4 significantly (p<0.001–0.05) increased total cholesterol (TC) and low-density lipoproteins (LDL) compared with control rats. Pretreatment with GBH leaf extract significantly reduced triglycerides, TC, and LDL to approaching control levels (p<0.001–0.05). The GBH leaf extract significantly alleviated CCl4-induced elevation of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and the CCl4-induced depression of total protein, and albumin. Liver antioxidant parameters were significantly increased in plant extract-treated rats, and this antagonized the pro-oxidant effect of CCl4. Histopathological studies also supported a hepato-protective effect of GBH. Collectively, the GBH leaf extract alleviated the CCl4-induced hepatotoxicity through improvement of innate antioxidant enzyme levels and lipid metabolism and stabilized the hepatocyte cyto-architecture of intoxicated rats. Conclusions This study establishes the ethnomedicinal role of G. brevis leaf in hepatitis and the mechanistic basis of hepato-protection against CCl4-induced hepatotoxicity.

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