scholarly journals Simultaneous Improvement of Lincomycin a Production and Reduction of Lincomycin B Levels in Streptomyces Lincolnensis Using a Combined Medium Optimization Approach

2021 ◽  
Author(s):  
Xiu-hong Zheng ◽  
Rui-fang Ye ◽  
Qi-hang Ding ◽  
Feng-xian Hu ◽  
Hong-zhou Zhang ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Fermentation Medium ◽  
Production Costs ◽  
Pcr Analysis ◽  
Optimization Approach ◽  
Separation Processes ◽  
Surface Design ◽  
Steepest Ascent ◽  
Burman Design ◽  
Streptomyces Lincolnensis

Abstract PurposeThe current study aimed to optimize the culture and production parameters of industrial production of lincomycin A by Streptomyces lincolnensis using a statistical approach that could also reduce unwanted by-products. MethodsThe Plackett-Burman design, steepest ascent method, and response surface design were used to evaluate different factors that affect lincomycin A production. ResultsUsing an optimized S. lincolnensis fermentation medium, lincomycin A production was increased up to 4600 mg/L in shaking flasks, which indicated a 28.3% improvement over previous production in an un-optimized medium (3585 mg/L). Additionally, the concentration of lincomycin B by-product was reduced to 0.8%, which was 82.2% lower than that in the un-optimized medium. Further, quantitative real-time PCR analysis revealed the optimized medium improved lincomycin A production by stimulating key genes in the lincomycin A biosynthesis pathway, as well as an osmotic stress gene. ConclusionsOptimizing the fermentation medium improved lincomycin A production and decreased that of the lincomycin B by-product, which could help cut production costs and simplify downstream separation processes. We found that osmotic stress reduced the concentration of lincomycin B, which could also help reduce fermentation by-product yields in other actinobacteria.

scholarly journals Actinobacillus succinogenesATCC 55618 Fermentation Medium Optimization for the Production of Succinic Acid by Response Surface Methodology

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Li-Wen Zhu ◽  
Cheng-Cheng Wang ◽  
Rui-Sang Liu ◽  
Hong-Mei Li ◽  
Duan-Ji Wan ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Yeast Extract ◽  
Fermentation Medium ◽  
Basic Medium ◽  
Important Place ◽  
Steepest Ascent ◽  
Medium Components ◽  
Optimal Region ◽  
Bulk Chemical ◽  
Burman Design

As a potential intermediary feedstock, succinic acid takes an important place in bulk chemical productions. For the first time, a method combining Plackett-Burman design (PBD), steepest ascent method (SA), and Box-Behnken design (BBD) was developed to optimizeActinobacillus succinogenesATCC 55618 fermentation medium. First, glucose, yeast extract, and MgCO3were identified to be key medium components by PBD. Second, preliminary optimization was run by SA method to access the optimal region of the key medium components. Finally, the responses, that is, the production of succinic acid, were optimized simultaneously by using BBD, and the optimal concentration was located to be 84.6 g L−1of glucose, 14.5 g L−1of yeast extract, and 64.7 g L−1of MgCO3. Verification experiment indicated that the maximal succinic acid production of52.7±0.8 g L−1was obtained under the identified optimal conditions. The result agreed with the predicted value well. Compared with that of the basic medium, the production of succinic acid and yield of succinic acid against glucose were enhanced by 67.3% and 111.1%, respectively. The results obtained in this study may be useful for the industrial commercial production of succinic acid.

Optimization of Fermentation Conditions for Cellulase Production by HDZK-BYTF620 (Aureobasidium pullulans) which is from the Gut of Termites

2014 ◽  
Vol 692 ◽  
pp. 167-171
Author(s):  
Ming Jing Sun ◽  
Li Qing Sun ◽  
Shan Shan Wang ◽  
Kai Zhao
Keyword(s):  
Aureobasidium Pullulans ◽  
Fermentation Medium ◽  
Cellulase Production ◽  
Fermentation Conditions ◽  
Steepest Ascent ◽  
Burman Design ◽  
Major Factors ◽  
Initial Medium ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

The components of a fermentation medium were optimized to produce cellulose from HDZK-BYTF620 (Aureobasidium pullulans) which is from the gut of termites and can produce cellulose under liquid state fermentation conditions by response surface methodology. By Plackett-Burman design screened out three major factors of strains HDZK-BYTF620 which affected production of the activity cellulose, immediately, CaCl2, MgSO4and CMC-Na. The fermentation conditions were optimized with Box-Behnken experiment and the largest regional of the activity of cellulase was approach with the steepest ascent experiment. The optimum composition of medium addition of CaCl2, MgSO4and CMC-Na was 0.77 g/L, 0.41 g/L and 21.88 g/L, respectively. Under the optimum fermentation medium conditions, the activity of cellulase was (598.00±2.56)×10-3IU/mL, increased 5.0 % to that of using initial medium.

Medium Optimization for the Antibacterial Substances Production from Brevibacillus brevis XDH Using Response Surface Methodology

2011 ◽  
Vol 345 ◽  
pp. 355-360 ◽  
Author(s):  
Zhen Song ◽  
Xun Li Liu ◽  
Chang Xu Lu ◽  
Jian Yu ◽  
Rui Cheng Ju ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Antibacterial Activity ◽  
Response Surface ◽  
Medium Optimization ◽  
Fermentation Medium ◽  
Steepest Ascent ◽  
High Antibacterial Activity ◽  
Burman Design ◽  
Brevibacillus Brevis ◽  
Plackett Burman Design

Brevibacillus Brevis XDH is an antagonistic bacterium with high antibacterial activity against many pathogens. Fermentation medium for the production of the antibacterial substances was statistically optimized by Plackett-Burman design, steepest ascent experiments and response surface methodology. The optimized medium was composed of (g/L): glucose 15.6, soybean meal 28.4, starch 6, CaCO3 2 and MgSO4 4. After optimization, the production of antibacterial metabolites increased more than 70% than that of control.

scholarly journals Systematic design of separation process for bioethanol production from corn stover

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Suksun Amornraksa ◽  
Ittipat Subsaipin ◽  
Lida Simasatitkul ◽  
Suttichai Assabumrungrat
Keyword(s):  
Energy Consumption ◽  
Corn Stover ◽  
Process Design ◽  
Extractive Distillation ◽  
Separation Process ◽  
Production Costs ◽  
Bioethanol Production ◽  
Systematic Design ◽  
Separation Processes ◽  
Process Economics

Abstract Separation process is very crucial in bioethanol production as it consumes the highest energy in the process. Unlike other works, this research systematically designed a suitable separation process for bioethanol production from corn stover by using thermodynamic insight. Two separation processes, i.e., extractive distillation (case 2) and pervaporation (case 3), were developed and compared with conventional molecular sieve (case 1). Process design and simulation were done by using Aspen Plus program. The process evaluation was done not only in terms of energy consumption and process economics but also in terms of environmental impacts. It was revealed that pervaporation is the best process in all aspects. Its energy consumption and carbon footprint are 60.8 and 68.34% lower than case 1, respectively. Its capital and production costs are also the lowest, 37.0 and 9.88% lower than case 1.

scholarly journals Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Li-mei Lin ◽  
Ling-jia Zhao ◽  
Jing Deng ◽  
Su-hui Xiong ◽  
Jie Tang ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Steepest Ascent ◽  
Penthorum Chinense Pursh ◽  
Ft Ir ◽  
Experimental Yield ◽  
Burman Design ◽  
Anti Inflammatory ◽  
Penthorum Chinense ◽  
Optimized Conditions ◽  
Plackett Burman Design

Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.

scholarly journals Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method

2020 ◽  
Vol 12 (21) ◽  
pp. 8893
Author(s):  
Huanran Liu ◽  
Dan Zhang ◽  
Xia Zhang ◽  
Chuanzhi Zhou ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Spore Production ◽  
Steepest Ascent ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

The strains capable of degrading cellulose have attracted much interest because of their applications in straw resource utilization in solid-state fermentation (SSF). However, achieving high spore production in SSF is rarely reported. The production of spores from Streptomyces griseorubens JSD-1 was investigated in shaker-flask cultivation in this study. The optimal carbon, organic nitrogen and inorganic nitrogen sources were sucrose, yeast extract and urea, respectively. Plackett–Burman design (PBD) was adopted to determine the key medium components, and the concentration levels of three components (urea, NaCl, MgSO4·7H2O) were optimized with the steepest ascent path and central composite design (CCD), achieving 1.72 × 109 CFU/g of spore production. Under the optimal conditions (urea 2.718% w/v, NaCl 0.0697% w/v, MgSO4·7H2O 0.06956% w/v), the practical value of spore production was 1.69 × 109 CFU/g. The determination coefficient (R2) was 0.9498, which ensures an adequate credibility of the model.

scholarly journals Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Haixia Lu ◽  
Ke Yang ◽  
Lianghui Zhan ◽  
Tingting Lu ◽  
Xue Chen ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Dendrobium Officinale ◽  
Key Factors ◽  
Extraction Technology ◽  
Steepest Ascent ◽  
Burman Design ◽  
Solid Liquid

In order to establish the extraction technology of flavonoids from Dendrobium officinale leaves, a method combining Plackett–Burman design (PBD), steepest ascent design, and central composite design was developed to optimize the extraction of flavonoids. In addition, the tyrosinase activity inhibition of flavonoids was further tested in vitro. PBD results showed that ethanol concentration and number of extractions were key factors. Response surface methodology (RSM) indicated that the optimal extraction conditions were 78% ethanol concentration, six extraction times, 2 h, and 1:50 solid-liquid ratio. Under these conditions, the total flavonoid content could reach 35 mg/50 mL. In vitro tyrosinase experiment, the extracted total flavonoids had better inhibitory effect on tyrosinase activity than β-arbutin, and its inhibition rate for monophenolase and diphenolase exceeded 100% and 70%, respectively. These results indicate that RSM can effectively improve the extraction of flavonoids from Dendrobium officinale leaves and the flavonoids have the prospect of being applied to foods and cosmetics.

Enhanced Production of Mycelia by the Medicinal Mushroom Cordyceps militaris Using Plackett-Burman Design and Response Surface Methodology

2011 ◽  
Vol 138-139 ◽  
pp. 1209-1214
Author(s):  
Xiao Yu Liu ◽  
Fan Xing Meng ◽  
Yi Bo Zhang ◽  
Huan He ◽  
Wei Han ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Dry Weight ◽  
Fermentation Medium ◽  
Cordyceps Militaris ◽  
Enhanced Production ◽  
Burman Design ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

Response surface methodology (RSM) was used for statistical optimization of fermentation medium that influenced the yield of endo-polysaccharide from cultivated mycelia of Cordyceps militaris. First, the Plackett-Burman design was used to evaluate the effects of ten variables including glucose, maltose, peptone, yeast extract, KH2PO4, MgSO4, CaCl2, VB1, inoculum density and medium capacity. Among these variables, glucose, peptone and yeast extract were identified to have the significant effects. Subsequently, response surface methodology based on a five-level three-factor central composite design was employed to determine the maximum dry weight (DW) of mycelial biomass at optimum concentration of glucose, peptone and yeast extract. The mycelia growth was found to correlate to the three parameters that could be represented by second-order polynomial models. The optimal values of the three parameters were determined as 4.62% glucose, 3.36% peptone and 0.43% yeast extract. The prediction DW was 23.727g/L. The actual experimental results were in agreement with the prediction.

scholarly journals Rational Design of Ion Separation Membranes

2019 ◽  
Author(s):  
Matthias Wessling
Keyword(s):  
Rational Design ◽  
Water Flux ◽  
Membrane Properties ◽  
Optimization Approach ◽  
Layer By Layer ◽  
Separation Processes ◽  
Ion Separation ◽  
Separation Membranes ◽  
Membrane Fabrication ◽  
Artificial Neural Network Ann

Synthetic membranes for desalination and ion separation processes are a prerequisite for the supply of safe and sufficient drinking water as well as smart process water tailored to its application. This requires a versatile membrane fabrication methodology. Starting from an extensive set of new ion separation membranes synthesized with a layer-by-layer methodology, we demonstrate for the first time that an artificial neural network (ANN) can predict ion retention and water flux values based on membrane fabrication conditions. The predictive ANN is used in a local single-objective optimization approach to identify manufacturing conditions that improve permeability of existing membranes. A deterministic global multi-objective optimization is performed in order to identify the upper bound (Pareto front) of the delicate trade-off between ion retention characteristics and permeability. Ultimately, a coupling of the ANN into a hybrid model enables physical insight into the influence of fabrication conditions on apparent membrane properties.

scholarly journals Purification of Polymer-Grade Fumaric Acid from Fermented Spent Sulfite Liquor

2017 ◽  
Author(s):  
Diogo Figueira ◽  
João Cavalheiro ◽  
Bruno Sommer Ferreira
Keyword(s):  
Fumaric Acid ◽  
Process Integration ◽  
Fermentation Broth ◽  
Arable Land ◽  
Fermentation Medium ◽  
Production Costs ◽  
Raw Material ◽  
Separation Unit ◽  
Polymer Industry ◽  
Spent Sulfite Liquor

Fumaric acid is a chemical building block with many applications, namely in the polymer industry. The fermentative production of fumaric acid from renewable feedstock is a promising and sustainable alternative to petroleum-based chemical synthesis. The use of existing industrial side-streams as raw-material within biorefineries potentially enable production costs competitive against current chemical processes, while preventing the use of refined sugars competing with food and feed uses and avoiding purposely grow crops requiring large areas of arable land. However, most industrial side streams contain a diversity of molecules that will add complexity to the purification of fumaric acid from the fermentation broth. A process for the recovery and purification of fumaric acid from complex fermentation medium containing spent sulfite liquor (SSL) as carbon source was developed and is herein described. A simple two-stage precipitation procedure involving separation unit operations, pH and temperature manipulation and polishing through the removal of contaminants with activated carbon allowed the recovery of fumaric acid with 68.3% recovery yield with specifications meeting the requirements of the polymer industry. Further, process integration opportunities were implemented that allowed minimizing the generation of waste streams containing fumaric acid which enabled increasing the yield to 81.4% while keeping the product specifications.

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