scholarly journals Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Haixia Lu ◽  
Ke Yang ◽  
Lianghui Zhan ◽  
Tingting Lu ◽  
Xue Chen ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Dendrobium Officinale ◽  
Key Factors ◽  
Extraction Technology ◽  
Steepest Ascent ◽  
Burman Design ◽  
Solid Liquid

In order to establish the extraction technology of flavonoids from Dendrobium officinale leaves, a method combining Plackett–Burman design (PBD), steepest ascent design, and central composite design was developed to optimize the extraction of flavonoids. In addition, the tyrosinase activity inhibition of flavonoids was further tested in vitro. PBD results showed that ethanol concentration and number of extractions were key factors. Response surface methodology (RSM) indicated that the optimal extraction conditions were 78% ethanol concentration, six extraction times, 2 h, and 1:50 solid-liquid ratio. Under these conditions, the total flavonoid content could reach 35 mg/50 mL. In vitro tyrosinase experiment, the extracted total flavonoids had better inhibitory effect on tyrosinase activity than β-arbutin, and its inhibition rate for monophenolase and diphenolase exceeded 100% and 70%, respectively. These results indicate that RSM can effectively improve the extraction of flavonoids from Dendrobium officinale leaves and the flavonoids have the prospect of being applied to foods and cosmetics.

scholarly journals Tyrosinase Inhibitory Effect, Antioxidant and Anticancer Activities of Bioactive Compounds in Ripe Hog Plum (Spondias Pinnata) Fruit Extracts

2019 ◽  
Vol 35 (3) ◽  
pp. 916-926
Author(s):  
Supawadee Patathananone ◽  
Jureerut Daduang ◽  
Amonrat Koraneekij ◽  
Chia-Ying Li
Keyword(s):  
High Performance ◽  
Antioxidant Property ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Normal Fibroblast ◽  
Anticancer Activities ◽  
Ic50 Value ◽  
Bioactive Agents ◽  
Hog Plum

The usage of ripe hog plum fruit (Spondias pinnata) extracts in cosmetics and food products, including cancer therapeutic agents, have a few studies. Herein, the strong anti-tyrosinase activity found in the extracted part of isopropanol is reported. This extract was separated by liquid/liquid extraction using hexane: methanol+H2O. The hydrophilic layer (6A*) exhibited the anti-tyrosinase, antioxidant, and anticancer activities in vitro. The IC50 value of each bioactivity was presented as approximately 0.18, 0.04, and 1.40 mg/ml, respectively. In addition, 6A* fraction showed a very low cytotoxic effect in normal fibroblast cells (NHDF cells). The bioactive agents in 6A* were purified by C18 reverse-phase High-Performance Liquid Column Chromatography (HPLC). The 12 purified peaks were shown in the chromatogram profile. All peaks (excepted 6A-06 and 6A-09) displayed anti-tyrosinase activity, whereas the antioxidant property was not found in 6A-01, 6A-06, and 6A-08 but was represented in other peaks. Most purified peaks were indicated to be the aromatic alcohol or derivative phenol compounds.

scholarly journals Non-alcoholic components of Pelinkovac, a Croatian wormwood-based strong liquor, counteract the inhibitory effect of high ethanol concentration on catalase in vitro

2022 ◽  
Author(s):  
Jan Homolak ◽  
Ana Babic Perhoc ◽  
Mihovil Joja ◽  
Ivan Kodvanj ◽  
Karlo Toljan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Ethanol Concentration ◽  
Inhibitory Effect ◽  
Alcoholic Beverages ◽  
Acidic Ph ◽  
Inhibitory Effects ◽  
High Ethanol ◽  
Antioxidant Enzyme Catalase

Antioxidant enzyme catalase protects the cells against alcohol-induced oxidative stress by scavenging free radicals and metabolizing alcohol. Concentrations of ethanol present in alcoholic beverages can inhibit catalase and foster oxidative stress and alcohol-induced injury. Non-alcoholic components of pelinkovac counteract the inhibitory effects of high ethanol concentration and acidic pH on catalase in vitro.

Optimisation of the Ethanol-Based Heat Reflux Extraction of Isoflavones from Pueraria lobata (Willd.) Ohwi Root

2014 ◽  
Vol 955-959 ◽  
pp. 180-186 ◽  
Author(s):  
Shu Ya Wang ◽  
Jun Gao ◽  
Yi Lu Chen ◽  
Jun Zhou ◽  
Xiao Yu Yong ◽  
...  
Keyword(s):  
Large Scale ◽  
Ethanol Concentration ◽  
Orthogonal Experiment ◽  
Extraction Yield ◽  
Pueraria Lobata ◽  
Scale Production ◽  
Extraction Temperature ◽  
Extraction Technology ◽  
Large Scale Production ◽  
Solid Liquid

Ethanol-based heat reflux extraction has been successfully applied in extracting isoflavone compounds from the dried root of Pueraria lobata (Willd.) Ohwi. The different extraction factors including ethanol concentration, extraction temperature, extraction time, ratio of solid/liquid and number of extraction cycles has been studied on the influence of the extraction yield of isoflavone compounds. And the orthogonal experiment has been used to optimize the extracting conditions. A variety of indicators were employed to evaluate the extraction efficiency, including the yield of puerarin, daidzein and total isoflavone. The optimal extraction technology was as follows: extraction temperature 90°C, ratio of solid/liquid 1:6, ethanol concentration 90% and extraction 2 h. Under the optimal conditions extraction rates of puerarin, daidzein and total isoflavone were 37.51±0.64 mg/g, 2.63±0.03 mg/g and 117.4±0.89 mg/g. The study of this article can provide theoretical basis for large-scale production of puerarin, daidzein and total isoflavone.

scholarly journals Inhibitory Effect of Arctigenin from Fructus Arctii Extract on Melanin Synthesis via Repression of Tyrosinase Expression

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Hwayong Park ◽  
Kwang Hoon Song ◽  
Pil Mun Jung ◽  
Ji-Eun Kim ◽  
Hyunju Ro ◽  
...  
Keyword(s):  
Active Compound ◽  
Gene Promoter ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Dependent Manner ◽  
Melanin Content ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal

To identify the active compound arctigenin in Fructus Arctii (dried seed of medicinal plantArctium lappa) and to elucidate the inhibitory mechanism in melanogenesis, we analyzed melanin content and tyrosinase activity on B16BL6 murine melanoma and melan-A cell cultures. Water extracts of Fructus Arctii were shown to inhibit tyrosinase activity in vitro and melanin content inα-melanocyte stimulating hormone-stimulated cells to similar levels as the well-known kojic acid and arbutin, respectively. The active compound arctigenin of Fructus Arctii displayed little or no cytotoxicity at all concentrations examined and decreased the relative melanin content and tyrosinase activity in a dose-dependent manner. Melanogenic inhibitory activity was also identified in vivo with zebrafish embryo. To determine the mechanism of inhibition, the effects of arctigenin on tyrosinase gene expression and tyrosinase promoter activity were examined. Also in addition, in the signaling cascade, arctigenin dose dependently decreased the cAMP level and promoted the phosphorylation of extracellular signal-regulated kinase. This result suggests that arctigenin downregulates cAMP and the tyrosinase enzyme through its gene promoter and subsequently upregulates extracellular signal-regulated kinase activity by increasing phosphorylation in the melanogenesis signaling pathway, which leads to a lower melanin content.

Study on Microwave-Assisted Extraction Technology of Total Flavonoid from Castor Leaves

2014 ◽  
Vol 1004-1005 ◽  
pp. 868-872
Author(s):  
Yi Hua Jiang ◽  
Xin Long Jiang ◽  
Cheng Gang Cai
Keyword(s):  
Ethanol Concentration ◽  
Orthogonal Experiment ◽  
Total Flavonoids ◽  
Microwave Assisted Extraction ◽  
Extraction Technology ◽  
Power Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Irradiation Power ◽  
Solid Liquid

Orthogonal experiment optimization of microwave-assisted extraction (MAE) conditions of total flavonoids from castor leaves with the factors of ethanol concentration, microwave power, extraction time, solid/liquid ratio on the yield of total flavonoids were studied. The results showed that optimum extracting conditions of total flavonoids from castor leaves were as follows: adding 1:50 g·mL-1 of 40% alcohol and extracting for 3 min,microwave irradiation power 464W. Microwave extraction of total flavonoids from castor leaves is fast, energy saving and high extraction rate.

Extraction Technology and Determination of Total Flavonoid in Magnolia Liliiflora Petals

2014 ◽  
Vol 941-944 ◽  
pp. 1003-1007 ◽  
Author(s):  
Yi Hua Jiang ◽  
Xin Long Jiang ◽  
Cheng Gang Cai
Keyword(s):  
Ethanol Concentration ◽  
Orthogonal Experiment ◽  
Total Flavonoids ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Assisted Extraction ◽  
Irradiation Power ◽  
Maximal Yield ◽  
Solid Liquid

Orthogonal experiment optimization of microwave-assisted extraction (MAE) conditions of total flavonoids from Magnolia liliflora petals with the factors of ethanol concentration, extraction time,microwave power,solid/liquid ratio on the yield of total flavonoids were studied. The results showed that optimum extracting conditions of total flavonoids from Magnolia liliflora were as follows: adding 1:10 g·mL-1 of 40% alcohol and extracting for 5 min,microwave irradiation power 464W, under the optimal conditions, the maximal yield of flavonoids was of 2.08%.

The effects of condensed tannins from Dorycnium rectum on skatole and indole ruminal biogenesis for grazing sheep

2005 ◽  
Vol 56 (12) ◽  
pp. 1331 ◽  
Author(s):  
Michael H. Tavendale ◽  
Geoffrey A. Lane ◽  
Nicola M. Schreurs ◽  
Karl Fraser ◽  
Lucy P. Meagher
Keyword(s):  
Indole Acetic Acid ◽  
Condensed Tannin ◽  
Inhibitory Effect ◽  
Forage Legume ◽  
Key Factors ◽  
In Vitro Fermentation ◽  
Rumen Microbes ◽  
Indolic Compounds ◽  
Selection Of

Skatole and indole are flavour compounds formed in the rumen, which are key factors in the pastoral flavour of meat and milk products. A selection of indolic compounds has been screened for their potential to form skatole and indole on in vitro fermentation, with rumen inocula collected from New Zealand pasture-fed sheep. Typically, 8% (P < 0.005) of added tryptophan was converted to indole and 54% (P < 0.001) was converted to skatole via indole acetic acid (IAA). The addition to rumen inocula of a condensed tannin (CT) fraction isolated from the forage legume, Dorycnium rectum, reduced the conversion of plant protein to skatole and indole by 75% and specifically inhibited the transformation of IAA to skatole by 85% (P < 0.001). The conversion of tryptophan to indole or IAA was not affected by CTs. The inhibitory effect of CTs on the conversion of tryptophan to skatole was prevented by the addition of polyethylene glycol (PEG) to the rumen inocula prior to the addition of CTs, but not by the addition of PEG subsequent to CTs, demonstrating that interactions between CTs and microbes involved in skatole biosynthesis are not reversible by PEG. This study is the first to demonstrate that isolated CTs have an inhibitory effect on the conversion of protein to skatole and indole by rumen microbes, and that CTs specifically inhibit the transformation of IAA to skatole by rumen microbes.

scholarly journals OPTIMIZATION OF BREWING WASTE’S (TRUB) PHENOLIC COMPOUNDS EXTRACTION BY ULTRASOUND ASSISTED USING RESPONSE SURFACE METHODOLOGY

Química Nova ◽  
2020 ◽  
Author(s):  
Bianca Gandolpho ◽  
Aline Almeida ◽  
Gabriel Gandolpho ◽  
Daniele Freitas ◽  
Otávio Gasparini ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Operating Conditions ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Ultrasound Assisted ◽  
Solid Liquid

The brewing waste, also known as trub, is an abundant by-product of the brewing industry. Such material presents high levels of phenolic compounds, which promote antioxidant, antimicrobial and antifungal effects, turning the trub economically attractive. In this study, the trub’s phenolic compounds were extracted by ultrasound-assisted extraction technology. Such experiments were conducted according to a central composite rotatable design (CCRD), with the evaluated parameters being ethanol concentration, solid-liquid ratio and extraction temperature. Response surface methodology (RSM) described the effect of process’ variables through second order polynomial models, adjusted appropriately for such analysis, and optimized the operating conditions, aiming to obtain the maximum extraction of phenolic compounds through the proposed technique. The extraction’s optimal conditions for the evaluated variables were ethanol concentration of 58%, solid-liquid ratio of 1 g per 32 mL, and extraction temperature of 36 ºC, during a 30 minute process. Considering such experimental conditions, the total amount of phenolic compounds was equal to 7.23 mg of gallic acid g-1 trub, indicating that a great concentration of phenolic compounds can be extracted from this material through the proposed technique. Thus, this indicates that trub might be a promising by-product that can be used in different industrial fields.

Optimization of Extraction Technology of Favonoids from Kandelia Candel Leaves by Using Response Surface Methodology

2013 ◽  
Vol 704 ◽  
pp. 332-337
Author(s):  
Yan Ping Ji ◽  
Cheng Ping Li ◽  
Xing Xu ◽  
Lan Tang ◽  
Qian Tong
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Kandelia Candel ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Box Behnken Design ◽  
Extraction Parameters ◽  
Solid Liquid

This work is to extract flavonoids using ethanol refluxing method from the Kandelia candel leaves which are rich in flavonoids. Ethanol concentration, solid-liquid ratio, temperature and extraction time on the extraction rate were investigated by the single factor experiments. Box-Behnken design (BBD) based on response surface methodology (RSM) was adopted to optimize the parameters. The results showed the best extracting rate was more than 7.74%, when the extraction parameters were ethanol concentration 60%, extraction time 0.5 h and temperature 80°C.

scholarly journals Collagenase and Tyrosinase Inhibitory Effect of Isolated Constituents from the Moss Polytrichum formosum

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1271
Author(s):  
Raíssa Volpatto Marques ◽  
Agnès Guillaumin ◽  
Ahmed B. Abdelwahab ◽  
Aleksander Salwinski ◽  
Charlotte H. Gotfredsen ◽  
...  
Keyword(s):  
Biological Activities ◽  
Ethanol Extract ◽  
Binding Mode ◽  
Inhibitory Effect ◽  
Skin Aging ◽  
Tyrosinase Activity ◽  
Specific Affinity ◽  
Dose Dependent ◽  
Polytrichum Formosum

Mosses from the genus Polytrichum have been shown to contain rare benzonaphthoxanthenones compounds, and many of these have been reported to have important biological activities. In this study, extracts from Polytrichum formosum were analyzed in vitro for their inhibitory properties on collagenase and tyrosinase activity, two important cosmetic target enzymes involved respectively in skin aging and pigmentation. The 70% ethanol extract showed a dose-dependent inhibitory effect against collagenase (IC50 = 4.65 mg/mL). The methanol extract showed a mild inhibitory effect of 44% against tyrosinase at 5.33 mg/mL. Both extracts were investigated to find the constituents having a specific affinity to the enzyme targets collagenase and tyrosinase. The known compounds ohioensin A (1), ohioensin C (3), and communin B (4), together with nor-ohioensin D (2), a new benzonaphthoxanthenone, were isolated from P. formosum. Their structures were determined by mass spectrometry and NMR spectroscopy. Compounds 1 (IC50 = 71.99 µM) and 2 (IC50 = 167.33 µM) showed inhibitory activity against collagenase. Compound 1 also exhibited inhibition of 30% against tyrosinase activity at 200 µM. The binding mode of the active compounds was theoretically generated by an in-silico approach against the 3D structures of collagenase and tyrosinase. These current results present the potential application from the moss P. formosum as a new natural source of collagenase and tyrosinase inhibitors.

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