Left Supraclavicular (Virchow’s) Node Metastasis Detected Before Diagnosis of the Primary Infradiaphragmatic Tumor

Abstract Background and Purpose: Malignant infradiaphragmatic tumors that metastasize to the left supraclavicular (Virchow’s) node are relatively rare. Since Virchow's time, the survival rates of cancer patients have improved because of dramatic research advances in the epidemiology of cancer and the development of screening technologies and treatment methods. However, when metastasis is detected in the left supraclavicular node in patients with head and neck carcinoma, locating the primary cancer remains a difficult and time-consuming challenge. We report three cases of malignant infradiaphragmatic tumors diagnosed following the initial finding of left supraclavicular node (LSN) metastasis after surgery for tongue squamous cell carcinoma (follow-up period, range 18–62 months).Methods: In these cases, adenocarcinoma was diagnosed based on LSN biopsies, and a second primary tumor was found, including cholangiocarcinoma, bladder carcinoma, and prostate carcinoma.Results: CA19-9 and alpha-fetoprotein levels helped to diagnose cholangiocarcinoma. In the bladder carcinoma case, immunopathological examination of the metastatic LSN and bladder lesion showed GATA 3(+), and the tongue squamous cell carcinoma showed GATA 3(-). GATA 3 staining aided in the diagnosis of urothelial bladder cancer with LSN metastasis. In the prostate carcinoma case, PSA levels and weak FDG-PET/CT uptake aided the diagnosis process. This patient underwent bilateral orchiectomy and adjuvant hormonal therapy and survived for 47 months after the diagnosis of LSN metastasis. The other patients died within 1 year.Discussion and Conclusions: These cases suggest that the prognosis of malignant infradiaphragmatic tumors after LSN metastasis depends on the tumor characteristics and that rapid diagnosis is important for providing appropriate treatment.

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TERT promoter mutation in patients with second primary of tongue squamous cell carcinoma

Oral Oncology ◽

10.1016/j.oraloncology.2020.105089 ◽

2020 ◽

pp. 105089

Author(s):

Evren Erkul ◽

Ismail Yilmaz

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Tongue Squamous Cell Carcinoma ◽

Second Primary ◽

Tert Promoter Mutation ◽

Promoter Mutation ◽

Tert Promoter

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Feasibility of dpFAMM flap in Tongue Reconstruction After Facial Vessels Ligation and Radiotherapy- Case Presentation

10.21203/rs.3.rs-1039084/v1 ◽

2021 ◽

Author(s):

Gontarz Michał ◽

Jakub Bargiel ◽

Krzysztof Gąsiorowski ◽

Tomasz Marecik ◽

Paweł Szczurowski ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Neck Dissection ◽

Squamous Cell ◽

Salvage Surgery ◽

Tongue Cancer ◽

Tongue Squamous Cell Carcinoma ◽

Second Primary ◽

Case Presentation ◽

Tongue Reconstruction

Abstract BackgroundSalvage surgery with reconstruction of the second and next primary tongue cancer remains difficult, especially after earlier neck dissection and radiotherapy. In the current report, we describe the feasibility of the extended, double-pedicled facial artery musculomucosal (dpFAMM) flap in the reconstruction of the patient with second primary tongue squamous cell carcinoma, after facial vessels ligation and radiotherapy. Case presentation81-year-old female patient was operated on due to tongue squamous cell carcinoma (SCC) on the left side T3N1M0 in 2019. Bilateral selective neck dissection with tongue reconstruction was performed by island FAMM flap. The patient also suffered from synchronous mucinous breast carcinoma treated with tamoxifen. Second primary SCC of the tongue on the opposite (right) side was detected in 2020. The patient did not agree for surgical treatment therefore radiotherapy was performed. Local recurrence of the tongue cancer of the right side was treated surgically in 2021. Salvage surgery comprised hemiglossectomy and dpFAMM flap reconstruction with uneventful postoperative follow-up.ConclusionsThis case presentation proved that dpFAMM flap can be used in salvage surgery and reconstruction even in patients after ligation of facial vassels, irradiation and in the course of hormone therapy. The flap is easy to handle, has good vascularity and comprises predictable method of reconstruction, especially for patients with severe comorbidities.

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Hubungan ekspresi p53, Bcl-2, c-Myc dan MMP-9 dengan gambaran klinikopatologi karsinoma sel skuamosa kepala-leher

Oto Rhino Laryngologica Indonesiana ◽

10.32637/orli.v46i1.147 ◽

2016 ◽

Vol 46 (1) ◽

pp. 53

Author(s):

Azwar Azwar ◽

Sofia Mubarika ◽

Agus Surono

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Tumor Location ◽

Neck Squamous Cell Carcinoma ◽

Second Primary ◽

Cross Sectional ◽

P53 Expression ◽

Local Metastasis

Latar belakang: Karsinoma sel skuamosa kepala dan leher merupakan salah satu kanker terseringdi seluruh dunia. Pendekatan pengobatan agresif dan multidisiplin telah dilakukan, namun belum adapeningkatan yang signifikan dalam kelangsungan hidup 5 tahun, selama 20 tahun terakhir. Kegagalanpengobatan terjadi dalam bentuk kekambuhan lokoregional, metastasis jauh, dan/atau tumor primer kedua.Berbagai penanda molekular tumor telah diteliti untuk mengetahui potensinya dalam memprediksi hasilpenyakit atau respon terhadap terapi.Tujuan: Mengetahui hubungan ekspresi protein p53, Bcl-2, c-Myc,dan MMP-9 berdasarkan gambaran klinikopatologis karsinoma sel skuamosa kepala dan leher di RumahSakit dr. Zainoel Abidin.Metode: Studi menggunakan desain cross sectional. Sampel terdiri dari 60blok parafin karsinoma sel skuamosa kepala dan leher. Prosedur pewarnaan imunohistokimia dilakukandengan menggunakan antibodi monoklonal terhadap p53, Bcl-2, c-Myc, dan MMP-9. Ekspresi proteinp53, Bcl-2, c-Myc, dan MMP-9 dianalisis secara imunohistokimia pada karsinoma sel skuamosa kepaladan leher kemudian hasilnya dihubungkan dengan parameter klinikopatologis seperti usia, jenis kelamin,lokasi tumor, diferensiasi tumor, metastasis kelenjar getah bening dan stadium tumor, kemudian dianalisisstatistik dengan Chi square.Hasil: Hasil penelitian menunjukkan terdapat hubungan bermakna tingkatekspresi p53 dengan metastasis lokal (p=0,021) dan ada hubungan bermakna tingkat ekspresi MMP-9dengan lokasi tumor (p=0,026). Tidak terdapat hubungan ekspresi p53, Bcl-2, cMyc, dan MMP-9 terhadapusia, jenis kelamin, stadium tumor, diferensiasi histologi, tingkat T, N, dan metastasis jauh.Kesimpulan:Ada hubungan ekpresi p53 dengan metastasis kelenjar limfe regional dan ekspresi MMP-9 dengan lokasitumor pada karsinoma sel skuamosa kepala dan leher. Kata kunci: Karsinoma sel skuamosa kepala dan leher, p53, Bcl-2, c-Myc, MMP-9 ABSTRACTBackground: Head and neck squamous cell carcinoma (HNSCC) is one of the most commoncancers world wide. Although aggressive and multidisciplinary approach to the treatment has been done,there is no significant improvement in 5-year survival in the last 20 years. Treatment failure occurredin the form of locoregional recurrence, distant metastasis, and/or a second primary tumor. A variety oftumor molecular markers have been studied to determine their potential in predicting disease outcome orresponse to the therapy. Purpose: To investigate correlation p53, Bcl-2, c-Myc, and MMP-9 expressionto clinicopathologic parameter in head and neck squamous cell carcinoma patient in dr. Zainoel Abidinhospital. Methods: Cross sectional design study. The sample was consisted of 60 paraffin blocks ofhead and neck squamous cell carcinoma. Procedure of immunohistochemical staining used monoclonalantibodies against p53, Bcl-2, c-Myc, and MMP-9. Expression of p53 protein, Bcl-2, c-Myc, and MMP-9were analyzed by immunohistochemistry in head and neck squamous cell carcinoma. Then, the results were linked to clinicopathologic parameters such as age, sex, tumor location, tumor differentiation,lymph node metastasis and tumor stage, and statistically analyzed with Chi square. Results: The resultsshowed there were significant correlation between p53 expression level with local metastasis (p=0,021)and significant correlation of MMP-9 expression levels with tumor location (p=0,026). There were norelationship of p53, Bcl-2, cMyc and MMP-9 expressions based on age, sex, stage tumor, histologicdifferentiation, level of T, N, and distant metastases. Conclusion: There were relationships between p53expression with local metastasis and MMP-9 expression with tumor location in head and neck squamouscell carcinoma. Keywords: Head and neck squamous cell carcinoma, p53, Bcl-2, c-Myc, MMP-9

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Isoliquiritigenin Inhibits Proliferation and Induces Apoptosis in Tongue Squamous Cell Carcinoma by Modulating miR-21/FOXG1 Pathway

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.17:463-469 ◽

2019 ◽

Vol 17 (4) ◽

pp. 463-469

Author(s):

Hou Deqiang ◽

Gao Yufeng ◽

Bai Ning ◽

Dong Yu

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Carcinoma Cells ◽

Tongue Squamous Cell Carcinoma ◽

Luciferase Assay ◽

Forkhead Box ◽

Proliferation And Apoptosis ◽

Mrna And Protein Expression ◽

Induced Apoptosis

Isoliquiritigenin is a flavonoid commonly found in liquorice and has been identified as a potent anti-tumor agent. The aim of this study was to investigate whether isoliquiritigenin regulates the proliferation and apoptosis of tongue squamous cell carcinoma cells by regulating forkhead box G1 expression via miR-21. MTT assay and flow cytometry were used to analyze cell proliferation and apoptosis, respectively. Quantitative real time polymerase chain reaction and western blotting were used to detect mRNA and protein expression levels, respectively. The relationship between miR-21 and forkhead box G1 was detected by dual luciferase assay. Isoliquiritigenin inhibited proliferation and induced apoptosis of tongue squamous cell carcinoma cells, and decreased miR-21 levels and promoted forkhead box G1 expression. Forkhead box G1 was then identified as a target of miR-21 and ISL could promote forkhead box G1 expression by inhibiting miR-21. Further analysis suggested that upregulation of miR-21 improved proliferation and suppressed apoptosis of tongue squamous cell carcinoma cells by inhibiting forkhead box G1 expression. Finally, our results revealed that isoliquiritigenin inhibited proliferation and induced apoptosis of tongue squamous cell carcinoma cells by regulating miR-21. Isoliquiritigenin might act as a novel therapeutic treatment for tongue squamous cell carcinoma cells through up-regulation of forkhead box G1 expression via inhibiting miR-21expression.

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Characterization of Healthy and Tumor Oral Cell Lines of Human Origin. The preliminary stage in the assessment of relevant chemical compounds with impact on dentistry

Revista de Chimie ◽

10.37358/rc.18.10.6647 ◽

2018 ◽

Vol 69 (10) ◽

pp. 2889-2894

Author(s):

Ion Virgil Corlan ◽

Adelina Cheveresan ◽

Delia Berceanu Vaduva ◽

Cristian Nica ◽

Alin Faur ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Cell Lines ◽

Squamous Cell ◽

Chemical Compounds ◽

Tongue Squamous Cell Carcinoma ◽

Gingival Fibroblasts ◽

Human Origin ◽

Preliminary Stage

The present study was aimed to evaluate the confluence percentage of three oral cell lines, namely primary gingival keratinocytes (PGK), primary gingival fibroblasts (HGF) and tongue squamous cell carcinoma (SCC-4). All cells have been monitored at different passages for 21 days. Evaluation of confluence percentage reveals the fact that primary gingival keratinocytes and tongue squamous cell carcinoma at small passages requires a period of about two weeks to reach a confluence of approximately 80% while for the gingival fibroblasts a period of about three times smaller is satisfactory.

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Prognostic Value of Lymph Node Density Compared to Pre-operative Platelet-to-Lymphocyte Ratio and Neutrophil-to-Lymphocyte Ratio in Patients with Tongue Squamous Cell Carcinoma

Journal of Maxillofacial and Oral Surgery ◽

10.1007/s12663-021-01524-1 ◽

2021 ◽

Author(s):

Mona Khazravi ◽

Mohammad Shirkhoda ◽

Hana Saffar ◽

Amirmohsen Jalaeefar

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Cell Carcinoma ◽

Squamous Cell ◽

Prognostic Value ◽

Tongue Squamous Cell Carcinoma ◽

Platelet To Lymphocyte Ratio ◽

Node Density ◽

Lymphocyte Ratio ◽

Lymph Node Density

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Prognostic value of MRI-measured tumor thickness in patients with tongue squamous cell carcinoma

Scientific Reports ◽

10.1038/s41598-021-90655-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ki-Sun Park ◽

Yangsean Choi ◽

Jiwoong Kim ◽

Kook-Jin Ahn ◽

Bum-soo Kim ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Prognostic Value ◽

Proportional Hazards ◽

Correlation Coefficients ◽

Cox Proportional Hazards ◽

Tumor Thickness ◽

Tongue Squamous Cell Carcinoma ◽

Depth Of Invasion

AbstractThis study aimed to assess the prognostic value of MRI-measured tumor thickness (MRI-TT) in patients with tongue squamous cell carcinoma (SCC). This single-center retrospective cohort study included 133 pathologically confirmed tongue SCC patients between January 2009 and October 2019. MRI measurements of tongue SCC were based on axial and coronal T2-weighted (T2WI) and contrast-enhanced T1-weighted (CE-T1WI) images. Two radiologists independently measured MRI-TT. Intraclass correlation coefficients (ICC) were calculated for inter-rater agreements. Spearman’s rank correlation between MRI-TT and pathologic depth of invasion (pDOI) was assessed. Cox proportional hazards analyses on recurrence-free (RFS) and overall survival (OS) were performed for MRI-TT and pDOI. Kaplan–Meier survival curves were plotted with log-rank tests. The intra- and inter-rater agreements of MRI-TT were excellent (ICC: 0.829–0.897, all P < 0.001). The correlation between MRI-TT and pDOI was good (Spearman’s correlation coefficients: 0.72–0.76, P < 0.001). MRI-TT were significantly greater than pDOI in all axial and coronal T2WI and CE-T1WI (P < 0.001). In multivariate Cox proportional hazards analysis, MRI-TT measured on axial CE-T1WI yielded a significant prognostic value for OS (hazards ratio 2.77; P = 0.034). MRI-TT demonstrated excellent intra- and inter-rater agreements as well as high correlation with pDOI. MRI-TT may serve as a prognostic predictor in patients with tongue SCC.

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