scholarly journals Apple Scion Cultivars Regulate the Rhizosphere Microbiota of Scion/rootstock Combinations

2020 ◽  
Author(s):  
Yi Wang ◽  
Xiaofen Chai ◽  
Xiaona Wang ◽  
Hui Li ◽  
Xuefeng Xu ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Plant Growth ◽  
Plant Traits ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Taxonomic Composition ◽  
Sugar Concentration ◽  
Composition And Structure ◽  
Plant Growth Promoting ◽  
Rhizosphere Bacterial Community

Abstract Grafting is a useful technique in the production of horticultural products. In addition to providing root system, rootstocks can increase stress tolerance of plants, influence scion growth and fruit yield, and harbor rich microbial community. But whether the scion modify plant growth, rootstock phenotypes and rhizosphere bacterial community has not been clearly investigated. Here, 14 different combinations of two scion cultivars grafted on 7 rootstock genotypes were used as research materials, we analyzed the plant traits, rhizosphere bacterial community, and potential functionalities across these plants in the same orchard. We found that apple scion cultivars influenced the tree high and trunk circumference, and the sugar concentration in root varied significantly between scion cultivars, especially fructose and sucrose. Apple scion cultivars was the largest source of variation in the rhizosphere bacterial diversity and taxonomic composition of grafted combinations. The dominant rhizosphere bacterial taxa were correlated with the root sugar concentration, especially sucrose. And the PICRUSt showed that rhizosphere bacteria contained fructose and sucrose metabolism and with plant growth-promoting traits. Additionally, the scion cultivar significantly affected the predicted metabolism of the rhizosphere-associated bacterial communities. Our results showed that apple scion varieties could regulate the composition and structure of rhizosphere bacterial community in different scion / rootstock combinations, which may be achieved by controlling soluble sugar content, especially sucrose in roots.

scholarly journals Accumulation of beneficial bacteria in the rhizosphere of maize (Zea mays L.) grown in a saline soil in responding to a consortium of plant growth promoting rhizobacteria

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Jieli Peng ◽  
Jia Ma ◽  
Xiaoyan Wei ◽  
Cuimian Zhang ◽  
Nan Jia ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Plant Growth ◽  
Saline Soil ◽  
Plant Growth Promoting Rhizobacteria ◽  
Salt Resistance ◽  
Beneficial Bacteria ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Rhizosphere Bacterial Community ◽  
Growth Promoting

Abstract Purpose Salt stress reduces plant growth and is now becoming one of the most important factors restricting the agricultural productivity. Inoculation of plant growth-promoting rhizobacteria (PGPR) has been shown to confer plant tolerance against abiotic stress, but the detailed mechanisms of how this occurs remain unclear and the application effects in different reports are unstable. In order to obtain a favorite effect of PGPR inoculation and improve our knowledge about the related mechanism, we performed this study to analyze the mechanism of a PGPR consortium on improving the salt resistance of crops. Methods A region-specific (Saline land around Bohai Sea in China) PGPR consortium was selected that contains three strains (Pseudomonas sp. P8, Peribacillus sp. P10, and Streptomyces sp. X52) isolated from rhizosphere of Sonchus brachyotus DC. grown in a saline soil. By inoculation tests, their plant growth-promoting (PGP) traits and ability to improve the salt resistance of maize were investigated and shifting in rhizosphere bacterial community of the inoculated plants was analyzed using the high-throughput sequencing technology. Results The three selected strains were salt tolerant, presented several growth promoting properties, and inhibited several phytopathogenic fungi. The inoculation of this consortium promoted the growth of maize plant and enriched the beneficial bacteria in rhizosphere of maize in a saline soil, including the nitrogen fixing bacteria Azotobacter, Sinorhizobium, and Devosia, and the nitrification bacteria Candidatus Nitrososphaera, and Nitrosovibrio. Conclusions The bacterial consortium P8/P10/X52 could improve plant growth in a saline soil by both their PGP traits and regulating the rhizosphere bacterial community. The findings provided novel information about how the PGPR helped the plants in the view of microbiome.

scholarly journals Bacillus amyloliquefaciens FH-1 significantly affects cucumber seedlings and the rhizosphere bacterial community but not soil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jingjing Wang ◽  
Song Xu ◽  
Rong Yang ◽  
Wei Zhao ◽  
Dan Zhu ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Soil Properties ◽  
Bacillus Amyloliquefaciens ◽  
Soil Conditions ◽  
Plant Growth Promoting Bacteria ◽  
Linear Discriminant ◽  
Cucumber Seedlings ◽  
Ecological Roles ◽  
Plant Growth Promoting ◽  
Rhizosphere Bacterial Community

AbstractPlant growth-promoting bacteria (PGPB) inoculants have been applied worldwide. However, the ecological roles of PGPB under different soil conditions are still not well understood. The present study aimed to explore the ecological roles of Bacillus amyloliquefaciens FH-1 (FH) on cucumber seedlings, rhizosphere soil properties, and the bacterial community in pot experiments. The results showed that FH had significant effects on cucumber seedlings and the rhizosphere bacterial community but not on soil properties. The FH promoted cucumber seedlings growth, reduced the rhizosphere bacterial diversity, increased Proteobacteria, and decreased Acidobacteria. Linear discriminant analysis (LDA) effect size (LEfSe) revealed that FH enriched two taxa (GKS2_174 and Nannocystaceae) and inhibited 18 taxa (mainly Acidobacteria, Actinobacteria, BRC1, Chloroflexi, Plantctomycetes, and Verrucomicrobia). Co-occurrence network analysis demonstrated that FH increased bacteria-bacteria interactions and that Bacillus (genus of FH) had few interactions with the enriched and inhibited taxa. This might indicate that FH does not directly affect the enriched and inhibited taxa. Correlation analysis results displayed that cucumber seedlings’ weight and height/length (except root length) were significantly correlated with the 18 inhibited taxa and the enriched taxa Nannocystaceae. It was speculated that FH might promote cucumber seedling growth by indirectly enriching Nannocystaceae and inhibiting some taxa from Acidobacteria, Actinobacteria, BRC1, Chloroflexi, Plantctomycetes, and Verrucomicrobia.

Effects of inoculation with plant growth-promoting rhizobacteria from the Brazilian Amazon on the bacterial community associated with maize in field

2022 ◽  
Vol 170 ◽  
pp. 104297
Author(s):  
Jessica Aparecida Ferrarezi ◽  
Paula de Almeida Carvalho-Estrada ◽  
Bruna Durante Batista ◽  
Rafael Martins Aniceto ◽  
Bruno Augusto Prohmann Tschoeke ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Brazilian Amazon ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Exogenous Melatonin Modulates the Physiological and Biochemical Mechanisms of Drought Tolerance in Tartary Buckwheat (Fagopyrum tataricum (L.) Gaertn)

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2828
Author(s):  
Md. Shakhawat Hossain ◽  
Jing Li ◽  
Ashim Sikdar ◽  
Mirza Hasanuzzaman ◽  
Ferdinand Uzizerimana ◽  
...  
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Growth And Yield ◽  
Tartary Buckwheat ◽  
Arid Zones ◽  
Enzymatic Antioxidants ◽  
Fagopyrum Tataricum ◽  
Exogenous Melatonin

Tartary buckwheat is one of the nutritious minor cereals and is grown in high-cold mountainous areas of arid and semi-arid zones where drought is a common phenomenon, potentially reducing the growth and yield. Melatonin, which is an amphiphilic low molecular weight compound, has been proven to exert significant effects in plants, under abiotic stresses, but its role in the Tartary buckwheat under drought stress remains unexplored. We evaluated the influence of melatonin supplementation on plant morphology and different physiological activities, to enhance tolerance to posed drought stress by scavenging reactive oxygen species (ROS) and alleviating lipid peroxidation. Drought stress decreased the plant growth and biomass production compared to the control. Drought also decreased Chl a, b, and the Fv/Fm ratio by 54%, 70%, and 8%, respectively, which was associated with the disorganized stomatal properties. Under drought stress, H2O2, O2•−, and malondialdehyde (MDA) contents increased by 2.30, 2.43, and 2.22-folds, respectively, which caused oxidative stress. In contrast, proline and soluble sugar content were increased by 84% and 39%, respectively. However, exogenous melatonin (100 µM) could improve plant growth by preventing ROS-induced oxidative damage by increasing photosynthesis, enzymatic antioxidants (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase), secondary metabolites like phenylalanine ammonialyase, phenolics, and flavonoids, total antioxidant scavenging (free radical DPPH scavenging), and maintaining relative water content and osmoregulation substances under water stress. Therefore, our study suggested that exogenous melatonin could accelerate drought resistance by enhancing photosynthesis and antioxidant defense in Tartary buckwheat plants.

scholarly journals Plant growth promotion of barley (Hordeum vulgare L.) by potassium solubilizing bacteria with multifarious plant growth promoting attributes

2021 ◽  
Vol 8 (sp1) ◽  
pp. 17-24
Author(s):  
Tanvir Kaur ◽  
Rubee Devi ◽  
Divjot Kour ◽  
Ashok Yadav ◽  
Ajar Nath Yadav
Keyword(s):  
Plant Growth ◽  
Environmental Sustainability ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Sugar Content ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Hordeum Vulgare L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Potassium (K) is the foremost macronutrients for growth of plant, soil health and fertility. The huge application of NPK chemical fertilizers negatively impacts the economy and is a threat to environmental sustainability. The rapid depletion of K mineral in soil is due to the application of agrochemicals agricultural fields for the production of crops in India. In present investigation, K-solubilizing microbes (KSM) were isolated and enumerated from cereal crops growing in Sirmour Himachal Pradesh. A total 125 bacteria were isolated and screened for K- solubilization on Aleksandrov agar plates and found that 31 bacterial strains exhibited K-solubilization. These 31 K-solubilizing strains of bacteria were additionally screened for other plant growth promoting (PGP) potential including solubilization of minerals, production of siderophores, ammonia, hydrogen cyanide and indole acetic acids. The performance of an efficient K-solubilizer was evaluated for plant growth promoting ability in pot assay under in vitro conditions. The strain EU-LWNA-25 positively influenced shoot length, fresh weight, carotenoids and total sugar content than the full dose, half dose and control. The strain enhancing physiological and growth parameters was identified by BLASTn analysis as Pseudomonas gessardii EU-LWNA-25. K-solubilizing plant growth promoting bacteria could be suitable bioinoculants for Rabi seasonal crops and overcomes the challenges of sustainable agriculture in K-deficient soil.

scholarly journals Diversity of Endophytic Bacteria Associated with Different Cultivars of Medicinal Plant Arctium Lappa L. and Their Potential for Host Plant Growth Promoting

2021 ◽  
Author(s):  
Jia-Qi Liu ◽  
Chun-Mei Zhang ◽  
Yuan Gong ◽  
Ming-Jie Xu ◽  
Ke Xing ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
High Throughput Sequencing ◽  
Promoting Effect ◽  
Pgp Traits ◽  
Arctium Lappa ◽  
Bacterial Phyla ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Purpose Arctium lappa L. is one of the medicinal and food homologous plants in China, which is rich in nutrients and medicinal ingredients. The use of plant growth promoting (PGP) endophytic bacteria is a useful alternative in agricultural production to reduce the use of chemical fertilizers. The aim of this study was to analysis the diversity of endophytic bacteria in different cultivars of A. lappa L. collected from two different geographical locations in China and evaluate PGP traits of the isolates and their potential PGP ability in greenhouse condition. Methods Endophytic bacterial community was investigated by culture-dependent and culture-independent methods. Isolates were screened and investigated for multiple PGP traits, and representative strains were inoculated host seedlings to evaluate the growth promoting effect. Results A total of 348 endophytic bacteria were obtained and they distributed into four phyla, 30 genera and 73 different species. In addition, high throughput sequencing revealed more abundant bacterial community, including 17 bacterial phyla, and 207 different known genera. A high proportion of PGP traits were detected, including production of indole acetic acid, siderophore, ammonia and phosphate solubilization. Four representative strains with multiple PGP traits of the most prevalent genera were further selected for host inoculation and growth promoting evaluation, and they significantly increase seedlings length, root length and fresh weight. Conclusion This study demonstrated that A. lappa L. harbors abundant endophytic bacteria, and plant genotype and geographical origin affect their composition. Moreover, some endophytic bacteria showed good potential for the development of microbial fertilizer in the future.

scholarly journals Arbuscular Mycorrhiza and Plant Growth-promoting Bacteria Alleviate Drought Stress in Walnut

HortScience ◽  
2019 ◽  
Vol 54 (6) ◽  
pp. 1087-1092 ◽  
Author(s):  
Azadeh Behrooz ◽  
Kourosh Vahdati ◽  
Farhad Rejali ◽  
Mahmoud Lotfi ◽  
Saadat Sarikhani ◽  
...  
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Soluble Sugar ◽  
Am Fungi ◽  
Total Phenolic ◽  
Plant Growth Promoting Bacteria ◽  
Negative Effects ◽  
Total Soluble Sugar ◽  
Peroxidase Enzyme ◽  
Plant Growth Promoting

Drought stress is one of the main constraints limiting worldwide crop production. Arbuscular mycorrhizae (AM) and plant growth-promoting bacteria (PGPB) such as Azotobacter chroococcum and Azospirillium lipofrum have been shown to alleviate drought stress effects. Therefore, the interaction effect of AM fungi [Glomus mosseae, G. etunicatum, and a mix of these (G. mix), and PGPB bacteria (Azotobacter chroococcum + Azospirillium lipofrum)] was investigated in 1-year-old walnut seedlings (cv. Chandler) under normal and drought stress conditions. Drought stress reduced growth (plant height, root length, number of leaves, and fresh weight) and leaf nutrient content (N, P, and Zn) significantly of walnut plants. In contrast, proline, total soluble sugar, starch peroxidase enzyme activity, and total phenolic content of walnut leaves increased under this stress. Application of fungi or bacteria, and especially their simultaneous use, alleviated the negative effects of drought stress on walnut seedlings. AM fungi and PGPB increased significantly the content of some metabolites, including total phenolic content, proline level, peroxidase activity, total soluble sugar, and starch content as well as peroxidase enzyme activity. This led to an increase in walnut plant growth under the drought stress condition. Among AM fungi, G. etunicatum was more effective in reducing drought stress symptoms than either G. mosseae or the G. mix of fungi. In conclusion, use of G. etunicatum, along with PGPB, can reduce negative effects of drought stress on walnut seedlings.

Deciphering the Mechanisms of Microbe Mediated Drought Stress Alleviation in Wheat

2021 ◽  
Author(s):  
DEVENDRA SINGH ◽  
Shobit Thapa ◽  
Jagriti Yadav ◽  
Dikchha SINGH ◽  
Hillol Chakdar ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Plant Growth ◽  
Sugar Content ◽  
Pcr Analysis ◽  
Stress Alleviation ◽  
Microbial Inoculation ◽  
Water Stress Condition ◽  
Plant Growth Promoting ◽  
Plant Growth Promoting Microbes

Abstract Drought stress adversely influences the crop plants. Herein, present research was designed to elucidate the role of plant growth promoting microbes for amelioration of water stress in wheat. A pot experiment was conducted for screening the microorganisms on the basis of plant growth, chlorophyll and proline content under water stress. Bacillus sp. BT3 and Klebsiella sp. HA9 were found more promising strains that positively influenced the plant growth, chlorophyll and proline status of seedlings under water stress condition. Further, Bacillus sp. BT-3 and Klebsiella sp. HA9 along with check strain (BioNPK) were used for elucidating their detailed effect on morphological, biochemical, physiological and molecular traits to mitigate drought stress in wheat. Microbial inoculation significantly enhanced plant growth, biomass, relative water content, chlorophyll content and root morphological parameters over the uninoculated water stressed (30% FC) control. Likewise, sugar content, protein content and antioxidant enzymes were also significantly enhanced due to microbial inoculation under water stress (30% FC). Microbial inoculation significantly decreased proline, glycine betaine, lipid peroxidation, peroxide and superoxide radicals in wheat over the uninoculated water stressed (30%FC) control. Quantitative real-time (qRT)- PCR analysis revealed that Bacillus sp. BT-3, Klebsiella sp. HA9 and BioNPK inoculation significantly upregulated stress responsive genes (DHN, DREB, L15 and TaABA-8OH) over the uninoculated water stressed (30% F.C.) control. The study reports the potential of Bacillus sp. BT3 and Klebsiella sp. HA9 along with BioNPK in water stress alleviation in wheat which could be recommended as effective biofertilizers.

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