scholarly journals A GC-MS-based Metabolomic Strategy to Investigate the Protective Effects of Mulberry Polysaccharide on CCl4-induced Acute Liver Injury in Mice

2021 ◽  
Author(s):  
Xiaofen Ma ◽  
Jian Yang ◽  
Subinur Baker ◽  
Zhen Li ◽  
Xin Li ◽  
...  
Keyword(s):  
Liver Injury ◽  
Acute Liver Injury ◽  
Gas Chromatography Mass Spectrometry ◽  
Protective Effects ◽  
Protective Mechanisms ◽  
Tissue Sections ◽  
Hematoxylin And Eosin ◽  
Endogenous Metabolites ◽  
Mulberry Tree ◽  
Southern Xinjiang

Abstract BackgroundMulberry (Morus alba) fruits of the woody mulberry tree (family: Moraceae Morus) is a type of mulberry fruit grown in the southern Xinjiang region,which polysaccharides have antioxidant and liver protective effects.This article further preliminary study on the protective effects of mulberry polysaccharide (MP) on liver.MethodsA detection kit was used to assess serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver malondialdehyde (MDA), superoxide dismutase (SOD) and other indicators. Liver tissue sections were stained with hematoxylin and eosin (H&E) and observed under a microscope. The entire endogenous metabolite profiling was acquired via metabolomics strategy using gas chromatography-mass spectrometry (GC-MS) to assess the underlying protective mechanisms of MPs. ResultsResults indicated that MPs exerted a hepatoprotective effect on acute liver injury by decreasing serum ALT and AST levels, hepatic MDA, and restored hepatic SOD glutathione peroxidase (GSH-Px) activities. A total of 33 possible endogenous metabolites associated with lipid, glucose, and energy metabolism including amino acids, sugars, and fatty acids, were found.ConclusionsThe results of the present study provide a reference for elucidating the protective mechanisms of MPs against acute liver injury.

scholarly journals Lemon Balm and Dandelion Leaf Extracts Synergistically Protect against Carbon Tetrachloride-Induced Acute Liver Injury in Mice

2021 ◽  
Vol 11 (1) ◽  
pp. 390
Author(s):  
Beom-Rak Choi ◽  
Il-Je Cho ◽  
Su-Jin Jung ◽  
Jae-Kwang Kim ◽  
Dae-Geon Lee ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Antioxidant Activities ◽  
Body Weight Gain ◽  
Histopathological Analysis ◽  
Related Factor ◽  
Mrna Levels ◽  
Protective Effects ◽  
Lemon Balm

Lemon balm and dandelion are commonly used medicinal herbs exhibiting numerous pharmacological activities that are beneficial for human health. In this study, we explored the protective effects of a 2:1 (w/w) mixture of lemon balm and dandelion extracts (MLD) on carbon tetrachloride (CCl4)-induced acute liver injury in mice. CCl4 (0.5 mL/kg; i.p.) injection inhibited body weight gain and increased relative liver weight. Pre-administration of MLD (50–200 mg/kg) for 7 days prevented these CCl4-mediated changes. In addition, histopathological analysis revealed that MLD synergistically alleviated CCl4-mediated hepatocyte degeneration and infiltration of inflammatory cells. MLD decreased serum aspartate aminotransferase and alanine transferase activities and reduced the number of liver cells that stained positive for cleaved caspase-3 and cleaved poly(ADP-ribose) polymerase, suggesting that MLD protects against CCl4-induced hepatic damage via the inhibition of apoptosis. Moreover, MLD attenuated CCl4-mediated lipid peroxidation and protein nitrosylation by restoring impaired hepatic nuclear factor erythroid 2-related factor 2 mRNA levels and its dependent antioxidant activities. Furthermore, MLD synergistically decreased mRNA and protein levels of tumor necrosis factor-α, interleukin-1β, and interleukin-6 in the liver. Together, these results suggest that MLD has potential for preventing acute liver injury by inhibiting apoptosis, oxidative stress, and inflammation.

scholarly journals Fucosterol Protects against Concanavalin A-Induced Acute Liver Injury: Focus on P38 MAPK/NF-κB Pathway Activity

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Wenhui Mo ◽  
Chengfen Wang ◽  
Jingjing Li ◽  
Kan Chen ◽  
Yujing Xia ◽  
...  
Keyword(s):  
Liver Injury ◽  
P38 Mapk ◽  
Concanavalin A ◽  
Molecular Mechanisms ◽  
Therapeutic Agent ◽  
Acute Liver Injury ◽  
Biological Activities ◽  
Hepatic Necrosis ◽  
Protective Effects ◽  
Eisenia Bicyclis

Objective. Fucosterol is derived from the brown alga Eisenia bicyclis and has various biological activities, including antioxidant, anticancer, and antidiabetic properties. The aim of this study was to investigate the protective effects of fucosterol pretreatment on Concanavalin A- (ConA-) induced acute liver injury in mice, and to understand its molecular mechanisms. Materials and Methods. Acute liver injury was induced in BALB/c mice by ConA (25 mg/kg), and fucosterol (dissolved in 2% DMSO) was orally administered daily at doses of 25, 50, and 100 mg/kg. The levels of hepatic necrosis, apoptosis, and autophagy associated with inflammatory cytokines were measured at 2, 8, and 24 h. Results. Fucosterol attenuated serum liver enzyme levels and hepatic necrosis and apoptosis induced by TNF-α, IL-6, and IL-1β. Fucosterol also inhibited apoptosis and autophagy by upregulating Bcl-2, which decreased levels of functional Bax and Beclin-1. Furthermore, reduced P38 MAPK and NF-κB signaling were accompanied by PPARγ activation. Conclusion. This study showed that fucosterol could alleviate acute liver injury induced by ConA by inhibiting P38 MAPK/PPARγ/NF-κB signaling. These findings highlight that fucosterol is a promising potential therapeutic agent for acute liver injury.

Protective effects of salecan against carbon tetrachloride-induced acute liver injury in mice

2011 ◽  
Vol 32 (10) ◽  
pp. 796-803 ◽  
Author(s):  
Peng Chen ◽  
Zhongqiu Wang ◽  
Liyan Zeng ◽  
Shiming Wang ◽  
Wei Dong ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Protective Effects

Corrigendum to “Protective effects of nicotinamide against acetaminophen-induced acute liver injury” [Int. Immunopharmacol. 14 (2012) 530–537]

2013 ◽  
Vol 16 (2) ◽  
pp. 341
Author(s):  
Youdan Shi ◽  
Li Zhang ◽  
Rong Jiang ◽  
Weiying Chen ◽  
Weiping Zheng ◽  
...  
Keyword(s):  
Liver Injury ◽  
Acute Liver Injury ◽  
Protective Effects

scholarly journals Resveratrol Pretreatment Ameliorates p53-Bax Axis and Augments the Survival Biomarker B-Cell Lymphoma 2 Modulated by Paracetamol Overdose in a Rat Model of Acute Liver Injury

Pharmacology ◽  
2019 ◽  
Vol 105 (1-2) ◽  
pp. 39-46 ◽  
Author(s):  
Suliman Al Humayed ◽  
Fahaid Al-Hashem ◽  
Mohamed A. Haidara ◽  
Abbas O. El Karib ◽  
Samaa S. Kamar ◽  
...  
Keyword(s):  
Single Dose ◽  
Liver Injury ◽  
B Cell ◽  
Nitrosative Stress ◽  
Acute Liver Injury ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Paracetamol Overdose ◽  
Protective Effects ◽  
Necrosis Factor Alpha

Background: The potential protective effects of resveratrol (RES) on the modulation of hepatic biomarkers of apoptosis and survival, p53-Bax axis, and B-cell lymphoma 2 (Bcl-2) in an animal model of paracetamol-induced acute liver injury have not been investigated before. Methods: The model group of rats received a single dose of paracetamol (2 g/kg, orally), whereas the protective group of rats were pretreated for 7 days with RES (30 mg/kg, i.p.) before they were given a single dose of paracetamol. All rats were then sacrificed 24-h post paracetamol ingestion. Results: Histology images showed that paracetamol overdose induced acute liver injury, which was substantially protected by RES. Paracetamol significantly (p < 0.05) modulated p53, apoptosis regulator Bax, Bcl-2, tumor necrosis factor-alpha, interleukin-6, inducible nitric oxide synthase, malondialdehyde, superoxide dismutase, glutathione peroxidase, alanine aminotransferase, and aspartate aminotransferase, which were significantly protected by RES. We further demonstrated a significant (p< 0.01) correlation between either p53 or Bcl-2 scoring and the levels of inflammatory, nitrosative stress, and liver injury biomarkers. Conclusion: We demonstrate a substantial protection by RES pretreatment against paracetamol-induced modulation of p53-Bax axis, Bcl-2, and other acute liver injury biomarkers in rats.

Protective effects of Flos lonicera extract on acute liver injury by dimethylnitrosamine-induced in rats

2010 ◽  
Vol 64 (3) ◽  
pp. 288-294 ◽  
Author(s):  
Yang Teng ◽  
Chang Hai Sun ◽  
Guangzhi Li ◽  
Guangchen Sun ◽  
Yui Nomachi ◽  
...  
Keyword(s):  
Liver Injury ◽  
Acute Liver Injury ◽  
Protective Effects

scholarly journals Protective Effect of Polydeoxyribonucleotide Against CCl4-Induced Acute Liver Injury in Mice

2020 ◽  
Vol 24 (Suppl 2) ◽  
pp. 88-95
Author(s):  
Seunghwan Lee ◽  
Kyu Yeoun Won ◽  
Sunhyung Joo
Keyword(s):  
Liver Injury ◽  
Intraperitoneal Injection ◽  
Acute Liver Injury ◽  
B Cell Lymphoma ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Tnf Α ◽  
Hematoxylin And Eosin ◽  
Urogenital Injury

Purpose: Polydeoxyribonucleotide (PDRN) is a substance known to suppress inflammation and accelerate wound healing. In this experiment, the effect of PDRN treatment on carbon tetrachloride (CCl<sub>4</sub>)-evoked acute liver injury (ALI) was investigated using mice.Methods: We analyzed the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and conducted hematoxylin and eosin staining in accompany with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining. Western blot analysis was also conducted to assess the expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, adenosine A<sub>2A</sub> receptor, Bcl-2-associated X protein (Bax), and B-cell lymphoma 2 (Bcl-2). The mice were received intraperitoneal injection of 10-mL/kg CCl<sub>4</sub>, 4 times, once every 2 days. The mice in the PDRN treatment groups received intraperitoneal injection of 200-μL distilled water comprising each concentration of PDRN for 7 days starting 1 day after first CCl<sub>4</sub> injection.Results: ALT and AST concentrations in the serum were reduced and TNF-α, IL-1β, and IL-6 expressions were decreased by PDRN injection in CCl<sub>4</sub>-evoked ALI mice. PDRN injection suppressed Bax versus Bcl-2 ratio and reduced the percentage of TUNE-positive cells in CCl<sub>4</sub>-evoked ALI mice. PDRN injection overexpressed adenosine A<sub>2A</sub> receptor in CCl<sub>4</sub>-evoked ALI mice.Conclusions: The therapeutic efficacy of PDRN also can be expected for CCl<sub>4</sub>-evoked acute urogenital injury in addition to ALI. The current research suggests that PDRN may be used for the therapeutic agent of CCl<sub>4</sub>-evoked ALI.

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