The Effect of eIF4E Antisense RNA Expression on Prostate Tumor Angiogenesis and Growth

1999 ◽  
Author(s):  
Briana J. Williams
Keyword(s):  
Tumor Angiogenesis ◽  
Antisense Rna ◽  
Prostate Tumor ◽  
Rna Expression

Permanent Inhibition of Angiotensinogen Synthesis by Antisense RNA Expression

Hypertension ◽  
1996 ◽  
Vol 27 (3) ◽  
pp. 508-513 ◽  
Author(s):  
Martina Schinke ◽  
Manfred Böhm ◽  
Giampiero Bricca ◽  
Detlev Ganten ◽  
Michael Bader
Keyword(s):  
Antisense Rna ◽  
Rna Expression

Novel Cellularly Active Inhibitor Regresses DDAH1 Induced Prostate Tumor Growth by Restraining Tumor Angiogenesis through Targeting DDAH1/ADMA/NOS Pathway

2019 ◽  
Vol 21 (4) ◽  
pp. 241-256 ◽  
Author(s):  
Karthik Reddy Kami Reddy ◽  
Chandrashekhar Dasari ◽  
Shalini Vandavasi ◽  
Sirisha Natani ◽  
Bhukya Supriya ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Tumor Angiogenesis ◽  
Prostate Tumor ◽  
Active Inhibitor

scholarly journals Characterization of Hypoxia induced gene 1: expression during rat Central Nervous System maturation and evidence of antisense RNA expression

2005 ◽  
Vol 49 (4) ◽  
pp. 431-436 ◽  
Author(s):  
Gabriela Bedo ◽  
Marcelo Vargas ◽  
Maria-Jose Ferreiro ◽  
Cora Chalar ◽  
Daniella Agrati
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Antisense Rna ◽  
Rna Expression

Effect of chitinase antisense RNA expression on disease susceptibility of Arabidopsis plants

1994 ◽  
Vol 25 (4) ◽  
pp. 587-596 ◽  
Author(s):  
Deborah A. Samac ◽  
Dilip M. Shah
Keyword(s):  
Antisense Rna ◽  
Disease Susceptibility ◽  
Rna Expression

scholarly journals New Architectures for Tet-On and Tet-Off Regulation in Staphylococcus aureus

2009 ◽  
Vol 76 (3) ◽  
pp. 680-687 ◽  
Author(s):  
Elena Stary ◽  
Rosmarie Gaupp ◽  
Sabrina Lechner ◽  
Martina Leibig ◽  
Evelyn Tichy ◽  
...  
Keyword(s):  
Gene Expression ◽  
Staphylococcus Aureus ◽  
Antisense Rna ◽  
Target Gene ◽  
Chromosomal Location ◽  
Constitutive Expression ◽  
Rna Expression ◽  
Posttranscriptional Gene Silencing ◽  
Gene Functions ◽  
Dose Dependent

ABSTRACT Inducible expression is a valuable approach for the elucidation of gene functions. Here, we present new configurations of the tetracycline-dependent gene regulation (tet) system for Staphylococcus aureus. To provide improved and expanded modes of control, strains and plasmids were constructed for the constitutive expression of tetR or a variant allele, rev-tetR r2. The encoded regulators respond differently to the effector anhydrotetracycline (ATc), which causes target gene expression to be induced with TetR or repressed with rev-TetR. To quantify and compare regulation mediated by episomal or chromosomal (rev-)tetR constructs, expression from a chromosomal Pxyl/tet-gfpmut2 fusion was measured. Chromosomally encoded TetR showed tight repression and allowed high levels of dose-dependent gene expression in response to ATc. Regulatory abilities were further verified using a strain in which a native S. aureus gene (zwf) was put under tet control in its native chromosomal location. Tight repression was reflected by transcript amounts, which were barely detectable under repressed conditions and high in ATc-treated cells. In reporter gene assays, this type of control, termed Tet-on, was more efficient than Tet-off regulation, in which addition of ATc causes downregulation of a target gene. The latter was achieved and quantified by direct rev-TetR control of P xyl/tet -gfpmut2. Additionally, TetR was used in trans to control the expression of antisense RNA for posttranscriptional gene silencing. Induction of antisense RNA expression of the fabI gene caused pronounced growth retardation lasting several hours. These results demonstrate the efficiency of the new tet systems and their flexible use for different purposes.

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