scholarly journals Analysis of the polymorphism of microsatellite loci in Picea abies (L.) H. Karst. and Picea obovatа Ledeb populations

2021 ◽  
Author(s):  
В.А. Волков ◽  
Г.В. Калько
Keyword(s):  
Molecular Markers ◽  
Picea Abies ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Geographic Origin ◽  
European Part ◽  
Picea Obovata ◽  
Siberian Spruce ◽  
Planting Material ◽  
European Part Of Russia

Процессы генезиса хвойных лесов интенсивно изучаются с использованием методов молекулярного маркирования. Микросателлитные локусы рассматриваются в качестве наиболее полиморфной и воспроизводимой системы кодоминантных молекулярных маркеров. В цели исследования входило установить возможность использования микросателлитных маркеров для определения видовых различий между елью европейской и сибирской (Picea abies и Picea obovatа), а также оценить степень генетической обособленности региональных популяций ели на территории Европейской части РФ. Исследование показало, что использование набора из двенадцати микросателлитных маркеров позволяет разделить выборку елей, собранных с европейской и азиатской частей страны, на два генетических кластера, соответствующих видам Picea abies и Picea obovatа. Проанализированные в исследовании микросателлитные локусы могут быть использованы для оценки генетического разнообразия и географического происхождения ели европейской и сибирской. The processes of genesis of coniferous forests are widely investigating using molecular markers. Many studies are based on the analysis of microsatellite loci, which are acknowledged as the most reproducible and polymorphic co-dominant molecular markers. This study is aimed to establish the possibility of using microsatellite markers to determine differentiation between Norway and Siberian spruce (Picea abies and Picea obovata). Another task of this research was to assess the degree of genetic isolation of regional spruce population in the territory of European part of the Russian Federation. This study shows that using a large number of microsatellite markers allows to divide the sample of spruce collected from European part of Russia and Siberia in two genetic clusters, corresponding to Picea abies and Picea obovata. The microsatellite loci analyzed in the study can be used to assess genetic diversity and geographic origin of spruce trees and determine the origin of wood and planting material of Norway and Siberian spruce.

Characterization and cross-species transferability of a novel set of microsatellites derived from root transcriptomes of Camellia oleifera

2019 ◽  
Vol 17 (04) ◽  
pp. 371-374
Author(s):  
Shuangcheng Wu ◽  
Hang Ye ◽  
Yuansong Chen ◽  
Jiemei Deng ◽  
Jiexia Su ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Microsatellite Markers ◽  
Molecular Genetics ◽  
Microsatellite Loci ◽  
Woody Plant ◽  
Edible Oils ◽  
The Novel ◽  
Camellia Oleifera ◽  
Nucleotide Repeat

AbstractCamellia oleifera is an important woody plant producing healthy edible oils. People need a large number of molecular markers, especially microsatellite, in breeding of C. oleifera. In this study, we sequenced the root transcriptomes of C. oleifera, and then designed a novel set of microsatellite markers based on the root-expressed genes. We assembled a total of 57,121 unigenes with a length of 42.63 Mb, which harboured 15,902 microsatellites. Among these microsatellites, di-nucleotide repeat motifs were the most abundant group (56.45%), then followed by tri- (25.20%), mono- (12.12%), hexa- (3.21%), penta- (2.18%) and quad-nucleotide ones (0.84%). In total, 6738 primer pairs were designed successfully to amplify the microsatellite loci. To test these microsatellite markers, 48 primer pairs were randomly selected and synthesized and validated in C. oleifera and its eight relatives. Up to 75% of the primer pairs amplified in C. oleifera and its relatives, and 62.5% displayed polymorphism. The transferability and diverse alleles across its eight relatives were detected for each polymorphic primer pair. The novel set of microsatellites derived from the root transcriptomes here provided a useful resource for future molecular genetics improvement of C. oleifera and its relatives.

Late Quaternary history of North Eurasian Norway spruce (Picea abies ) and Siberian spruce (Picea obovata ) inferred from macrofossils, pollen and cytoplasmic DNA variation

2015 ◽  
Vol 42 (8) ◽  
pp. 1431-1442 ◽  
Author(s):  
Mari Mette Tollefsrud ◽  
Małgorzata Latałowa ◽  
W. O. van der Knaap ◽  
Christian Brochmann ◽  
Christoph Sperisen
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Late Quaternary ◽  
Picea Obovata ◽  
Dna Variation ◽  
Siberian Spruce ◽  
Cytoplasmic Dna ◽  
History Of ◽  
Quaternary History

scholarly journals THE EFFECTIVENESS OF NATURAL GROWTH STIMULANTS IN THE CULTIVATION OF SEEDLINGS OF PICEA OBOVATA LEDEB.

2021 ◽  
pp. 329-333
Author(s):  
Tatiana Vladimirovna Khurshkainen ◽  
Svetlana Karlenovna Stetsenko ◽  
Elena Mikhailovna Andreeva ◽  
Gennady Grigorievich Terekhov ◽  
Alexander Vasilievich Kutchin
Keyword(s):  
Forest Nursery ◽  
Picea Obovata ◽  
Siberian Spruce ◽  
Stages Of Growth ◽  
Planting Material ◽  
Agricultural Plants ◽  
Forest Nurseries ◽  
Slow Growing ◽  
Effective Growth ◽  
Seedling Tree

Picea obovata Ledeb. is a slow-growing in the early stages of growth type of coniferous trees. The use of growth stimulants in the cultivation of spruce seedlings can accelerate the production of standard planting material. The paper presents the results of research of plant growth regulators influence on spruce seedlings within three years of cultivation in forest nursery conditions. Before sowing seeds of spruce soak in solutions of biological-active preparations Verva and Verva-spruce, obtaining from Abies and Picea wood greenery extracts. The plants growth regulator Verva from Abies needles (operating substance – triterpenic acids) is applied in plant growing at cultivation agricultural and commercial crops. Operating substance of a preparation Verva-spruce used for protection of agricultural plants from diseases, are phenolic compounds of Picea wood greenery possessing fungicidal and insecticidal activity. It is established that spruce seedlings processed before sowing by bio-preparation had higher parameters of growth (seedling height, diameter of seedling tree at root neck) in comparison with control plants within three years of cultivation in conditions of forest nursery. Three-year spruce seedlings which have been grown up with use of growth stimulators Verva and Verva-spruce exceeded control on 20–30% on height and on 19–20% on seedling tree thickness.  Bio-preparations Verva and Verva-spruce can be recommended for application in forest nurseries as effective growth stimulators at cultivation of Siberian spruce landing material.

The extent and meaning of hybridization and introgression between Siberian spruce (Picea obovata) and Norway spruce (Picea abies): cryptic refugia as stepping stones to the west?

2016 ◽  
Vol 25 (12) ◽  
pp. 2773-2789 ◽  
Author(s):  
Yoshiaki Tsuda ◽  
Jun Chen ◽  
Michael Stocks ◽  
Thomas Källman ◽  
Jørn Henrik Sønstebø ◽  
...  
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Stepping Stones ◽  
The West ◽  
Picea Obovata ◽  
Siberian Spruce ◽  
Hybridization And Introgression

Inter-year variation in selfing, background pollination, and paternal contribution in a Norway spruce clonal seed orchard

2014 ◽  
Vol 44 (7) ◽  
pp. 760-767 ◽  
Author(s):  
Monika Dering ◽  
Andrzej Misiorny ◽  
Władysław Chałupka
Keyword(s):  
Picea Abies ◽  
Microsatellite Markers ◽  
Norway Spruce ◽  
Pollen Dispersal ◽  
Geographic Origin ◽  
Seed Orchard ◽  
Seed Crop ◽  
Clonal Seed Orchard ◽  
Paternal Contribution ◽  
Nuclear Microsatellite

We aimed to investigate inter-year variability in mating system and paternity in an experimental Norway spruce (Picea abies (L.) Karst.) clonal seed orchard that was established with five geographically distinct populations. Using five nuclear microsatellite markers, we analysed 1396 progeny of five mother trees representing each of the populations in three successive mast-years. Selfing varied among mother trees, ranging from 0% to 18.3% with a mean of 13.9% in 1994, 10.1% in 2004, and 7.6% in 2006. Considerable background pollination was observed in each of the three studied mast-years and reached ca. 58%. The paternity assignment conducted showed an uneven male contribution to the seed crop, what was partly related to the number of ramets per paternal clone. All provenances contributed to the seed crop, but with different input, and two provenances dominated. Effective pollen dispersal was significantly determined by distance, with 72.2% of the seed crop resulting from crosses at a distance of up to 15 m. Our study indicated that despite considerable differences in the geographic origin of populations, inter-mating between clones from each population was possible, which generally agrees with the expectations related to the establishment of this seed orchard.

Causes of ecosystem transformations variability in lakes of the european part of Russia and Western Siberia (diatom analysis)

2019 ◽  
pp. 79-82
Author(s):  
Lev V. Razumovsky
Keyword(s):  
Western Siberia ◽  
Graphical Analysis ◽  
European Part ◽  
Analysis Method ◽  
Climatic Region ◽  
Diatom Analysis ◽  
Lake Ecosystems ◽  
Distinctive Features ◽  
European Part Of Russia

On the basis of author's graphical analysis method, the typification of lake ecosystems transformation scenarios depending on the size of lakes was carried out. It was confirmed that the type of transformation depends not only on size of the lake, but also on the landscape and climatic region in which it is located. The distinctive features of lake ecosystems transformation types in the European part of Russia and in Western Siberia were analyzed and compared.

Аquatic and helophytic vegetation of the Yamozero Lake (The Komi Republic)

2011 ◽  
pp. 101-116 ◽  
Author(s):  
B. Yu. Teteryuk
Keyword(s):  
Eastern Europe ◽  
Plant Communities ◽  
Rare Species ◽  
Komi Republic ◽  
European Part ◽  
Regional Level ◽  
North East ◽  
The North ◽  
European Part Of Russia ◽  
Floristic Classification

The results of a sintaxonomical study of plant communities of the Yamozero lake (the North-East of the European part of Russia) are presented. The diversity of the aquatic and helophytic vegetation of the Yamozero lake consists of 16 associations and 2 communities of 6 unions, 4 orders and 2 classes of the floristic classification: Potamogetonetea (7 associations, 2 communities), Phragmito-Magnocaricetea (9 associations). Many of described associations are widely distributed in the Central and the Eastern Europe. Some associations have the boundaries of their ranges. Some communities include 2 rare species of regional level: Isoetes setacea and Sagittaria natans.

Peronosporales of Skadovsky Zvenigorod Biological Station (Moscow Region)

2018 ◽  
Vol 52 (1) ◽  
pp. 91-100
Author(s):  
E. Yu. Blagoveshchenskaya
Keyword(s):  
Downy Mildew ◽  
European Part ◽  
Moscow Region ◽  
State University ◽  
Biological Station ◽  
Annotated List ◽  
European Part Of Russia ◽  
First Time ◽  
Moscow State University

The paper provides the results of seven-year study of downy mildew on Skadovsky Zvenigorod Biological Station of Moscow State University (ZBS MSU, Moscow Region). A total of 29 species of Peronosporales (Oomycota) were revealed during the study. An annotated list of species is presented, among them Peronospora anemones is recorded for the first time for Russia, P. chelidonii and P. stachydis are new for the European part of Russia, 8 species are new for the Moscow Region.

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