scholarly journals Anesthetic Storage of Recalcitrant Seed: Nitrous Oxide Prolongs Longevity of Lychee and Longan

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 597-599 ◽  
Author(s):  
Sharon Sowa ◽  
Eric E. Roos ◽  
Francis Zee

Seeds of the recalcitrant species lychee (Litchi chinénsis Sonn.) and longan (Dimocarpus longan Lour.) were stored near 100% relative humidity at 8 to 10C in air, 80% nitrous oxide (N2O) plus 20% oxygen, or 100% nitrous oxide. The combination of anesthetic and oxygen extended storage longevity of both species. Seeds stored in 100% N2O lost terminability at the same rate as those stored in air. Lychee seeds retained 92% of initial germination after 12 weeks under 80% N2O/20% O2, while those under air retained only 44%. Longan seeds failed to germinate after 7 weeks under air, yet retained 70% of their initial germination under 809” N2O/20% O2. The combination of anesthetic and oxygen atmospheres could provide a new approach to recalcitrant seed storage.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1084a-1084
Author(s):  
Sharon Sowa ◽  
Eric E. Roos ◽  
Francis Zee

Seeds of the recalcitrant species Litch i chinénis and Euphoria longan were stored in humid conditions at 8-10C under three different atmospheres: air, 80% nitrous oxide (N20)/20 % oxygen, and 100% nitrous oxide. The combination of anesthetic and oxygen extended storage longevity of both species. Oxygen was required for maintenance of viability; seeds stored under 100% N20 lost germinability at the most rapid rate. Lychee seeds retained 92% of control germination after 12 weeks under 80% N20/20% 02, while those under air lost 56% viability. Longan seeds lost all viability after 7 weeks under air, yet retained 70% of their control germination under 80% N20/20% 02. The combination of anesthetic and oxygen atmospheres could provide a new approach to recalcitrant seed storage.


Author(s):  
Canh

This work presents the fabrication of the granular humidity indicator for the monitor of the relative moisture in the outdoor environment or in the enclosed areas such as bags, containers, enclosed rooms, boxes which are used for the storage of the weapons and military equipment, food preservation, seed storage in agriculture, controlling fermentation process as well as the plants growing process, etc. Moisture indicator was fabricated from cobalt chloride, desiccant, color-stabilizing agents, and silica gel particles. The obtained indicator can measure the relative humidity in the range of 30 to 70 based on the color changing from the indicator.


2014 ◽  
Vol 7 (5) ◽  
pp. 1201-1211 ◽  
Author(s):  
F. Navas-Guzmán ◽  
J. Fernández-Gálvez ◽  
M. J. Granados-Muñoz ◽  
J. L. Guerrero-Rascado ◽  
J. A. Bravo-Aranda ◽  
...  

Abstract. In this paper, we outline an iterative method to calibrate the water vapour mixing ratio profiles retrieved from Raman lidar measurements. Simultaneous and co-located radiosonde data are used for this purpose and the calibration results obtained during a radiosonde campaign in summer and autumn 2011 are presented. The water vapour profiles measured during night-time by the Raman lidar and radiosondes are compared and the differences between the methodologies are discussed. Then, a new approach to obtain relative humidity profiles by combination of simultaneous profiles of temperature (retrieved from a microwave radiometer) and water vapour mixing ratio (from a Raman lidar) is addressed. In the last part of this work, a statistical analysis of water vapour mixing ratio and relative humidity profiles obtained during 1 year of simultaneous measurements is presented.


2000 ◽  
Vol 10 (2) ◽  
pp. 183-187 ◽  
Author(s):  
P. Danthu ◽  
A. Guèye ◽  
A. Boye ◽  
D. Bauwens ◽  
A. Sarr

AbstractSeeds of four African tree species from the Sahelian zone (Boscia senegalensis) and the Sudanian zone (Butyrospermum parkii, Cordyla pinnata and Saba senegalensis) lose viability after moisture contents drop below 22 and 30%, depending on the species. Seed longevity in wet and airtight storage does not exceed a few months. Temperatures close to zero elicit symptoms of chilling injury leading to rapid seed death. The optimum storage temperature is 15°C. These results allow the four species to be classified as recalcitrant seed species. Boscia senegalensis is therefore an exception in arid zones where most species have orthodox seeds.


2021 ◽  
Vol 27 (3) ◽  
pp. 162-166
Author(s):  
Abdul Azeez Hussain ◽  
◽  
Ramachandra Kurup Rajvikraman ◽  

Detailed study on seed storage and germination trailed in Knema attenuata (Wall. ex Hook. f. & Thomson) Warb.– the IUCN Red Listed ‘least concern’ medicinal tree species revealed that seeds were of recalcitrant nature. Viability of the seeds could be maintained for a longer period of up to 6 months with 47% Moisture content (mc) when kept in closed polycarbonate bottles at seed bank condition [20±20C Temp. and 40% Relative Humidity (RH)]. The 55% seed germination under normal climatic condition could be enhanced to a much higher percentage (75±5) inside the mist house chamber (34±30C Temp. and 70-80% RH).


2018 ◽  
Vol 40 (3) ◽  
pp. 221-236 ◽  
Author(s):  
Claudio José Barbedo

ABSTRACT: Water is essential, irreplaceable, and indispensable for any kind of carbon-based-life metabolic activity. Water-dependent living beings are the expected pattern in nature. However, some organisms can survive for some time at a minimum water content, such as seeds of some species (orthodox seeds). Nevertheless, the expected standard life behavior is found in seeds of another group of species, the so-called recalcitrant seeds, which are sensitive to desiccation. A huge range of different behaviors can be found between these two groups, leading authors to consider that orthodoxy and recalcitrance is not an all-or-nothing situation. Notwithstanding, we are still too far from understanding the differences and similarities between all these kinds of seeds and this has been a serious barrier to the development of plant conservation technologies. A new approach to understanding the differences between these seeds is presented here based on seed maturation, environmental influences, and evolution. From this point of view, all kinds of seed behavior are contemplated and, consequently, some new perspectives are considered for the recalcitrant seed conservation technology, the most intensely desired technology nowadays in this area.


2013 ◽  
Vol 6 (6) ◽  
pp. 10481-10510
Author(s):  
F. Navas-Guzmán ◽  
J. Fernández-Gálvez ◽  
M. J. Granados-Muñoz ◽  
J. L. Guerrero-Rascado ◽  
J. A. Bravo-Aranda ◽  
...  

Abstract. In this paper, we outline an iterative method to calibrate the water vapour mixing ratio profiles retrieved from Raman lidar measurements. Simultaneous and co-located radiosonde data are used for this purpose and the calibration results obtained during a radiosonde campaign performed in Summer and Autumn 2011 are presented. The water vapour profiles measured during nighttime by the Raman lidar and radiosondes are compared and the differences between the methodologies are discussed. Moreover, a new approach to obtain relative humidity profiles by combination of simultaneous profiles of temperature (retrieved from a microwave radiometer) and water vapour mixing ratio (from a Raman lidar) is addressed. In the last part of this work, a statistical analysis of water vapour mixing ratio and relative humidity profiles obtained during one year of simultaneous measurements is presented.


2008 ◽  
Vol 35 (2) ◽  
pp. 108-115 ◽  
Author(s):  
B. R. Morton ◽  
B. L. Tillman ◽  
D. W. Gorbet ◽  
K. J. Boote

Abstract Late-maturing peanut cultivars including DP-1, C-99R, Hull, Georgia-01R, and Florida MDR-98 (Arachis hypogaea L.) have improved resistance to late leaf spot, white mold, and tomato spotted wilt virus. The improved disease resistances offer potential cost savings for farmers. However, poor field emergence resulted in unacceptable plant stands and caused commercial seed companies to stop producing Florida MDR-98, DP-1, and Hull even though germination tests usually showed acceptable germination. Reduced field emergence seldom occurs when seed of these cultivars is grown, harvested, and stored in small batches in research storage facilities. To determine the effect of storage environment, pods of four cultivars were stored in four environments and the seeds were subjected to standard germination tests and immediately followed by field emergence evaluations. Cultivar affected both field emergence and germination. Storage environment affected field emergence, and to a lesser extent, germination (P = 0.0847). Peanut pods stored in a traditional peanut warehouse experienced elevated temperature and relative humidity compared to seeds stored in climate controlled facilities and those seed had reduced field emergence. There was a cultivar by storage environment interaction. Field emergence was maintained when seed was stored where temperature was below 16 C and relative humidity (RH) was less than 70%. This work suggests that seed vigor of the cultivars like DP-1 and Hull deteriorates faster than that of other cultivars when stored in commercial warehouses even though germination tests indicate that the seed should produce satisfactory plant stands.


2019 ◽  
Vol 29 (2) ◽  
pp. 199-204
Author(s):  
Scott B. Lukas ◽  
Joseph DeFrank ◽  
Orville C. Baldos ◽  
Ruijun Qin

Seed dormancy is an evolutionary adaptation for increasing seedling survival by delaying germination and is found in many families of seed plants. Although dormancy is ecologically important, it becomes problematic during agronomic production and restoration. Torrid panicgrass (Panicum torridum) is a native Hawaiian annual grass that has been identified as a re-vegetation candidate for seasonally dry areas. Torrid panicgrass seed appears to possess a nondeep to intermediate physiological dormancy. This research aimed to characterize dormancy relief parameters by 1) evaluating exogenous hormonal, reactive oxygen intermediates, and simulated combustion product treatments; and 2) determining optimized storage conditions of relative humidity (RH) and temperature over a 10-month duration. Results indicate that all exogenous chemical treatments tested were not effective at relieving the dormancy present in torrid panicgrass. Optimal storage conditions to relieve dormancy were found with seeds equilibrated to 12% RH, stored at 30 °C for a period of 8 months resulting in 55% germination. Maintenance of viability for long-term storage up to 10 months was best achieved with seeds stored at 12% RH at 10, 20, or 30 °C.


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