scholarly journals Torrid Panicgrass Dormancy Relief Through Exogenous Chemical Treatments and Seed Storage Optimization of Temperature and Relative Humidity Parameters

2019 ◽  
Vol 29 (2) ◽  
pp. 199-204
Author(s):  
Scott B. Lukas ◽  
Joseph DeFrank ◽  
Orville C. Baldos ◽  
Ruijun Qin
Keyword(s):  
Relative Humidity ◽  
Seedling Survival ◽  
Seed Storage ◽  
Storage Conditions ◽  
Oxygen Intermediates ◽  
Seasonally Dry ◽  
Chemical Treatments ◽  
Long Term Storage ◽  
Physiological Dormancy

Seed dormancy is an evolutionary adaptation for increasing seedling survival by delaying germination and is found in many families of seed plants. Although dormancy is ecologically important, it becomes problematic during agronomic production and restoration. Torrid panicgrass (Panicum torridum) is a native Hawaiian annual grass that has been identified as a re-vegetation candidate for seasonally dry areas. Torrid panicgrass seed appears to possess a nondeep to intermediate physiological dormancy. This research aimed to characterize dormancy relief parameters by 1) evaluating exogenous hormonal, reactive oxygen intermediates, and simulated combustion product treatments; and 2) determining optimized storage conditions of relative humidity (RH) and temperature over a 10-month duration. Results indicate that all exogenous chemical treatments tested were not effective at relieving the dormancy present in torrid panicgrass. Optimal storage conditions to relieve dormancy were found with seeds equilibrated to 12% RH, stored at 30 °C for a period of 8 months resulting in 55% germination. Maintenance of viability for long-term storage up to 10 months was best achieved with seeds stored at 12% RH at 10, 20, or 30 °C.

scholarly journals Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Shidong Yue ◽  
Yu Zhang ◽  
Yi Zhou ◽  
Shaochun Xu ◽  
Shuai Xu ◽  
...  
Keyword(s):  
Seed Storage ◽  
Storage Conditions ◽  
Seed Vigor ◽  
Ex Situ ◽  
Zostera Japonica ◽  
Storage Method ◽  
Seagrass Meadows ◽  
Long Term Storage ◽  
Term Storage

Abstract Background Seagrass meadows are recognized as critical and among the most vulnerable habitats on the planet. The alarming rates of decline in seagrass meadows have attracted the attention globally. There is an urgent need to develop techniques to restore and preserve these vital coastal ecosystems. So far little work has been done to develop effective long-term storage method for seagrass seeds. The seagrass Zostera japonica Asch. & Graebn is an endangered species in its native range. Here we utilized combinations of different storage times, salinities, and temperature to determine the most appropriate conditions for optimal seed storage. Results Zostera japonica seeds were strongly desiccation sensitive, with a complete loss of viability after 24 h of desiccation. Therefore, long periods of exposure to air should be avoided to minimize seed mortality. In addition, Z. japonica seeds could not endure freezing conditions such as – 5 °C. However, our results indicated that reduced storage temperature to 0 °C could effectively prolong the duration of dormancy of Z. japonica seeds. Seeds stored at 0 °C under a salinity of 40–60 psu showed relatively low seed loss, high seed vigor and fast seed germination, suggesting these to be optimal seed storage conditions. For example, after storage for 540 days (ca. 600 days since the seed collection from reproductive shoots in early October, 2016) at 0 °C under a salinity of 50 psu, seeds still had a considerable vigor, i.e. 57.8 ± 16.8%. Conclusion Our experiments demonstrated that seeds stored at 0 °C under a salinity of 40–60 psu could effectively prolong the duration of dormancy of Z. japonica seeds. The proposed technique is a simple and effective long-term storage method for Z. japonica seeds, which can then be used to aid future conservation, restoration and management of these sensitive and ecologically important habitat formers. The findings may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.

scholarly journals The Longevity of Crop Seeds Stored Under Long-term Condition in the National Gene Bank of Bulgaria

2016 ◽  
Vol 62 (3) ◽  
pp. 90-100 ◽  
Author(s):  
Gergana Desheva
Keyword(s):  
Seed Viability ◽  
Seed Storage ◽  
Storage Conditions ◽  
Gene Bank ◽  
Common Vetch ◽  
Orchard Grass ◽  
Long Term Storage ◽  
Plant Families ◽  
Brome Grass

Abstract Seed accessions from 7 plant families and 28 species stored for above 20 years in the National gene bank of Bulgaria were evaluated. All seed accessions were maintained as base collection under long-term storage conditions with low moisture contents (5±2%) in hermetically closed containers at −18°C. On the basis of experimental data, the seed storage characters σ (standard deviation of seed death in storage), P50% (the time for viability to fall to 50%) and P10% (the time for viability reduction of 10%) were determined allowing the prediction of seed storage life and the regeneration needs. The results showed significant differences in loss of seed viability among species and within the species. After 20–24 years of storage, eleven crops showed minimal viability decline under 5% as compared to the initial viability (oats, barley, maize, bread wheat, durum wheat, smooth brome grass, faba bean, chickpea, sunflower, cucumber and pepper). For the same storage time, another group of crops (sorghum, triticale, orchard grass, tall fescue, common vetch, grass pea, lentil, common bean, rapeseed, tobacco, flax, cabbage and tomatoes) presented 5–10% reduction of seed viability. More significant changes in seed viability – above 10% – were detected for peanuts, lettuce, soybean and rye. The σ values varied from 20.41 years (Arachis hypogaea L.) to 500 years (for Avena sativa L. and Triticum aestivum L). There was wide variation across species, both in time taken for the viability to fall to 50% and in time taken for the seed viability reduction of 10%. The study illustrates the positive effect of both seed storability early monitoring and prediction of regeneration needs as a tool for limiting undesired losses.

scholarly journals Does Storage under Gene Bank Conditions Affect Seed Germination and Seedling Growth? The Case of Senecio morisii (Asteraceae), a Vascular Plant Exclusive to Sardinian Water Meadows

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 581 ◽  
Author(s):  
Alba Cuena-Lombraña ◽  
Martina Sanna ◽  
Marco Porceddu ◽  
Gianluigi Bacchetta
Keyword(s):  
Seedling Growth ◽  
Seedling Survival ◽  
Vascular Plant ◽  
Seed Viability ◽  
Storage Conditions ◽  
Growth And Survival ◽  
Survival Percentage ◽  
Long Term Storage ◽  
Term Storage

Understanding seed viability under long-term storage conditions provides basic and useful information to investigate the effectiveness of seed banking. Besides the germination success, seedling establishment is also an important requirement, and a decisive step to ensure plant propagation. We used comparative data of germination, seedling growth, and survival percentage between fresh and 10-years-stored seeds of Senecio morisii, a narrow endemic and vulnerable species of Sardinia (Italy), in order to evaluate if differences exist in these traits between fresh and 10-years-stored seeds. Stored seeds showed higher germination percentages than fresh ones, whereas seedling growth and survival did not present significant differences between them, except for seedling growth in plants produced from seeds germinated at 25 °C. This study allowed us to assess if seeds of S. morisii were able to germinate under controlled conditions, and if they maintained their viability and germination capacity for at least 10 years of long-term storage in the seed bank. In addition, the high seedling survival detected in both fresh and stored seeds suggests that stored seeds of S. morisii can be used to support reinforcement or reintroduction actions when fresh materials are not available.

scholarly journals Co-encapsulation of vegetable oils with phenolic antioxidants and evaluation of their oxidative stability under long-term storage conditions

LWT ◽  
2021 ◽  
Vol 142 ◽  
pp. 111033
Author(s):  
Lorine Le Priol ◽  
Justine Gmur ◽  
Aurélien Dagmey ◽  
Sandrine Morandat ◽  
Karim El Kirat ◽  
...  
Keyword(s):  
Oxidative Stability ◽  
Vegetable Oils ◽  
Storage Conditions ◽  
Phenolic Antioxidants ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Effects of Long-Term Storage on the Detection of Proteins, DNA, and mRNA in Tissue Microarray Slides

2011 ◽  
Vol 59 (12) ◽  
pp. 1113-1121 ◽  
Author(s):  
Christina Karlsson ◽  
Mats G. Karlsson
Keyword(s):  
Gene Copy Number ◽  
Tissue Microarrays ◽  
Storage Conditions ◽  
Gene Copy ◽  
Histological Techniques ◽  
Long Term Storage ◽  
Term Storage ◽  
Formalin Fixed

Storage of tissue slides has been claimed to induce dramatically reduced antigen detection particularly for immunohistochemistry (IHC). With tissue microarrays, the necessity to serially cut blocks in order to obtain as much material as possible is obvious. The presumed adverse effect of storage might hamper such an approach. The authors designed an experimental setting consisting of four different storage conditions with storage time of tissue slides of up to 1 year. Detection of proteins, DNA, and mRNA was performed using IHC and in situ hybridization techniques. Slight but significant changes in IHC occurred over time. The most important factor is the primary antibody used: four showed no significant changes, whereas limited decreases in 8 antibodies could be detected by image analysis. Whether the antigen was nuclear or cytoplasmic/membranous did not matter. No major differences between different storage conditions could be shown, but storage at 4C was overall the best procedure. Furthermore, gene copy number aberrations, chromosomal translocations, and the presence of mRNA could be detected on slides stored up to 1 year. In conclusion, in tissues optimally formalin fixed and using modern histological techniques, only minute changes in tissue antigenicity are induced by long-term storage.

scholarly journals Is adipose tissue suitable for detection of (synthetic) cannabinoids? A comparative study analyzing antemortem and postmortem specimens following pulmonary administration of JWH-210, RCS-4, as well as ∆9-tetrahydrocannabinol to pigs

2020 ◽  
Vol 94 (10) ◽  
pp. 3421-3431
Author(s):  
Nadine Schaefer ◽  
Frederike Nordmeier ◽  
Ann-Katrin Kröll ◽  
Christina Körbel ◽  
Matthias W. Laschke ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Synthetic Cannabinoids ◽  
Standard Addition ◽  
Storage Conditions ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Concentration Changes ◽  
Term Storage ◽  
Plateau Level

Abstract Examining fatal poisonings, chronic exposure may be reflected by the concentration in tissues known for long-term storage of drugs. Δ9-tetrahydrocannabinol (THC) persists in adipose tissue (AT), but sparse data on synthetic cannabinoids (SC) are available. Thus, a controlled pig study evaluating antemortem (AM) disposition and postmortem (PM) concentration changes of the SC 4-ethylnaphthalene-1-yl-(1-pentylindole-3-yl)methanone (JWH-210) and 2-(4-methoxyphenyl)-1-(1-pentyl-indole-3-yl)methanone (RCS-4) as well as THC in AT was performed. The drugs were administered pulmonarily (200 µg/kg body weight) to twelve pigs. Subcutaneous (s.c.) AT specimens were collected after 15 and 30 min and then hourly up to 8 h. At the end, pigs were sacrificed and s.c., perirenal, and dorsal AT specimens were collected. The carcasses were stored at room temperature (RT; n = 6) or 4 °C (n = 6) and specimens were collected after 24, 48, and 72 h. After homogenization in acetonitrile and standard addition, LC–MS/MS was performed. Maximum concentrations were reached 0.5–2 h after administration amounting to 21 ± 13 ng/g (JWH-210), 24 ± 13 ng/g (RCS-4), and 22 ± 20 ng/g (THC) and stayed at a plateau level. Regarding the metabolites, very low concentrations of N-hydroxypentyl-RCS-4 (HO-RCS-4) were detected from 0.5 to 8 h. PM concentrations of parent compounds did not change significantly (p > 0.05) over time under both storage conditions. Concentrations of HO-RCS-4 significantly (p < 0.05) increased in perirenal AT during storage at RT. These results suggest a rapid distribution and persistence in s.c. AT. Furthermore, AT might be resistant to PM redistribution of parent compounds. However, significant PM increases of metabolite concentrations might be considered in perirenal AT.

Effects of drying temperature and long-term storage conditions on black rice phenolic compounds

2019 ◽  
Vol 287 ◽  
pp. 197-204 ◽  
Author(s):  
Gustavo Heinrich Lang ◽  
Igor da Silva Lindemann ◽  
Cristiano Dietrich Ferreira ◽  
Jessica Fernanda Hoffmann ◽  
Nathan Levien Vanier ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Storage Conditions ◽  
Black Rice ◽  
Drying Temperature ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Personal medicines storage in New Zealand

2013 ◽  
Vol 5 (2) ◽  
pp. 146 ◽  
Author(s):  
Campbell Hewson ◽  
Chong Chi Shen ◽  
Clare Strachan ◽  
Pauline Norris
Keyword(s):  
New Zealand ◽  
Relative Humidity ◽  
Storage Conditions ◽  
Child Safety ◽  
Extreme Temperatures ◽  
Freezing And Thawing ◽  
Data Logger ◽  
Drug Storage ◽  
Storage Methods

INTRODUCTION: Poor storage of medicines can reduce their efficacy, yet little is known about how people store medicines in their homes and elsewhere, why these locations are chosen, and whether the conditions are suitable for medicines storage. AIM: To investigate where medicines are commonly stored in New Zealand households, why, and the typical conditions – temperature and relative humidity – in those places of storage. METHODS: Data from a large qualitative study on the meanings of medicines were analysed to explore where people store medicines in their households, and why. A data logger was used to log temperature and relative humidity in common medicine storage places, such as homes and cars. RESULTS: Kitchens and bathrooms were the most commonly reported storage places, with people influenced by convenience, desire to remember to take medicines, and child safety when deciding where to store medicines. High temperatures and humidity were found in kitchens and bathrooms, extreme temperatures in a car and a backpack, and extremely low temperatures in checked-in luggage on a plane. DISCUSSION: Temperature- and humidity-sensitive medicines should not be stored long-term in common storage locations, such as kitchens and bathrooms. Conditions in these places may not comply with the recommended storage conditions given by the manufacturer. Furthermore, medicines should not be left in backpacks or cars, especially if the vehicle is in the sun. Medicines that may degrade upon freezing and thawing – such as protein-containing medicines, emulsions, suspensions and some solutions – should not be stored in the cargo hold of a plane. KEYWORDS: Drug storage; humidity; New Zealand; temperature

Analysis of seed-bank data confirms suitability of international seed-storage standards for the Australian flora

2007 ◽  
Vol 55 (1) ◽  
pp. 18 ◽  
Author(s):  
Andrew D. Crawford ◽  
Kathryn J. Steadman ◽  
Julie A. Plummer ◽  
Anne Cochrane ◽  
Robin J. Probert
Keyword(s):  
Western Australia ◽  
Seed Bank ◽  
Seed Storage ◽  
Storage Conditions ◽  
International Standards ◽  
Australian Flora ◽  
Western Australian

The suitability of applying international standards for long-term seed storage to the Australian flora was examined by reviewing seed-storage data from the Western Australian Threatened Flora Seed Centre. The 375 collections examined represented 176 taxa from 44 genera and 16 families. A high proportion of collections, representative of some of the most common genera in Australia, maintained viability in the short (<5 years) and medium (5–12 years) term. Declines in germination were evident for a small number of collections, representing 10 taxa, stored for 5–12 years. However, many of the declines were collection-specific and other collections of the same taxon did not decline. Five taxa showing germination declines were represented by a single collection so it was not possible to determine whether the germination decline was directly related to the taxa, storage conditions or to other factors. Only the closely related Stylidium coroniforme and S. amabile exhibited germination declines in all collections, but cut tests of seeds remaining from germination testing indicated that viability of the collections had not declined, just the proportion to germinate; for these species a change in germination conditions is a more likely explanation for the reduction in germination. The results illustrate the successful application of these seed-storage standards to threatened flora in Western Australia and highlight their suitability for the Australian flora.

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