scholarly journals Characterizing Isozymes of Spanish Cherimoya Cultivars

HortScience ◽  
1993 ◽  
Vol 28 (8) ◽  
pp. 845-847 ◽  
Author(s):  
L. Pascual ◽  
F. Perfectti ◽  
M. Gutierrez ◽  
A.M. Vargas
Keyword(s):  
Cluster Analysis ◽  
Superoxide Dismutase ◽  
Acid Phosphatase ◽  
Malic Enzyme ◽  
Leucine Aminopeptidase ◽  
Shikimate Dehydrogenase ◽  
Banding Patterns ◽  
Enzyme Systems ◽  
Annona Cherimola ◽  
Glutamate Oxalacetate Transaminase

Isozymes have been used as genetic markers to characterize seven Spanish cherimoya (Annona cherimola Mill.) cultivars. Fifteen enzyme systems were analyzed. Ten varied [aconitase (ACO, EC 4.2.1.3), alcohol dehydrogenase (ADH, EC 1.1.1.1), glutamate oxalacetate transaminase (GOT, EC 2.6.1.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), leucine aminopeptidase (LAP, EC 3.4.11.1), malate dehydrogenase (MDH, EC 1.1.1.37), phosphoglucose isomerase (PGI, EC 5.3.1.9), phosphoghtcomutase (PGM, EC 2.7.5.1), shikimate dehydrogenase (SKDH, EC 1.1.1.25), and triose phosphate isomerase (TPI, EC 5.3.1.1)] and five did not [acid phosphatase (ACPH, EC 3.1.3.2), diaphorase (DIA, EC 1.6.4.3), malic enzyme (ME, EC 1.1.1.40), 6-phosphogluconic dehydrogenase (6PGDH, EC 1.1.1.44), and superoxide dismutase (SOD, EC 1.15.1.1)]. Two cultivars, Campa and Campa Mejorada, had identical banding patterns for all enzymes tested. All others were identified as distinct cultivars because of isozyme differences. The identical isozyme profiles of `Campa' and `Campa Mejorada' probably indicate that they are the same cultivar. A cluster analysis of isozyme profiles showed that Spanish cultivars were clearly different from Californian cultivars.

INHERITANCE OF ALLOZYME VARIANTS IN COASTAL DOUGLAS-FIR (PSEUDOTSUGA MENZIESII VAR. MENZIESII)

1982 ◽  
Vol 24 (3) ◽  
pp. 325-335 ◽  
Author(s):  
Y. A. El-Kassaby ◽  
F. C. Yeh ◽  
O. Sziklai
Keyword(s):  
Superoxide Dismutase ◽  
Acid Phosphatase ◽  
Pseudotsuga Menziesii ◽  
Malic Enzyme ◽  
Quaternary Structure ◽  
Allelic Variation ◽  
Enzyme System ◽  
Douglas Fir ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase

The inheritance of 27 loci from 18 enzyme systems was investigated using both megagametophyte and embryo tissues of open pollinated seed collected from a natural stand of coastal Douglas-fir [Pseudotsuga menziesii var. menziesii (Mirb.) Franco]. Four enzyme systems - glutamate dehydrogenase (GDH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mannose-6-phosphate isomerase (MPI), and peptidase (PEP) - appeared to be monomorphic and the remaining 14 systems - acid phosphatase (APH), aconitase (ACO), aspartate aminotransferase (AAT), diaphorase (DIA), esterase (EST), glucose-6-phosphate dehydrogenase (G6P), hexoseaminidase (HA), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), malic enzyme (ME), phosphoglucose isomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconic dehydrogenase (6-PGD), and superoxide dismutase (SOD)-showed polymorphism. Each enzyme system was discussed with respect to its multilocus organization, subunit (quaternary) structure, and allelic variation.

scholarly journals Isozyme Markers for Cultivar Identification in Guayule

HortScience ◽  
1990 ◽  
Vol 25 (3) ◽  
pp. 346-348 ◽  
Author(s):  
A. Estilai ◽  
A. Hashemi ◽  
J.G. Waines
Keyword(s):  
Triosephosphate Isomerase ◽  
Cultivar Identification ◽  
Isozyme Variation ◽  
Parthenium Argentatum ◽  
Shikimate Dehydrogenase ◽  
Leaf Extracts ◽  
Banding Patterns ◽  
Isozyme Markers ◽  
Enzyme Systems ◽  
Glutamate Oxalacetate Transaminase

Leaf extracts of 500 plants from 47 guayule (Parthenium argentatum Gray) entries including AZ-101, Gila, Cal-3, Cal-6, and Cal-7 germplasms; 12 accessions from Mexico; and a diverse array of diploid, triploid, and tetraploid selections were analyzed for isozyme variation of 17 enzyme systems. Glutamate oxalacetate transaminase (GOT, EC 2.6.1.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), malate dehydrogenase (MDH, EC 1.1.1.37), phosphoglucoisomerase (PGI, EC 5.3.1.9), shikimate dehydrogenase (SKDH, EC 1.1.1.25), and triosephosphate isomerase (TPI, EC 5.3.1.1) produced sharp and well-resolved bands. With the exception of AZ-101 and Gila, intra- and inter-accession polymorphisms were present for the above enzymes. Plants of AZ-101 and Gila showed identical banding patterns for every enzyme, supporting the view that these two germplasms may be the apomictic progenies of a single selection. Isozyme variations within entries indicated that most of the available guayule germplasms and selections are heterogeneous. Differences between entries suggested that isozymes may provide useful markers for cultivar identification.

scholarly journals Identifying Olive Cultivars by Isozyme Analysis

1995 ◽  
Vol 120 (2) ◽  
pp. 318-324 ◽  
Author(s):  
Isabel Trujillo ◽  
Luis Rallo ◽  
Pere Arús
Keyword(s):  
Gel Electrophoresis ◽  
Malic Enzyme ◽  
Leucine Aminopeptidase ◽  
Morphological Characteristics ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Glucose Phosphate ◽  
Olive Cultivars ◽  
Starch Gel ◽  
Different Origins

Pollen samples of 155 olive (Olea europaea L.) cultivars from different origins were analyzed to study isoenzymatic variability in five enzyme systems: alcohol dehydrogenase (ADH), esterase (EST), glucose phosphate isomerase (GPI), leucine aminopeptidase (LAP), and malic enzyme (ME) using starch gel electrophoresis. Polymorphism was observed in all of the isozyme systems. ME, GPI, EST, and LAP were the most useful systems for identification of cultivars. Different combinations of banding patterns of these systems allowed us to identify 85% of the cultivars. The remainder were separated into groups of two or three cultivars that could be identified using morphological characteristics. No intracultivar polymorphisms were observed.

scholarly journals Linkage Analysis of Ten Isozyme Genes in F1 Segregating Almond Progenies

1994 ◽  
Vol 119 (2) ◽  
pp. 339-344 ◽  
Author(s):  
Pere Arús ◽  
Carmen Olarte ◽  
Miguel Romero ◽  
Francisco Vargas
Keyword(s):  
Leucine Aminopeptidase ◽  
Recombination Fraction ◽  
Joint Estimation ◽  
Linkage Data ◽  
Linkage Groups ◽  
Shikimate Dehydrogenase ◽  
Prunus Amygdalus ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Enzyme Systems

Ten isozyme genes were studied after analyzing the variability of eight enzyme systems—glucose phosphate isomerase (GPI), phosphoglucomutase (PGM), aspartate aminotransferase (AAT), leucine aminopeptidase (LAP), 6-phosphogluconate dehydrogenase (6PGD), isocitrate dehydrogenase (IDH), shikimate dehydrogenase (SDH), and aconitase (ACO)—in the progeny of five crosses among almond [Prunus amygdalus Batsch, syn. P. dulcis (Miller) D. A. Webb] cultivars. Six of these loci were found to be located in two linkage groups, one containing four loci (Pgm-2, Gpi-2, Aat-2, and Lap-1) and two more in the other (Idh-2 and Aat-1). Genetic configurations of pairs of loci specific to segregating F1 progeny of crosses between heterozygous parents were found in our data, for which we derived the estimate of the recombination fraction and its variance. Linkage data for the gene pairs that could be estimated in various crosses were used to obtain a joint estimation of the recombination fraction.

scholarly journals Cultivar Identification and Genetic Diversity within a Hatiora (Cactaceae) Clonal Germplasm Collection Using Isozymes

1999 ◽  
Vol 124 (4) ◽  
pp. 373-376
Author(s):  
Maureen C. O'Leary ◽  
Thomas H. Boyle
Keyword(s):  
Genetic Diversity ◽  
Aspartate Aminotransferase ◽  
Triosephosphate Isomerase ◽  
Leucine Aminopeptidase ◽  
Cultivar Identification ◽  
Germplasm Collection ◽  
Shikimate Dehydrogenase ◽  
Isozyme Markers ◽  
Enzyme Systems ◽  
Entire Collection

Isozyme markers were used to identify cultivars and assess the genetic diversity within a germplasm collection of 49 Hatiora Britt. & Rose clones. The collection included accessions of Easter cactus [H. gaertneri (Regel) Barthlott, H. graeseri Barthlott ex D. Hunt, and H. rosea (Lagerheim) Barthlott] plus H. herminiae (Campos-Porto & Castellanos) Backeberg ex Barthlott and H. salcornioides (Haworth) Britton & Rose. Seven enzyme systems were analyzed: aspartate aminotransferase, glucose-6-phosphate isomerase, leucine aminopeptidase, malate dehydrogenase, phosphoglucomutase, shikimate dehydrogenase, and triosephosphate isomerase. Thirteen loci and 42 alleles were identified. Twenty-one clones (43%) displayed unique isozyme profiles, but the remaining 28 clones shared isozyme profiles with one to three other clones. Percent polymorphic loci, mean number of alleles per locus, and mean heterozygosity were 69, 3.23, and 0.30, respectively, for the entire collection. Isozymes also proved useful for verifying that some progeny were genuine F1 hybrids.

scholarly journals Isoenzyme polymorphism of almond genotypes selected in the region of northern Serbia

2010 ◽  
Vol 37 (No. 2) ◽  
pp. 56-61 ◽  
Author(s):  
S. Čolić ◽  
D. Milatović ◽  
D. Nikolić ◽  
G. Zec
Keyword(s):  
Cluster Analysis ◽  
Alkaline Phosphatase ◽  
Gel Electrophoresis ◽  
Malic Enzyme ◽  
Formate Dehydrogenase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Prunus Dulcis ◽  
Enzyme Systems ◽  
Isoenzyme Polymorphism ◽  
First Time

Isoenzyme polymorphism was studied in 20 almond (Prunus dulcis [Mill.] D.A. Webb) genotypes selected from seedling populations of unknown almond cultivars in the region of northern Serbia (Vojvodina). Fourteen enzyme systems were studied using the method of vertical polyacrylamide gel electrophoresis. Ten systems were polymorphic in twelve loci. This polymorphism allowed unique identification of all studied genotypes. The most useful enzyme for analysis of almond genetic variability was menadione reductase. Polymorphism identified for alkaline phosphatase, formate dehydrogenase, glutamate dehydrogenase, malic enzyme, and menadione reductase was reported for the first time in almond. Cluster analysis was used to construct a dendrogram on which five clusters with different number of genotypes could be identified.

scholarly journals Enzyme Polymorphism in Cymbidium Orchid Cultivars and Inheritance of Leucine Aminopeptidase

HortScience ◽  
1997 ◽  
Vol 32 (7) ◽  
pp. 1267-1271 ◽  
Author(s):  
P. Obara-Okeyo ◽  
Kouei Fujii ◽  
Shunji Kako
Keyword(s):  
Triosephosphate Isomerase ◽  
Leucine Aminopeptidase ◽  
Enzyme Polymorphism ◽  
Starch Gel Electrophoresis ◽  
Shikimate Dehydrogenase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Segregation Ratios ◽  
Starch Gel ◽  
Isozyme Variability

Seventy Cymbidium (Swartz.) cultivars were analyzed for isozyme variability in eight enzyme systems by starch gel electrophoresis. All systems studied [aspartate aminotransferase (AAT), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), phosphoglucomutase (PGM), glucose phosphate isomerase (GPI), triosephosphate isomerase (TPI), and shikimate dehydrogenase (SKDH)] showed polymorphism. When all enzyme systems were evaluated, 68 of the 70 Cymbidium cultivars could be distinguished. Isozymes could not distinguish betwen the cultivars Golden Star `Kumamoto' and Golden Star `Sunrise'. No cultivar showed a single unique pattern, but the TPI system gave one “diagnostic” pattern. Segregation ratios from controlled crosses suggested that LAP-1 is simply inherited and controlled by at least two alleles.

scholarly journals Diversity of Common Bean Landraces Collected in the Western and Eastern Carpatien

2011 ◽  
Vol 39 (No. 3) ◽  
pp. 73-83 ◽  
Author(s):  
O. Horňáková ◽  
M. Závodná ◽  
M. Žáková ◽  
J. Kraic ◽  
F. Debre
Keyword(s):  
Cluster Analysis ◽  
Common Bean ◽  
Morphological Characters ◽  
Molecular Data ◽  
Seed Traits ◽  
Phaseolus Vulgaris L ◽  
Growth Type ◽  
Banding Patterns ◽  
Polymorphism Detection ◽  
Thousand Seed Weight

The study of diversity in common bean was based on morphological and agronomical characteristics, differentiation of collected accessions by morphological and molecular markers, detection of genetic variation, and duplicates detection in bean landraces. The analysed 82 accessions of common bean (Phaseolus vulgaris L.) were collected in the Western andEastern Carpatien as landrace mixtures. Their seeds were segregated and pooled according to their characteristics; they were further multiplicated, and introduced into the collection. An extensive variation in plant and seed traits was discovered in thirty-three morphological and agronomical characteristics. Nevertheless, some of the accessions were identical in these characteristics. Cluster analysis grouped genotypes into two main branches, reflecting the growth type, seed size parameters, and thousand-seed weight. Molecular differentiation studies were performed by multilocus polymorphism detection in microsatellite and minisatellite DNA regions. Cluster analysis based on molecular data also grouped genotypes but no linkage to morphological traits was revealed. Bean accessions with very similar or identical morphological characters were clearly distinguished by DNA banding patterns. The presence of duplicates was excluded.  

Inheritance of isozymes in seed and bud tissues of blue and Engelmann spruce

Genome ◽  
1987 ◽  
Vol 29 (2) ◽  
pp. 239-246 ◽  
Author(s):  
S. G. Ernst ◽  
D. E. Keathley ◽  
J. W. Hanover
Keyword(s):  
Acid Phosphatase ◽  
Key Words ◽  
Malate Dehydrogenase ◽  
Glutamate Oxaloacetate Transaminase ◽  
Blue Spruce ◽  
Mating Design ◽  
Engelmann Spruce ◽  
Enzyme Systems ◽  
Segregation Ratios ◽  
Mode Of Inheritance

Thirteen loci from 11 enzyme systems were identified among full-sib and half-sib progeny of blue and Engelmann spruce. Eleven of the loci were expressed in bud, embryo, and megagametophyte tissue; the remaining two loci were expressed only in embryo and megagametophyte tissue. There were no mobility differences observed between loci expressed in seed and bud tissues. The mode of inheritance for 10 of the loci was confirmed based on progeny genotypic distributions. For the two loci not expressed in bud tissue, acid phosphatase (Acp-2) and diaphorase (Dia-2), inheritance was inferred from pooled segregation ratios of megagametophytes from open-pollinated seed from heterozygous females. The inheritance of glutamate oxaloacetate transaminase (Got-3) was also inferred from segregation ratios and diploid embryo phenotypes of open-pollinated progeny owing to a lack of variability at this locus among the 40 parents in the mating design. Two loci, aldolase (Ald) and malate dehydrogenase (Mdh-2), were monomorphic among the 20 parents of both species. Key words: isozymes, Engelmann spruce, blue spruce, Picea.

Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Schistosoma Mansoni ◽  
Isoelectric Focusing ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

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