scholarly journals 569 PB 012 A MODIFIED FORCING SOLUTION SYSTEM PROMOTES PROPAGATION OF WOODY SPECIES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 513c-513
Author(s):  
Guochen Yang ◽  
Paul E. Read
Keyword(s):  
Root Length ◽  
Woody Species ◽  
Shoot Elongation ◽  
Bud Break ◽  
Root Number ◽  
Negative Effects ◽  
Solution System ◽  
Forcing Solution

A forcing solution containing 200 mg 8-hydroxyquinoline citrate per liter and 2% sucrose has enhanced availability of cutting materials by forcing dormant woody stems in the off-season. Anxins, such as IBA, included in the forcing solution promoted subsequent rooting by increasing root number per cutting and root length for privet. Inclusion of IBA in the forcing solution following the initial use of GA3 in the forcing solution counteracted the undesirable effects of GA3 on rooting and stimulated rooting after taking advantage of the favorable effects of GA3 on bud break and shoot elongation. However, the ability of IBA to counteract the negative effects of GA3 on rooting was dependent on the length of GA3 treatment. The modification of forcing solution system by sequentially including GA3 and then replacing GA3 with IBA expedited propagation of privet. Production of candidate cuttings or explants was stimulated by including GA3 in the forcing solution, and rooting of the cuttings was promoted by subsequent auxin or cytokinin inclusions in the forcing solution to replace GA3 This modified forcing solution system also proved to be a successful and efficient model for propagation of other difficult to propagate woody species.

scholarly journals SEQUENCING OF FORCING SOLUTION PLANT GROWTH REGULATORS ENHANCES MACROPROPAGATION OF LIGUSTRUM VULGARE L. BY SOFTWOOD CUTTINGS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 608a-608
Author(s):  
Guochen Yang ◽  
Paul E. Read
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Woody Plants ◽  
Woody Plant ◽  
Shoot Elongation ◽  
Root Number ◽  
Negative Effects ◽  
Rooting Of Cuttings ◽  
Forcing Solution

A forcing solution containing 200 mg 8-hydroxyquinoline citrate per liter and 2% sucrose has been demonstrated to extend the season for obtaining softwood growth suitable for use as explants in micropropagation (Yang & Read 1989). Forcing dormant woody stems in the off-season in this fashion also enhances the macropropagation of woody plant species by providing softwood outgrowth that can be rooted as softwood cuttings. GA3, IBA, IAA and NAA were incorporated into softwood growth which was later used as cuttings for rooting by adding plant growth regulators at various concentrations to the forcing solution. GA3 incorporated into the forcing solution hastened bud break, increased shoot elongation, but inhibited rooting of softwood cuttings taken from stems forced in this manner. IBA, IAA and NAA in the forcing solution exhibited typical auxin effects on rooting of cuttings by increasing root number per cutting and root elongation. In order to expedite macropropagation of woody plants, GA3 and IBA were added SEQUENTIALLY to the forcing solution. Addition of IBA to fresh forcing solution following initial use of GA3 in the forcing solution counteracted the negative effects of GA3 and stimulated rooting. This protocol is proposed as a method to assist propagation in rooting difficult species by softwood cuttings in the off-season.

scholarly journals PRE-FORCING TREATMENTS HELP BREAK BUD DORMANCY OF FORCED WOODY PLANT SPECIES

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 685d-685
Author(s):  
Guochen Yang ◽  
Paul E. Read
Keyword(s):  
Woody Plant ◽  
Woody Species ◽  
Optimum Concentration ◽  
Bud Dormancy ◽  
Bud Break ◽  
Negative Effects ◽  
Positive Effects ◽  
Forcing Solution ◽  
Almost All

A certain period of cold is needed to break bud dormancy for almost all woody species. A pre-forcing bleach soak has been demonstrated to at least partially replace this requirement (Yang and Read, 1989). Therefore, new softwood growth can be produced in the off-season. Such supple softwood growth is excellent material to be used either as explants for in vitro culture, or as cuttings for macropropagation of woody species. Further studies on pre-forcing bleach soaks were conducted to investigate optimum concentration and duration of soak, and to find the most suitable depth of bleach solution soak, in order to maximize the breaking of bud dormancy. Optimum bud break was obtained by soaking the basal 1/3 of dormant stems in 10% bleach solution for 10 minutes prior to forcing. Soaking dormant woody stems in alcohol solutions prior to placing stems in the forcing solution was also studied. The alcohol soak had negative effects on bud break of spirea, although it showed positive effects for lilac and privet.

Temporal variations in the rooting ability of cuttings of Populusbalsamifera and Salixplanifolia from natural clones–populations of subarctic Quebec

1993 ◽  
Vol 23 (12) ◽  
pp. 2603-2608 ◽  
Author(s):  
Gilles Houle ◽  
Patrice Babeux
Keyword(s):  
Root Length ◽  
Adventitious Roots ◽  
Growing Season ◽  
Temporal Variations ◽  
Bud Break ◽  
Root Number ◽  
Rooting Ability ◽  
Coefficients Of Variation ◽  
Site Restoration ◽  
Great Root

Temporal variations in the rooting ability of cuttings from five clones of Populusbalsamifera L. and five populations of Salixplanifolia Pursh from the Great Whale River valley in subarctic Quebec were determined. Cuttings were sampled monthly from May through October and rooted in a greenhouse. Root number and length of the longest root per cutting were determined 35 days after planting. There were significant variations among the six sampling dates in the rooting potential of cuttings of both species. A higher percentage of cuttings formed adventitious roots in May and June before or shortly after bud break than later, during the growing season; root number and length followed a similar trend. There was an increase in the rooting ability of cuttings of both species towards the fall period. Salixplanifolia produced more roots per cutting than P. balsamifera early in the season (i.e., May and June), but later in the season the differences between the two species were not great; root length showed no such trend. Differences among clones–populations in the rooting potential of cuttings were large for both species. Coefficients of variation for root number were lower in spring than later, during the growing season, for both P. balsamifera and S. planifolia. Seasonal trends in coefficients of variation for root length were not as evident as for root number. These results have significant implications for site restoration in the Subarctic. To optimize the rooting ability of cuttings and minimize the differences among clones–populations in rooting potential, cuttings should be sampled early in the season before bud break or shortly thereafter.

scholarly journals Pre-forcing Treatments Influence Bud Break and Shoot Elongation in Forced Woody Species

1992 ◽  
Vol 10 (2) ◽  
pp. 101-103
Author(s):  
Guochen Yang ◽  
Paul E. Read
Keyword(s):  
In Vitro Culture ◽  
Plant Species ◽  
Woody Plants ◽  
Woody Plant ◽  
Woody Species ◽  
Extended Period ◽  
Shoot Elongation ◽  
Bud Break ◽  
Cold Requirement

Abstract Experiments were conducted to evaluate the feasibility of pre-forcing treatments for the release of bud dormancy of dormant stems of lilac, privet and Vanhoutte spirea. The new softwood growth of these dormant stems was used either as explants for in vitro culture or as cuttings for rooting studies of woody plant species in the off-season. A pre-forcing 15% bleach solution (0.78% NaOCl) soak hastened bud break, enhanced percentage of bud break, and promoted shoot elongation. Pre-forcing wetting agent treatments produced similar results to those of the bleach soak with variation among wetting agents and plant species. Smaller treatment differences were observed in the forcing characteristics when stems were collected later in the winter, probably because the cold requirement of the buds had been completely or partially met. This technique will provide explants for in vitro culture and softwood cuttings for propagation of woody plants over an extended period.

scholarly journals 107 Silver Thiosulfate (STS) Alone and in Combination with Gibberellic Acid (GA3) in the Forcing Solution Influences Budbreak and Shoot Elongation of Dormant Woody Plant Species

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 460A-460
Author(s):  
Bahget T. Hamooh ◽  
Paul E. Read
Keyword(s):  
Gibberellic Acid ◽  
Effective Treatment ◽  
Woody Plants ◽  
Woody Plant ◽  
Shoot Elongation ◽  
Shoot Length ◽  
Solution System ◽  
Silver Thiosulfate ◽  
Cornus Alba ◽  
Forcing Solution

Research was conducted to further modify the forcing solution system in order to expedite the propagation of woody plants, such as Spiraea canescens, Lonicera maakii, and Cornus alba. Time of immersion in solutions containing 5 mM silver thiosulfate (STS) was compared with the basic forcing solution reported by Yang and Read (1989), a solution containing 200 mg 8-hydroxyquinoline citrate per liter and 2% sucrose. Other treatments employed were gibberellic acid (GA3) 50 mg per liter for 24 h and a combination of STS and GA3 for the same amount of time. Increasing the time in STS solution up to 24 h led to higher percent budbreak and shorter time to budbreak for all the three species examined. The combination of STS and GA3 was the most effective treatment overall in reducing time of budbreak and increasing percent of budbreak. All STS treatments studied showed similar responses in shoot elongation. However, treatments with GA3 alone, and in combination with STS showed more than a doubling in shoot length compared to all STS treatments studied and the control. Implications based on SEM observations will be presented.

scholarly journals The Influence of Various Varieties and Various Liquid Organic Fertilizer to Papaya Plant Seed Growth (Carica papaya L.)

AGRIFOR ◽  
2018 ◽  
Vol 17 (1) ◽  
pp. 153
Author(s):  
Primadiyanti Arsela
Keyword(s):  
Root Length ◽  
Carica Papaya ◽  
Block Design ◽  
Organic Fertilizer ◽  
Root Number ◽  
Plant Seed ◽  
Randomized Block Design ◽  
Seed Growth ◽  
Two Factors ◽  
All Treatment

The main goal of the study was to determine the effect of various  Papaya varieties and liquid organic fertilizer on the growth of Papaya seedlings(Carica papaya L.). The research used Randomized Block Design (RAK) with two factors factorial experimental. The first factor is Variety (V) consists of 3 Papaya varieties: v1= bangkok , v2= red lady , v3= sunrise. The second factor is Fertilizer (C) consists of 3 brands: c1= Em4 10 ml, c2= Green Tonic 10ml, c3= Nasa 10ml. This study has 3 treatment levels consisting of 9 combinations and  repeated 3 times. The results of the study are treatment of various varieties (V) had significant effect on stem diameter and root number. The effect of various liquid organic fertilizer (C) had significant effect on the root length parameters. The interaction all treatment had a very significant effect on the root length and the root number parameter. 

scholarly journals The Use of Bean Sprout Extract as Supplement for the Growth of Plaintain Unti Sayang (Musa paradisiaca L.) by Tissue Culture

2014 ◽  
Vol 2 (1) ◽  
pp. 99
Author(s):  
Nirwana Jufri ◽  
Dr. Abdullah ◽  
Devi Susanti
Keyword(s):  
Tissue Culture ◽  
Root Length ◽  
Culture Media ◽  
Leaf Number ◽  
Wide Field ◽  
Root Number ◽  
Bean Sprout ◽  
Musa Paradisiaca ◽  
Tissue Culture Media ◽  
Growth Quality

One of the problems in plaintain development is on the plant breeding that has been conducted convensionally by using seedlings, so that it requires time and wide field, different treatment, and potentially brings pests and deseases. The bean sprout extract is potentially useful in improving the explant growth quality of plaintain by tissue culture. This study aims to know the influence of bean sprout extract concentration as supplement in tissue culture media upon the explant growth of plaintain Unti sayang (Musa paradisiaca L). There are 4 dosages of bean sprout extract as control. The data was analyzed by using ANOVA and was continued by using BNJ test. The result of the experiment shows that the bean sprout extract influences significatly on the plantlets height, leaf number, root length, and root number. The dosage of 100 g/l and 200 g/l bean sprout extracts provide the best result on the experimenting component of plantlet height, leaf number, root length, and root number. Meanwhile, the experimenting component of leaf number and the wet weight of the plantlets provide the best result on MS without any addition of bean sprout extract.

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