scholarly journals Construction of a Genetic Linkage Map and Locations of Common Blight, Rust, and Web Blight Resistance Loci in Phaseolus vulgaris L. Using Random Amplified Polymorphic DNA (RAPD) Markers

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 820E-820 ◽  
Author(s):  
Geunhwa Jung ◽  
Dermot P. Coyne ◽  
Paul W. Skroch ◽  
James Nienhuis ◽  
E. Arnaud-Santana ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Linkage Map ◽  
Phenotypic Variation ◽  
Fungal Pathogens ◽  
Xanthomonas Campestris ◽  
Rust Resistance ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Interval Mapping ◽  
Web Blight

Common blight, web blight, and rust, incited by the bacterial pathogen Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp) and the fungal pathogens Thanatephorus cucumeris (Frank) Donk (Tc) and Uromyces appendiculatus (Pers.:Pers) Unger, respectively, are important diseases of common beans (Phaseolus vulgaris L.). The objectives of were to construct a linkage map, and to locate CBB, rust, and WB resistances and plant architecture traits using RAPDs. Ten linkage groups were identified. Eighty-nine RAPD markers and rust resistance were mapped in 128 RI lines of the cross BAC-6 and HT-7719. Regression analysis and interval mapping using MAPMAKER/QTL were used to identify genomic regions involved in the genetic control of the traits. One, two, two, and three putative QTLs were identified for leaf, seed, and pod reactions to Xcp, and foliar reaction to Tc. These regions accounted for 11%, 9%, 32%, and 30% of the phenotypic variation in the resistances. Two, two, and three regions were identified for plant uprightness, branch density, and pod distribution. These regions accounted for 27%, 13%, and 16% of the phenotypic variation. Unassigned marker G17d influenced some of the phenotypic variation in all three traits. A rust resistance gene controlling pustule size on primary leaves was located in linkage group 1.

scholarly journals 338 CONSTRUCTION OF A GENETIC LINKAGE MAP AND LOCATIONS OF COMMON BLIGHT RESISTANCE LOCI AND RUST ADULT RESISTANCE IN PHASEOLUS VULGARIS L USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 479a-479
Author(s):  
Geunhwa Jung ◽  
Dermot P. Coyne ◽  
E. Arnaud-Santana ◽  
James Bokosi ◽  
Shawn M. Kaeppler ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Linkage Group ◽  
Linkage Map ◽  
Xanthomonas Campestris ◽  
Rapd Markers ◽  
Chromosome Region ◽  
Random Amplified Polymorphic Dna ◽  
Interval Mapping ◽  
Adult Plant ◽  
Leaf Pubescence

Common bacterial blight(CBB) and rust diseases, incited by the bacterial pathogen Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp) and Uromyces appendiculatus, respectively, are important diseases of common beans (Phaseolus vulgaris L.). The objectives were to construct a molecular linkage map, to locate CBB resistances, rust resistances and leaf pubescence using RAPDs. Sixteen linkage groups with 22 unassigned markers were identified. 178 RAPD markers and 8 morphological markers were mapped in a Population of 70 RI lines. Regression analysis and interval mapping using MAPMAKER/QTL were used to identify genomic regions involved in the genetic control of the traits. One, two, and three putative QTLs were identified for seed, pod and leaf reactions. These regions accounted for 18%, 25%, and 35% of the phenotypic variation in CBB resistance. A chromosome region on linkage group 1 carried factors influencing all three traits. Rust resistance genes controlling the reactions on the primary and on the 4th trifoliolate leaves (adult plant resistance) were located in linkage group 16. The genes for abaxial leaf pubescence was located on linkage group 9.

scholarly journals Construction of a Genetic Linkage Map and Locations of Halo Blight and Brown Spot Resistance Loci in Common Bean (Phaseolus vulgaris L.) using RAPD Markers

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 451D-451
Author(s):  
H.M. Ariyarathne ◽  
Dermot P. Coyne ◽  
Geunhwa Jung
Keyword(s):  
Linkage Map ◽  
Pseudomonas Syringae ◽  
Phenotypic Variation ◽  
Rapd Markers ◽  
Major Gene ◽  
Interval Mapping ◽  
Brown Spot ◽  
Halo Blight ◽  
Genomic Regions ◽  
Ri Lines

Halo blight (HB), brown spot (BS), and rust incited by the bacterial pathogens Pseudomonas syringae pv. phaseolicola (Psp), Pseudomonas syringae pv. syringae (Pss) and the fungal pathogen Uromyces appendiculatus, respectively, are important diseases of common beans. The objectives were to construct a RAPD linkage map, and to locate HB and BS resistance genes and genes for some other traits. One-hundred-seventy RAPD markers were mapped in 78 RI lines of the cross BelNeb 1 and A 55. Eleven main and nine minor linkage groups were identified. MAPMAKER/QTL, interval mapping, was used to identify genomic regions involved in the genetic control of the traits. One region was found to control HB leaf reactions to strain HB16 while three regions controlled reactions to strain HB 83. These regions accounted for 22% and 18%, 17%, and 17% of phenotypic variation of resistance, respectively. Four putative QTLs were identified for resistance to BS, and accounted for 37%, 26%, 23%, and 19% of the phenotypic variation. Rust resistance was determined by a single major gene to both rust strains US85NP 5-1 and D82vc74fh. However, linked markers were not identified. The V gene controlling flower and stem color was tightly linked with the Operon marker O10.620.

scholarly journals Molecular Markers Associated with Plant Architecture and Resistance to Common Blight, Web Blight, and Rust in Common Beans

1996 ◽  
Vol 121 (5) ◽  
pp. 794-803 ◽  
Author(s):  
Geunhwa Jung ◽  
Dermot P. Coyne ◽  
Paul W. Skroch ◽  
James Nienhuis ◽  
E. Arnaud-Santana ◽  
...  
Keyword(s):  
Common Bean ◽  
Linkage Map ◽  
Recombinant Inbred ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Random Amplified Polymorphic Dna ◽  
Recombinant Inbred Lines ◽  
Rapd Marker ◽  
Common Bacterial Blight ◽  
Web Blight

Random amplified polymorphic DNA (RAPD) markers were used to construct a partial linkage map in a recombinant inbred population derived from the common bean (Phaseolus vulgaris L.) cross BAC 6 × HT 7719 for studying the genetics of disease resistance in common bean. The linkage map spanned 545 cM and included 75 of 84 markers used in this study. The population of 128 recombinant inbred lines was evaluated for resistance to common bacterial blight, foliar resistance to web blight [WB; Thanatephorus cucumeris (Frank) Donk], and resistance to rust [Uromyces appendiculatus var. appendiculatus (Pers.:Pers) Unger]. Common bacterial blight [CBB; Xanthomonas campestris pv. phaseoli (Smith) Dye] resistance was evaluated for CBB strain Epif-IV in later-developed trifoliolate leaves and for CBB strain EK-11 in seeds, first trifoliolate leaves, later-developed trifoliolate leaves, and pods. In addition, lines were rated for plant uprightness and branch density. Two to six markers accounted for 14% to 34% of the phenotypic variation for each trait. Significant marker locustrait associations were found for 14 mapped loci and 7 of the 9 unmapped markers. The distribution of detected QTL appeared to be nonrandom with most significant markers associated with more than one trait or closely linked to markers significantly associated with variation for a different trait. One marker, BC4091250, was significantly associated with WB resistance, resistance for CBB strain Epif-IV in later-developed trifoliolate leaves, and resistance for CBB strain EK-11 in first trifoliolate leaves, later-developed trifoliolate leaves, and pods. A rust resistance gene was mapped in an interval 14.6 cM from RAPD marker H191050 and 12.5 cM from marker AJ16250.

scholarly journals Quantitative Trait Loci (QTL) Analysis of Canning Quality Traits in Kidney Bean (Phaseolus vulgaris L.)

2002 ◽  
Vol 127 (4) ◽  
pp. 608-615 ◽  
Author(s):  
Maria-Carmela T. Posa-Macalincag ◽  
George L. Hosfield ◽  
Kenneth F. Grafton ◽  
Mark A. Uebersax ◽  
James D. Kelly
Keyword(s):  
Phaseolus Vulgaris ◽  
Linkage Group ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Recombinant Inbred Lines ◽  
Kidney Bean ◽  
Major Genes ◽  
The Core ◽  
Two Populations ◽  
Canning Quality

Canning quality of dry bean (Phaseolus vulgaris L.), of which the degree of splitting (SPLT) and overall appearance (APP) of canned beans are major components, is a complex trait that exhibits quantitative inheritance. The objectives of this study were to identify major genes that affect APP and SPLT in kidney bean, and map the location of these loci to the integrated core map of common bean. The analysis was performed using random amplified polymorphic DNA (RAPD) markers and two populations of kidney bean, consisting of 75 and 73 recombinant inbred lines (RILs), respectively. The two populations—`Montcalm' × `California Dark Red Kidney 82' and `Montcalm' × `California Early Light Red Kidney'—were planted in six year-location combinations in Michigan, Minnesota and North Dakota from 1996 to 1999. Correlations between APP and SPLT were high (0.91 to 0.97). Heritability estimates for APP and SPLT ranged from 0.83 to 0.85 in the two populations. Major genes for these traits were identified on two linkage groups. The first QTL, associated with seven RAPD markers, was putatively mapped to the B8 linkage group of the core bean linkage map. Desirable canning quality appeared to be derived from Montcalm at this locus. The second QTL, associated with four markers, appeared to be derived from the California parents. The second linkage group was not assigned to a linkage group in the core map. Population and environment-specificity were observed for the markers identified.

Linkage of random amplified polymorphic DNA, isozyme and morphological markers in grasspea (Lathyrus sativus)

1999 ◽  
Vol 133 (4) ◽  
pp. 389-395 ◽  
Author(s):  
M. A. CHOWDHURY ◽  
A. E. SLINKARD
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Random Amplified Polymorphic Dna ◽  
Lathyrus Sativus ◽  
Morphological Markers ◽  
Linkage Studies ◽  
Linkage Groups

We constructed a genetic linkage map of grasspea (Lathyrus sativus L.; 2n = 14) from 100 F2 individuals derived from a cross between PI 426891.1.3 and PI 283564c.3.2. A total of 71 RAPD, three isozyme and one morphological markers segregated in the F2 progeny. A small fraction of markers (12%) deviated significantly from the expected Mendelian ratio (1[ratio ]2[ratio ]1 or 3[ratio ]1). Out of 75 markers, 69 (one morphological, three isozyme and 65 RAPD markers) were assigned to 14 linkage groups comprising 898 cM. The average distance between two adjacent markers was 17·2 cM. The present linkage map will serve as a reference point for further linkage studies in grasspea.

Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1050-1056 ◽  
Author(s):  
V Chagué ◽  
T Fahima ◽  
A Dahan ◽  
G L Sun ◽  
A B Korol ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Rapd Markers ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Stripe Rust Resistance Gene

Microsatellite and random amplified polymorphic DNA (RAPD) primers were used to identify molecular markers linked to the Yr15 gene which confer resistance to stripe rust (Puccina striiformis Westend) in wheat. By using near isogenic lines (NILs) for the Yr15 gene and a F2 mapping population derived from crosses of these lines and phenotyped for resistance, we identified one microsatellite marker (GWM33) and one RAPD marker (OPA19800) linked to Yr15. Then, bulked segregant analysis was used in addition to the NILs to identify RAPD markers linked to the target gene. Using this approach, two RAPD markers linked to Yr15 were identified, one in coupling (UBC199700) and one in repulsion phase (UBC2121200). After Mapmaker linkage analysis on the F2 population, the two closest markers were shown to be linked to Yr15 within a distance of about 12 cM. The recombination rates were recalculated using the maximum likelihood technique to take into account putative escaped individuals from the stripe rust resistance test and obtain unbiased distance estimates. As a result of this study, the stripe rust resistance gene Yr15 is surrounded by two flanking PCR markers, UBC199700 and GWM33, at about 5 cM from each side.Key words: wheat, Triticum dicoccoides, Yr15 stripe rust resistance gene, genetic mapping, microsatellite markers, RAPD markers.

The first linkage map of the plant-pathogenic basidiomyceteTyphula ishikariensis

Genome ◽  
2008 ◽  
Vol 51 (2) ◽  
pp. 128-136 ◽  
Author(s):  
S. W. Chang ◽  
G. Jung
Keyword(s):  
Linkage Map ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Fungal Growth ◽  
Aerial Mycelium ◽  
Creeping Bentgrass ◽  
Inter Simple Sequence Repeat ◽  
Perennial Grasses ◽  
Hyphal Fusion ◽  
Simple Sequence

Speckled snow mold, caused by the basidiomycete Typhula ishikariensis Imai, is one of the most prominent winter diseases on perennial grasses and cereal crops in the northern hemisphere. The first linkage map of T. ishikariensis was constructed using a population of 93 sibling monokaryons derived from a single dikaryotic hybrid isolate that was created by a hyphal fusion of two monokaryotic parental isolates. The parental isolates were produced from a pathogenic dikaryotic isolate collected from a golf course in Wisconsin. The two parents exhibit significant differences in the production of aerial mycelium and sclerotia, and in their aggressiveness on creeping bentgrass ( Agrostis stolonifera L.). A total of 251 loci were mapped, comprising 89 inter-simple sequence repeat (ISSR) and 160 random amplified polymorphic DNA (RAPD) markers along with 2 phenotype-based mating-type (MAT) loci. The MAT loci were mapped on linkage groups (LGs) 1 and 7. The markers were evenly distributed over 7 LGs, covering 436 cM with an average marker interval of 2.2 cM. Seven chromosomes were cytologically observed using germ tube bursting methods with acetocarmine staining. This reference linkage map of T. ishikariensis should provide a framework for the mapping of quantitatively controlled traits such as fungal growth, survival, and virulence/avirulence under low temperatures. The map should also be utilized for studying the genome organization of the cold-loving plant-pathogenic Typhula spp. and for comparative genome analysis among fungal taxa.

scholarly journals Linkage map of the honey bee, Apis mellifera, based on RAPD markers.

Genetics ◽  
1995 ◽  
Vol 139 (3) ◽  
pp. 1371-1382 ◽  
Author(s):  
G J Hunt ◽  
R E Page
Keyword(s):  
Linkage Map ◽  
Honey Bee ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Black Body ◽  
Linkage Groups ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Haploid Male ◽  
Relationship Of

Abstract A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female bee. The X locus for sex determination and genes for black body color and malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be approximately 3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.

scholarly journals Identification of RAPD Markers Linked to Five Marker Genes (blu, dgs, y, arg, and a Flat Pod Mutant) in Common Bean

2002 ◽  
Vol 127 (4) ◽  
pp. 685-688 ◽  
Author(s):  
Gino E. Beltrán ◽  
Geunwha Jung ◽  
James Nienhuis ◽  
Mark J. Bassett
Keyword(s):  
Molecular Markers ◽  
Common Bean ◽  
Linkage Map ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Spontaneous Mutation ◽  
Marker Genes ◽  
Dark Green ◽  
Mapping Populations

The development of a complete linkage map, including both classical (visible) and molecular markers, is important to understand the genetic relationships among different traits in common bean (Phaseolus vulgaris L.). The objective of this study was to integrate classical marker genes into previously constructed molecular linkage maps in common bean. Bulked segregant analysis was used to identify 10 random amplified polymorphic DNA (RAPD) markers linked to genes for five classical marker traits: dark green savoy leaf (dgs), blue flower (blu), silvery [Latin: argentum] green pod (arg), yellow wax pod (y) and flat pod (a spontaneous mutation from round to flat pod in `Hialeah' snap bean). The genes for dark green savoy leaf (dgs) and blue flower (blu) were located in a previously constructed molecular linkage map. These results indicate that classical marker genes and molecular markers can be integrated to form a more complete and informative genetic linkage map. Most of the RAPD markers were not polymorphic in the two mapping populations used, and molecular markers from those mapping populations were not polymorphic in the F2 populations used to develop the RAPD markers. Alternative genetic hypotheses for the pod shape mutation in `Hialeah' are discussed, and the experimental difficulties of pod shape classification are described.

Construction of a Linkage Map and QTL analysis for Black Rot Resistance in Brassica oleracea L.

1970 ◽  
Vol 1 (1) ◽  
pp. 1-6 ◽  
Author(s):  
MAU Doullah ◽  
GM Mohsin ◽  
K Ishikawa ◽  
H Hori ◽  
K Okazaki
Keyword(s):  
Linkage Map ◽  
Brassica Oleracea ◽  
Phenotypic Variation ◽  
Xanthomonas Campestris ◽  
Resistance Breeding ◽  
Black Rot ◽  
Significant Qtls ◽  
Disease Rating ◽  
Genomic Regions ◽  
Xanthomonas Campestris Pv Campestris

For quantitative trait loci (QTL) controlling resistance to Xanthomonas campestris pv. Campestris, we constructed linkage map using cleaved amplified plymorphic sequences (CAPS) and sequence-related amplified polymorphism (SRAP) analysis with disease rating of F3 families obtained from a susceptible broccoli and resistant cabbage [Green commet P09 × Reiho P01]. We established inoculation technique. In this technique, leaves from approximately 50-day old F3 plants were inoculated by cutting 1.0 cm at mid vain near the margins. A total of 38 CAPS and 60 SRAP primer pairs were screened to assess parental polymorphism against black rot resistance. Ninety two markers were distributed in 10 linkage groups (LGs) covering 320.5 cM (centimorgan), with average 3.56 cM interval between markers. Two genomic regions on LG 2 and LG 9 were significantly associated with resistance to the disease. The analysis revealed QTLs in the map interval between CAM1 – GSA1 on LG 2 accounting for up to 10% of the phenotypic variation and one QTL in the map interval between F12-R12e – BORED on LG 9 explaining 16% phenotypic variation with LOD score of 3.09. Two additional non-significant QTLs on LG 3 in the interval between CHI – ASB1 (LOD = 2.04) and on LG 7 in the interval between IPI – FLC3 (LOD = 2.25) were also detected for resistance to the disease. The QTLs, which were mapped to LG 2 and LG 9 for the disease, could be useful for marker-assisted selection in resistance breeding. Key words: Linkage map; QTL; Black rot; Resistance; Brassica oleracea  DOI: http://dx.doi.org/10.3329/ijns.v1i1.8591 International Journal of Natural Sciences (2011), 1(1):1-6

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