Transformation of `Bosc' Pear (Pyrus communis L.) with the rolC Gene from Agrobacterium rhizogenes and Characterization of Transgenic Plants

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 461c-461
Author(s):  
Ralph Scorza ◽  
Richard L. Bell ◽  
Chinnathambi Srinivasan ◽  
Kevin Webb
Keyword(s):  
Plant Growth ◽  
Genetic Transformation ◽  
Genetic Improvement ◽  
Size Control ◽  
The United States ◽  
Tree Size ◽  
Native Promoter ◽  
Pyrus Communis L ◽  
Size Controlling

Pear production in the United States relies on a few major cultivars, including `Bosc'. While there is a need for new cultivars, genetic improvement of the existing major cultivars through genetic transformation could have a major impact on the industry. We have developed a system for regeneration and transformation of pear. While the major objective of the transformation project is to improve resistance to fire blight (Erwinia amylovora) in major pear cultivars, tree size control is also one of the objectives in the genetic improvement of pear that may be approached through transformation. Traditionally, manipulation of tree size and vigor in established cultivars has been achieved through the use of size-controlling rootstocks. There are no completely satisfactory size-controlling rootstocks for pear. Genetic transformation provides an approach to developing new size-controlling rootstocks and also to directly affect the growth of the transgenic scion cultivar using genes that affect plant growth such as the rolC gene isolated from the bacterium A. rhizogenes, the causal agent of “hairy root” disease. This gene has been shown to alter growth and development in a number of plant species. To investigate the potential utility of the rolC gene in altering the growth of pear trees, `Bosc' pear was transformed with A. tumefaciens EHA101 carrying a pGA482-based plasmid containing the NPTII and GUS genes, and the rolC gene under the control of its native promoter. Four clones were isolated that were kanamycin-resistant and GUS-positive. PCR assays and DNA blots indicated the presence of the rolC gene in these clones. Each transgenic clone has been multiplied in vitro and planted in the greenhouse where transgene expression and plant growth are being evaluated.

scholarly journals In Planta Genetic Transformation of Mungbean (Vigna radiata (L.) Wilczek) with Marker Gene

2019 ◽  
Vol 29 (2) ◽  
pp. 245-255
Author(s):  
Mohammad Mahmood Hasan ◽  
Sujay Kumar Bhajan ◽  
M. Imdadul Hoque ◽  
R.H. Sarker ◽  
Mohammad Nurul Islam
Keyword(s):  
Genetic Transformation ◽  
Genetic Improvement ◽  
In Vitro Regeneration ◽  
Marker Gene ◽  
Gus Expression ◽  
In Planta ◽  
Leaf Tissues ◽  
Gus Gene Expression ◽  
Screenable Marker

In genetic improvement of mungbean much success has not been achieved due to its recalcitrant nature towards in vitro regeneration. An attempt was made to develop an Agrobacterium-mediated in planta genetic transformation protocol for a locally grown mungbean variety BARI Mung-3 using a screenable marker gene. Two minutes of vacuum infiltration followed by 60 minutes of incubation period in Agrobacterium suspension of Winans’ AB medium containing wounded tobacco leaf extract was found most suitable towards genetic transformation in pricked de-coated half seed explants. An optical density (OD600) of 0.7 was found most effective for transient gus gene expression. Chimeric GUS expression was observed in the root and leaf tissues from the successfully transformed plantlets obtained through in planta transformation. This methodology of genetic transformation was found more suitable, easier and less time consuming than tissue culture based genetic transformation, which may be used for the genetic improvement of mungbean.

Advances in in vitro studies for genetic enhancement of blackgram: A review

2019 ◽  
Author(s):  
Paras Pandey ◽  
Meenal Rathore ◽  
N. P. Singh
Keyword(s):  
Genetic Transformation ◽  
Genetic Improvement ◽  
In Vitro Regeneration ◽  
Vigna Mungo ◽  
In Vitro Studies ◽  
Genetic Enhancement ◽  
Black Gram ◽  
Treasure Trove ◽  
Genetic Base

Black gram (Vigna mungo) is a treasure trove legume in terms of nutritional efficiency and medicinal values and an important pulse that is cultivated throughout India in all three seasons. However, its production and productivity is constrained due to abiotic and biotic stresses.Though technology has aided in genetic improvement of the crop, the narrow genetic base remains a potential constraint. In vitro regeneration and genetic transformation have aided to its genetic improvement, yet a lot remains to be explored and harnessed for efficient and effective genetic improvement of the pulse. The review highlights the progress and achievements in context of in vitro regeneration and genetic transformation in blackgram.

The male gamete as a tool in the genetic improvement of cereals

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 1005-1013 ◽  
Author(s):  
E. Picard
Keyword(s):  
Genetic Transformation ◽  
Genetic Improvement ◽  
In Vitro Selection ◽  
Doubled Haploids ◽  
Male Gamete ◽  
Pollen Culture ◽  
Male Gametes ◽  
Recent Developments ◽  
Breeding Programmes

This review surveys recent developments in the use of male gametes for the genetic improvement of cereals. It focuses mainly on the production of doubled haploids and their use in breeding programmes but also discusses gametoclonal variaton, in vitro selection through anther or pollen culture, and genetic transformation using the pollen tube as a vehicle for foreign DNA.Key words: male gamete, haploids, doubled haploids, cereals, gametoclonal variation, in vitro selection, pollen-mediated genetic transformation.

scholarly journals Creating an Ally from an Adversary: Genetic Manipulation of Citrus Tristeza

2004 ◽  
Author(s):  
William O. Dawson ◽  
Moshe Bar-Joseph
Keyword(s):  
Genetic Improvement ◽  
Target Genes ◽  
Genetic Manipulation ◽  
The United States ◽  
Expression Vectors ◽  
Control Element ◽  
Class Iii ◽  
Cis Acting ◽  
Citrus Tristeza

Citrus is one of the major agricultural crops common to Israel and the United States, important in terms of nutrition, foreign exchange, and employment. The economy of both citrus industries have been chronically plagued by diseases caused by Citrus tristeza virus (CTV). The short term solution until virus-resistant plants can be used is the use of mild strain cross-protection. We are custom designing "ideal" protecting viruses to immunize trees against severe isolates of CTV by purposely inoculating existing endangered trees and new plantings to be propagated as infected (protected) citrus budwood. We crossed the substantial technological hurdles necessary to accomplish this task which included developing an infectious cDNA clone which allows in vitro manipulation of the virus and methods to then infect citrus plants. We created a series of hybrids between decline-inducing and mild CTV strains, tested them in protoplasts, and are amplifying them to inoculate citrus trees for evaluation and mapping of disease determinants. We also extended this developed technology to begin engineering transient expression vectors based on CTV as tools for genetic improvement of tree crops, in this case citrus. Because of the long periods between genetic transformation and the ultimate assay of mature tree characteristics, there is a great need for an effective system that allows the expression or suppression of target genes in fruiting plants. Virus-based vectors will greatly expedite progress in citrus genetic improvement. We characterized several components of the virus that provides necessary information for designing virus-based vectors. We characterized the requirements of the 3 ’-nontranslated replication promoter and two 3 ’-ORF subgenomic (sg) mRNA controller elements. We discovered a novel type of 5’-terminal sgRNAs and characterized the cis-acting control element that also functions as a strong promoter of a 3 ’-sgRNA. We showed that the p23 gene controls negative-stranded RNA synthesis and expression of 3 ’ genes. We identified which genes are required for infection of plants, which are host range determinants, and which are not needed for plant infection. We continued the characterization of native dRNA populations and showed the presence of five different classes including class III dRNAs that consists of infectious and self-replicating molecules and class V dRNAs that contain all of the 3 ’ ORFs, along with class IV dRNAs that retain non-contiguous internal sequences. We have constructed and tested in protoplasts a series of expression vectors that will be described in this proposal.

scholarly journals Plant Regeneration and Genetic Transformation in Buckwheat (Fagopyrum spp.), A Multipurpose Gluten Free Crop of High Nutraceutical Importance: A Critical Review

2018 ◽  
Vol 7 (1) ◽  
pp. 1954 ◽  
Author(s):  
Mithilesh Kumar
Keyword(s):  
Plant Regeneration ◽  
Genetic Transformation ◽  
High Frequency ◽  
Genetic Improvement ◽  
In Vitro Regeneration ◽  
Gluten Free ◽  
Success Rates ◽  
Plant Propagation ◽  
Cultivated Species

Buckwheat (Fagopyrum sp.) from family Polygonaceae, with its two main cultivated species (F. esculentumMoench. and F. tataricum) is mostly grown in hilly regions of Eurasia. It is a multipurpose gluten free crop having great nutraceutical value. For plant propagation several reports have been published and significant differences were noticed in culture protocols, genotypes and their success rates. A few studies report genetic transformation in buckwheat with varying degree of success. The present study reviews the in-vitro regeneration and genetic transformation reports in Fagopyrum spp. available in publically available literature and concludes on what needs to be done for the sustainable genetic improvement of buckwheat. A genotype independent high frequency in-vitro regeneration protocol compatible with high end stable genetic transformation is the need of the hour for sustained genetic improvement of this crop.

Correlative microscopy in distrubuted (client/server) computing environments: tools for catalyzing the rapid dissemination of information about the cell biology of the human prostate

1995 ◽  
Vol 53 ◽  
pp. 682-683
Author(s):  
A. Hakam ◽  
J.T. Gau ◽  
M.L. Grove ◽  
B.A. Evans ◽  
M. Shuman ◽  
...  
Keyword(s):  
United States ◽  
Cell Biology ◽  
Tissue Bank ◽  
The United States ◽  
Prostate Adenocarcinoma ◽  
Correlative Microscopy ◽  
Client Server ◽  
Critical Elements ◽  
Transplant Organ

Prostate adenocarcinoma is the most common malignant tumor of men in the United States and is the third leading cause of death in men. Despite attempts at early detection, there will be 244,000 new cases and 44,000 deaths from the disease in the United States in 1995. Therapeutic progress against this disease is hindered by an incomplete understanding of prostate epithelial cell biology, the availability of human tissues for in vitro experimentation, slow dissemination of information between prostate cancer research teams and the increasing pressure to “ stretch” research dollars at the same time staff reductions are occurring.To meet these challenges, we have used the correlative microscopy (CM) and client/server (C/S) computing to increase productivity while decreasing costs. Critical elements of our program are as follows:1) Establishing the Western Pennsylvania Genitourinary (GU) Tissue Bank which includes >100 prostates from patients with prostate adenocarcinoma as well as >20 normal prostates from transplant organ donors.

Repurposing N,N-Dimethylacetamide (DMA), a Pharmaceutical Excipient, as a Prototype Novel Anti-inflammatory Agent for the Prevention and/or Treatment of Preterm Birth

2018 ◽  
Vol 24 (9) ◽  
pp. 989-992 ◽  
Author(s):  
Samir Gorasiya ◽  
Juliet Mushi ◽  
Ryan Pekson ◽  
Sabesan Yoganathan ◽  
Sandra E. Reznik
Keyword(s):  
Preterm Birth ◽  
Ex Vivo ◽  
The United States ◽  
Occurrence Rate ◽  
Pharmaceutical Excipient ◽  
Ongoing Work ◽  
Exciting Line ◽  
Pregnant Mice

Background: Preterm birth (PTB), or birth that occurs before 37 weeks of gestation, accounts for the majority of perinatal morbidity and mortality. As of 2016, PTB has an occurrence rate of 9.6% in the United States and accounts for up to 18 percent of births worldwide. Inflammation has been identified as the most common cause of PTB, but effective pharmacotherapy has yet to be developed to prevent inflammation driven PTB. Our group has discovered that N,N-dimethylacetamide (DMA), a readily available solvent commonly used as a pharmaceutical excipient, rescues lipopolysaccharide (LPS)-induced timed pregnant mice from PTB. Methods: We have used in vivo, ex vivo and in vitro approaches to investigate this compound further. Results: Interestingly, we found that DMA suppresses cytokine secretion by inhibiting nuclear factor-kappa B (NF-κB). In ongoing work in this exciting line of investigation, we are currently investigating structural analogs of DMA, some of them novel, to optimize this approach focused on the inflammation associated with PTB. Conclusion: Successful development of pharmacotherapy for the prevention of PTB rests upon the pursuit of multiple strategies to solve this important clinical challenge.

Review on the Clinical Pharmacology of Hydroxychloroquine Sulfate for the Treatment of COVID-19

2020 ◽  
Vol 21 (6) ◽  
pp. 427-435 ◽  
Author(s):  
Cheng Cui ◽  
Siqi Tu ◽  
Valerie Sia Jie En ◽  
Xiaobei Li ◽  
Xueting Yao ◽  
...  
Keyword(s):  
Drug Interactions ◽  
Clinical Efficacy ◽  
Relevant Literature ◽  
The United States ◽  
Efficacy And Safety ◽  
Open Label ◽  
Infected People ◽  
Treatment Regimens ◽  
Therapeutic Drugs

Background: As the number of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) infected people is greatly increasing worldwide, the international medical situation becomes very serious. Potential therapeutic drugs, vaccine and stem cell replacement methods are emerging, so it is urgent to find specific therapeutic drugs and the best treatment regimens. After the publications on hydroxychloroquine (HCQ) with anti- SARS-COV-2 activity in vitro, a small, non-randomized, open-label clinical trial showed that HCQ treatment was significantly associated with reduced viral load in patients with coronavirus disease-19 (COVID-19). Meanwhile, a large prophylaxis study of HCQ sulfate for COVID-19 has been initiated in the United States. HCQ offered a promising efficacy in the treatment of COVID-19, but the optimal administration is still being explored. Methods: We used the keyword "hydroxychloroquine" to conduct a literature search in PubMed to collect relevant literature on the mechanism of action of HCQ, its clinical efficacy and safety, pharmacokinetic characteristics, precautions for clinical use and drug interactions to extract and organize information. Results: This paper reviews the mechanism, clinical efficacy and safety, pharmacokinetic characteristics, exposureresponse relationship and precautions and drug interactions of HCQ, and summarizes dosage recommendations for HCQ sulfate. Conclusion: It has been proved that HCQ, which has an established safety profile, is effective against SARS-CoV-2 with sufficient pre-clinical rationale and evidence. Data from high-quality clinical trials are urgently needed worldwide.

scholarly journals Phytase-Producing Rahnella aquatilis JZ-GX1 Promotes Seed Germination and Growth in Corn (Zea mays L.)

2021 ◽  
Vol 9 (8) ◽  
pp. 1647
Author(s):  
Gui-E Li ◽  
Wei-Liang Kong ◽  
Xiao-Qin Wu ◽  
Shi-Bo Ma
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Biomass Accumulation ◽  
Root Surface ◽  
Phosphorus Concentration ◽  
Maize Seedlings ◽  
Total Phosphorus Concentration ◽  
Rahnella Aquatilis ◽  
Maize Seeds

Phytase plays an important role in crop seed germination and plant growth. In order to fully understand the plant growth-promoting mechanism by Rahnella aquatilis JZ-GX1，the effect of this strain on germination of maize seeds was determined in vitro, and the colonization of maize root by R. aquatilis JZ-GX1 was observed by scanning electron microscope. Different inoculum concentrations and Phytate-related soil properties were applied to investigate the effect of R. aquatilis JZ-GX1 on the growth of maize seedlings. The results showed that R. aquatilis JZ-GX1 could effectively secrete indole acetic acid and had significantly promoted seed germination and root length of maize. A large number of R. aquatilis JZ-GX1 cells colonized on the root surface, root hair and the root interior of maize. When the inoculation concentration was 107 cfu/mL and the insoluble organophosphorus compound phytate existed in the soil, the net photosynthetic rate, chlorophyll content, phytase activity secreted by roots, total phosphorus concentration and biomass accumulation of maize seedlings were the highest. In contrast, no significant effect of inoculation was found when the total P content was low or when inorganic P was sufficient in the soil. R. aquatilis JZ-GX1 promotes the growth of maize directly by secreting IAA and indirectly by secreting phytase. This work provides beneficial information for the development and application of R. aquatilis JZ-GX1 as a microbial fertilizer in the future.

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