Vegetative Propagation of Woody Ornamentals Through Forcing of Epicormic Shoots

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 503c-503
Author(s):  
Paul H. Henry ◽  
John E. Preece
Keyword(s):  
Plant Material ◽  
Woody Plant ◽  
Clonal Plant ◽  
Light Regime ◽  
Moisture Regime ◽  
Stem Cuttings ◽  
Shrub Species ◽  
Woody Ornamentals ◽  
Ornamental Tree ◽  
Environmental Light

A propagation method is being developed that allows softwood shoots to be generated from stem sections of dormant woody plant species. These shoots, forced in the greenhouse during the winter, are subsequently collected and processed as softwood stem cuttings to produce clonal plant material. Many species in the nursery industry can only be propagated using softwood cuttings, and this technique allows propagation of these species to be initiated several months earlier than what is typically possible. Current studies involve expanded screening of ornamental tree and shrub species to determine if commercial production using this technique is feasible. Results demonstrate that many species may be propagated using this method, but that some species are more prolific than others with respect to number of softwood shoots produced. Additional studies are currently in progress to determine the environmental (light regime, moisture regime) and cultural (type of media) conditions that are optimal when producing clonal plant material via this technique.

scholarly journals Effects of s-ABA on the Physiology and Marketability of Various Container-Grown Taxa During Short-Term Desiccation

2010 ◽  
Vol 28 (4) ◽  
pp. 202-208
Author(s):  
Cary J. Hebert ◽  
Anthony V. LeBude ◽  
Craig Campbell
Keyword(s):  
Plant Material ◽  
Woody Plant ◽  
Spray Application ◽  
Short Term ◽  
Woody Ornamentals ◽  
Second Stage ◽  
Ligustrum Japonicum ◽  
Retail Setting ◽  
Wilting Point ◽  
Spray Applications

Abstract Reduced post harvest care of woody plant material in mass retail settings can decrease the number of days plants remain marketable. If plants are sold on consignment for growers, reduced sales can lead to poor profitability. This study investigated the effect of spray applications of s-abscisic acid (s-ABA) (ConTego™ Pro SL, Valent Biosciences Corp.) to increase the number of days of marketability for various woody taxa in a simulated retail setting. In the first stage of the study, various well-watered container-grown taxa were treated with a spray application of either 0, 1000 or 2000 mg·liter−1 of s-ABA and water was withheld. Daily, desiccation symptoms were recorded to determine if plants had reached the critical wilting point (CWP) and thus became unmarketable. Marketability was increased approximately 1–7 days for plants treated with 2000 mg·liter−1 of s-ABA compared to nontreated plants. In the second stage, marketability and physiology of Ligustrum japonicum ‘Recurvifolium’ (wavy leaf privet) were monitored after plants were treated with spray applications of 0, 500, 1000, 1500 or 2000 mg·liter−1 of s-ABA and water withheld. Stomatal conductance (gs) declined for all plants depending on the concentration applied. Plants treated with s-ABA had lower gs rates and remained marketable longer than nontreated plants. All plants fully recovered to pretreatment gs rates provided they were rewatered immediately upon reaching the CWP. Spray applications of s-ABA can increase shelf life of select woody ornamentals.

scholarly journals Easy and Efficient DNA Extraction from Woody Plants for the Detection of Phytoplasmas by Polymerase Chain Reaction

Plant Disease ◽  
1999 ◽  
Vol 83 (5) ◽  
pp. 482-485 ◽  
Author(s):  
Margaret J. Green ◽  
Dan A. Thompson ◽  
Donald J. MacKenzie
Keyword(s):  
Polymerase Chain Reaction ◽  
Plant Material ◽  
Woody Plant ◽  
Leaf Roll ◽  
Chain Reaction ◽  
Efficient Procedure ◽  
Identical Manner ◽  
Total Dna ◽  
Polymerase Chain ◽  
Selection Of

A simple and efficient procedure for the extraction of high-quality DNA from phytoplasma-infected woody and herbaceous plants for polymerase chain reaction (PCR) detection is described. This procedure does not require phenol, chloroform, or alcohol for the precipitation of nucleic acids. Herbaceous and woody plant material are extracted in an identical manner with no additional purification or enrichment steps required. The method utilizes commercially available microspin-column matrices, and the extraction of total DNA can be achieved in less than 1 h. The method has been used to successfully purify phytoplasma DNA from whole leaves, leaf petioles and midribs, roots, and dormant wood from a diverse selection of plant material. The phytoplasmas detected by PCR include pear decline, western X-disease, peach yellow leaf roll, peach rosette, apple proliferation, Australian grapevine yellows, and Vaccinium witches'-broom.

Period of harvest, sprouting ability of cuttings, and in vitro plant regeneration in Fraxinus excelsior

1994 ◽  
Vol 72 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Conceição Eneida Silveira ◽  
Alain Cottignies
Keyword(s):  
Chromosome Number ◽  
In Vitro Culture ◽  
Adventitious Roots ◽  
Woody Plant ◽  
Fraxinus Excelsior ◽  
Stem Cuttings ◽  
Natural Conditions ◽  
Apical Bud ◽  
Woody Plant Medium

Propagation by stem cuttings and in vitro culture of apical bud explants were studied on Fraxinus excelsior L. Stem cuttings from 4- to 7-year-old trees growing under natural conditions sprouted only when cuttings were taken from dormant material. Only 6% of those that had sprouted developed roots by the 7th month of culture. Similarly, only apical bud explants harvested during the dormant period sprouted in vitro. Up to 87% of these sprouts developed two to four branching adventitious roots after 5 months of culture. During the initial phase of in vitro culture, the Quoirin and Lepoivre medium and the woody plant medium favoured sprout lengthening. During the phase of multiplication, up to three sprouts per explant developed with the woody plant medium in the presence of a combination of high 6-benzylaminopurine (3.0–4.0 mg∙L−1) and low indole-3-butyric acid (0.01–0.03 mg∙L−1) concentrations. Rooting was obtained in a medium without any growth regulators. Microscopic analysis showed a direct connection between the vascular elements of adventitious roots and stem of plantlet. Chromosome number in root apices of ash plantlets and ash trees grown under natural conditions was 2n = 46. Key words: chromosome number, Fraxinus excelsior L., in vitro plants, micropropagation, stem cuttings.

In Vitro Growth and Sporulation of Blastomyces dermatitidis on Woody Plant Material

Mycologia ◽  
1977 ◽  
Vol 69 (6) ◽  
pp. 1193 ◽  
Author(s):  
D. M. Dixon ◽  
H. Jean Shadomy ◽  
S. Shadomy
Keyword(s):  
Plant Material ◽  
Woody Plant ◽  
Blastomyces Dermatitidis ◽  
In Vitro Growth

scholarly journals Comparison of Perlite and Peat:perlite Rooting Media for Rooting Softwood Stem Cuttings in a Subirrigation System with Minimal Mist

1999 ◽  
Vol 17 (3) ◽  
pp. 147-151
Author(s):  
Gregory J. Giroux ◽  
Brian K. Maynard ◽  
William A. Johnson
Keyword(s):  
Woody Plant ◽  
Sweet Pepper ◽  
Vaccinium Corymbosum ◽  
Stem Cuttings ◽  
Medium Ph ◽  
Rooted Cutting ◽  
Rooting Medium ◽  
Rooting Media ◽  
The Media ◽  
Acer Ginnala

Abstract Perlite and mixtures of milled sphagnum peat and perlite (1:16, 1:8, and 1:4 by vol) were compared as rooting media in a propagation system utilizing subirrigation with minimal mist for rooting softwood stem cuttings of nine woody plant species. The influence of rooting medium on percentage rooting, number of roots per rooted cutting, and length of the longest roots on each cutting varied with species. Composition of the media had no effect on percentage of cuttings that rooted, number of roots per rooted cutting, or length of the longest root of ‘Late Blue’ highbush blueberry (Vaccinium corymbosum L. ‘Late Blue’). Cuttings of Amur maple (Acer ginnala Maxim.) and ‘Ruby Spice’ sweet pepper bush (Clethra alnifolia L. ‘Ruby Spice’) produced the longest root in 1:4 peat:perlite, and cuttings of ‘Ruby Spice’ sweet pepper bush produced the greatest number of roots in 1:8 peat: perlite. Cuttings of redvein enkianthus [Enkianthus campanulatus (Miq.) Nichols] produced more roots in 1:8 peat:perlite than in perlite, and longer roots in 1:16 and 1:8 peat: perlite than in perlite. The number of roots per cutting of ginkgo (Ginkgo biloba L.) was greatest in 1:16 peat: perlite. Cuttings of Carolina silverbell (Halesia carolina L.) rooted in the greatest percentage in perlite, and cuttings of panicle hydrangea (Hydrangea paniculata Sieb.) rooted in the greatest percentages in perlite and 1:8 peat: perlite. The number of roots per cutting of ‘Kwanzan’ cherry and ‘Shasta’ doublefile viburnum [Viburnum plicatum Thunb.var. tomentosum (Thunb.) Rehd. ‘Shasta’] was greatest in 1:16 and 1:8 peat: perlite. Root length per cutting of ‘Kwanzan’ cherry (Prunus serrulata Lindl. ‘Kwanzan’) increased with an increase in the amount of peat in the medium. Rooting medium pH decreased and moisture content increased as the amount of peat in the media increased from 1:16 to 1:8 to 1:4 (peat:perlite, by vol).

scholarly journals Effects of Auxin Concentration and Medium Temperature on Four Woody Plant Taxa Propagated by Stem Cuttings using Recirculating Subirrigation in a Growth Chamber

2003 ◽  
Vol 21 (3) ◽  
pp. 126-130
Author(s):  
James S. Owen ◽  
William A. Johnson ◽  
Brian K. Maynard
Keyword(s):  
Root Formation ◽  
Woody Plant ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Root Number ◽  
Medium Temperature ◽  
Root Numbers ◽  
Auxin Treatment ◽  
Auxin Concentration ◽  
Propagation Medium

Abstract Four woody plant taxa (‘Sparkleberry’ holly, ‘Mariesii’ viburnum, ‘Shasta’ viburnum, and Red Sunset® maple) were propagated from softwood stem cuttings in a recirculating subirrigation propagation system to evaluate the effect on adventitious root formation of three auxin (Dip-n-Grow®) concentrations [0 (water), 20: 1, or 10: 1] and three medium temperatures [20C (68F), 23C (74F), or 26C (79F)]. All taxa showed a positive rooting response to auxin treatment. Compared to cuttings treated with water, root number of all taxa doubled when treated with the highest auxin concentration (10: 1 Dip-n-Grow® dilution). Length of the longest root of rooted cuttings increased with auxin treatment of two taxa. Medium temperature also affected rooting of all taxa. The intermediate temperature evaluated, 23C (74F), was optimal for all rooting parameters on cuttings of ‘Mariesii’ viburnum, and for root length and percentage rooting on cuttings of ‘Shasta’ viburnum and Red Sunset® maple. A propagation medium temperature of 26C (79F) resulted in the greatest root number on cuttings of ‘Shasta’ viburnum, while 20C (68F) was optimal for root number on cuttings of Red Sunset® maple. Root numbers on cuttings of ‘Mariesii’ viburnum were reduced at 20C (68F) and 26C (79F), which appeared to be sub-and supra-optimal temperatures, respectively, for root initiation. All rooting parameters on cuttings of ‘Sparkleberry’ holly increased with propagation medium temperature. Temperature-auxin interactions were apparent for root number and length on cuttings of ‘Mariesii’ and ‘Shasta’ viburnum. The recirculating subirrigation system was useful for evaluating the basal temperature and auxin dose interactions and was effective for rooting softwood stem cuttings without intermittent mist irrigation.

scholarly journals Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants

2012 ◽  
Vol 65 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Ewa Hanus-Fajerska ◽  
Alina Wiszniewska ◽  
Przemysław Czaicki
Keyword(s):  
Plant Material ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Mineral Salts ◽  
Shrub Species ◽  
Multiplication Coefficient ◽  
Shoot Propagation ◽  
Propagation Media

In this study, an attempt was made to investigate <i>in vitro</i> morphogenetic competence of three shrub species from the <i>Thymelaeaceae</i> family. The studied plant material originated from Russia, Greece and China, and the effectiveness of <i>in vitro</i> shoot formation and rhizogenesis of <i>Daphne caucasica</i>, <i>D. jasminea</i>, and <i>D. tangutica</i> was verified. The multiplication coefficient was compared for different propagation media. Medium composed of WPM mineral salts, MS microelements and a set of vitamins, supplemented with 1.0 mg dm<sup>-3</sup> 2iP, 0.1 mg dm<sup>-3</sup> NAA, and 0.65 g dm<sup>-3</sup> calcium gluconate, was appropriate for micropropagation of the tested genotypes. Shoot propagation in medium containing B<sub>5</sub> vitamins and microelements was not as effective as on WPM/MS medium. The rooting phase, especially in <i>D. tangutica</i>, needs further optimization in order to reduce the costs associated with acclimatization of microplantlets obtained to <i>in vivo</i> conditions. After stabilization, the plants were successfully cultivated under greenhouse conditions.

On the role of the light regime in root development of Euphorbia pulcherrima leafy stem cuttings

2016 ◽  
Vol 81 (3) ◽  
pp. 148-151 ◽  
Author(s):  
T. Schwend ◽  
◽  
M. Kriedel ◽  
D. Prucker ◽  
S. Peisl ◽  
...  
Keyword(s):  
Root Development ◽  
Light Regime ◽  
Euphorbia Pulcherrima ◽  
Stem Cuttings

scholarly journals Influence of fertilization application and growing substrate on container-grown woody ornamentals

2011 ◽  
Vol 48 (No. 10) ◽  
pp. 448-457
Author(s):  
F. Šrámek ◽  
M. Dubský
Keyword(s):  
Controlled Release ◽  
Woody Plant ◽  
Slow Release ◽  
Thuja Occidentalis ◽  
Woody Ornamentals ◽  
Slow Release Fertilizer ◽  
Liquid Feeding ◽  
Wood Fibres ◽  
Pyracantha Coccinea ◽  
Additional Nitrogen

Five fertilization systems and seven types of growing substrates were tested with two woody plant species with different nutritional demands (Thuja occidentalis, Pyracantha coccinea) grown in two-litre containers. The experiment was repeated in two vegetative seasons. Application of controlled release fertilizer (CRF) was proved the most reliable; it gave good or at least average results in dependence on used growing substrates. Significant differences between application of CRF and other fertilization systems were with Pyracantha plants. System using slow release fertilizer with additional nitrogen fertilizing also showed good results. Systems based only on liquid feeding were less reliable, with some substrates they showed very good results; with others (peat substrate, peat bark substrates with wood components) they were bad. Similar results were achieved with top dressing of granulated fertilizer. Evaluating the substrates good results were obtained by using mixtures of peat with components with higher content of nutrients &ndash; peat bark substrates, peat bark substrates with compost and non-peat mixtures of composted bark, wood fibres and compost. Significant differences between these types of substrates and peat one or peat bark substrate with wood components were both with Thuja plants and especially with Pyracantha plants, which have higher demand for nutrients. The experiments showed that peat based substrates amended with alternative components or non-peat substrates can bring better results than the peat ones.

scholarly journals PERTUMBUHAN STEK BATANG GMELINA ARBOREA HASIL KOLEKSI DARI LIMA POPULASI SEBARAN DI INDONESIA

2020 ◽  
Vol 14 (2) ◽  
pp. 83-91
Author(s):  
Hamdan Adma Adinugraha ◽  
◽  
Dedi Setiadi ◽  
Arnoldus Naibini ◽  
Nyuwito Nyuwito ◽  
...  
Keyword(s):  
Plant Material ◽  
Small Diameter ◽  
Size Factor ◽  
Stem Cuttings ◽  
Gmelina Arborea ◽  
Diameter Class ◽  
Pattern Design ◽  
Survival Percentage ◽  
Two Factors ◽  
Growth Ability

his studywas conducted to determine the effect of plant material origin and size of cuttings on the growth ability of Gmelina. arborea stem cuttings in the nursery. The study was arranged in a randomized factorial pattern design, consisting of two factors, namely the length of the cuttings (L1 = 20 cm and L2 = 30 cm) and the cuttings size factor (small diameter class/D1 = <1.25 cm, medium/D2 = 1.25 –2.50 cm and large/D3 => 2.50 cm). Each treatment used 5 stem cuttings and repeated in 3 times, which are grouped based on their origin population namely Bantul/P1, Bogor/P2, Lampung/P3,Bondowoso/P4 and Lombok/P5. The observations showed the survival percentage was 83.12% and the rooting percentage was 78.23% which was influenced by the length and diameter of the stem cuttings. The growth of shoots (number, length and diameter) and the number of shoot nodes and leaf were affected by the interaction of origin of the population and the length and diameter of the stem cuttings.Applying stem cutting method is very potential to produce planting stock of Gmelina arbore. Stem cuttings which has a biger size showed better grow of stem cuttings.

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