scholarly journals Isotopic and Nonisotopic Estimation of Nitrogen Uptake Efficiency in Container-grown Woody Ornamentals

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 665-669 ◽  
Author(s):  
David R. Sandrock ◽  
Timothy L. Righetti ◽  
Anita N. Azarenko
Keyword(s):  
N Uptake ◽  
Regression Line ◽  
Dry Weight ◽  
Fertilizer N ◽  
Tracer Method ◽  
Total N ◽  
Uptake Efficiency ◽  
N Uptake Efficiency ◽  
Total Plant ◽  
Fertilizer N Uptake

Cornus sericea L., Weigela florida (Bunge) A. DC., and Euonymus alatus (Thunb.) Sieb were grown outside in 3.8-L plastic containers for 345 days (1 Apr. 2001 to 11 Mar. 2002). Nitrogen (N) was applied at rates (NAR) of 25, 50, 100, 200, and 300 mg·L–1 and delivered as aqueous double-labeled 15N depleted NH4NO3 (min 99.95% atom 14N). In all species, root, shoot, and total plant dry weight increased with increasing NARs while root to shoot ratios decreased. Similarly, root, shoot, and total plant N increased with NAR for each species, and at each NAR more N was stored in the roots than in the shoots. Estimation of fertilizer N uptake determined by the total N method was higher for all species and at each NAR than estimation of N uptake determined by the fertilizer 15N tracer method. Fertilizer N uptake efficiency determined by the total N method was highest at 25 mg·L–1 and decreased as NARs increased. In contrast fertilizer N uptake efficiency determined by the fertilizer 15N tracer method was lowest at 25 mg·L–1 and increased or remained relatively constant as NARs increased. Differences in N uptake and N uptake efficiency can be attributed to overestimation by the total N method due to the inclusion of nonfertilizer N and underestimation by the fertilizer 15N tracer method due to pool substitution. Corrected N uptake efficiency values can be calculated by adjusting the original data (total N or 15N uptake) by the distance between the origin and the y intercept of the regression line representing the data.

The course of fertilizer nitrogen uptake by rainfed sugarcane in Mauritius

1994 ◽  
Vol 122 (3) ◽  
pp. 385-391 ◽  
Author(s):  
K. F. Ng Kee Kwong ◽  
J. Deville
Keyword(s):  
Dry Matter ◽  
N Uptake ◽  
N Recovery ◽  
Dry Matter Accumulation ◽  
Fertilizer N ◽  
N Losses ◽  
Total N ◽  
Fertilizer N Recovery ◽  
The Difference ◽  
Fertilizer N Uptake

SUMMARYThe patterns of N uptake and dry matter synthesis by sugarcane (Saccharum hybrid spp.) were studied at four locations in Mauritius with 15N–labelled ammonium sulphate (100 kg N/ha) applied either in a single dressing in September or in two split applications in September and the following February. More than 80% of the total N recovered at harvest (100–120 kgN/ha) was absorbed by the sugarcane during an active uptake period from October to January. Split application prolonged this active N uptake until April only and had no effect on dry matter accumulation. While total Nabsorbed by above-ground sugarcane showed no decline over time, 10–20 kg N/ha of the 15N–labelled N was lost from the green tops even when the N was applied on two occasions. The fertilizer N losses from above-ground sugarcane were, however, not evident when fertilizer N recovery with time was studied by the difference method. In view of the observed losses of fertilizer N from the aerial parts of sugarcane, measurement of fertilizer N recovery at harvest by the N isotope dilution technique underestimates fertilizer N uptake by sugarcane and attributes too large a fraction of N loss to denitrification/volatilization of NH3.

scholarly journals Uptake, Partitioning, and Storage of Fertilizer Nitrogen in Red Raspberry as Affected by Rate and Timing of Application

2004 ◽  
Vol 129 (3) ◽  
pp. 439-448 ◽  
Author(s):  
Hannah G. Rempel ◽  
Bernadine C. Strik ◽  
Timothy L. Righetti
Keyword(s):  
N Uptake ◽  
Dry Weight ◽  
Rubus Idaeus ◽  
Split Application ◽  
Fertilizer N ◽  
Red Raspberry ◽  
N Content ◽  
Total N ◽  
N Treatment ◽  
Fruit Harvest

The effects of 15N-labeled fertilizer applied to mature summer-bearing red raspberry (Rubus idaeus L. `Meeker') plants were measured over 2 years. Four nitrogen (N) treatments were applied: singularly at 0, 40, or 80 kg·ha-1 of N in early spring (budbreak), or split with 40 kg·ha-1 of N (unlabeled) applied at budbreak and 40 kg·ha-1 of N (15N-depleted) applied eight weeks later. Plants were sampled six times per year to determine N and 15N content in the plant components throughout the growing season. Soil also was sampled seven times per year to determine inorganic N concentrations within the four treatments as well as in a bare soil plot. There was a tendency for the unfertilized treatment to have the lowest and for the split-N treatment to have the highest yield in both years. N application had no significant effect on plant dry weight or total N content in either year. Dry weight accumulation was 5.5 t·ha-1 and total N accumulation was 88 to 96 kg·ha-1 for aboveground biomass in the fertilized plots in 2001. Of the total N present, averaged over 2 years, 17% was removed in prunings, 12% was lost through primocane leaf senescence, 13% was removed through fruit harvest, 30% remained in the over-wintering plant, and 28% was considered lost or transported to the roots. Peak fertilizer N-uptake occurred by July for the single N applications and by September for the last application in the split-N treatment. This uptake accounted for 36% to 37% (single applications) and 24% (last half of split application) of the 15N applied. Plants receiving the highest single rate of fertilizer took up more fertilizer N while plants receiving the lower rate took up more N from the soil and from storage tissues. By midharvest, fertilizer N was found primarily in the fruit, fruiting laterals, and primocanes (94%) for all fertilized treatments; however, the majority of the fertilizer N applied in the last half of the split application was located in the primocanes (60%). Stored fertilizer N distribution was similar in all fertilized treatments. By the end of the second year, 5% to 12% of the fertilizer acquired in 2001 remained in the fertilized plants. Soil nitrate concentrations increased after fertilization to 78.5 g·m-3, and declined to an average of 35.6 g·m-3 by fruit harvest. Seasonal soil N decline was partially attributed to plant uptake; however, leaching and immobilization into the organic fraction may also have contributed to the decline.

scholarly journals Hydrophilic Gel Amendments to Sand Soil Can Increase Growth and Nitrogen Uptake Efficiency of Citrus Seedlings

HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 267-271 ◽  
Author(s):  
J.P. Syvertsen ◽  
J.M. Dunlop
Keyword(s):  
Water Use ◽  
Seedling Growth ◽  
N Uptake ◽  
Poncirus Trifoliata ◽  
N Leaching ◽  
Sand Soil ◽  
N Losses ◽  
Total N ◽  
Uptake Efficiency ◽  
N Uptake Efficiency

We tested the hypothesis that amendments of two hydrophilic gels to a sand soil would reduce N leaching losses and increase growth of citrus seedlings. Three-month-old seedlings of `Swingle' citrumelo [Citrus paradisi Macf. × Poncirus trifoliata (L.) Raf.] were transplanted into containers of steam-sterilized Candler sand, amended with a linear acrylamide/acrylate copolymer (PAM), and/or a cross-linked copolymer agronomic gel (AGRO). Two rates of each amendment were applied either alone or together and were either mixed into dry sand prior to seedling transplant, used as a root-dip slurry at transplant or applied to the soil surface in a solution after transplant. Seedlings were grown in the greenhouse for 5 months and irrigated to container capacity with a dilute nutrient solution without leaching. Pots were leached every 2 weeks and total N losses from the soil were measured in the leachate. PAM amendments increased N retention in soil slightly but PAM had no effect on plant growth, water use, N uptake, or N leaching relative to unamended control plants. The AGRO amendments increased seedling growth, plant water use and uptake of N from 11% to 45% above that of the unamended control plants depending on application method. AGRO decreased N concentrations in the leachate to as low as 1 to 6 mg·L-1. Only 6% of the total applied N was leached from the AGRO treatments, which was about half that from the untreated control plants. There was no additional benefit of using both amendments together or of an additional AGRO root dip treatment. The largest plants used the most water, required the most N and had the greatest N uptake efficiency. AGRO amendments clearly enhanced seedling growth, increased their N uptake efficiency, and reduced N losses from this sand soil.

scholarly journals Isotopic and Nonisotopic Estimation of Nitrogen Fertilizer Uptake in Container-grown Woody Ornamentals

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 891E-892
Author(s):  
David R. Sandrock* ◽  
Timothy L. Righetti ◽  
Anita N. Azarenko
Keyword(s):  
N Uptake ◽  
N Recovery ◽  
Fertilizer N ◽  
Total N ◽  
Euonymus Alatus ◽  
Fertilizer N Recovery ◽  
Isotopic Methods ◽  
The Difference ◽  
Weigela Florida ◽  
Fertilizer N Uptake

Accurate methods for determining the fate and recovery of nitrogen (N) fertilizer applied to container-grown nursery crops are essential to comply with regulations and develop innovative fertilizer programs. The objectives of this study were (i) to use 15N techniques to determine the fate of fertilizer N, (ii) to compare nonisotopic and isotopic methods of determining N recovery, and (iii) to determine the relative importance of fertilizer and non-fertilizer N at rates of 25, 50, 100, 200, and 300 mg·L-1 in container-grown Euonymus alatus (Thunb.) Sieb., Cornus sericea L., and Weigela florida (Bunge) A. DC. In all species, root and shoot N increased with N rate, and at each rate more N was stored in the roots than in the shoots. Estimation of N recovery determined by the total N method (Kjeldahl N/applied N) was significantly higher for all species and at each N rate than estimation of N recovery determined by the labeled fertilizer N method (labeled N/total applied N). Increasing fertilizer rates up to 100 mg·L-1 resulted in increased uptake of N derived from other sources (NDFO). NDFO at low N concentrations was a significant portion of the total N in the plant. As a result, the difference in estimation of percent N recovery between each method was larger at lower N concentrations for all species. The nonisotopic total N method produces higher fertilizer N uptake estimates, as much as three to four times the isotopic based estimates, in container-grown plants at N concentrations of 25 mg·L-1. Actual fertilizer N loss increases dramatically from 25 to 300 mg·L-1 (due to dramatic increases in N applied), despite small gains in fertilizer N recovery efficiency.

scholarly journals Spring Nitrogen Uptake, Use Efficiency, and Partitioning for Growth in Iris germanica ‘Immortality’

HortScience ◽  
2016 ◽  
Vol 51 (5) ◽  
pp. 563-566 ◽  
Author(s):  
Xiaojie Zhao ◽  
Guihong Bi ◽  
Richard L. Harkess ◽  
Jac J. Varco ◽  
Eugene K. Blythe
Keyword(s):  
Nitrogen Uptake ◽  
Leaf Growth ◽  
N Uptake ◽  
Dry Weight ◽  
N Content ◽  
Total N ◽  
Nitrogen Uptake Efficiency ◽  
Iris Germanica ◽  
N Derived From Fertilizer ◽  
Total Plant

This study investigated how spring nitrogen (N) application affects N uptake and growth performance in tall bearded (TB) iris ‘Immortality’ (Iris germanica L.). Container-grown iris plants were treated with 0, 5, 10, 15, or 20 mm N from 15NH415NO3 through fertigation using a modified Hoagland’s solution twice a week for 6 weeks in Spring 2013. Increasing N rate increased plant height, total plant dry weight (DW), and N content. Total N content was closely related to total plant DW. The allocation of N to different tissues followed a similar trend as the allocation of DW. In leaves, roots, and rhizomes, increasing N rate increased N uptake and decreased carbon (C) to N ratio (C/N ratio). Leaves were the major sink for N derived from fertilizer (NDFF). As N supply increased, DW accumulation in leaves increased, whereas DW accumulation in roots and rhizomes was unchanged. This indicates increasing N rate contributed more to leaf growth in spring. Nitrogen uptake efficiency (NupE) had a quadratic relationship with increasing N rate and was highest in the 10 mm N treatment, which indicates 10 mm was the optimal N rate for improving NupE in this study.

Genotypic Variation in C and N Isotope Discrimination Suggests Local Adaptation of Heart-Leaved Willow

2021 ◽  
Author(s):  
Yi Hu ◽  
Robert D Guy ◽  
Raju Y Soolanayakanahally
Keyword(s):  
Isotopic Composition ◽  
Local Adaptation ◽  
N Uptake ◽  
Genotypic Variation ◽  
Carbon Isotopic Composition ◽  
Genotypic Differences ◽  
Clinal Variation ◽  
Trade Off ◽  
Uptake Efficiency ◽  
N Uptake Efficiency

Abstract Plants acquire multiple resources from the environment and may need to adjust and/or balance their respective resource-use efficiencies to maximize grow and survival, in a locally adaptive manner. In this study, tissue and whole-plant carbon isotopic composition (δ13C) and C/N ratios provided long-term measures of use efficiencies for water (WUE) and nitrogen (NUE), and a nitrogen isotopic composition (δ15N) based mass balance model was used to estimate traits related to N uptake and assimilation in heart-leaved willow (Salix eriocephala Michx.). In an initial common garden experiment consisting of 34 populations, we found population level variation in δ13C, C/N and δ15N, indicating different patterns in WUE, NUE and N uptake and assimilation. Although there was no relationship between foliar δ13C and C/N ratios among populations, there was a significant negative correlation between these measures across all individuals, implying a genetic and/or plastic trade-off between WUE and NUE not associated with local adaptation. To eliminate any environmental effect, we grew a subset of 21 genotypes hydroponically with nitrate as the sole N-source, and detected significant variation in δ13C, δ15N and C/N ratios. Variation in δ15N was mainly due to genotypic differences in the nitrate efflux/influx ratio (E/I) at the root. Both experiments suggested clinal variation in δ15N (and thus N uptake efficiency) with latitude of origin, which may relate to water availability and could contribute to global patterns in ecosystem δ15N. There was a tendency for genotypes with higher WUE to come from more water replete sites with shorter and cooler growing seasons. We found that δ13C, C/N, and E/I were not inter-correlated, suggesting that selection of growth, WUE, NUE and N uptake efficiency can occur without trade-off.

scholarly journals Suitability of Sphagnum Moss, Coir, and Douglas Fir Bark as Soilless Substrates for Container Production of Highbush Blueberry

HortScience ◽  
2017 ◽  
Vol 52 (12) ◽  
pp. 1692-1699 ◽  
Author(s):  
Patrick H. Kingston ◽  
Carolyn F. Scagel ◽  
David R. Bryla ◽  
Bernadine Strik
Keyword(s):  
Cocos Nucifera ◽  
Dry Weight ◽  
Douglas Fir ◽  
Sphagnum Moss ◽  
Highbush Blueberry ◽  
Uptake Efficiency ◽  
Lower Porosity ◽  
The Media ◽  
Total Plant ◽  
Container Production

The purpose of the present study was to investigate the suitability of different soilless substrates for container production of highbush blueberry (Vaccinium sp.). Young plants of ‘Snowchaser’ blueberry were grown in 4.4-L pots filled with media containing 10% perlite and varying proportions of sphagnum moss, coconut (Cocos nucifera L.) coir, and douglas fir [Pseudotsuga menziesii Mirb. (Franco)] bark, as well as a commercially available mix of peatmoss, perlite, and other ingredients for comparison. Total plant dry weight (DW) was similar among the treatments at 72 days after transplanting, but at 128 days, total DW was nearly twice as much in the commercial mix and in media with ≥60% peat or coir than in media with ≥60% bark. Inadequate irrigation likely played a role in poor plant growth in bark. Bark had lower porosity and water holding capacity than peat, coir, or the commercial mix and, therefore, dried quickly between irrigations. Bark also reduced plant uptake efficiency of a number of nutrients, including N, P, K, S, Ca, Mg, Mn, B, Cu, and Zn. Uptake efficiency of P, K, and Mg also differed between plants grown in peat and coir, which in most cases was a function of the initial concentration of nutrients in the media. Before planting, peat had the highest concentration of Mg and Fe among the media, whereas coir had the highest concentration of P and K. Leachate pH was initially lowest with peat and highest with coir but was similar among each of the media treatments by the end of the study. Electrical conductivity (EC) of leachate never exceeded 0.84 dS·m−1 in any treatment. Overall, peat and coir appear to be good substrates for container production of highbush blueberry. Bark, on the other hand, was less suitable, particularly when it exceeded 30% of the total media composition.

Evaluation of Maize Inbreds for Vegetative Nitrate Uptake and Assimilation

1989 ◽  
Vol 16 (2) ◽  
pp. 161 ◽  
Author(s):  
RT Weiland
Keyword(s):  
Nitrate Uptake ◽  
N Uptake ◽  
Dry Weight ◽  
Root Surface ◽  
Root Surface Area ◽  
Controlled Environment ◽  
Uptake Rates ◽  
Total Plant ◽  
Maize Inbreds ◽  
Hydroponic Conditions

Twelve maize (Zea mays L.) inbred lines were cultured under hydroponic conditions in a controlled environment room for evaluation of NO3--N uptake, subsequent translocation patterns and utilisation. Prior to harvest (8-10-leaf stage), inbred roots were exposed to 24 h of 10 atom % 15NO3--N. Differences for N contents and biomass were determined. Root dry weight (RDW) was significantly correlated (r = 0.93) with root surface area. Significant inbred differences were found when N content in the plant was based on RDW. When based on 15N during the 24 h, the amount of N absorbed varied between 30 and 71 mg per plant for the inbreds. Uptake rates ranged between 8.7 and 14.4 mg g-1 day-1 RDW. Nitrogen uptake over the 24 h based on RDW and total plant-N contents based on RDW were significantly (P< 0.001) correlated, implying that uptake rates were constant up to mid-vegetative stages for these genotypes. Of the 15N absorbed, between 30 and 61% was reduced by the inbreds and the amount reduced varied with plant tissue. The total amount reduced ranged between 0.72 and 1.25 mg g-1 dry weight.

Performance of nitrate compared with urea fertilizer in a semiarid climate of the northern Great Plains

2019 ◽  
Vol 99 (3) ◽  
pp. 345-355
Author(s):  
Richard E. Engel ◽  
Carlos M. Romero ◽  
Patrick Carr ◽  
Jessica A. Torrion
Keyword(s):  
Grain Yield ◽  
Great Plains ◽  
N Uptake ◽  
Triticum Aestivum L ◽  
Field Trials ◽  
Northern Great Plains ◽  
N Recovery ◽  
Fertilizer N ◽  
Total N ◽  
Semiarid Regions

Fertilizer NO3-N may represent a benefit over NH4-N containing sources in semiarid regions where rainfall is often not sufficient to leach fertilizer-N out of crop rooting zones, denitrification concerns are not great, and when NH3 volatilization concerns exist. The objective of our study was to contrast plant-N derived from fertilizer-15N (15Ndff), fertilizer-15N recovery (F15NR), total N uptake, grain yield, and protein of wheat (Triticum aestivum L.) from spring-applied NaNO3 relative to urea and urea augmented with urease inhibitor N-(n-butyl)thiophosphoric triamide (NBPT). We established six fertilizer-N field trials widespread within the state of Montana between 2012 and 2017. The trials incorporated different experimental designs and 15N-labeled fertilizer-N sources, including NaNO3, NH4NO3, urea, and urea + NBPT. Overall, F15NR and 15Ndff in mature crop biomass were significantly greater for NaNO3 than urea or urea + NBPT (P < 0.05). Crop 15Ndff averaged 53.8%, 43.9%, and 44.7% across locations for NaNO3, urea, and urea + NBPT, respectively. Likewise, crop F15NR averaged 52.2%, 35.8%, and 38.6% for NaNO3, urea, and urea + NBPT, respectively. Soil 15N recovered in the surface layer (0–15 cm) was lower for NaNO3 compared with urea and urea + NBPT. Wheat grain yield and protein were generally not sensitive to improvements in 15Ndff, F15NR, or total N uptake. Our study hypothesis that NaNO3 would result in similar or better performance than urea or urea + NBPT was confirmed. Use of NO3-N fertilizer might be an alternative strategy to mitigate fertilizer-N induced soil acidity in semiarid regions of the northern Great Plains.

scholarly journals Effect of Different Levels of Urea Super Granule and Prilled Urea on the Crop Quality, Nutrient Uptake and Soil Nutrient Status of Broccoli

2018 ◽  
Vol 15 (2) ◽  
pp. 24-39 ◽  
Author(s):  
M J Hussain ◽  
A J M S Karim ◽  
A R M Solaiman ◽  
M S Islam ◽  
M Rahman
Keyword(s):  
Nutrient Uptake ◽  
N Uptake ◽  
Nutrient Status ◽  
Comparative Performance ◽  
Chlorophyll Contents ◽  
Post Harvest ◽  
Uptake Efficiency ◽  
Prilled Urea ◽  
N Uptake Efficiency ◽  
Β Carotene

A field experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during the period 2010-2011 to assess the effect of urea super granule (USG) and prilled urea (PU) on the quality attributes (Compactness coefficient, Vitamin C, β-carotene and chlorophyll contents) of broccoli, to assess the comparative performance of USG and PU on nutrient uptake and uptake efficiency of broccoli plant, to assess the effect of different forms and levels of urea N on the post harvest soil nutrient status of broccoli field and to select the better doses of USG and PU for quality broccoli production in Shallow Red-Brown Terrace Soil under Madhupur Tract (AEZ-28). The experiment was laid out in a randomized complete block design with three replications having 17 treatments constituted with different levels of urea super granule (USG) and prilled urea (PU) as- T1: N-control,  T2: PU-N80, T3: PU-N100, T4: PU-N120, T5: PU-N140, T6: PU-N160, T7: PU-N180, T8: PU-N200, T9: PU-N220, T10: USG-N80, T11: USG-N100,  T12: USG-N120, T13: USG-N140, T14: USG-N160, T15: USG-N180, T16: USG-N200, T17 and USG-N220 kg ha-1. Results revealed that the comparative performance of USG in relation to head quality (compactness coefficient, ascorbic acid, β-carotene and chlorophyll content), nutrient (NPKS) uptake and N uptake efficiency was found better as compared to PU. The compactness coefficient, β-carotene and chlorophyll contents were increased with increasing rate of N as well as USG and PU, but ascorbic acid content was slightly decreased with increasing rate of USG and PU. The maximum compactness coefficient (18.61) was found from the treatment USG-N180 that was followed by USG-N160 (18.24) and the highest β-carotene content (0.401 mg/100gFW) was found from USG-N160 followed by PU-N180 (0.40 mg/100gFW). Similarly the highest chlorophyll-a content (0.818 mg/100gFW) was found from USG-N180 followed by USG-N160 (0.814 mg/100gFW) and the highest chlorophyll-b content (1.141mg/100g FW) was recorded from USG-N180. The higher nutrient uptake and N uptake efficiency (108.531%) was obtained from USG treated plots over that of the PU. But N uptake efficiency was decreased with increasing rates of N fertilizer. But post harvest soil nitrogen status did not show any systematic trend although it was found higher in USG over PU. Similarly no remarkable changes were observed in post harvest soil P, K, S and B status for the crop. However, USG @ 160 kg N ha-1 (USG-N160) followed by USG-N140 and PU @ 180 kg N ha-1 (PU-N180) with other recommended doses of fertilizer could be suggested as USG and PU based fertilizer recommendation for a good quality broccoli production in terms of yield and quality in Silty Clay Loam Soil of Madhupur Tract.The Agriculturists 2017; 15(2) 24-39 

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