Iron-[S,S′]-EDDS (FeEDDS) Chelate as an Iron Source for Horticultural Crop Production: Marigold Growth and Nutrition, Spectral Properties, and Photodegradation

Aminopolycarboxylic acid (APCA) complexones, commonly referred to as ligands or chelating agents, like ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA), are commonly used in soluble fertilizers to supply copper (Cu), iron (Fe), manganese (Mn), and/or zinc (Zn) to plants. Offsite runoff and contamination of surface waters with these chelating agents is of increasing concern as a result of their reported ability to remobilize heavy metals in sediments and their low susceptibility to biodegradation. The APCA ethylenediaminedisuccinic acid (EDDS) is a structural isomer of EDTA with the [S,S′] stereoisomer of the complexone, a compound naturally produced by actinomycetes, and is biodegradable. Information on the use of [S,S′]-EDDS as a chelating agent in formulating soluble fertilizers for the production of horticultural crops is limited. Therefore, a series of studies were conducted with the objectives of evaluating Fe[S,S′]-EDDS as an Fe-chelate fertilizer agent in the production of marigold and [S,S′]-EDDS (free ligand) and/or Fe[S,S′]-EDDS spectral properties and vulnerability to photodegrdation. Marigold grown in peat-based media were fertilized with complete nutrient solution containing 1 mg·L−1 Fe from FeEDDS, FeEDTA, or FeDTPA. There was no significant difference in foliar Fe or Mn between Fe-chelate treatments, averaging 140 μg·g−1 and 88 μg·g−1, respectively, nor were there significant differences in leaf dry weight (2.30 g) between Fe treatments. Spectra of [S,S′]-EDDS and Fe[S,S′]-EDDS produced from ferrous or ferric sources of Fe absorbed maximally in the 210 to 230 nm and 238 to 240-nm range, respectively. The [S,S′]-EDDS complexone used in the current study, a 30% assay solution, had chromaphoric properties, appearing light yellow in color. When exposed to light, Fe[S,S′]-EDDS quickly degraded at a rate at least twice that of FeEDTA.

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The Effects of Micronutirents on Growth and Yield of Lowland Rice Grown on Typic Dystrudept Soil

Journal of Tropical Soils ◽

10.5400/jts.2019.v24i1.1-10 ◽

2019 ◽

Vol 24 (1) ◽

pp. 1

Author(s):

Husnain Husnain ◽

Diah Setyorini ◽

Asmarhansyah Asmarhansyah ◽

Andriati Andriati

Keyword(s):

Plant Height ◽

Crop Production ◽

Block Design ◽

Dry Weight ◽

Lowland Rice ◽

Growth And Yield ◽

Fertilizer Treatment ◽

Npk Fertilizers ◽

Standard Dosage ◽

Significant Difference

Fertilizers and fertilization technology have important roles in increasing crop production including lowland rice. In response to the issue, a field experiment was conducted to study the effects of micronutrients on growth and yield of lowland rice. The experiment was carried out at Cihideung Ilir Village, Dermaga Sub-district, Bogor Regency from November 2015 until March 2016. The experiment consisted of 8 treatments, namely control (without fertilization), NPK-standard, NPK-standard + ¼ dosage of Hortigro Rice fertilizer, and ¾ NPK + ¼ until 11/4 dosages of Hortigro Rice fertilizer as a source of macronutrients N, P, K and micronutrients of Mn, Cu, Zn, B, and Mo. The treatments were arranged in a Randomized Block Design with 3 replications. Parameters observed during the research were plant height, tiller number, and yield of grain and straw of INPARI-32 lowland rice as crop indicator. The results showed that among other treatments, the highest plant height was resulted from the application of ¾ NPK-standard + ¼ Hortigro Rice, i.e. around 124 cm at 60 days after planting. The highest harvesting dry weight of grain and straw were observed in NPK-standard + 1/4 of Hortigro Rice fertilizer treatment, i.e. around 9.0 Mg ha-1 and 25.9 Mg ha-1, respectively. This yield showed a significant difference with ¾ NPK-standard combined with ¾ -11/4 dosages of Hortigro Rice. The highest dry weight of milled grains was resulted in the application of ¾ NPK-standard + 1/2 Hortigro Rice, i.e. around 7.3 Mg ha-1, which is significantly different from that in ¾ NPK-standard combined with 1.0 - 11/4 dosages of Hortigro Rice treatments, while the highest dry weight of straw was provided by NPK-standard, i.e. around 14.0 Mg ha-1. The highest dry weight of 1000 milled grains and ratio of dry weight of milled grain to straw were provided by ¾ NPK-standard + 1/2 Hortigro Rice around 32.0 gram and 0.63, respectively. The results indicated that the application of micronutrient fertilizer of Hortigro Rice in combination with NPK fertilizers can potentially increase lowland rice productivity due to the micronutrients content of Mn, Cu, Zn, B, and Mo in it.

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Evaluation of the Action of Chelating Agents on the Microhardness of Superficial Layer of Root Canal Lumen Dentin

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1272 ◽

2014 ◽

Vol 5 (2) ◽

pp. 124-128

Author(s):

Srinidhi Surya Raghavendra ◽

Bandu Devrao Napte

Keyword(s):

Root Canal ◽

Chelating Agents ◽

Chelating Agent ◽

Superficial Layer ◽

Time Data ◽

Significance Level ◽

Multiple Comparison Test ◽

Significant Difference ◽

Post Hoc ◽

Better Than

ABSTRACT Aim To evaluate the effect of different chelating agents on the microhardness of the superficial dentin layer of the root canal lumen. Materials and methods Forty-two extracted single rooted teeth were instrumented, and the roots were longitudinally sectioned in a buccolingual direction to expose the entire canal extension. The specimens were randomly distributed in four groups according to the final irrigation: Glyde File Prep (Dentsply Maillefer, Ballaigues, Switzerland), RC Help (Prime Dental Pvt. Ltd, Mumbai, India), Endo Prep RC (Stedman Anabond, Chennai, India), Dolo Endogel (Prevest Denpro Ltd, Jammu, India). A standardized volume of 50 ml of each chelating agent was used for 5 minutes. Dentin microhardness was measured with a Knoop indenter under a 50 gm load and a 15 seconds dwell time. Data was analyzed statistically by one-way analysis of variance ANOVA and post hoc multiple-comparison test at 5% significance level. Results Statistically significant difference was detected among the chelating agents by one-way ANOVA. Glyde File Prep and Dolo Endogel chelating agents differed significantly (p < 0.0001) in reduction of dentin microhardness from RC help and Endo Prep RC. Conclusion Glyde File Prep and Dolo Endogel are significantly better than RC Help and Endo Prep RC in reducing dentin microhardness. How to cite this article Napte BD, Srinidhi SR. Evaluation of the Action of Chelating Agents on the Microhardness of Superficial Layer of Root Canal Lumen Dentin. World J Dent 2014;5(2):124-128.

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Enhanced Degradation of Phenol by a Fenton-Like System (Fe/EDTA/H2O2) at Circumneutral pH

Catalysts ◽

10.3390/catal9050474 ◽

2019 ◽

Vol 9 (5) ◽

pp. 474 ◽

Cited By ~ 5

Author(s):

Selamawit Ashagre Messele ◽

Christophe Bengoa ◽

Frank Erich Stüber ◽

Jaume Giralt ◽

Agustí Fortuny ◽

...

Keyword(s):

Chelating Agents ◽

Ethylenediaminetetraacetic Acid ◽

Chelating Agent ◽

Nitrilotriacetic Acid ◽

Molar Ratio ◽

Phenol Removal ◽

Stoichiometric Ratio ◽

Initial Ph ◽

Phenol Conversion ◽

Circumneutral Ph

This work deals with the degradation of phenol based on the classical Fenton process, which is enhanced by the presence of chelating agents. Several iron-chelating agents such as ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), diethylenetriamine pentaacetic acid (DTPA), and ethylenediamine-N,N’-diacetic acid (EDDA) were explored, although particular attention was given to EDTA. The effect of the molar ligand to iron ratio, EDTA:Fe, initial pH, and temperature on the oxidation process was studied. The results demonstrate that the proposed alternative approach allows the capacity for degrading phenol to be extended from the usual acidic pH (around 3.0) to circumneutral pH range (6.5–7.5). The overall feasibility of the process depends on the concentration of the chelating agent and the initial pH of the solution. The maximum phenol conversion, over 95%, is achieved using a 0.3 to 1 molar ratio of EDTA:Fe, stoichiometric ratio of H2O2 at an initial pH of 7.0, and a temperature of 30 °C after 2 hours of reaction, whereas only 10% of phenol conversion is obtained without EDTA. However, in excess of ligand (EDTA:Fe > 1), the generation of radicals seems to be strongly suppressed. Improvement of the phenol removal efficiency at neutral pH also occurs for the other chelating agents tested.

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Evaluation of Rock Mechanical Properties Alteration During Matrix Stimulation With Chelating Agents

Journal of Energy Resources Technology ◽

10.1115/1.4032546 ◽

2016 ◽

Vol 138 (3) ◽

Cited By ~ 8

Author(s):

Assad Barri ◽

Mohamed Mahmoud ◽

Salaheldin Elkatatny

Keyword(s):

Mechanical Properties ◽

Elastic Properties ◽

Carbonate Rocks ◽

Chelating Agents ◽

Ethylenediaminetetraacetic Acid ◽

Chelating Agent ◽

Well Stimulation ◽

Before And After ◽

Rock Mechanical Properties ◽

Carbonate Formations

Well stimulation using acidic solutions is widely used to treat carbonate formations. The acidic fluids remove the near-wellbore damage and create channels around the wellbore by dissolving fraction of the carbonate rocks. Many stimulation fluids have been used such as hydrochloric acid (HCl) acid, organic acids, and chelating agents to stimulate carbonate reservoirs. Wormholes that are created by these fluids are very effective and will yield negative skin values and this will enhance the well productivity. In addition to the wormhole creation, the diffusion of these fluids inside the pores of the rock may create significant and permanent changes in the rock mechanical properties. These changes can eventually lead to weakening the rock strength, which may lead to future formation damage due to the wellbore instability. In this paper, the effect of ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA) chelating agents on the carbonate rocks elastic properties was investigated. The effect of wormholes created by chelating agent on the rock mechanical properties was investigated. Computed tomography (CT) scan and acoustic measurements were conducted on the core samples before and after matrix stimulation treatments. Experimental results showed that the mechanical properties of strong rocks such as Indiana limestone (IL) cores were not affected when chelating agents were used to stimulate those cores. On the other hand, less strong rocks such as Austin chalk (AC) show significant alteration on the rock elastic properties when chelating agents were used as stimulation fluids.

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Effects of FeEDDS and EDDS on Peat-based Substrate pH and Cu, Fe, Mn, and Zn Solubility

HortScience ◽

10.21273/hortsci.47.2.269 ◽

2012 ◽

Vol 47 (2) ◽

pp. 269-274 ◽

Cited By ~ 1

Author(s):

Joseph P. Albano

Keyword(s):

Crop Production ◽

Chelating Agents ◽

Ethylenediaminetetraacetic Acid ◽

Deionized Water ◽

Chelate Ligand ◽

Solution Ph ◽

Horticultural Crop ◽

Iron Source ◽

Extractant Solution ◽

Substrate Ph

Common chelating agents used in horticultural fertilizers like ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), and ethylenediaminedi(o-hydroxyphenylacetic) acid (EDDHA) are not readily biodegradable and may persist in the environment, maintaining the capacity to solubilize heavy metals. For this reason, biodegradable chelating agents like ethylenediaminedisuccinic acid (EDDS) are being evaluated for use in horticultural crop production. Therefore, the objectives of the study were to determine the effects of FeEDDS and EDDS on substrate pH and copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn) solubility in peat-based substrate compared with various Fe and chelate-ligand sources. Extractions were performed using the 1:2 by volume substrate analysis method with an incubation period of 24 hours. The control was distilled deionized water extractions. Iron-source (FS) extractants consisted of 1 mg·L−1 Fe solutions derived from FeEDDS, FeEDTA, FeDTPA, FeEDDHA, and FeSO4. Iron-source extractant solution pH ranged from 7.1 (FeEDDS) to 5.4 (FeSO4). The extract pH for all Fe-source treatments was similar at pH 6.7, demonstrating the buffering capacity of the peat-based substrate. Iron recovery rates for FS treatments were determined after subtracting Fe that was freely extracted with distilled-deionized water: FeSO4 (13%), FeEDDHA (68%), FeEDDS (73%), FeEDTA (102%), and FeDTPA (121%). Iron-source treatments were not different for Mn, averaging 0.03 mg·L−1, and Cu (0.04 mg·L−1) and Zn (0.24 mg·L−1) were greatest in the FeEDDS treatment. Chelate-ligand (CL) extractants consisted of 5 mm solutions of EDDS, EDTA, and DTPA. Chelate-ligand extractant solution pH ranged from 9.7 (EDDS) to 2.3 (DTPA), and extract solution pH ranged from 7.2 (EDDS) to 4.7 (DTPA). Extractant solutions of EDDS and DTPA resulted in the lowest and highest levels of Cu (0.06 and 0.14 mg·L−1, respectively) and Fe (4.3 and 13.1 mg·L−1, respectively) in extract solutions. Overall, these results suggest that there are no negative implications for the use of FeEDDS with peat-based substrate in terms of horticultural crop production based on substrate Fe solubility, which was not different from FeEDTA.

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PENGARUH SP-36 DAN ASAM HUMAT TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN KEDELAI ( Glycine max L)

BUANA SAINS ◽

10.33366/bs.v19i2.1742 ◽

2020 ◽

Vol 19 (2) ◽

pp. 1

Author(s):

Fajar Setyawan ◽

Feri Setyawan

Keyword(s):

Glycine Max ◽

Humic Acid ◽

Crop Production ◽

Sandy Loam ◽

Block Design ◽

Dry Weight ◽

Observation Data ◽

Significance Level ◽

Significant Difference ◽

Glycine Max L

Soybean is a food crop commodity that is needed by the Indonesian population because it is a source of vegetable protein, fat, vitamins, and minerals. To increase soybean production one of the things that needs to be done is the addition of phosphorus to increase the density of soybean seeds. The purpose of this study was to determine the effect of interactions between the administration of SP36 fertilizer doses and humic acid doses on the growth and production of soybean (Glycine max L) Dega 1 variety. Plosoklaten, Kediri Regency, with a height of 105 meters above sea level, with sandy loam soil type with soil pH 5. The study used factorial randomized block design (RBD) consisting of two factors, repeated 3 times. Parameters observed were Plant dried weight, plant growth rate, number of pods/plants, the weight of Seeds per plot, yield, observation data were analyzed using analysis of variance (F test). The results of the analysis of the real variance were continued with the Significant Difference test at a 5% significance level to find out the difference between treatments. The first factor is SP36 fertilizer and the second factor is humic acid. Based on the results of research that has been done, there was an interaction between the administration of SP36 fertilizer and humic acid in the parameters of plant dry weight at the age of 14 and 21, number of pods, seed weight per plot and yield., Giving fertilizer SP36 200 kg /ha with humic acid 50kg / ha can increase crop production by 43% compared to without SP36 and without humic acid.

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Utility of Claudin-3 in extracellular vesicles from human bile as biomarkers of cholangiocarcinoma

Scientific Reports ◽

10.1038/s41598-021-81023-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Chisaki Ikeda ◽

Hiroaki Haga ◽

Naohiko Makino ◽

Tatsutoshi Inuzuka ◽

Ayako Kurimoto ◽

...

Keyword(s):

Extracellular Vesicles ◽

Proteomic Analysis ◽

Chelating Agents ◽

Ethylenediaminetetraacetic Acid ◽

Ethylene Glycol Tetraacetic Acid ◽

Human Bile ◽

Significant Difference ◽

High Concentrations ◽

Size And Morphology ◽

Phosphate Buffered Saline

AbstractExtracellular vesicles (EVs) are released from all cells. Bile directly contacts bile duct tumor; bile-derived EVs may contain high concentrations of cancer biomarkers. We performed a proteomic analysis of human bile-derived EVs and identified a novel biomarker of cholangiocarcinoma (CCA). EVs were isolated using ultracentrifugation, and chelating agents, ethylenediaminetetraacetic acid and ethylene glycol tetraacetic acid (EDEG) and phosphate buffered saline (PBS) were used as dissolution solutions. Bile was collected from 10 CCA and 10 choledocholithiasis (stones) cases. Proteomic analysis was performed; subsequently, ELISA was performed using the candidate biomarkers in a verification cohort. The vesicles isolated from bile had a typical size and morphology. The expression of exosome markers was observed. RNA was more abundant in the EDEG group. The proportion of microRNA was higher in the EDEG group. EDEG use resulted in the removal of more contaminants. Proteomic analysis identified 166 proteins as CCA-specific. ELISA for Claudin-3 revealed statistically significant difference. The diagnostic accuracy was AUC 0.945 and sensitivity and specificity were 87.5%. We report the first use of EDEG in the isolation of EVs from human bile and the proteomic analysis of human bile-derived EV-proteins in CCA. Claudin-3 in bile-derived EVs is a useful biomarker for CCA.

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Effect of planting date and density on calendula and peppermint herbs

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v1i1.4241 ◽

2014 ◽

Vol 1 (1) ◽

pp. 25-29

Author(s):

Rahim Mohammadian ◽

Behnam Tahmasebpour ◽

Peyvand Samimifar

Keyword(s):

Mutual Effect ◽

Dry Weight ◽

Planting Date ◽

Mass Reduction ◽

Flower Number ◽

Planting Dates ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Total Maximum

A factorial experiment was conducted with a completely randomized design to evaluate the effects of planting date and density on calendula herbs and peppermint. It had 3 replicates and was done in Khosroshahr research farm, Tabriz in 2006. Under studied factors were: 3 planting dates (10 May, 25 May and 10 June) in 4 densities (25, 35, 45, 55) of the plant in square meters. The results of variance a nalysis showed that there was 1% probability significant difference between the effects of planting date and bush density on the leave number, bush height and the bush dry weight. But the mutual effect of the plant date in mentioned traits density was insignificant. Regarding the traits mean comparison, the total maximum dry weight was about the 55 bush density in mm. Also, the bush high density in mm causes the bush growth and its mass reduction. When there is the density grain, the flower number will increase due to bush grain in surface unit. Overall, we can conclude that 10 June planting and 45 bush density in mm is the most suitable items and results in favored production with high essence for these crops.

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Assessment of Bacterial Contamination of Orthodontic Arch wire

Journal of Baghdad College of Dentistry ◽

10.26477/jbcd.v31i1.2578 ◽

2019 ◽

Vol 31 (1) ◽

pp. 48-51

Author(s):

Suha S Hassan ◽

Nidhal H. Ghaib ◽

Batool H Al-Ghurabi

Keyword(s):

Bacterial Contamination ◽

Microbial Contamination ◽

Ethylenediaminetetraacetic Acid ◽

Bacterial Species ◽

Brain Heart Infusion ◽

Control Groups ◽

Dental Practitioners ◽

Significant Difference ◽

Bacillus Spp ◽

Droplet Transmission

Background: The microorganisms can impend the life of health care professional and particularly the dental practitioners. They can be transmitted by different ways like airborne and droplet transmission. The current study was carried out to identify whether the arch wires that received from the manufactures are free from microbial contamination and to determine the bacterial species attached to the arch wires. Materials and Methods: This study involved eighty samples, consisted of two types of arch wires (nitinol and stainless-steel) from four companies (3M, G&H, Jiscop, OrthoTechnology). These wires inserted in a plane tube that contains 10 -ml of (Tris [tris(hydroxymethyl)aminomethane] and EDTA (ethylenediaminetetraacetic acid) tris-EDTA and brain heart infusion (BHI) broth. A 0.1 ml was withdrawn from the tube and spread on agar plates. The control groups consist of 16 plane tube (8 tubes with tris-EDTA and other 8 tubes with (BHI). Results: Microbial sampling yielded growth from 5 of the 80 arch wires. The predominant bacteria that isolated were Bacillus spp. No growth was recovered from 75 of the samples and from controls. The bacteria were isolated by BHI reagent and no growth was observed by tris-EDTA reagent with statistically significant difference (P<0.05). The Bacillus spp. found only in the G&H and Jiscop companies, however, no statistically significant difference was found among them (P>0.05). With regard to the presence and distribution of bacteria according to the types of wires, the present results clarified that cases of contamination with Bacillus spp. were found in the nitinol arch wires with statistically significant difference (P<0.05). Conclusions: The results of the current study revealed low count of bacterial contamination in the two types of companies (G&H and Jiscop). Not all materials that received from the manufactures are free from contamination and an effective sterilization regimen is needed to avoid cross-contamination.

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Evaluation of Nurse Crops for Weed Control and Plant Establishment in Prairie Restoration

HortScience ◽

10.21273/hortsci.33.3.485b ◽

1998 ◽

Vol 33 (3) ◽

pp. 485b-485

Author(s):

Lisa M. Barry ◽

Michael N. Dana

Keyword(s):

Plant Biomass ◽

Spring Barley ◽

Dry Weight ◽

Site Quality ◽

Prairie Restoration ◽

Plant Establishment ◽

Significant Difference ◽

Nurse Crops ◽

Partridge Pea ◽

Prairie Plant

Nurse crops are often recommended in prairie restoration planting. This work investigated several alternative nurse crops to determine their utility in prairie planting. Nurse crops were composed of increasing densities (900, 1800, or 2700 seeds/m2) of partridge pea, spring oats, spring barley, Canada wild rye, or equal mixtures of partridge pea and one of the grasses. The experimental design was a randomized complete-block set in two sites with three blocks per site and 48 treatments per block. Each 3 × 3-m plot contained 1 m2 planted in Dec. 1995 or Mar. 1996 with an equal mix of seven prairie species. The nurse crops were sown over each nine square meter area in April 1996. Plots lacking nurse crops served as controls. Evaluated data consisted of weed pressure rankings and weed and prairie plant dry weight. Nurse crop treatments had a significant effect on weed pressure in both sites. Barley (1800 and 2700 seeds/m2) as well as partridge pea + barley (2700 seeds/m2) were most effective at reducing weed pressure. When weed and prairie plant biomass values were compared, a significant difference was observed for site quality and planting season. Prairie plant establishment was significantly greater in the poorly drained, less-fertile site and spring-sown plots in both sites had significantly higher prairie biomass values. Overall, after two seasons, there was no advantage in using nurse crops over the control. Among nurse crop treatments, oats were most effective in reducing weed competition and enhancing prairie plant growth.

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