scholarly journals Rooting Ability of Hardwood Cuttings in Highbush Blueberry (Vaccinium corymbosum L.) Using Different Indole-butyric Acid Concentrations

HortScience ◽  
2018 ◽  
Vol 54 (2) ◽  
pp. 194-199 ◽  
Author(s):  
Haishan An ◽  
Jiajia Meng ◽  
Fangjie Xu ◽  
Shuang Jiang ◽  
Xiaoqing Wang ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Vaccinium Corymbosum ◽  
Control Treatment ◽  
Highbush Blueberry ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Rooting Ability ◽  
Rooting Response ◽  
Main Factor

Vegetative propagation by cuttings is a very popular method. However, blueberry propagation using cuttings is still a main factor limiting its expansion because its results can vary according to the blueberry cultivar and environmental factors. This study aimed to evaluate the rooting abilities of hardwood cuttings for six blueberry cultivars (O’Neal, Misty, Diana, Biloxi, Bluebeauty, and Coville) using three different exogenous indole-butyric acid (IBA) concentrations (1000, 2000, and 3000 ppm), and to determine if the cutting position (basal, central, apical) affects rooting performance. A control treatment (0 ppm IBA) was also performed. After 90 days of each treatment, rooting percentage, average root length, and average root number per cutting were assessed and used to calculate rooting index, which is a measure of rooting ability. The rooting percentages of hardwood cuttings differed largely among cultivars and were highest for ‘Bluebeauty’ (68.55%), followed by ‘Biloxi’ (68.01%). The rooting index values of these two cultivars (33.59 and 35.18, respectively) were significantly higher than those of the other four cultivars. The rooting response of blueberry hardwood cuttings to IBA concentrations was quadratic, and 1000 and 2000 ppm IBA were sufficient to express the maximum rooting percentage in most cultivars. The rooting abilities of basal, central, and apical cuttings were similar with treatments with high IBA concentrations. The effects of the cultivar, IBA concentration, and interaction between them on rooting percentage, average root length, and average root number were significant; however, the effects of the cutting position on the rooting percentage and average root length were not. This suggested that the rooting abilities of blueberry hardwood cuttings were significantly influenced by the cultivar and IBA concentration rather than by the cutting position.

scholarly journals Rooting Response of Stem Cuttings of Shantung Maple (Acer truncatum) to Time of Year, Cutting Position, and Auxin Concentration, Formulation, and Solvent

2014 ◽  
Vol 32 (3) ◽  
pp. 163-166
Author(s):  
Justin A. Brock ◽  
Jason J. Griffin
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Stem Cuttings ◽  
Root Number ◽  
Solvent Water ◽  
Auxin Concentration ◽  
Time Of Year ◽  
Rooting Response

The influence of time of year, cutting position, and auxin concentration, formulation, and solvent on rooting of stem cuttings of shantung maple (Acer truncatum Bunge) was investigated in seven experiments. Softwood, semi-hardwood, second-flush softwood, and hardwood stem-tip cuttings were compared. Semi-hardwood cuttings [15 cm (6 in) in length] rooted best (55%). Auxin treatments [indole-3-butyric acid (IBA) or the potassium (K) salt of IBA (K-IBA)] ranged from 0 to 15,000 ppm (0 to 1.5%). Generally, rooting percentage decreased as auxin concentration increased. Cutting position (terminal or subterminal), auxin formulation (liquid or powder), and auxin solvent [water or ethanol:water (1:1 v/v)] did not affect percent rooting. Mean root number and mean root length were unaffected by all treatments. Results suggest shantung maple roots best from semi-hardwood cuttings treated with auxin at concentrations of 0 or 2,500 ppm (0.25%).

scholarly journals Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of damask roses (Rosa damascena Mill.)

2015 ◽  
Vol 60 (3) ◽  
pp. 263-275 ◽  
Author(s):  
Fardin Nasri ◽  
Arsalan Fadakar ◽  
Mahmood Saba ◽  
Bayzid Yousefi
Keyword(s):  
Butyric Acid ◽  
Control Treatment ◽  
Rosa Damascena ◽  
Indole Butyric Acid ◽  
Significant Difference ◽  
Maximum Root Length ◽  
Number Of Leaves ◽  
Maximum Root ◽  
Medicinal Properties ◽  
The Rose

Rosa damascena is very important for essential oil production, medicinal properties and it is also widely cultivated as a garden rose. The Rose species is mainly propagated by stem cutting. In the present study, the effect of different levels of 0, 500 and 1,000 mg l-1 (quick dip method for 20 s) of indole butyric acid (IBA) on the rooting of 12 wild genotypes (including: Kurdistan 1 to Kurdistan 12) of R. damascena was investigated. The results show that the rooting ability of R. damascena differs significantly between the twelve genotypes. The highest rooting (79.56%) and callus production (69.08%), number of roots (8.33), root fresh and dry weights (361.80 and 244.74 mg, respectively) were recorded in Kurdistan 5 genotype with 1,000 mg l-1 IBA. The maximum root length (5.84 cm) was observed in Kurdistan 5 genotype with 500 mg l-1 IBA that showed a significant difference compared to the control treatment (0.96 cm). The highest number of leaves per bud (7.33 at 500 mg l-1 IBA) and number of buds (5.00 at 1,000 mg l-1 IBA) were recorded in Kurdistan 1 genotype. The current study demonstrated that the different genotypes of R. damascena were in a difficult-toroot state, which suggests that cutting treatment with 1,000 mg l-1 IBA overcame the problem of the difficult-to-root state, and it can also enhance the rooting percentage in the studied genotypes.

Temporal variations in the rooting ability of cuttings of Populusbalsamifera and Salixplanifolia from natural clones–populations of subarctic Quebec

1993 ◽  
Vol 23 (12) ◽  
pp. 2603-2608 ◽  
Author(s):  
Gilles Houle ◽  
Patrice Babeux
Keyword(s):  
Root Length ◽  
Adventitious Roots ◽  
Growing Season ◽  
Temporal Variations ◽  
Bud Break ◽  
Root Number ◽  
Rooting Ability ◽  
Coefficients Of Variation ◽  
Site Restoration ◽  
Great Root

Temporal variations in the rooting ability of cuttings from five clones of Populusbalsamifera L. and five populations of Salixplanifolia Pursh from the Great Whale River valley in subarctic Quebec were determined. Cuttings were sampled monthly from May through October and rooted in a greenhouse. Root number and length of the longest root per cutting were determined 35 days after planting. There were significant variations among the six sampling dates in the rooting potential of cuttings of both species. A higher percentage of cuttings formed adventitious roots in May and June before or shortly after bud break than later, during the growing season; root number and length followed a similar trend. There was an increase in the rooting ability of cuttings of both species towards the fall period. Salixplanifolia produced more roots per cutting than P. balsamifera early in the season (i.e., May and June), but later in the season the differences between the two species were not great; root length showed no such trend. Differences among clones–populations in the rooting potential of cuttings were large for both species. Coefficients of variation for root number were lower in spring than later, during the growing season, for both P. balsamifera and S. planifolia. Seasonal trends in coefficients of variation for root length were not as evident as for root number. These results have significant implications for site restoration in the Subarctic. To optimize the rooting ability of cuttings and minimize the differences among clones–populations in rooting potential, cuttings should be sampled early in the season before bud break or shortly thereafter.

scholarly journals Determining the Rooting Ability of Hardwood Cuttings of Blueberry (Vaccinium corymbosum L.) cv. ‘Bluecrop’ Stored in Refrigerating Conditions

2020 ◽  
Vol 8 (3) ◽  
pp. 81
Author(s):  
Sabri Braha ◽  
Petrit Rama ◽  
Agim Zajmi
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Roots ◽  
Vaccinium Corymbosum ◽  
Mother Plant ◽  
Winter Period ◽  
Naphthalene Acetic Acid ◽  
Intact Control ◽  
Rooting Ability ◽  
Time Periods

This experiment has been carried out in order to determine the rooting ability of hardwood cuttings of the blueberry cultivar ‘Bluecrop’ collected during the deep dormant winter period in the last part of January. In order to induce the adventitious roots formation, the cuttings were stimulated using Indol Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) in various concentrations (1500, 3000, 4500 ppm), whilst one row in each box remained intact (control).  The collection of cuttings off the mother plant at the beginning of January enable the nutrients and other matter that help the process of rooting not to move from the base of cutting towards the top and consequently they remain at the base of cutting which results with quite a high rooting percentage (up to 72.5%) compared to the cuttings collected in February with rooting percentage of 67.5% and in March (52%). In both types of substrate, turf and turf-perlite 2:1 and in different time periods when cuttings have been collected, IBA proved to be better in inducing adventitious roots compared to NAA.

scholarly journals   Alanine conjugate of indole-3-butyric acid improves rooting of highbush blueberries

2012 ◽  
Vol 58 (No. 5) ◽  
pp. 236-241 ◽  
Author(s):  
S. Mihaljević ◽  
B. Salopek-Sondi
Keyword(s):  
Butyric Acid ◽  
Vaccinium Corymbosum ◽  
Highbush Blueberry ◽  
Stem Cuttings ◽  
Practical Application ◽  
Rooting Of Cuttings ◽  
Highbush Blueberries ◽  
Set Up ◽  
Indole 3 Acetic Acid

Auxins and their synthetic analogues are commonly used for rooting of cuttings, but their efficiency depends on experimental set-up and, even more importantly, on species or cultivar, and type of explants investigated. In attempt to improve rooting procedure for highbush blueberries (Vaccinium corymbosum L.), we investigated alanine conjugate of indole-3-butyric acid (IBA-Ala) as potential root-promoting compound and compared with commonly used auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The effect of different concentrations of auxins on the rooting of highbush blueberry stem cuttings (var. Bluecrop, Bluetta, Burlington and Jersey) and in vitro-derived microcuttings (var. Jersey) was investigated. Auxin treatments significantly promoted rooting of all four varieties in comparison to control. The most efficient rooting promoter in all varieties appeared IBA-Ala (provided up to 83%, and 93% of the rooted cuttings and microcuttings, respectively). Furthermore, IBA-Ala caused a vigorous, well-branched root system that resulted in better acclimatization and survival of plants. Results suggest the application of IBA-Ala may be beneficial for rooting of difficult-to-root varieties of blueberry and related species and their practical application in the nursery industry.  

scholarly journals Genome-wide association study of rice rooting ability at the seedling stage

2020 ◽  
Author(s):  
Xin Xu ◽  
Junhua Ye ◽  
Yingying Yang ◽  
Mengchen Zhang ◽  
Qun Xu ◽  
...  
Keyword(s):  
Association Study ◽  
Root Length ◽  
Genetic Basis ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Root Number ◽  
Expression Levels ◽  
Rooting Ability ◽  
Genome Wide ◽  
Population Structure Analysis

Abstract BackgroundRice rooting ability is a complex agronomical trait that displays heterosis and plays an important role in rice growth and production. Only a few quantitative trait loci (QTLs) have been identified by bi-parental population. More genes or QTLs are required to dissect the genetic architecture of rice rooting ability.ResultsTo characterize the genetic basis for rice rooting ability, we used a natural rice population, genotyped by a 90K single nucleotide polymorphism (SNP) array, to identify the loci associated with rooting-related traits through the genome-wide association study (GWAS). Population structure analysis divided the natural population into two subgroups: indica and japonica. We measured four traits for evaluating rice rooting ability, namely root growth ability (RGA), maximum root length (MRL), root length (RL), and root number (RN). Using the association study in three panels consisting of one for the full population, one for indica, and one for japonica, 24 SNPs associated with rooting ability-related traits were identified. Through comparison of the relative expression levels and DNA sequences between germplasm with extreme phenotypes, results showed that LOC_Os05g11810 had non-synonymous variations at the coding region, which may cause differences in root number, and that the expression levels of LOC_Os04g09900 and LOC_Os04g10060 are closely associated with root length variation.ConclusionsThrough evaluation of the rice rooting ability-related traits and the association mapping, we provided useful information for understanding the genetic basis of rice rooting ability and also identified some candidate genes and molecular markers for rice root breeding.

scholarly journals Three Mussaenda Cultivars Propagated by Stem Cuttings Exhibit Variation in Rooting in Response to Hormone and Rooting Conditions

2000 ◽  
Vol 10 (4) ◽  
pp. 780-784
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Clonal Propagation ◽  
Stem Cuttings ◽  
Root Number ◽  
Root Numbers ◽  
Rooting Medium ◽  
Rooting Of Cuttings

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

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