scholarly journals Pea Cultivar Susceptibility and Inheritance of Resistance to the Lentil Strain (Pathotype P2) of Pea Seedborne Mosaic Virus

1997 ◽  
Vol 122 (3) ◽  
pp. 325-328 ◽  
Author(s):  
K. Kasimor ◽  
J.R. Baggett ◽  
R.O. Hampton
Keyword(s):  
Mosaic Virus ◽  
Relative Proportion ◽  
Recessive Gene ◽  
Plant Introduction ◽  
Yellow Mosaic Virus ◽  
Breeding Lines ◽  
Commercial Cultivars ◽  
Segregation Ratios ◽  
Linkage Of Genes ◽  
Cultivar Susceptibility

Commercial pea (Pisum sativum L.) cultivars, plant introduction (PI) lines, and Oregon State Univ. (OSU) breeding lines were tested for resistance to pathotype P2 (lentil strain) and pathotype P1 (type strain) of pea seedborne mosaic virus (PSbMV) and to bean yellow mosaic virus (BYMV) to assess the relative proportion of resistant and susceptible pea genotypes. Of the 161 commercial cultivars tested, 117 (73%) were resistant and 44 were susceptible to PSbMV-P2. Of these PSbMV-P2-resistant cultivars, 115 were tested for resistance to BYMV and all were resistant. Of the 44 PSbMV-P2-susceptible cultivars, 43 were tested for BYMV susceptibility and all were infected except two, `Quincy' and `Avon', both of which were susceptible to a BYMV isolate in another laboratory. Of 138 commercial cultivars inoculated with PSbMV-P1, all were susceptible. All PI lines and OSU breeding lines that were resistant to PSbMV-P1 were resistant also to PSbMV-P2. The high percentage of commercial cultivars resistant to PSbMV-P2 was probably attributable to the close linkage of genes sbm-2 and mo and the widespread use by breeders of BYMV-resistant `Perfection' and `Dark Skin Perfection' in developing new pea cultivars. Segregation ratios in progenies of three separate crosses between PSbMV-P2-resistant and PSbMV-P2-susceptible cultivars closely fit the expected 3 susceptible: 1 resistant ratio expected for resistance conferred by a single recessive gene.

scholarly journals Identification of Resistance to Potato yellow mosaic virus-Trinidad Isolate (PYMV-TT) Among Lycopersicon Species

Plant Disease ◽  
2003 ◽  
Vol 87 (6) ◽  
pp. 686-691 ◽  
Author(s):  
S. N. Rampersad ◽  
P. Umaharan
Keyword(s):  
Mosaic Virus ◽  
Germ Plasm ◽  
Blot Hybridization ◽  
Yellow Mosaic Virus ◽  
Dot Blot ◽  
Breeding Lines ◽  
Dot Blot Hybridization ◽  
Lycopersicon Species ◽  
Commercial Cultivars ◽  
Leaf Curl Virus

Three studies were carried out with the objective of identifying resistance to Potato yellow mosaic virus-Trinidad isolate (PYMV-TT) among Lycopersicon species through field screening and using field-inoculated infector rows. The presence of PYMV-TT was confirmed using dot blot hybridization assays. In the first study, eight commercial cultivars of tomato were tested for resistance. In a subsequent study, 11 breeding lines showing high levels of resistance to Tomato mottle virus (ToMoV-Florida) and six lines resistant to Tomato yellow leaf curl virus (TYLCV-Old World Begomovirus) were screened for resistance to PYMV-TT. All breeding lines and the commercial cultivars tested were susceptible to PYMV-TT infection. The third study involved screening 92 accessions from a representative core collection of wild Lycopersicon germ plasm. PYMV-TT was not detected in individuals of 22 accessions.

scholarly journals EVALUATION OF WATERMELON AND RELATED GERMPLASM FOR RESISTANCE TO ZUCCHINI YELLOW MOSAIC VIRUS

HortScience ◽  
1992 ◽  
Vol 27 (11) ◽  
pp. 1173a-1173 ◽  
Author(s):  
George Boyhan ◽  
Joseph D. Norton ◽  
Barry J. Jacobsen ◽  
Bruce R. Abrahams
Keyword(s):  
Mosaic Virus ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Citrullus Colocynthis ◽  
Breeding Lines ◽  
First Report ◽  
Plant Introductions ◽  
Commercial Cultivars

Of 153 plant introductions (PI), breeding lines, and commercial cultivars tested by mechanical inoculations in the greenhouse, only PI 482261-1, `Egun', PI 494528, PI 386026, and PI 386025 showed any resistance to the Florida strain of zucchini yellow mosaic virus. PI 482261-1 and `Egun' are Citrullus lanatus, the others are citron types, Citrullus colocynthis. This is the first report of resistance in PI 386026, PI 386025, and the cultivar `Egun'.

scholarly journals Detecting bean golden yellow mosaic virus in bean breeding lines and in the common legume weed Macroptilium lathyroides in Puerto Rico.

1969 ◽  
Vol 85 (3-4) ◽  
pp. 165-176
Author(s):  
Lydia I. Rivera-Vargas ◽  
Vilmaris Bracero-Acosta ◽  
James S. Beaver ◽  
Dan E. Purcifull ◽  
Jane E. Polston ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Phaseolus Vulgaris L ◽  
Breeding Lines ◽  
Golden Yellow ◽  
Enzymelinked Immunosorbent Assay ◽  
The Common ◽  
Polymerase Chain ◽  
Macroptilium Lathyroides

Bean golden yellow mosaic virus (BGYMV) is a geminivirus transmitted by whiteflies (Genus: Bemisia). This virus causes significant fosses in common bean (Phaseolus vulgaris L.). Serological techniques such as enzymelinked immunosorbent assay (ELISA) have been widely used for detection of viruses. We evaluated existing monoclonal antibodies (3F7,2G5 and 5C5) for the detection of BGYMV isolates in bean fines in Puerto Rico. Monoclonal antibody 3F7 was the most effective in detecting the virus in tissues of line DOR 364 and susceptible cuftivars Top Crop and Quest. However, it was not effective in the detection of BGYMV in lines of DOR 303, which showed typical symptoms. Sampfes from Macroptilium lathyroides, a weed that might be a possible reservoir of the virus, were also tested for viraf infection. ELISA tests were inconclusive for detection of geminiviruses in M. lathyroides. Polymerase Chain Reaction (PCR) was also used to complement BGYMV diagnosis in M. lathyroides and in bean lines that showed symptoms but were negative for the ELfSA test. Two sets of primers, specific for Begomovirus such as BGYMV, were used in PCR experiments. Using PCR, we were able to detect the virus in the line DOR 303 and in M. lathyroides tissues.

scholarly journals Development and Field Evaluation of Multiple Virus-Resistant Bottle Gourd (Lagenaria siceraria)

Plant Disease ◽  
2013 ◽  
Vol 97 (8) ◽  
pp. 1057-1062 ◽  
Author(s):  
K.-S. Ling ◽  
A. Levi ◽  
S. Adkins ◽  
C. S. Kousik ◽  
G. Miller ◽  
...  
Keyword(s):  
South Carolina ◽  
Mosaic Virus ◽  
Broad Spectrum ◽  
Virus Resistance ◽  
Zucchini Yellow Mosaic Virus ◽  
The United States ◽  
Bottle Gourd ◽  
Yellow Mosaic Virus ◽  
Lagenaria Siceraria ◽  
Breeding Lines

In an effort to develop bottle gourd (Lagenaria siceraria) as a widely adapted rootstock for watermelon grafting, we sought to identify lines with broad resistance to several cucurbit viruses that are economically important in the United States. Preliminary analysis under greenhouse conditions indicated that the currently available commercial watermelon rootstocks were either highly susceptible or somewhat tolerant to one or more viruses. However, in greenhouse screening, several breeding lines of bottle gourd displayed broad-spectrum resistance to four viruses tested, including Zucchini yellow mosaic virus, Watermelon mosaic virus (WMV), Papaya ringspot virus watermelon strain (PRSV-W), and Squash vein yellowing virus. Resistance to PRSV-W and WMV was confirmed through field trials in two consecutive years at two different locations in South Carolina. Two breeding lines (USVL#1-8 and USVL#5-5) with broad-spectrum virus resistance could be useful materials for watermelon rootstock development.

scholarly journals Yield and Virus Resistance of Summer Squash Cultivars and Breeding Lines in North Carolina

1998 ◽  
Vol 8 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Jonathan R. Schultheis ◽  
S. Alan Walters
Keyword(s):  
North Carolina ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Breeding Lines ◽  
Summer Squash

Yellow and zucchini squash (Cucurbita pepo L.) cultigens (breeding lines and cultivars) were evaluated over a 2-year (1995 and 1996) period in North Carolina. Yellow squash cultigens that performed well (based on total marketable yields) were `Destiny III', `Freedom III', `Multipik', XPHT 1815, and `Liberator III' in Fall 1995 and HMX 4716, `Superpik', PSX 391, `Monet', `Dixie', XPH 1780, and `Picasso' in Spring 1996. Some of the yellow squash cultigens evaluated had superior viral resistance: XPHT 1815, XPHT 1817, `Freedom III', `Destiny III', `Freedom II', TW 941121, `Prelude II', and `Liberator III' in Fall 1995 and XPHT 1815, `Liberator III', `Prelude II', and `Destiny III' in Fall 1996; all these cultigens were transgenic. The yellow squash cultigens that performed well (based on total marketable yields) in the Fall 1995 test had transgenic virus resistance (`Destiny III', `Freedom III', XPHT 1815, and `Liberator III') or had the Py gene present in its genetic background (`Multipik'). Based on total marketable yields, the best zucchini cultigens were XPHT 1800, `Tigress', XPHT 1814, `Dividend' (ZS 19), `Elite', and `Noblesse' in Fall 1995; and `Leonardo', `Tigress', `Hurricane', `Elite', and `Noblesse' in Spring 1996. The zucchini cultigens with virus resistance were TW 940966, XPHT 1814, and XPHT 1800 in Fall 1995 and XPHT 1800, XPHT 1776, XPHT 1777, XPHT 1814, and XPHT 1784 in Fall 1996. Even though TW 940966 had a high level of resistance in the Fall 1995 test, it was not as high yielding as some of the more susceptible lines. Viruses detected in the field were papaya ringspot virus (PRSV) and watermelon mosaic virus (WMV) for Fall 1995; while PRSV, zucchini yellow mosaic virus (ZYMV), and WMV were detected for Fall 1996. Summer squash cultigens transgenic for WMV and ZYMV have potential to improve yield, especially during the fall when viruses are more prevalent. Most transgenic cultigens do not possess resistance to PRSV, except XPHT 1815 and XPHT 1817. Papaya ringspot virus was present in the squash tests during the fall of both years. Thus, PRSV resistance must be transferred to the transgenic cultigens before summer squash can be grown during the fall season without the risk of yield loss due to viruses.

scholarly journals Different Haplotypes Encode the Same Protein for Independent Sources of Zucchini Yellow Mosaic Virus Resistance in Cucumber

HortScience ◽  
2017 ◽  
Vol 52 (8) ◽  
pp. 1040-1042 ◽  
Author(s):  
Axel O. Ramírez-Madera ◽  
Michael J. Havey
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Recessive Gene ◽  
Genomic Region ◽  
Yellow Mosaic Virus ◽  
Single Recessive Gene ◽  
Coding Region ◽  
Coding Regions ◽  
Genetic Uniformity ◽  
Vacuolar Protein

Cucumber (Cucumis sativus) production is negatively affected by Zucchini yellow mosaic virus (ZYMV). Three sources of ZYMV resistance have been commercially deployed and all three resistances are conditioned by a single recessive gene. A vacuolar protein sorting–associated protein 4-like (VPS4-like) gene has been proposed as a candidate for ZYMV resistance from cucumber line A192-18. We analyzed the genomic region across the VPS4-like gene for three independent sources of ZYMV resistance in cucumber (A192-18, Dina-1, and TMG-1) and identified three haplotypes across the coding region and considerable variation in the introns. However, the haplotypes in the coding regions of the VPS4-like gene of A192-18, Dina-1, and TMG-1 encode the same protein sequence, revealing the genetic uniformity for ZYMV resistance from diverse germplasm sources.

scholarly journals Activation of the Salicylic Acid Signaling Pathway Enhances Clover yellow vein virus Virulence in Susceptible Pea Cultivars

2009 ◽  
Vol 22 (2) ◽  
pp. 166-175 ◽  
Author(s):  
Go Atsumi ◽  
Uiko Kagaya ◽  
Hiroaki Kitazawa ◽  
Kenji Suto Nakahara ◽  
Ichiro Uyeda
Keyword(s):  
Cell Death ◽  
Salicylic Acid ◽  
Mosaic Virus ◽  
Signaling Pathway ◽  
Dominant Gene ◽  
Plant Introduction ◽  
Yellow Vein ◽  
Yellow Mosaic Virus ◽  
Clover Yellow Vein Virus ◽  
Sa Signaling

The wild-type strain (Cl-WT) of Clover yellow vein virus (ClYVV) systemically induces cell death in pea cv. Plant introduction (PI) 118501 but not in PI 226564. A single incompletely dominant gene, Cyn1, controls systemic cell death in PI 118501. Here, we show that activation of the salicylic acid (SA) signaling pathway enhances ClYVV virulence in susceptible pea cultivars. The kinetics of virus accumulation was not significantly different between PI 118501 (Cyn1) and PI 226564 (cyn1); however, the SA-responsive chitinase gene (SA-CHI) and the hypersensitive response (HR)-related gene homologous to tobacco HSR203J were induced only in PI 118501 (Cyn1). Two mutant viruses with mutations in P1/HCPro, which is an RNA-silencing suppressor, reduced the ability to induce cell death and SA-CHI expression. The application of SA and of its analog benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH) partially complemented the reduced virulence of mutant viruses. These results suggest that high activation of the SA signaling pathway is required for ClYVV virulence. Interestingly, BTH could enhance Cl-WT symptoms in PI 226564 (cyn1). However, it could not enhance symptoms induced by White clover mosaic virus and Bean yellow mosaic virus. Our report suggests that the SA signaling pathway has opposing functions in compatible interactions, depending on the virus–host combination.

scholarly journals Two Genes from Phaseolus coccineus Confer Resistance to Bean Golden Yellow Mosaic Virus in Common Bean

2007 ◽  
Vol 132 (4) ◽  
pp. 530-533 ◽  
Author(s):  
Juan M. Osorno ◽  
Carlos G. Muñoz ◽  
James S. Beaver ◽  
Feiko H. Ferwerda ◽  
Mark J. Bassett ◽  
...  
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Interspecific Cross ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Phaseolus Coccineus ◽  
Yellow Mosaic Virus ◽  
Sources Of Resistance ◽  
Sequence Characterized Amplified Region ◽  
Golden Yellow

Bean golden yellow mosaic virus (BGYMV), incited by a whitefly (Bemisia tabaci Gennadius) transmitted geminivirus, is an important disease that can limit common bean (Phaseolus vulgaris L.) production in Central America, the Caribbean, and southern Florida. Only a few genes are currently deployed in BGYMV-resistant common bean cultivars. The identification of novel sources of resistance would help bean breeders broaden the genetic base of resistance to this important virus. Phaseolus coccineus L. germplasm accession G35172 was found by International Center for Tropical Agriculture scientists to be resistant to BGYMV. Populations derived from an interspecific cross between P. vulgaris and P. coccineus were evaluated to study the inheritance of resistance to BGYMV. Segregation ratios of F2 plants and other populations suggest that BGYMV resistance from P. coccineus is controlled by two genes. A recessive gene, with the proposed symbol bgm-3, confers resistance to leaf chlorosis and a dominant gene, with the proposed name Bgp-2, prevents pod deformation in the presence of BGYMV. Results from allelism tests with previously reported BGYMV resistance genes (bgm, bgm-2, and Bgp) and the absence of the SR-2 sequence-characterized amplified region marker for bgm support the hypothesis that bgm-3 and Bgp-2 are different genes for BGYMV resistance.

scholarly journals (11) Inheritance of Resistance to Zucchini Yellow Mosaic Virus-Florida Strain in Watermelon

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1039E-1039
Author(s):  
Nihat Guner ◽  
Todd C. Wehner
Keyword(s):  
Mosaic Virus ◽  
New Hampshire ◽  
Zucchini Yellow Mosaic Virus ◽  
Recessive Gene ◽  
Yellow Mosaic Virus ◽  
Inheritance Of Resistance ◽  
Single Recessive Gene ◽  
Leaf Stage ◽  
True Leaf ◽  
Severe Isolate

Inheritance of resistance to zucchini yellow mosaic virus-Florida strain (ZYMV-FL) was studied in the resistant watermelon accession of PI 595203 (Citrulluslanatus var. lanatus), an egusi type originally collected in Nigeria. The F1, F2, and BC1 generations derived from the cross `Calhoun Gray' × PI 595203 and `New Hampshire Midget' × PI 595203 were used to study the inheritance of resistance to ZYMV-FL. Seedlings were inoculated with a severe isolate of ZYMV-FL at the first true leaf stage and rated on a 0 to 9 scale, based on the severity of virus symptoms. A single recessive gene was found to control resistance, for which we propose the symbol zym-FL2. The gene probably was not allelic to the previously published gene, zym-FL, for resistance to the Florida strain of ZYMV in the accession PI 482261, since PI 482261 was not resistant to the ZYMV-FL isolate used in our tests.

Development of Mapping Population and Validation of Molecular Markers Associated with MYMV Resistance in Mungbean

2020 ◽  
Author(s):  
S. C. Mogali ◽  
B. N. Abhilash ◽  
L. G. Jaggal
Keyword(s):  
Molecular Markers ◽  
Mosaic Virus ◽  
Mapping Population ◽  
Virus Disease ◽  
Recessive Gene ◽  
Yellow Mosaic Virus ◽  
Research Station ◽  
Phenotypic Variance ◽  
Marker Analysis ◽  
Mungbean Yellow Mosaic Virus

Background: Mungbean yellow mosaic virus disease is the most devastating disease on Mungbean production. The virus is transmitted by whitefly and can cause yield losses from 75 to 100 per cent. The development of mungbean cultivars resistant to both virus and its vector is considered as one of the most desirable means of managing the disease as it is environmentally safe and highly efficient. The selection of resistant genotypes in conventional methods is complex and time consuming. Hence, the use of molecular markers linked with resistance genes is powerful as it hastens the breeding programmes. The current study was aimed to develop mapping population and to validate molecular markers associated with Mungbean yellow mosaic virus (MYMV). Methods: The present investigation was carried out with 260 F2 individuals that were derived from crossing DGGV-2 and IPM 2-14 during Kharif-2017 at Main Agril Research Station, UAS, Dharwad. Hybrid seeds of this cross were harvested individually and sown during rabi 2017 along with the two parents, as checks for distinguishing the true hybrids. Hybridity of F1s was confirmed through molecular marker analysis and the true F1s were selfed to raise the F2 generation. Result: Of the 24 previously reported simple sequence repeat markers used for detecting the polymorphism, two markers viz., CEDG305 and CEDG115 were found to be polymorphic between DGGV-2 and IPM-2-14. Two hundred and sixty F2 plants segregated in the ratio of 3 S:1 R (202 susceptible: 58 resistant) as phenotypic and 1: 2 :1 as genotypic ratio implying that single recessive gene controlled resistance. Single marker analysis revealed that the molecular markers CEDG305 and CEDG115 were associated with MYMV resistance with a phenotypic variance of 24.5 and 10.3 per cent respectively.

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