Generation of a Mutant Population for TILLING Common Bean Genotype BAT 93

Common bean (Phaseolus vulgaris) is the major food legume worldwide, making it an important target for novel approaches of genetic analysis. This study evaluated the use of ethyl methane sulfonate (EMS) for the generation of a mutant population for targeted induced local lesions in genomes (TILLING) in common bean. TILLING is a powerful reverse genetics approach that uses a large mutant population for identification of mutants in loci of interest. Based on overall survival, development, and yield of treated seed, 40 mm EMS was found to be an appropriate concentration for the generation of a mutant population in common bean genotype BAT 93. Higher concentrations of EMS resulted in survival rates of less than 10% and lower concentrations resulted in the generation of fewer mutants. Based on TILLING results from other species, a population of 5000 lines is estimated to be sufficient for saturation of the common bean genome. Phenotypic mutation frequencies and the isolation of targeted mutations in the BAT 93 mutant population indicate that mutagenesis was effective.

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Asymmetrical leaves induced by water deficit show asymmetric photosynthesis in common bean

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202005000200005 ◽

2005 ◽

Vol 17 (2) ◽

pp. 223-227 ◽

Cited By ~ 7

Author(s):

Gustavo Maia Souza ◽

Juliana de Oliveira Fernandes Viana ◽

Ricardo Ferraz de Oliveira

Keyword(s):

Common Bean ◽

Water Deficit ◽

Net Photosynthesis ◽

Developmental Instability ◽

Gas Exchanges ◽

Symmetric Plane ◽

Bean Genotype ◽

Common Bean Genotype ◽

Asymmetric Growth ◽

Effects Of Drought

In this study we tested the hypothesis that leaf asymmetric growth induced by water deficit in common bean (Phaseolus vulgaris L.) might be correlated with different net photosynthesis rates between the two opposed leaflets (right and left) considering a bilateral symmetric plane. In order to induce developmental instability, the drought-sensitive common bean genotype "Jalo Precoce" was subjected to periods of suspended irrigation during its vegetative growth. Developmental instability was evaluated by measurements of leaflets asymmetry, and the effects of drought on gas exchanges were taken in both symmetrical leaflets (right and left) in relation to the central leaflet. Water deficit induced an increase of 80 % in leaf asymmetry. Net photosynthesis of P. vulgaris was affected by water deficit in two ways, reducing its physiological yield and increasing its variability between leaflets. Thus, as we hypothesized, the increase in developmental instability, measured by leaf symmetry deviations, was supported by a variable net photosynthesis distribution in the leaves induced by drought.

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FARMER PARTICIPATION IN COMMON BEAN GENOTYPE EVALUATION: THE CASE OF EASTERN ETHIOPIA

Experimental Agriculture ◽

10.1017/s0014479797004079 ◽

1997 ◽

Vol 33 (4) ◽

pp. 399-408 ◽

Cited By ~ 15

Author(s):

F. MEKBIB

Keyword(s):

Common Bean ◽

Selection Criteria ◽

Navy Bean ◽

Eastern Ethiopia ◽

Bean Genotype ◽

Common Bean Genotype ◽

Genotype Evaluation ◽

On Farm ◽

Yield Trials ◽

Selection Of

Advanced genotypes of common bean (Phaseolus vulgaris L.) were tested on-station and on-farm to evaluate their performance and acceptability under farmers' circumstances. Farmers were given seed of the genotypes to plant a minimum area of 100 m2 to be grown as a sole crop or intercropped with sorghum according to their preference. Yield, seed colour, taste, maturity time, disease reaction, growth habit and many other bean characteristics were assessed by farmers. The promising genotypes were higher yielding than the farmers' genotypes in most testing sites. Pairwise ranking of the genotypes showed that in export-oriented western Hararghe, white pea bean (navy bean) type Awash-1 and food bean G-2816 were preferred. In eastern Hararghe GLP ×92 and G-2816 were highly preferred but white pea beans were not preferred. Genotypes in advanced observation nurseries were also evaluated by the farmers and some of the genotypes have been promoted for further testing in yield trials. Selection criteria have been proposed by the farmers in each region. Future breeding and selection of genotypes will incorporate the proposed selection criteria and priorities of the two regions.

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Distinction between a transgenic and a conventional common bean genotype by 1H HR-MAS NMR

Food Chemistry ◽

10.1016/j.foodchem.2013.05.123 ◽

2013 ◽

Vol 141 (3) ◽

pp. 2841-2847 ◽

Cited By ~ 25

Author(s):

Rafael Choze ◽

Glaucia B. Alcantara ◽

Elenilson de G. Alves Filho ◽

Lorena Mara A. e Silva ◽

Josias C. Faria ◽

...

Keyword(s):

Common Bean ◽

Mas Nmr ◽

Bean Genotype ◽

Common Bean Genotype

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Quality of Protein From the Common Bean

Case Medical Research ◽

10.31525/ct1-nct04118517 ◽

2019 ◽

Author(s):

Keyword(s):

Common Bean ◽

The Common

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Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

Scientific Reports ◽

10.1038/s41598-021-88051-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Evdoxia Efstathiadou ◽

Georgia Ntatsi ◽

Dimitrios Savvas ◽

Anastasia P. Tampakaki

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Phylogenetic Affiliation ◽

Rhizobial Inoculation ◽

Rhizobial Species ◽

The Common ◽

First Time ◽

Of Greece ◽

Rhizobial Isolates

AbstractPhaseolus vulgaris (L.), commonly known as bean or common bean, is considered a promiscuous legume host since it forms nodules with diverse rhizobial species and symbiovars. Most of the common bean nodulating rhizobia are mainly affiliated to the genus Rhizobium, though strains belonging to Ensifer, Pararhizobium, Mesorhizobium, Bradyrhizobium, and Burkholderia have also been reported. This is the first report on the characterization of bean-nodulating rhizobia at the species and symbiovar level in Greece. The goals of this research were to isolate and characterize rhizobia nodulating local common bean genotypes grown in five different edaphoclimatic regions of Greece with no rhizobial inoculation history. The genetic diversity of the rhizobial isolates was assessed by BOX-PCR and the phylogenetic affiliation was assessed by multilocus sequence analysis (MLSA) of housekeeping and symbiosis-related genes. A total of fifty fast-growing rhizobial strains were isolated and representative isolates with distinct BOX-PCR fingerpriniting patterns were subjected to phylogenetic analysis. The strains were closely related to R. anhuiense, R. azibense, R. hidalgonense, R. sophoriradicis, and to a putative new genospecies which is provisionally named as Rhizobium sp. I. Most strains belonged to symbiovar phaseoli carrying the α-, γ-a and γ-b alleles of nodC gene, while some of them belonged to symbiovar gallicum. To the best of our knowledge, it is the first time that strains assigned to R. sophoriradicis and harbored the γ-b allele were found in European soils. All strains were able to re-nodulate their original host, indicating that they are true microsymbionts of common bean.

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Inheritance and development of molecular markers linked to angular leaf spot resistance genes in the common bean accession G10909

Molecular Breeding ◽

10.1007/s11032-010-9461-x ◽

2010 ◽

Vol 28 (1) ◽

pp. 57-71 ◽

Cited By ~ 17

Author(s):

George S. Mahuku ◽

María Antonia Henríquez ◽

Carmenza Montoya ◽

Carlos Jara ◽

Henry Teran ◽

...

Keyword(s):

Molecular Markers ◽

Common Bean ◽

Resistance Genes ◽

Leaf Spot ◽

Angular Leaf Spot ◽

The Common

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Genetic gain in the breeding program of common beans at IAC from 1989 to 2007

Cropp Breeding and Applied Biotechnology ◽

10.1590/s1984-70332010000400007 ◽

2010 ◽

Vol 10 (4) ◽

pp. 329-336 ◽

Cited By ~ 16

Author(s):

Alisson Fernando Chiorato ◽

Sérgio Augusto Morais Carbonell ◽

Roland Vencovsky ◽

Nelson da Silva Fonseca Júnior ◽

José Baldin Pinheiro

Keyword(s):

Grain Yield ◽

Common Bean ◽

Genetic Gain ◽

Grain Quality ◽

The State ◽

Breeding Program ◽

Common Beans ◽

The Common ◽

The Mean ◽

Second Period

The goal of the present work was to evaluate the genetic gain obtained in grain yield for the common bean genotypes from 1989 until 2007, at the Instituto Agronômico de Campinas, in the state of São Paulo. Genetic gain has been separated into two research periods; the first, from 1989 to 1996, and the second, from 1997 to 2007. In the first period, a genetic gain of 1.07 % per year was obtained, whereas for the second period, the gain was zero. However, the mean yield of the evaluated lines was approximately 1000 kg ha-1 superior to the figures obtained in the first period. The main cause for the absence of genetic gain in the second period is that the focus of the breeding program was changed to grain quality. The individualized analysis of the genotypes with carioca grains in the second period indicated the lack of genetic gain during the investigated period.

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