scholarly journals Genetic Mapping of PcDw Determining Pear Dwarf Trait

2011 ◽  
Vol 136 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Caihong Wang ◽  
Yike Tian ◽  
Emily J. Buck ◽  
Susan E. Gardiner ◽  
Hongyi Dai ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Rapd Markers ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Pyrus Pyrifolia ◽  
Chi Square ◽  
Sequence Characterized Amplified Regions ◽  
Chi Square Analysis ◽  
Simple Sequence

European pear (Pyrus communis) ‘Aihuali’ carrying the dwarf character originating from ‘Nain Vert’ was crossed with ‘Chili’ (Pyrus bretschneideri). A total of 352 F1 progenies was produced to investigate the inheritance of the dwarf trait, and 111 of these were used to develop molecular markers. Chi-square analysis showed that the character fitted a 1:1 ratio indicative of a single dominant gene, which we have named PcDw. Using a bulked segregant analysis approach with 500 random amplified polymorphic DNA (RAPD) and 51 simple sequence repeat (SSR) markers from pear (Pyrus pyrifolia and P. communis) and apple (Malus ×domestica), four markers were identified as cosegregating with the dwarf character. Two of these were fragments produced by the S1212 and S1172 RAPD primers, and the other two were the pear SSR markers KA14 and TsuENH022. The RAPD markers were converted into sequence-characterized amplified regions (SCARs) and designated S1212-SCAR318 and S1172-SCAR930 and, with the SSR markers KA14 and TsuENH022, were positioned 5.9, 9.5, 8.2, and 0.9 cM from the PcDw gene, respectively. Mapping of the KA14 and TsuENH022 markers enabled the location of the PcDw gene on LG 16 of the pear genetic linkage map.

scholarly journals Identification and Characterization of Random Amplified Polymorphic DNA Markers Linked to a Major Gene (Cr2) for Resistance to Cronartium ribicola in Pinus monticola

2006 ◽  
Vol 96 (4) ◽  
pp. 395-399 ◽  
Author(s):  
Jun-Jun Liu ◽  
Abul K. M. Ekramoddoullah ◽  
Rich S. Hunt ◽  
Arezoo Zamani
Keyword(s):  
Dna Markers ◽  
Positional Cloning ◽  
Rapd Markers ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Cronartium Ribicola ◽  
R Genes ◽  
Pinus Monticola ◽  
White Pine

DNA markers tightly linked to resistance (R) genes provide a very powerful tool for both marker-assisted selection in plant breeding and positional cloning of R genes. In the present study, a linkage of random amplified polymorphic DNA (RAPD) markers to the single dominant gene (Cr2) for resistance to white pine blister rust fungus (Cronartium ribicola) was investigated in western white pine (Pinus monticola). A mapping population of 128 individual megagametophytes was generated from seeds of a heterozygous resistant tree (Cr2/cr2), and the corresponding seedlings of each megagametophyte were subjected to the test of phenotype segregation by inoculation with C. ribicola. Bulked segregant analysis and haploid segregation analysis identified eight robust RAPD markers linked to Cr2. This constitutes the first Cr2 genetic linkage map spanning 84.7 cM with four markers only 3.2 cM from Cr2. One sequence (U256-1385) of these linked markers was significantly similar to the Ty3/gypsy-like long terminal direct repeats retrotransposons. Another marker, U570-843, had no significant similarity to any entry in either GenBank or the loblolly genomics data bank. As presumed that the average physical distance per centimorgan is about 10 Mb in P. monticola, it is probably unrealistic to use these DNA markers for positional cloning of the Cr2 gene.

scholarly journals Genetic diversity within and among two-spotted spider mite resistant and susceptible common bean genotypes

2017 ◽  
Vol 109 (3) ◽  
pp. 517
Author(s):  
Zeinab YOUSEFI ◽  
Zahra TAHMASEBI ◽  
Mohammad Javad Erfani MOGHADAM ◽  
Ali ARMINIAN
Keyword(s):  
Genetic Diversity ◽  
Common Bean ◽  
Ssr Markers ◽  
Rapd Markers ◽  
Spider Mite ◽  
Random Amplified Polymorphic Dna ◽  
Breeding Programs ◽  
Ssr Analysis ◽  
Two Spotted Spider Mite ◽  
Simple Sequence

<p>Two-spotted spider mite (<em>Tetranychus urticae </em><a title="Carl Ludwig Koch" href="https://en.wikipedia.org/wiki/Carl_Ludwig_Koch">C. L. Koch</a>, 1836), is one of the most destructive herbivores of common bean. Very little is known about the diversity among resistant sources in this crop. The present study was conducted to characterize 22 resistant and susceptible common bean genotypes by 8 Simple Sequence Repeats (SSRs) and 8 Random Amplified Polymorphic DNA (RAPD) markers. These SSR and RAPD primers produced 100 % and 81.8 % polymorphic bands. Based on RAPD fingerprints and SSR profiles, pairwise genetic similarity ranged from 0.0 to 0.857 and from 0.125 to 1, respectively. The resistant and susceptible common bean accessions were grouped together in the dendrograms generated from RAPD and SSR clustering analyses. The results indicate that RAPD and SSR analysis could be successfully used for the estimation of genetic diversity among genotypes. SSR markers could group genotypes according to their resistibility and susceptibility to the spotted spider mite but RAPD could not. Therefore, the SSR markers can facilitate the development of resistant common bean cultivars through breeding programs against <em>T. urticae</em>.</p>

scholarly journals Origin of the Black Shank Resistance Gene, Ph, in Tobacco Cultivar Coker 371-Gold

Plant Disease ◽  
2002 ◽  
Vol 86 (10) ◽  
pp. 1080-1084 ◽  
Author(s):  
E. S. Johnson ◽  
M. F. Wolff ◽  
E. A. Wernsman ◽  
W. R. Atchley ◽  
H. D. Shew
Keyword(s):  
Nicotiana Tabacum ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Nicotiana Plumbaginifolia ◽  
Black Shank ◽  
Breeding Lines ◽  
Tobacco Cultivar ◽  
Qualitative Resistance ◽  
Interspecific Transfer

Flue-cured tobacco (Nicotiana tabacum) cultivar Coker 371-Gold (C 371-G) possesses a dominant gene, Ph, that confers high resistance to black shank disease, caused by race 0 of the soil-borne pathogen Phytophthora parasitica var. nicotianae. The origin of this gene is unknown. Breeding lines homozygous for the Ph gene were hybridized with NC 1071 and L8, flue-cured and burley genotypes known to possess qualitative resistance genes from Nicotiana plumbaginifolia and N. longiflora, respectively. The F1 hybrids were out-crossed to susceptible testers and the progenies evaluated in field black shank nurseries and in greenhouse disease tests with P. parasitica var. nicotianae race 0. Results showed that Ph was allelic to Php from N. plumbaginifolia in NC 1071. Testcross populations of hybrids between burley lines homozygous for Ph and L8, possessing Phl from N. longiflora, showed that Ph and Phl integrated into the same tobacco chromosome during interspecific transfer. Nevertheless, the two loci were estimated to be 3 cM apart. Random amplified polymorphic DNA (RAPD) analyses of the testcross progenies confirmed that recombination between the two loci was occurring. Forty-eight RAPD markers linked to Ph in doubled haploid lines were used in cluster analyses with multiple accessions of N. longiflora and N. plumbaginifolia, breeding lines L8, NC 1071, and DH92-2770-40, and cultivars K 326, Hicks, and C 371-G. A cladogram or region tree confirmed the data obtained from field and greenhouse trials, that Ph, transferred from C 371-G to DH92-2770-40, and Php in NC 1071 were allelic and originated from N. plumbaginifolia.

Identification of coupling and repulsion phase RAPD markers for powdery mildew resistance gene er-1 in pea

Genome ◽  
1998 ◽  
Vol 41 (3) ◽  
pp. 440-444 ◽  
Author(s):  
K R Tiwari ◽  
G A Penner ◽  
T D Warkentin
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Rapd Markers ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Powdery Mildew Resistance Gene ◽  
Erysiphe Pisi ◽  
Mildew Resistance ◽  
Repulsion Phase

Powdery mildew is a serious disease of pea caused by the obligate parasite Erysiphe pisi Syd. Random amplified polymorphic DNA (RAPD) analysis has emerged as a cost-effective and efficient marker system. The objective of this study was to identify RAPD markers for powdery mildew resistance gene er-1. The resistant cultivar Highlight (carrying er-1) and the susceptible cultivar Radley were crossed, and F3 plants were screened with Operon (OP) and University of British Columbia (UBC) primers, using bulked segregant analysis. A total of 416 primers were screened, of which amplicons of three Operon primers, OPO-18, OPE-16, and OPL-6, were found to be linked to er-1. OPO-181200 was linked in coupling (trans to er-1) and no recombinants were found. OPE-161600 (4 ± 2 cM) and OPL-61900 (2 ± 2 cM) were linked in repulsion (cis to er-1). The fragments OPO-181200 and OPE-161600 were sequenced and specific primers designed. The specific primer pair Sc-OPO-181200 will be useful in identifying homozygous resistant individuals in F2 and subsequent segregating generations. Sc-OPE-161600 will have greatest utility in selecting heterozygous BC\dn6 nF1 individuals in backcross breeding programs.Key words: bulked segregant analysis,Erysiphe pisi, pea, RAPD.

scholarly journals Linkage Mapping in a Watermelon Population Segregating for Fusarium Wilt Resistance

2001 ◽  
Vol 126 (3) ◽  
pp. 344-350 ◽  
Author(s):  
Leigh K. Hawkins ◽  
Fenny Dane ◽  
Thomas L. Kubisiak ◽  
Billy B. Rhodes ◽  
Robert L. Jarret
Keyword(s):  
Ssr Markers ◽  
Fusarium Wilt ◽  
Rapd Markers ◽  
Bulked Segregant Analysis ◽  
Citrullus Lanatus ◽  
Linkage Groups ◽  
Mendelian Segregation ◽  
Marker Loci ◽  
Resistance Trait ◽  
Effective Use

Isozyme, randomly amplified polymorphic DNA (RAPD), and simple sequence repeats (SSR) markers were used to generate a linkage map in an F2 and F3 watermelon [Citrullus lanatus (Thumb.) Matsum. & Nakai] population derived from a cross between the fusarium wilt (Fusarium oxysporum f. sp. niveum) susceptible `New Hampshire Midget' and resistant PI 296341-FR. A 112.9 cM RAPD-based map consisting of 26 markers spanning two linkage groups was generated with F2 data. With F3 data, a 139 cM RAPD-based map consisting of 13 markers covering five linkage groups was constructed. Isozyme and SSR markers were unlinked. About 40% to 48% of the RAPD markers were significantly skewed from expected Mendelian segregation ratios in both generations. Bulked segregant analysis and single-factor analysis of variance were employed to identify RAPD markers linked to fusarium wilt caused by races 1 and 2 of F. oxysporum f. sp. niveum. Current linkage estimates between the resistance trait and the marker loci were too large for effective use in a marker-assisted selection program.

scholarly journals 078 Mapping of the Citrullus Genome using Populations Segregating for Fusarium Wilt Resistance

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 454E-454
Author(s):  
Leigh K. Hawkins ◽  
Fenny Dane ◽  
Thomas L. Kubisiak ◽  
Billy Rhodes
Keyword(s):  
Fusarium Wilt ◽  
Citrullus Lanatus ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Wilt Resistance ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Race 2 ◽  
Serious Disease ◽  
Simple Sequence

Fusarium wilt, caused by the soilborne fungus Fusarium oxysporum f.sp. niveum (FON), is a serious disease of the watermelon (Citrullus lanatus). Three races of this pathogen (races 0, 1, and 2) have been identified based on differential pathogenicity assays. Most commercially available cultivars are resistant to races 0 and 1. Inheritance for resistance to these races is thought to be controlled by a single dominant gene. No cultivars are resistant to race 2 and resistance is thought to be a quantitative trait. F2 lines derived from a cross between the Fusarium-resistant Citrullus lanatus PI296341, and the Fusarium-susceptible watermelon cultivar `New Hampshire Midget' were used to generate a RAPD-based map of the Citrullus genome. F2:3 families were assayed in the greenhouse for resistance to races 1 and 2. Those families that were either highly resistant or highly susceptible were used in identifying markers linked to Fusarium wilt resistance. A preliminary map of the Citrullus genome based on random amplified polymorphic DNA (RAPD) markers has been expanded with the inclusion of simple sequence repeats (SSRs), amplified fragment length polymorphisms (AFLPs), and isozymes.

Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1050-1056 ◽  
Author(s):  
V Chagué ◽  
T Fahima ◽  
A Dahan ◽  
G L Sun ◽  
A B Korol ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Rapd Markers ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Stripe Rust Resistance Gene

Microsatellite and random amplified polymorphic DNA (RAPD) primers were used to identify molecular markers linked to the Yr15 gene which confer resistance to stripe rust (Puccina striiformis Westend) in wheat. By using near isogenic lines (NILs) for the Yr15 gene and a F2 mapping population derived from crosses of these lines and phenotyped for resistance, we identified one microsatellite marker (GWM33) and one RAPD marker (OPA19800) linked to Yr15. Then, bulked segregant analysis was used in addition to the NILs to identify RAPD markers linked to the target gene. Using this approach, two RAPD markers linked to Yr15 were identified, one in coupling (UBC199700) and one in repulsion phase (UBC2121200). After Mapmaker linkage analysis on the F2 population, the two closest markers were shown to be linked to Yr15 within a distance of about 12 cM. The recombination rates were recalculated using the maximum likelihood technique to take into account putative escaped individuals from the stripe rust resistance test and obtain unbiased distance estimates. As a result of this study, the stripe rust resistance gene Yr15 is surrounded by two flanking PCR markers, UBC199700 and GWM33, at about 5 cM from each side.Key words: wheat, Triticum dicoccoides, Yr15 stripe rust resistance gene, genetic mapping, microsatellite markers, RAPD markers.

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