Marker-Assisted Selection in Public Breeding Programs

Crop Science ◽  
2004 ◽  
Vol 44 (6) ◽  
pp. 1895-1898 ◽  
Author(s):  
Jorge Dubcovsky
Keyword(s):  
Marker Assisted Selection ◽  
Breeding Programs

scholarly journals Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Doubled Haploid ◽  
Marker Assisted Selection ◽  
Triticum Aestivum L ◽  
Physiological Model ◽  
Yield Potential ◽  
Breeding Programs ◽  
Per Se ◽  
Genomic Regions ◽  
Fertility Traits ◽  
Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

scholarly journals Using markers in gene introgression breeding programs.

Genetics ◽  
1992 ◽  
Vol 132 (4) ◽  
pp. 1199-1210 ◽  
Author(s):  
F Hospital ◽  
C Chevalet ◽  
P Mulsant
Keyword(s):  
Population Size ◽  
Marker Assisted Selection ◽  
Gene Introgression ◽  
Selection Intensity ◽  
Breeding Programs ◽  
Introgression Breeding ◽  
Size Number ◽  
Theoretical Predictions ◽  
Two Generations ◽  
High Selection

Abstract We investigate the use of markers to hasten the recovery of the recipient genome during an introgression breeding program. The effects of time and intensity of selection, population size, number and position of selected markers are studied for chromosomes either carrying or not carrying the introgressed gene. We show that marker assisted selection may lead to a gain in time of about two generations, an efficiency below previous theoretical predictions. Markers are most useful when their map position is known. In the early generations, it is shown that increasing the number of markers over three per non-carrier chromosome is not efficient, that the segment surrounding the introgressed gene is better controlled by rather distant markers unless high selection intensity can be applied, and that selection on this segment first can reduce the selection intensity available for selection on non-carrier chromosomes. These results are used to propose an optimal strategy for selection on the whole genome, making the most of available material and conditions (e.g., population size and fertility, genetic map).

scholarly journals Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers

2007 ◽  
pp. 100-104
Author(s):  
Stella Molnár ◽  
Zsuzsanna Galbács ◽  
Gábor Halász ◽  
Sarolta Hoffmann ◽  
Anikó Veres ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Resistance Breeding ◽  
Breeding Programs ◽  
Pcr Rflp ◽  
Muscadinia Rotundifolia ◽  
Selection Of ◽  
Grape Breeding

Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitisspecies were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types ofmolecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.

scholarly journals Marker-assisted breeding of onion (A. cepa L.) maintainer line resistant to Downy mildew

2021 ◽  
pp. 34-39
Author(s):  
Ya. T. Eidlin ◽  
G. F. Monakhos ◽  
S. G. Monakhos
Keyword(s):  
Downy Mildew ◽  
Inbred Line ◽  
Marker Assisted Selection ◽  
Dna Marker ◽  
F1 Hybrids ◽  
Marker Assisted Breeding ◽  
Maintainer Line ◽  
Breeding Programs ◽  
Maternal Line ◽  
Two Generations

Relevance. F1 hybrids breeding programs of onion are based on the use of nuclear cytoplasmic male sterility (NCMS). Breeding and seed production based on NCMS suggest a development of a female sterile maternal line, a maintainer line and a paternal component. The development of an isogenic pair of sterile line – maintainer line is one of the most labor-, time-consuming and intellectually-intensive stages of a breeding program, the implementation of which becomes more complicated with an increase in the number of traits / genes for which selection is carried out.Methods and results. This paper presents a genetic scheme for the development of a Downy mildewresistant (pathogen P. destructor) maintainer line of onion using marker-assisted selection (MAS). F2 populations were obtained from the cross of the female maintainer inbred line, Bn1- (13) (genotype – cytN msms pdpd) and male inbred line resistant to Downy mildew, No. 136 (genotype – cytT MsMs PdPd). DNA marker DMR1 were used for Downy mildew resistance gene Pd, jnurf13 – for maintainer gene ms and marker system 5`cob: orfA501 – for N, S and T cytoplasms. It was shown that the MAS application allow to develop a stable Downy mildew resistant maintainer line cytN msms PdPd in two generations.

scholarly journals A Convenient Co-Dominant Marker for Height-Reducing Ddw1 Allele Useful for Marker-Assisted Selection

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 110 ◽  
Author(s):  
Dmitry Y. Litvinov ◽  
Anastasiya G. Chernook ◽  
Pavel Yu. Kroupin ◽  
Mikhail S. Bazhenov ◽  
Gennady I. Karlov ◽  
...  
Keyword(s):  
Plant Height ◽  
Marker Assisted Selection ◽  
Caps Marker ◽  
Dominant Marker ◽  
Lodging Resistance ◽  
Breeding Programs ◽  
Effective Height ◽  
Allele Status ◽  
Plant Phenotype ◽  
Basic Equipment

Reducing plant height improves lodging resistance and helps to obtain high grain yield under various environmental conditions. So far, the introduction and maintenance of the dwarfing allele of the Ddw1 gene is the most effective height-reducing genetic approach in rye and triticale breeding programs. However, the dominance of the dwarfing Ddw1 allele makes it difficult to select against heterozygous lines for further breeding based on plant phenotype. To assist breeders in the identification of the allele status of the Ddw1 gene, we developed a cleaved amplified polymorphic sequence (CAPS) marker that requires basic equipment and can be easily applied. The CAPS marker was tested on two F2 segregating populations of triticale, and the test showed the association of the Ddw1 genotype with plant height. The application of the marker for marker-assisted selection (MAS) for rye and triticale is discussed in detail.

Molecular mapping of the C locus for presence of pungency in Capsicum

Genome ◽  
2002 ◽  
Vol 45 (4) ◽  
pp. 702-705 ◽  
Author(s):  
Eyal Blum ◽  
Kede Liu ◽  
Michael Mazourek ◽  
Eun Young Yoo ◽  
Molly Jahn ◽  
...  
Keyword(s):  
High Throughput Screening ◽  
Marker Assisted Selection ◽  
Molecular Mapping ◽  
Dominant Gene ◽  
Wild Accession ◽  
Rflp Markers ◽  
Chromosome 2 ◽  
Breeding Programs ◽  
Unique Character ◽  
Capsicum Spp

Pungency owing to the presence of capsaicinoids is a unique character of pepper (Capsicum spp.). Capsaicinoids are produced in the placenta and it has long been known that a single dominant gene, C, is required for pungent genotypes to produce capsaicinoids. We mapped C to pepper chromosome 2 in a cross between a pungent Capsicum frutescens wild accession and a non-pungent Capsicum annuum bell pepper. This position confirmed results from earlier studies. The RFLP marker TG 205 cosegregated with C and two additional RFLP markers were also located within 1 cM. The recessive allele at the C locus is used in breeding programs around the world focused on very diverse germplasm, hence any of these tightly linked markers may be of value as potential sources of useful markers for marker-assisted selection. To demonstrate this point, we developed a PCR-based CAPS (cleaved amplified polymorphic sequence) marker linked to C using the sequence of the Capsicum fibrillin gene located 0.4 cM from C. The use of molecular markers for high-throughput screening for the c allele in pepper breeding programs is discussed.Key words: pepper, pungency, marker-assisted selection.

scholarly journals QTL detection of yield-related traits of cashew

2012 ◽  
Vol 12 (1) ◽  
pp. 60-66 ◽  
Author(s):  
José Jaime Vasconcelos Cavalcanti ◽  
Francisco Herbeth Costa dos Santos ◽  
Fanuel Pereira da Silva ◽  
Cássia Renata Pinheiro
Keyword(s):  
Quantitative Trait ◽  
Marker Assisted Selection ◽  
Interval Mapping ◽  
Phenotypic Variance ◽  
Qtl Detection ◽  
Total Phenotypic Variance ◽  
Breeding Programs ◽  
Multiple Qtl ◽  
Male Flowers ◽  
Selection Of

The identification of quantitative trait loci (QTL) and marker-assisted selection with a view to breeding programs have aroused great interest, including for cashew improvement. This study identified QTL for yield-related traits: nut weight, male and hermaphrodite flowers. The traits were evaluated in 71 F1 genotypes of the cross CCP 1001 x CP 96. The methods of interval mapping and multiple QTL mapping were applied to identify QTL. Eleven QTL were detected: three for nut weight, four for male flowers and four for hermaphrodite flowers. The QTL accounted for 3.79 to 12.98 % of the total phenotypic variance and had phenotypic effects of -31.81 to 34.25 %. The potential for marker-assisted selection of the QTL hf-2f and hf-3m is great and the phenotypic effects and percentage of phenotypic variation higher than of the others.

Sign in / Sign up

Export Citation Format

Share Document