scholarly journals A Convenient Co-Dominant Marker for Height-Reducing Ddw1 Allele Useful for Marker-Assisted Selection

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 110 ◽  
Author(s):  
Dmitry Y. Litvinov ◽  
Anastasiya G. Chernook ◽  
Pavel Yu. Kroupin ◽  
Mikhail S. Bazhenov ◽  
Gennady I. Karlov ◽  
...  
Keyword(s):  
Plant Height ◽  
Marker Assisted Selection ◽  
Caps Marker ◽  
Dominant Marker ◽  
Lodging Resistance ◽  
Breeding Programs ◽  
Effective Height ◽  
Allele Status ◽  
Plant Phenotype ◽  
Basic Equipment

Reducing plant height improves lodging resistance and helps to obtain high grain yield under various environmental conditions. So far, the introduction and maintenance of the dwarfing allele of the Ddw1 gene is the most effective height-reducing genetic approach in rye and triticale breeding programs. However, the dominance of the dwarfing Ddw1 allele makes it difficult to select against heterozygous lines for further breeding based on plant phenotype. To assist breeders in the identification of the allele status of the Ddw1 gene, we developed a cleaved amplified polymorphic sequence (CAPS) marker that requires basic equipment and can be easily applied. The CAPS marker was tested on two F2 segregating populations of triticale, and the test showed the association of the Ddw1 genotype with plant height. The application of the marker for marker-assisted selection (MAS) for rye and triticale is discussed in detail.

scholarly journals Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Doubled Haploid ◽  
Marker Assisted Selection ◽  
Triticum Aestivum L ◽  
Physiological Model ◽  
Yield Potential ◽  
Breeding Programs ◽  
Per Se ◽  
Genomic Regions ◽  
Fertility Traits ◽  
Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

scholarly journals Morphological variation in Paspalum nicorae Parodi accessions, a promising forage

2010 ◽  
Vol 67 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Camila Aparecida de Oliveira dos Reis ◽  
Miguel Dall'Agnol ◽  
Carlos Nabinger ◽  
Maria Teresa Schifino-Wittmann
Keyword(s):  
Plant Height ◽  
Morphological Variation ◽  
Geographical Location ◽  
Rio Grande ◽  
Morphological Characterization ◽  
Leaf Length ◽  
Rio Grande Do Sul ◽  
Breeding Programs ◽  
Grazing Tolerance ◽  
Leaf Hairiness

Paspalum nicorae Parodi is a perennial, apomictic tetraploid forage species, native in the state of Rio Grande do Sul, Brazil, with grazing tolerance and adapted to sandy soils and moderate droughts. As a first step to future breeding, a morphological characterization regarding leaf hairiness, sheath, leaf and central venation color, growing habit, plant height, raceme number and length, and leaf length and width was performed in 53 accessions from Rio Grande do Sul that are part of a Paspalum species breeding program. 35.84% of the accessions had completely hairy leaves, 73.58% had green sheath, 54.71% whitish central venation, 50.94% decumbent habit. Leaf color was estimated by two methods, color chart and colorimeter. The accessions were classified as 76.92% green, 13.45% greenish yellow and 9.62% as grayish green by the first, and 59.62% grayish, 32.69% grayish yellow, 5.77% yellow and 1.92% dark grayish by the second method. Raceme length ranged from 9.40 to 1.30 cm, number of racemes from one to six (48.73% of the accessions had four racemes), leaf length and width from 36.13 to 13.06 cm and 0.67 to 0.36 cm, respectively, and plant height from 115.70 to 29.00 cm. Therefore, a large morphological variation among accessions was detected, with no relation to geographical location but indicating a high potential use in plant breeding programs.

scholarly journals Inheritance of plant height, spike length and number of spikelets per spike in Durum wheat

2007 ◽  
pp. 27-33 ◽  
Author(s):  
Biljana Gorjanovic ◽  
Marija Kraljevic-Balalic
Keyword(s):  
Durum Wheat ◽  
Plant Height ◽  
Combining Ability ◽  
Wheat Breeding ◽  
Specific Combining Ability ◽  
Spike Length ◽  
First Year ◽  
Gene Effects ◽  
Breeding Programs ◽  
Additive Genes

Using the line x tester analysis we studied the combining ability and gene effects of plant height, spike length and number of spikelets per spike in durum wheat. The results of the study show that non-additive genes play more important role than additive genes in the inheritance of plant height, number of spikelets per spike in both years and in inheritance of spike length only in the first year of research. Variety Belfugito, the best general combiner for plant height and number of spikelets per spike, combined well in two best hybrids: Belfugito x Alifen and Belfugito x Yavaros 79, and these hybrids may be used in wheat breeding programs. In the majority of the cases, good specific combining ability (SCA) effects were associated with crosses of two genetically divergent parents having at least one parent as a good general combiner.

scholarly journals Effects of Mutagens on Morphological traits of Psoralea corylifolia-A Medicinally Important Plant

2020 ◽  
Vol 26 (2) ◽  
Author(s):  
Ajey Karan Chaudhari ◽  
Anand Prakash Singh ◽  
B R Chaudhary
Keyword(s):  
Plant Height ◽  
Seed Yield ◽  
Morphological Traits ◽  
Mutation Breeding ◽  
Yield Potential ◽  
Mutagenic Potential ◽  
Breeding Programs ◽  
Methane Sulphonate ◽  
Flowering Initiation ◽  
Yield Quality

Mutation breeding like in other plants can significantly strengthen medicinal plants breeding programs and help to produce novel varieties with higher yield potential and improved yield quality. The dry and healthy seeds of P. corylifolia IC 111228 were subjected to mutagenic treatments namely ethyl methane sulphonate (EMS) and sodium azide (SA). The treatment concentrations 15mM, 30mM, 45mM and 60mM of EMS and 1mM, 2mM, 3mM and 4mM of SA were chosen to evaluate the mutagenic potential in either case. The morphological traits were evaluated in M1 generation viz. plant height, days to flowering initiation, seed yield/plant and 100-seed weight. In EMS treatment 15 mM increased the plant height and seed yield, while in SA treatment 4 mM increased the seed yield/plant and 100-seed yield.

scholarly journals Morphologic Characteristics Variability in Prunus spinosa L. Shrubs Identified in Southern Area of Oltenia, Romania

2018 ◽  
Vol 10 (3) ◽  
pp. 447-451 ◽  
Author(s):  
Sina N. COSMULESCU ◽  
Florentina GAVRILA CALUSARU
Keyword(s):  
Plant Height ◽  
Tree Species ◽  
Morphological Characteristics ◽  
Fruit Tree ◽  
High Variability ◽  
Stem Height ◽  
Breeding Programs ◽  
Trunk Diameter ◽  
Prunus Spinosa ◽  
Morphologic Characteristics

This paper aims to analyse the variability of morphological characteristics of Prunus spinosa L. shrubs, identified and studied in the spontaneous flora in southern Oltenia, Romania (localities Calopar and Gura Văii). The observations made on trunk diameter, stem height, spinosity degree, ability to form basal shoots, have outlined the high variability, which is useful in breeding programs. Variability coefficient ranged between 26.02% and 30.87% for the stem diameter, between 23.48% and 32.32% for the plant height, between 30.93% and 37.81% for canopy diameter. Based on the obtained results, the C31, GV26, GV28, GV29, GV30, GV31, GV33, GV34, GV37 and GV8, GV9, GV27, GV35, C5, C24, C25, C26 and C29 genotypes are recommended for fixing the eroded lands, owing to their very strong, strong and medium ability to form basal shoots; while C10, C12, C23, C27, C28, C30, GV6, GV10, GV24, GV25, GV32 and GV36 genotypes are recommended to be studied as rootstocks in fruit tree species, having in view the low or missing vigor and ability to form basal shoots.

scholarly journals Cloning and Functional Analysis of Dwarf Gene Mini Plant 1 (MNP1) in Medicago truncatula

2020 ◽  
Vol 21 (14) ◽  
pp. 4968
Author(s):  
Shiqi Guo ◽  
Xiaojia Zhang ◽  
Quanzi Bai ◽  
Weiyue Zhao ◽  
Yuegenwang Fang ◽  
...  
Keyword(s):  
Plant Height ◽  
Medicago Truncatula ◽  
Crop Yields ◽  
Genetic Regulation ◽  
Cell Number ◽  
Lodging Resistance ◽  
Dwarf Gene ◽  
Model Legume ◽  
Close Relationship ◽  
Ga Biosynthesis

Plant height is a vital agronomic trait that greatly determines crop yields because of the close relationship between plant height and lodging resistance. Legumes play a unique role in the worldwide agriculture; however, little attention has been given to the molecular basis of their height. Here, we characterized the first dwarf mutant mini plant 1 (mnp1) of the model legume plant Medicago truncatula. Our study found that both cell length and the cell number of internodes were reduced in a mnp1 mutant. Using the forward genetic screening and subsequent whole-genome resequencing approach, we cloned the MNP1 gene and found that it encodes a putative copalyl diphosphate synthase (CPS) implicated in the first step of gibberellin (GA) biosynthesis. MNP1 was highly homologous to Pisum sativum LS. The subcellular localization showed that MNP1 was located in the chloroplast. Further analysis indicated that GA3 could significantly restore the plant height of mnp1-1, and expression of MNP1 in a cps1 mutant of Arabidopsis partially rescued its mini-plant phenotype, indicating the conservation function of MNP1 in GA biosynthesis. Our results provide valuable information for understanding the genetic regulation of plant height in M. truncatula.

scholarly journals Identification of Bna.IAA7.C05 as allelic gene for dwarf mutant generated from tissue culture in oilseed rape

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Hongtao Cheng ◽  
Fenwei Jin ◽  
Qamar U. Zaman ◽  
Bingli Ding ◽  
Mengyu Hao ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Height ◽  
High Throughput Sequencing ◽  
Agronomic Traits ◽  
Crop Improvement ◽  
Dwarf Mutant ◽  
Sequencing Analysis ◽  
Rna Seq ◽  
Dwarf Phenotype ◽  
Lodging Resistance

Abstract Background Plant height is one of the most important agronomic traits in many crops due to its influence on lodging resistance and yield performance. Although progress has been made in the use of dwarfing genes in crop improvement, identification of new dwarf germplasm is still of significant interest for breeding varieties with increased yield. Results Here we describe a dominant, dwarf mutant G7 of Brassica napus with down-curved leaves derived from tissue culture. To explore the genetic variation responsible for the dwarf phenotype, the mutant was crossed to a conventional line to develop a segregating F2 population. Bulks were formed from plants with either dwarf or conventional plant height and subjected to high throughput sequencing analysis via mutation mapping (MutMap). The dwarf mutation was mapped to a 0.6 Mb interval of B. napus chromosome C05. Candidate gene analysis revealed that one SNP causing an amino acid change in the domain II of Bna.IAA7.C05 may contribute to the dwarf phenotype. This is consistent with the phenotype of a gain-of-function indole-3-acetic acid (iaa) mutant in Bna.IAA7.C05 reported recently. GO and KEGG analysis of RNA-seq data revealed the down-regulation of auxin related genes, including many other IAA and small up regulated response (SAUR) genes, in the dwarf mutant. Conclusion Our studies characterize a new allele of Bna.IAA7.C05 responsible for the dwarf mutant generated from tissue culture. This may provide a valuable genetic resource for breeding for lodging resistance and compact plant stature in B. napus.

Genetic Recourses : Evaluation Of The Value Of Some Old Latvian Flax Accessions

2015 ◽  
Vol 2 ◽  
pp. 297
Author(s):  
Zofija Jankauskienė ◽  
Elvyra Gruzdevienė
Keyword(s):  
Plant Height ◽  
Flower Colour ◽  
Vegetation Period ◽  
Fibre Content ◽  
Research Centre ◽  
Breeding Programs ◽  
Experimental Station ◽  
Fibre Yield ◽  
Phenological Phases

In the resent years evaluation and conservation of genetic recourses becomes of great importance. Some of the genotypes could be lost when un-sufficient attention is given to them. Some old (created before WWII) Latvian flax accessions have been investigated at the collection nursery at the Upytė Experimental Station of the Lithuanian Research Centre for Agriculture and Forestry in 2007-2010. Phenological phases, plant height, flower colour, stem, seed and long fibre yield, fibre content and quality (flexibility, strength, quality number), duration of vegetation period, resistance to lodging, etc., have been investigated. The most valuable genotypes have been suggested to be used in further breeding programs.

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