scholarly journals 3D-printed assistive pipetting system for gel electrophoresis for technicians with low acuity vision

BioTechniques ◽  
2020 ◽  
Author(s):  
Alex Viqian Huynh ◽  
Phillip Stein ◽  
Ethan D Buhr

In molecular biology laboratories, many tasks require fine motor control and high acuity vision. For example, lab technicians with visual impairment experience difficulty loading samples into the small wells of a horizontal agarose gel. We have developed a 3D-printable gel loading system which allows technicians with low-contrast vision to load gels correctly. It includes a casting tray, a bridge, and a modified comb. The system provides a high-contrast visual field to improve visibility, and the bridge allows pipette tips to be inserted at the correct location and only to the correct depth. The necessary computer files for printing this device are freely available to increase the accessibility of molecular biology laboratories to people with visual impairment.


Sensors ◽  
2019 ◽  
Vol 19 (15) ◽  
pp. 3416 ◽  
Author(s):  
Aleksandra Kowalska ◽  
Robert Banasiak ◽  
Andrzej Romanowski ◽  
Dominik Sankowski

Presently, Electrical Capacitance Tomography (ECT) is positioned as a relatively mature and inexpensive tool for the diagnosis of non-conductive industrial processes. For most industrial applications, a hand-made approach for an ECT sensor and its 3D extended structure fabrication is used. Moreover, a hand-made procedure is often inaccurate, complicated, and time-consuming. Another drawback is that a hand-made ECT sensor’s geometrical parameters, mounting base profile thickness, and electrode array shape usually depends on the structure of industrial test objects, tanks, and containers available on the market. Most of the traditionally fabricated capacitance tomography sensors offer external measurements only with electrodes localized outside of the test object. Although internal measurement is possible, it is often difficult to implement. This leads to limited in-depth scanning abilities and poor sensitivity distribution of traditionally fabricated ECT sensors. In this work we propose, demonstrate, and validate experimentally a new 3D ECT sensor fabrication process. The proposed solution uses a computational workflow that incorporates both 3D computer modeling and 3D-printing techniques. Such a 3D-printed structure can be of any shape, and the electrode layout can be easily fitted to a broad range of industrial applications. A developed solution offers an internal measurement due to negligible thickness of sensor mount base profile. This paper analyses and compares measurement capabilities of a traditionally fabricated 3D ECT sensor with novel 3D-printed design. The authors compared two types of the 3D ECT sensors using experimental capacitance measurements for a set of low-contrast and high-contrast permittivity distribution phantoms. The comparison demonstrates advantages and benefits of using the new 3D-printed spatial capacitance sensor regarding the significant fabrication time reduction as well as the improvement of overall measurement accuracy and stability.



2011 ◽  
Vol 32 (5) ◽  
pp. 1924-1933 ◽  
Author(s):  
A.M. Reimer ◽  
R.F.A. Cox ◽  
M.W.G. Nijhuis-Van der Sanden ◽  
F.N. Boonstra


2020 ◽  
Vol 2 (2) ◽  
pp. 106-113
Author(s):  
Anik Lestariningrum ◽  
Isfauzi Hadi Nugroho ◽  
Agustia Budiarti

ABSTRAK : Permasalahan yang terjadi dalam pengembangan kemampuan pada anak usia dini terkait kemampuan motorik halus  anak terkait  koordinasi jari tangan dalam kaitannyan dengan kelenturan jari tangan menyebabkan anak mengalami kesulitan dalam menggengam alat tulis serta alat makan. Untuk membantu permasalahan tersebut kegiatan bermain meremas  koran menjadi alternatif solusi pembelajarannya.    Pemanfaatkan koran bekas sebagai alternatif akan menstimulasi perkembangan motorik halus . Tujuan dari penelitian untuk memaparkan  kegiatan bermain meremas koran untuk mengembangkan kemampuan motorik halus pada anak. Desain penelitian deskriptif kualitatif dengan teknik penilaian observasi, dan dokumentasi. Subjek penelitian ini adalah anak Kelompok Bermain Ceria Desa Kudu Kecamatan Kertosono Kabupaten Nganjuk yang berjumlah 10 siswa, dari hasil penelitian diperoleh data sebanyak 8 anak mendapat predikat berkembang  sangat baik, dan 2 anak mendapat predikat mulai berkembang. Hasil penelitian ini direkomendasikan supaya kegiatan bermain meremas koran dikembangkan untuk aspek lain supaya seluruh aspek bisa berkembang dengan kegiatan beragam dan menarik. ABSTRACT : Problems that occur in developing abilities in early childhood are related to children's fine motor skills related to finger coordination in relation to finger flexibility which causes children to experience difficulty in holding stationery and eating utensils. To help with this problem, playing squeezing newspapers is analternative learning solution. Using old newspapers as an alternative will stimulate fine motor development. The purpose of this research is to describe the activity of playing squeezing newspapers to develop fine motor skills in children. Descriptive qualitative research design with assessment techniques,observation and documentation. The research instrument used was the observation and interview guidelines which were analyzed descriptively by the analysis of the Miles and Hubermen model. The subjects of this study were 10 children of the Cheerful Play Group in Kudu Village, Kertosono District, Nganjuk Regency. The results showed that 8 children received the predicate of developing very well, and 2 children received the title of starting to develop. The conclusion in this study is the activity of squeezing newspapers can develop the motor skills of early childhood.



2019 ◽  
Author(s):  
Gaurav Byagathvalli ◽  
Aaron F. Pomerantz ◽  
Soham Sinha ◽  
Janet Standeven ◽  
M. Saad Bhamla

The centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional centrifuges are often inaccessible outside of conventional laboratory settings, such as remote field sites, require a constant external power source, and can be prohibitively costly in resource-limited settings and STEM-focused programs. Here we present the 3D-Fuge, a 3D-printed hand-powered centrifuge, as a novel alternative to standard benchtop centrifuges. Based on the design principles of a paper-based centrifuge, this 3D-printed instrument increases the volume capacity to 2 mL and can reach hand-powered centrifugation speeds up to 6,000 rpm. The 3D-Fuge devices presented here are capable of centrifugation of a wide variety of different solutions such as spinning down samples for biomarker applications and performing nucleotide extractions as part of a portable molecular lab setup. We introduce the design and proof-of-principle trials that demonstrate the utility of low-cost 3D printed centrifuges for use in remote and educational settings.



2019 ◽  
Vol 6 ◽  
pp. 238212051984385 ◽  
Author(s):  
Valerie Juniat ◽  
Rabia Bourkiza ◽  
Aditi Das ◽  
Raja Das-Bhaumik ◽  
Panagiota Founti ◽  
...  

Introduction: Simulation activities are valuable teaching aids for understanding about living with visual impairment (VI). Our medical students used low-vision simulation spectacles (Sim-specs) to enable learning about VI. Methods: Students made tea and filled dosette boxes using Sim-specs simulating central visual loss (age-related macular degeneration) and navigated using Sim-specs simulating peripheral visual loss (glaucoma). Facilitators recorded errors made for each task. Students completed questionnaires to grade the tasks’ difficulty on a 4-point Likert-type scale. The students also participated in focus groups to discuss how their approach to working with patients may change following this training. Results: In total, 252 out of 254 students participated. Central visual loss provided the greatest challenge when undertaking fine motor skilled activity (dosette box). Highest average number of errors made was for dosette box task (0.70 error), followed by navigation (0.59), then making tea task (0.34). Students scored the most difficult task on average as the dosette box task (3.23 Likert-type points), followed by navigation (2.97), then making tea task (2.63). Our students have shown learning in recognising the challenges of VI and have adapted their approach to patients. Conclusions: Simulation activities are valuable additions to the undergraduate curriculum. Such activities can potentially enable greater empathy for our visually impaired patients.



2010 ◽  
Vol 27 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Yan Wang ◽  
Kanxing Zhao ◽  
Xiaoyan Yang ◽  
Jicang He ◽  
Wei Wang


2004 ◽  
Vol 20 (5) ◽  
pp. 689-692 ◽  
Author(s):  
Joseph Anthony J Tumbocon ◽  
Palanisamy Suresh ◽  
Allan Slomovic ◽  
David S Rootman


Pharmaceutics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 172 ◽  
Author(s):  
Atheer Awad ◽  
Aliya Yao ◽  
Sarah J. Trenfield ◽  
Alvaro Goyanes ◽  
Simon Gaisford ◽  
...  

Visual impairment and blindness affects 285 million people worldwide, resulting in a high public health burden. This study reports, for the first time, the use of three-dimensional (3D) printing to create orally disintegrating printlets (ODPs) suited for patients with visual impairment. Printlets were designed with Braille and Moon patterns on their surface, enabling patients to identify medications when taken out of their original packaging. Printlets with different shapes were fabricated to offer additional information, such as the medication indication or its dosing regimen. Despite the presence of the patterns, the printlets retained their original mechanical properties and dissolution characteristics, wherein all the printlets disintegrated within ~5 s, avoiding the need for water and facilitating self-administration of medications. Moreover, the readability of the printlets was verified by a blind person. Overall, this novel and practical approach should reduce medication errors and improve medication adherence in patients with visual impairment.



2021 ◽  
Author(s):  
M. Rauchenzauner ◽  
K. Schiller ◽  
M. Honold ◽  
I. Baldissera ◽  
R. Biedermann ◽  
...  

Abstract Background Cerebral palsy (CP) is the most common motor impairment in childhood and often accompanied by a broad spectrum of comorbidities. Data are sparse concerning visual impairment (VI) and functional classification among CP children. Objective The objective of this study was to analyze the prevalence of VI among children with CP and to investigate a possible association between VI and Gross Motor Function Classification System (GMFCS) and the Bimanual Fine Motor Function (BFMF). Methods In this hospital-based study, records of 200 children with CP aged 2 to 17 years were reviewed. Results Overall, VI was found in 59.5% of children with CP. Prevalence of VI was higher when compared with non-CP children. A correlation between GMFCS as well as BFMF and severity of VI was found. Children with severe CP were at greater risk for severe VI, especially cerebral VI compared with children with mild CP. Conclusion VI is a significant problem in children with CP and is correlated with motor function. Children with CP should undergo detailed ophthalmologic and orthoptic assessment to enable early intervention.



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