<p>Synergistic Cytotoxic Effect from Combination of Wedelolactone and Cisplatin in HeLa Cell Line: A Novel Finding</p>

As one of the leading causes of death in worldwide, cervical cancer requires the effective therapies to reduce its mortality rate. One of the chemotherapy agents that frequently used in the treatment is cisplatin. However, due to drug resistance and its side effects, an agent that can be combined with cisplatin is needed. Parijoto fruit (Medinilla speciosa Reinw.ex.Bl) contains secondary metabolites compounds that have potential as anticancer. The study aims to determine the cytotoxic effect of methanol extract of Parijoto fruit calculated from the IC50 value and the synergicity of the combinational treatment with cisplatin evaluated from the Combination Index (CI) value and its cell viability by using MTT assay. Results showed that methanol extract of Parijoto fruit (MEP) performed cytotoxic effect on HeLa cell line with IC50 of 209.6 μg/mL while the value of IC50 of cisplatin against HeLa cells amounted to 12.8 μg/mL. The combination of 26.205 ppm (1/8 IC50) of MEP and 1.601 ppm (1/8 IC50) of Cisplatin performed synergistic effect on HeLa cell line with the CI value of 0.69. From the above results, it can be concluded that MEP is potential as co-chemotherapy agent based on the synergistic cytotoxicity effect with cisplatin.Keyword: cytotoxic, Medinilla speciosa, cisplatin, co-chemotherapy, MTT

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The Cytotoxic Effect of Methanolic Extract of Pyracanthacoccinea M. Roemer Fruit on Hela Cell Line, Antioxidant Capacities and Total Phenol Contents of Methanolic and Aquatic Extract of this Fruit

Biomedical & Pharmacology Journal ◽

10.13005/bpj/564 ◽

2015 ◽

Vol 8 (SEMAR) ◽

pp. 99-103 ◽

Cited By ~ 1

Author(s):

LEILA VAHABI ◽

RAMESH MONAJEMI ◽

SEYED AHMAD HOSSEINI

Keyword(s):

Hela Cell ◽

Cell Line ◽

Cytotoxic Effect ◽

Methanolic Extract ◽

Total Phenol ◽

Hela Cell Line ◽

Antioxidant Capacities

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AKTIVITAS SITOTOKSIK EKSTRAK ETANOLIK HERBA CIPLUKAN (Physalis angulata L.) PADA SEL KANKER LEHER RAHIM HeLa MELALUI MODULASI EKSPRESI PROTEIN p53

Farmasains Jurnal Farmasi dan Ilmu Kesehatan ◽

10.22219/far.v1i2.1163 ◽

2012 ◽

Vol 1 (2) ◽

Author(s):

Andita Pra Darma ◽

Rosana Anna Ashari ◽

Perdana Adhi Nugroho ◽

Ameilinda Monikawati ◽

Ilham Agusta Fauzi ◽

...

Keyword(s):

Cervical Cancer ◽

Cell Proliferation ◽

Hela Cell ◽

Cell Line ◽

Cytotoxic Effect ◽

Ethanolic Extract ◽

Cytotoxic Agents ◽

Hela Cell Line ◽

Potential Activity ◽

Physalis Angulata

Cervical cancer is one of leading cause of cancer death in women in the developing countries. One of the strategy to prevent cervical cancer based on cytotoxic agents are now being developed. Ciplukan (Physalis angulata L.) is one of potential plant as chemopreventive agent due to Physalin and Withangulatin constituent in this plant. This study was aimed to know cytotoxic effect of ciplukan ethanolic extract (CEE) in human cervical carcinoma Hela cell line. Evaluation of cell viability value was determined using MTT assay. The expression of p53 as cell proliferation regulator was observed with immunocytochemistry assay. Ethanolic extract of ciplukan showed cytotoxic effect in HeLa cell line with IC50 of 158 µg/ml. Further observation of cell proliferation regulator showed that CEE induces expression of p53 that inhibit cell proliferation. The result showed that CEE has potential activity to be developed as anticancer agent in human cervical cancer.

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Identification and Expression Analysis of CD73 Inhibitors in Cervical Cancer

Medicinal Chemistry ◽

10.2174/1573406416666200925141703 ◽

2020 ◽

Vol 16 ◽

Author(s):

Jamshed Iqbal ◽

Ayesha Basharat ◽

Sehrish Bano ◽

Syed Mobasher Ali Abid ◽

Julie Pelletier ◽

...

Keyword(s):

Cell Cycle ◽

Cervical Cancer ◽

Western Blot ◽

Hela Cell ◽

Cell Line ◽

Cell Apoptosis ◽

Immunofluorescence Staining ◽

Cell Cycle Analysis ◽

Hela Cell Line ◽

Cell Line Hela

Aims: The present study was conducted to examine the inhibitory effects of synthesized sulfonylhydrazones on the expression of CD73 (ecto-5′-NT). Background: CD73 (ecto-5′-NT) represents the most significant class of ecto-nucleotidases which are mainly responsible for dephosphorylation of adenosine monophosphate to adenosine. Inhibition of CD73 played an important role in the treatment of cancer, autoimmune disorders, precancerous syndromes, and some other diseases associated with CD73 activity. Objective: Keeping in view the significance of CD73 inhibitor in the treatment of cervical cancer, a series of sulfonylhydrazones (3a-3i) derivatives synthesized from 3-formylchromones were evaluated. Methods: All sulfonylhydrazones (3a-3i) were evaluated for their inhibitory activity towards CD73 (ecto-5′-NT) by the malachite green assay and their cytotoxic effect was investigated on HeLa cell line using MTT assay. Secondly, most potent compound was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis. After that, CD73 mRNA and protein expression were analyzed by real-time PCR and Western blot. Results: Among all compounds, 3h, 3e, 3b, and 3c were found the most active against rat-ecto-5′-NT (CD73) enzyme with IC50 (µM) values of 0.70 ± 0.06 µM, 0.87 ± 0.05 µM, 0.39 ± 0.02 µM and 0.33 ± 0.03 µM, respectively. These derivatives were further evaluated for their cytotoxic potential against cancer cell line (HeLa). Compound 3h and 3c showed the cytotoxicity at IC50 value of 30.20 ± 3.11 µM and 86.02 ± 7.11 µM, respectively. Furthermore, compound 3h was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis which showed promising apoptotic effect in HeLa cells. Additionally, compound 3h was further investigated for its effect on expression of CD73 using qRT-PCR and western blot. Conclusion: Among all synthesized compounds (3a-3i), Compound 3h (E)-N'-((6-ethyl-4-oxo-4H-chromen-3-yl) methylene)-4-methylbenzenesulfonohydrazide was identified as most potent compound. Additional expression studies conducted on HeLa cell line proved that this compound successfully decreased the expression level of CD73 and thus inhibiting the growth and proliferation of cancer cells.

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Molecular events after antisense inhibition of hMSH2 in a HeLa cell line

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/s1383-5718(98)00108-9 ◽

1998 ◽

Vol 418 (2-3) ◽

pp. 61-71 ◽

Cited By ~ 6

Author(s):

Ying Qian ◽

Yingnian Yu ◽

Xingruo Cheng ◽

Jianhong Luo ◽

Haiyang Xie ◽

...

Keyword(s):

Hela Cell ◽

Cell Line ◽

Antisense Inhibition ◽

Hela Cell Line ◽

Molecular Events

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Synthesis, structural analysis, solution equilibria and biological activity of rhodium(iii) complexes with a quinquedentate polyaminopolycarboxylate

RSC Advances ◽

10.1039/c6ra26199j ◽

2017 ◽

Vol 7 (9) ◽

pp. 5282-5296 ◽

Cited By ~ 5

Author(s):

Marija S. Jeremić ◽

Hubert Wadepohl ◽

Vesna V. Kojić ◽

Dimitar S. Jakimov ◽

Ratomir Jelić ◽

...

Keyword(s):

Biological Activity ◽

Structural Analysis ◽

Antitumor Activity ◽

Hela Cell ◽

Cell Line ◽

Hela Cell Line ◽

Solution Equilibria ◽

Line P

Two new Rh(iii)–ed3a complexes [Rh(ed3a)(OH2)]·H2O and Na[Rh(ed3a)Cl]·H2O have shown good antitumor activity, especially against HeLa cell line.

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Exploring the potential of imines as antiprotozoan agents with focus on t. Brucei and p. Falciparum

10.21504/10962/62235 ◽

2018 ◽

Author(s):

◽

Kola Augustus Oluwafemi

Keyword(s):

Hela Cell ◽

Cell Line ◽

Special Focus ◽

Hela Cell Line ◽

Free Energies ◽

Imino Group ◽

Design Synthesis ◽

Lead Compounds ◽

Pyridine Moiety ◽

Nmr Study

This work focuses on the design, synthesis and evaluation of imine-containing heterocyclic and acyclic compounds with special focus on their bioactivity against parasitic protozoans (P. falciparum and T. brucei) - given the context of drug resistance in the treatment of malaria and Human African sleeping sickness and the fact that several bioactive organic compounds have been reported to possess the imino group. Starting from 2-aminopyridine, novel #-alkylated-5-bromo-7-azabenzimidazoles and substituted 5-bromo-1-(carbamoylmethy)-7-azabenzimidazole derivatives were prepared, and their bioactivity against parasitic protozoans was assessed. NMR spectra of the substituted 5- bromo-1-(carbamoylmethy)-7-azabenzimidazole derivatives exhibited rotational isomerism, and a dynamic NMR study was used in the estimation of the rate constants and the free- energies of activation for rotation. The free-energy differences between the two rotamers were determined and the more stable conformations were predicted. Novel 2-phenyl-7-azabenzimidazoles were also synthesised from 2-aminopyridine. A convenient method for the regioselective formylation of 2,3-diaminopyridines into 2-amino- 7-(benzylimino)pyridine analogues of 2-phenyl-7-azabenzimidazole was developed, and some of the resulting imino derivatives were hydrogenated to verify the importance of the imino moiety for bioactivity. The 2-phenyl-7-azabenzimidazoles and the 2-amino-7- (benzylimino)pyridine analogues were screened for their anti-protozoal activity and their cytotoxicity level was determined against the HeLa cell line. In order to validate the importance of the pyridine moiety, novel #-(phenyl)-2- hydroxybenzylimines, #-(benzyl)-2-hydroxybenzylimines and (±)-trans-1,2-bis[2- hydroxybenzylimino]cyclohexanes were also synthesized and screened for activity against the parasitic protozoans and for cytotoxicity against the HeLa cell line. The biological assay results indicated that these compounds are not significantly cytotoxic and a good number of them show potential as lead compounds for the development of new malaria and trypanosomiasis drugs.

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