Background:
Biosynthesis of gold nanoparticles from medicinal plants has become an
interesting strategy in biomedical research due to its exclusive properties including less toxic cellular
level through its ecofriendly biological function.
Objective:
To examine the anti-lipid accumulation effect of spherical gold nanoparticles (size 10-20
nm) synthesized from Dendropanax morbifera Léveille (D-AuNPs) in both 3T3-L1 and HepG2 cells.
Method:
3T3-L1 preadipocytes and HepG2 hepatocytes were stimulated with cocktail media to
generate obese and fatty liver disease models. Cell cytotoxicity and cell proliferation assays were
performed in adipocytes at different stages of growth. An anti-lipid accumulation assay was performed
in 3T3-L1 obese and HepG2 fatty liver models using different doses of D-AuNPs. Expression
of adipogenic genes of PPARγ, CEBPα, Jak2, STAT3, and ap2 and hepatogenic genes PPARα,
FAS, and ACC was measured by real-time PCR. In addition, protein expression of PPARγ and
CEBPα was evaluated by immunoblotting assay.
Result:
We found that D-AuNPs (size 10–20 nm) at concentrations up to 100 µg/ml were nontoxic
to 3T3-L1 and HepG2 at post-confluent and mature stages. In addition, pretreatment of D-AuNPs at
post-confluent stage reduced triglyceride content. In addition, the adipogenesis process was negatively
controlled by D-AuNPs, with downregulated PPARγ, CEBPα, Jak2, STAT3, and ap2 expression
in 3T3-L1 cells and FAS and ACC levels in HepG2 cells.
Conclusion:
These data indicated that D-AuNPs exert antiadipogenic properties. We hypothesize
that Dendropanax contains a large amount of phenolic compound that coats the surface of gold
nanoparticles and has the ability to reduce the excess amount of lipid in both cell lines.
Ethyl 2-[2,3,4-Trimethoxy-6-(1-Octanoyl)Phenyl] Acetate (TMPA) Ameliorates Lipid Accumulation by Disturbing the Combination of LKB1 with Nur77 and Activating the AMPK Pathway in HepG2 Cells and Mice Primary Hepatocytes
