Background:
Current gene therapy vectors such as viral, non-viral, and bacterial vectors,
which are used for cancer treatment, but there are certain safety concerns and stability issues
of these conventional vectors. Exosomes are the vesicles of size 40-100 nm secreted from multivesicular
bodies into the extracellular environment by most of the cell types in-vivo and in-vitro.
As a natural nanocarrier, exosomes are immunologically inert, biocompatible, and can cross biological
barriers like the blood-brain barrier, intestinal barrier, and placental barrier.
Objective:
This review focusses on the role of exosome as a carrier to efficiently deliver a gene for
cancer treatment and diagnosis. The methods for loading of nucleic acids onto the exosomes, advantages
of exosomes as a smart intercellular shuttle for gene delivery and therapeutic applications as
a gene delivery vector for siRNA, miRNA and Clustered Regularly Interspaced Short Palindromic
Repeats (CRISPR) and also the limitations of exosomes as a gene carrier are all reviewed in this article.
Methods:
Mostly, electroporation and chemical transfection are used to prepare gene loaded exosomes.
Results:
Exosome-mediated delivery is highly promising and advantageous in comparison to the
current delivery methods for systemic gene therapy. Targeted exosomes, loaded with therapeutic
nucleic acids, can efficiently promote the reduction of tumor proliferation without any adverse effects.
Conclusion:
In the near future, exosomes can become an efficient gene carrier for delivery and a
biomarker for the diagnosis and treatment of cancer.
Nonviral gene therapy in vivo with PAM-RG4/apoptin as a potential brain tumor therapeutic