scholarly journals Intermediate resistance to nematode Meloidogyne paranaensis in Híbrido de Timor coffee genotypes

2021 ◽  
pp. 628-633
Author(s):  
Gustavo Hiroshi Sera ◽  
Lucas Eduardo Fernandes ◽  
Inês Cristina de Batista Fonseca ◽  
Dhalton Shiguer Ito ◽  
Luciana Harumi Shigueoka ◽  
...  
Keyword(s):  
Germplasm Bank ◽  
Breeding Programs ◽  
Resistance Sources ◽  
Final Population ◽  
Randomized Design ◽  
Intermediate Resistance ◽  
Completely Randomized Design ◽  
Moderate Resistance ◽  
Different Levels ◽  
Reproduction Factor

The aim of this study was to prove that Híbrido de Timor (HdT) accessions are resistance sources to Meloidogyne paranaensis. Two experiments were carried out in a greenhouse at IDR-Paraná (Londrina, Paraná State, Brazil). Open pollinated fruits were harvested from 10 accessions (HdT UFV 380-05, HdT UFV 408-10, HdT UFV 408-11, HdT UFV 408-28, HdT UFV 428-04, HdT UFV 439-14, HdT UFV 443-08, HdT UFV 445-70, HdT UFV 446-138, HdT UFV 448-75) from the EPAMIG/ UFV germplasm bank. Seeds of these accessions were sown to obtain seedlings to test the resistance to M. paranaensis. For each experiment, a completely randomized design was used with 11 treatments, eight replications and one plant per plot. Cultivar Mundo Novo IAC 376-4 was the susceptible check. Seedlings with three to four pairs of leaves were transplanted into plastic cups with a capacity of 700 mL and 1200 eggs. The J2 of M. paranaensis (IP) were inoculated after one month. The assessments were performed 134 days after inoculation, when the data of the number of eggs and J2 per gram of roots and the final population of nematodes (FP) were obtained. The reproduction factor (RF) was calculated using the formula: RF = IP / FP. To classify the resistance levels of the genotypes, the reproduction factor reduction (RFR) was used. It classified the levels from highly resistant to highly susceptible. Different levels of intermediate resistance between the accesses were observed, highlighting HdT UFV 408-28 that presented moderate resistance to M. paranaensis. HdT UFV 408-28 showed a high percentage of plants with intermediate resistance, which can be used in breeding programs aimed at resistance to M. paranaensis

scholarly journals Resistance to nematode Meloidogyne paranaensis in Arabica coffee genotypes introgressed with Coffea liberica

2020 ◽  
pp. 1236-1241
Author(s):  
Gustavo H. Sera ◽  
Filipe G. Carvalho ◽  
Inês C. de B. Fonseca ◽  
Luciana H. Shigueoka ◽  
Santino A. da Silva ◽  
...  
Keyword(s):  
Germplasm Bank ◽  
Breeding Programs ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Mother Plants ◽  
Completely Randomized Design ◽  
Set Up ◽  
Moderately Resistant ◽  
Reproduction Factor ◽  
Coffea Liberica

The aim of this study was to prove that Arabica coffee introgressed with C. liberica, have resistance to Meloidogyne paranaensis (Mp). Open pollinated fruits were harvested from mother plants of 29 Arabica coffee genotypes from the IAPAR germplasm bank. Seeds were collected from the fruits and were sown to obtain seedlings to test the resistance to Mp. The experiment was set up in a completely randomized design with 29 coffee genotypes, 8 replications, and one plant per plot. Cultivars Catuaí Vermelho IAC 99 and IPR 100 were used as susceptible and resistant checks, respectively. Seedlings with three to four pairs of leaves were inoculated with 1,400 eggs and juveniles J2 of Mp (IP). At 120 days after inoculation, seedlings were evaluated by counting the nematodes per gram of roots, and the final nematodes population was obtained (FP). The reproduction factor (RF) was calculated using the formula: RF = FP/IP. The reproduction factor reduction was used to classify the resistance levels of genotypes, which were classified as highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) an highly susceptible (HS). All genotypes differed from Catuaí in resistance factor (RF), five of which did not differ from IPR 100 for RF, and only the line IAPAR 15242 had RF < 1.0. Out of 28 Arabica genotypes introgressed with C. liberica, five HR, 11 R, 11 MR and one MS were identified. However, only IAPAR 15242 and IPR 100 were classified as HR and presented 100% of HR plants, but only the first showed an RF < 1.0. Results revealed that these Arabica coffee genotypes with introgression of C. liberica genes have great potential to be used in breeding programs and they are a new alternative as a source of resistance.

scholarly journals Arabica coffee accessions originated from Ethiopia with resistance to nematode Meloidogyne paranaensis

2020 ◽  
pp. 1209-1213
Author(s):  
Melina Marques Holderbaum ◽  
Dhalton Shiguer Ito ◽  
Débora Cristina Santiago ◽  
Luciana Harumi Shigueoka ◽  
Lucas Eduardo Fernandes ◽  
...  
Keyword(s):  
Coffea Arabica ◽  
Germplasm Bank ◽  
Heterozygous Condition ◽  
Arabica Coffee ◽  
Final Population ◽  
Randomized Design ◽  
Susceptible Control ◽  
Completely Randomized Design ◽  
Reproduction Factor

The aim of this study was to evaluate the resistance of Ethiopian Coffea arabica from IAPAR’s germplasm bank to Meloidogyne paranaensis. Two experiments were carried out in a greenhouse in IAPAR, Londrina, Paraná State, Brazil. A completely randomized design was used with 15 treatments, eight replications and one plant per plot. 14 accessions of C. arabica from Ethiopia were evaluated and the cultivar Mundo Novo IAC 376-4 was used as a susceptible control. Seedlings with three to four pairs of leaves were transplanted into plastic cups with a capacity of 700 mL and 1200 eggs and/or J2 of M. paranaensis (IP) were inoculated after one month. The evaluations were performed 130 days after inoculation, when the data of the number of eggs and J2 per gram of roots, the final population of nematodes (FP) were obtained and the reproduction factor (RF) was calculated using the formula: RF = IP / FP. To classify the resistance levels of the genotypes, the reduction of the reproduction factor (RRF) was used. The Ethiopian accessions were classified from highly resistant to highly susceptible. Most of the Ethiopian coffees were highly resistant to M. paranaensis. The genotype Geisha also showed resistance, but is still in heterozygous condition.

scholarly journals Reaction of melon genotypes to Meloidogyne incognita and Meloidogyne javanica

2016 ◽  
Vol 46 (1) ◽  
pp. 111-115
Author(s):  
Guilherme Matos Martins Diniz ◽  
Willame dos Santos Candido ◽  
Renato Silva Soares ◽  
Lucas da Silva Santos ◽  
Marcus Vinícius Marín ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Santa Cruz ◽  
Breeding Programs ◽  
Northeast Region ◽  
Resistant Cultivars ◽  
Randomized Design ◽  
Susceptible Control ◽  
Completely Randomized Design ◽  
Reproduction Factor

ABSTRACT Melon is one of the most economically important crops cultivated in Brazil, especially in the Northeast region. However, as its cultivation increases, phytosanitary problems arise, including those caused by nematodes, which are most effectively controlled using resistant cultivars. This study aimed at assessing the reaction of muskmelon genotypes, in terms of resistance to Meloidogyne incognita and M. javanica infestation. The experiment was conducted under greenhouse conditions using a completely randomized design, in a 2 x 15 factorial scheme, with six replications. A total of 15 muskmelon genotypes were evaluated and the 'Santa Cruz Kada' tomato was used as a susceptible control. The total number of eggs and juvenile nematodes in the roots and the reproduction factor were used to assess the genotype reaction. None of the genotypes was resistant to M. incognita. Eight genotypes were classified as resistant to M. javanica and promising for use in melon breeding programs.

scholarly journals Influence of Meloidogyne incognita race 1 on the development of clones of Coffea canephora, variety “Jequitibá Incaper 8122”

2020 ◽  
Vol 87 ◽  
Author(s):  
Lilian Katiany Castello Rabello Zinger ◽  
Fernando Domingo Zinger ◽  
Fábio Ramos Alves ◽  
Waldir Cintra de Jesus Junior ◽  
Angelo Oliveira Gonçalves ◽  
...  
Keyword(s):  
Coffea Canephora ◽  
Root Knot Nematode ◽  
Fresh Matter ◽  
Second Stage ◽  
Final Population ◽  
Randomized Design ◽  
Race 1 ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Reproduction Factor

ABSTRACT: Root-knot nematode is one of the most important phytosanitary problems for Conilon coffee, as it reduces productivity and is difficult to handle. We aimed at studying the infectivity and damage caused by M. incognita race 1 in the “Jequitibá Incaper 8122” intermediate maturity coffee variety. The experiment was conducted in a greenhouse, in completely randomized design, with five replicates. The clones composing the variety “Jequitibá Incaper 8122” were inoculated with 2,000 eggs + second-stage juveniles of M. incognita race 1. Uninoculated plants were the control. Evaluations were performed 180 days after inoculation, considering the plant height (H), stem diameter (SD), number of leaves (NOL), leaf area (LA), number of plagiotropic branches (NPB), number of nodes (NN), chlorophyll content (CHLO), shoot dry matter (SDM), root fresh matter (RFM), final population (FNP), and reproduction factor (NRF). The nematode reduced NOL in clones 208 and 209, NRF in clones 201, 203, 207 and 208, NN in clones 203, 207, 208 and 209, CHLO in clones 201, 204, 206, 207 and 209, SDM in clones 201, 203, 204 and 205 and RFM in clones 205 and 207. M. incognita race 1 FNP and NRF were larger in clones 208, 201, 207 and 203. Clone 202 had FNP and NRF equal to zero, being immune to the nematode. Clone 206 presented the lowest NRF value among clones parasitized by M. incognita.

scholarly journals Seed treatment for management of Meloidogyne javanica in soybean

2017 ◽  
Vol 38 (5) ◽  
pp. 2995 ◽  
Author(s):  
Adriely Alves de Almeida ◽  
Vinicius Hicaro Frederico Abe ◽  
Ricardo Marcelo Gonçalves ◽  
Maria Isabel Balbi-Peña ◽  
Débora Cristina Santiago
Keyword(s):  
Seed Treatment ◽  
Meloidogyne Javanica ◽  
Vitro Assay ◽  
Juvenile Mortality ◽  
Second Stage ◽  
Final Population ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Soybean Plants

The objective of this study was to evaluate the efficacy of the active ingredient abamectin, either in isolation or in combination with fungicides and insecticides formulated for the industrial seed treatment, on the population of Meloidogyne javanica, tested under greenhouse and in vitro conditions. In both tests, the combination of the following treatments was assessed: abamectin; thiamethoxam; fludioxonil + metalaxyl-M + thiabendazole. Water was used as control for the in vitro assay, whereas under greenhouse conditions, controls were inoculated and not inoculated with M. javanica. The tests were conducted in a completely randomized design with six (in vitro) and ten (greenhouse) replicates. For in vitro studies the effect on hatching, motility, and mortality of juveniles of M. javanica was evaluated. Under greenhouse conditions, the soybean ‘BRS 133’ seeds were treated, and at 15, 30, and 60 days after inoculation (DAI) with M. javanica, plant measurements were recorded. The penetration of second stage juveniles (J2) was also evaluated at 15 DAI. At 30 DAI, galls, egg masses, nematodes/g of root, and final population were evaluated. At 60 DAI, the final population of nematodes in the roots was quantified. The treatments containing abamectin were the most effective in diminishing the hatching of juveniles. All treatments had an effect on nematode motility when compared to the control, and in the treatment containing only abamectin, total juvenile mortality was observed. In greenhouse conditions, at 15 DAI, the treated soybean plants had the highest root mass and shoot length, differing statistically from the inoculated control. All treatments reduced the number of nematodes per gram of root, differing from the control. At 30 DAI, treatment efficiency was observed in reducing the final population of M. javanica, particularly the treatments using abamectin, and abamectin + thiamethoxam + fludioxonil + metalaxyl-M + thiabendazole. However, at 60 DAI, the effect of the treatments on the population of M. javanica did not persist.

scholarly journals Nutrient and inorganic solute (Na+ and Cl-) content in green onion plants under hydroponic cultivation using brackish water

2020 ◽  
Vol 44 ◽  
Author(s):  
Carlos Donato da Silva Souza ◽  
Geronimo Ferreira da Silva ◽  
Sirleide Maria de Menezes ◽  
José Edson Florentino de Morais ◽  
José Amilton Santos Júnior ◽  
...  
Keyword(s):  
Nutrient Solution ◽  
Brackish Water ◽  
Hydroponic System ◽  
Brackish Waters ◽  
Randomized Design ◽  
Hydroponic Cultivation ◽  
Salinity Levels ◽  
Completely Randomized Design ◽  
Harmful Effects ◽  
Different Levels

ABSTRACT Cultivation using brackish waters can result in nutritional and metabolic imbalances in several plant species, consequently reducing the production of dry matter (DM) and accumulation of toxic ions (Na+ and/or Cl-) in plants. We evaluated the DM production, and nutrient and inorganic solute (Na+ and Cl-) content in green onion plants (cv. Todo Ano Evergreen - Nebuka) under different levels of nutrient solution salinity in combination with circulation frequencies of this solution. Two experiments were conducted in a hydroponic system, using a completely randomized design, in a 6 × 2 factorial scheme, with five replicates: six levels of nutrient solution salinity (1.5, 3.0, 4.5, 6.0, 7.5, and 9.0 dS m-1) and two solution circulation frequencies (twice and thrice a day). In Experiment I, the evapotranspired depth was replaced using brackish water that was used to prepare each of the salinity levels (used exclusively), whereas in Experiment II, brackish water was used only to prepare each of the salinity levels and public water was used (electrical conductivity [ECw] = 0.12 dS m-1) for replacement in all treatments. The increase in the nutrient solution salinity reduced the production of DM and accumulation of nutrients; the reductions were more pronounced when brackish waters were used exclusively (Experiment I). However, the circulation of solutions thrice a day resulted in the harmful effects of the salinity effect. Replacing the evapotranspirated blade with water supply (Experiment II) mitigated the deleterious effects of salinity. Moreover, three circulations of the nutrient solution daily resulted in lower accumulation of inorganic Na+ and Cl- solutes and increased accumulation of nutrients N, P, K+, Ca2+, Mg2+, and S in the culture.

scholarly journals The quality properties, thiobarbituric acid (TBA) values and microstructure of chicken sausage with local red beetroot powder

Food Research ◽  
2021 ◽  
Vol 5 (S2) ◽  
pp. 113-119
Author(s):  
W. Swastike ◽  
E. Suryanto ◽  
Rusman ◽  
C. Hanim ◽  
Jamhari ◽  
...  
Keyword(s):  
Peroxide Value ◽  
Room Temperature ◽  
Thiobarbituric Acid ◽  
Quality Properties ◽  
Randomized Design ◽  
Powder Addition ◽  
Completely Randomized Design ◽  
Different Levels ◽  
Temperature Storage

This research was aimed to determine the quality properties, the microstructure of chicken sausage and Thiobarbituric acid (TBA) values with locally Indonesia red beetroot powder. The main ingredients of chicken sausage-making in this research were broiler chicken, filler, binder, beetroot powder, and spices. Red beetroot powder function as a filler was substituted tapioca starch in chicken sausage batter in three different levels. The combination of red beetroot powder with level 0, 1.0, 2.0 and 3.0% of total batter and shelf life at room temperature for 0, 1, 2 and 3 days. Each treatment consisted of five replications. The variables observed using quality properties (moisture, ash, fat, protein, crude fiber and calorie), microstructure and peroxide value of chicken sausage. The data of quality properties and peroxide value were analyzed by using one-way analysis (ANOVA) of Completely Randomized Design. The differences between means were analyzed by Duncan's New Multiple Ranges Test. The data of microstructure was analyzed by descriptive analyses. The moisture, protein, fat and ash contents for chicken sausages were significantly different (p<0.05). The chicken sausage with 2% substitution of beet powder produced chicken sausages with a high protein content of 14.77±0.02% while a low-fat content is 0.42±0.01%. Thiobarbituric acid (TBA) values of chicken sausages increased throughout the three days of room temperature storage (38°C). Chicken sausage formulated with red beetroot powder showed a significantly lower TBA value compared to the samples without red beetroot powder (p<0.05). In conclusion, a higher level of beetroot powder will improve the quality of chicken sausage and also the microstructure. The best level of beetroot powder addition was 2.0%. The addition of beetroot powder able to maintain fresh sausage conditions up to 2 days of storage at room temperature.

scholarly journals Physicochemical, Microbiological and Sensory Properties of Fermented Whey using Kombucha Inoculum

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Fitri Suciati ◽  
Nurliyani Nurliyani ◽  
Indratiningsih Indratiningsih
Keyword(s):  
Green Tea ◽  
Black Tea ◽  
Acetic Acid Bacteria ◽  
Solid Content ◽  
Sensory Properties ◽  
Whey Protein Concentrate ◽  
Plate Count ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Different Levels

This research aimed to determine physicochemical, microbiological and sensory properties of fermented whey using kombucha inoculum. The material used were kombucha, black tea, green tea, and whey protein concentrate. The research used factorial completely randomized design. The first factor was kombucha inoculum using different mediums (black and green tea) at different levels (5; 10; 15; dan 20%) as a second factor. The whey was fermented at 37oC for 39 hours. Research showed the use of kombucha inoculum using different medium (black and green tea) at different levels (5; 10; 15; dan 20%) did not affect (p>0.05) on microbiological properties (the number of Total Plate Count, total lactic acid bacteria, total acetic acid bacteria, and total yeast), dissolved protein content, lactose content, viscosity and acceptability. Black and green tea kombucha inoculum can be used in whey fermentation. Black tea kombucha fermented whey with 20% level addition has the best solid content. Relatively, kombucha fermented whey is quite acceptable.

scholarly journals Do the stage of plant and initial population of root knot nematode influence on sweet pepper development?

O Biológico ◽  
2020 ◽  
Vol 82 (1) ◽  
pp. 1-10
Author(s):  
Samara A. de Oliveira ◽  
Juliana M. O Rosa ◽  
Juliana Eulálio ◽  
Claudio Marcelo G. de Oliveira
Keyword(s):  
Sweet Pepper ◽  
Initial Population ◽  
Root Knot Nematode ◽  
One Pot ◽  
Leaf Pair ◽  
Final Population ◽  
Randomized Design ◽  
Meloidogyne Enterolobii ◽  
Shoot Weight ◽  
Reproduction Factor

The aim of this study was to investigate the response of three different stages (one, three and five leaf pairs) of sweet pepper (Capsicum annuum cv. Orazio) seedlings to five inoculation levels of Meloidogyne enterolobii (zero (control), 300, 1000, 3000 and 10000) under greenhouse conditions. Each plant was cultivated in one pot filled with 3.8 L of substrate. The test was a completely randomized design with four replications. The plants were assessed 60 days after inoculation, plant shoot weight, final population of nematodes, and reproduction factor were measured. The results were fitted to Seinhorst model: Y = m + (1-m). ZPi –T. The results showed a tolerance limit (T) of 2,500 nematodes for plants with one and three leaf pair, and 8,500 nematodes for the five-leaf pair plant.

scholarly journals RESISTANCE OF POTATO GENOTYPES TO Meloidogyne javanica

2017 ◽  
Vol 30 (2) ◽  
pp. 536-540 ◽  
Author(s):  
JAQUELINE TAVARES SCHAFER ◽  
CESAR BAUER GOMES ◽  
ARIONE DA SILVA PEREIRA ◽  
FERNANDA FERREIRA CRUZ ◽  
DANIELLE RIBEIRO DE BARROS
Keyword(s):  
Potato Cultivar ◽  
Meloidogyne Javanica ◽  
Temperate Climate ◽  
Initial Population ◽  
Nematode Reproduction ◽  
Potato Plants ◽  
Final Population ◽  
Susceptible Control ◽  
Different Levels ◽  
Reproduction Factor

ABSTRACT The objective of this study was to evaluate the resistance of a group of clones and cultivars from the potato breeding program of Embrapa to Meloidogyne javanica. The experiment was conducted at Embrapa Temperate Climate, Pelotas / RS, under greenhouse conditions at temperatures of 25 ± 5°C. Individual potato plants of different genotypes [BRSIPR Bel, BRS F63 (Camila), CL02-05, F23-11-06, F32-02-06, F38-03-07, F189-09-06, F23-24-06 and F22-01-08], kept in pots with sterilized soil were inoculated with 5,000 eggs and J2 of M. javanica, using six replicates per treatment. The susceptible control, potato cultivar BRS Ana was inoculated with the same level of inoculum. Fifty-five days after inoculation, the number of galls on the roots of each plant was determined as well as the number of protuberances caused by nematodes in the different treatments. Then, the roots of each plant were processed for counting the number of eggs and J2, as well as determining the nematode reproduction factor (FR: final population / initial population). All genotypes were susceptible (FR> 1.00) to M. javanica. However, there were different levels of susceptibility among the cultivars tested.

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