Validation and Application of a Methodology for Quantifying Levels of Parabens in Sports Supplements from Brazil Using Liquid Chromatography-Mass Spectrometry

Although parabens have been reported to be used as antimicrobial preservatives in personal care products, pharmaceuticals, and foodstuffs, little is known about the occurrence of these compounds in sports supplements. In this study, a simple, fast and sensitive liquid chromatography tandem mass spectrometry method for simultaneous determination of five commonly used parabens, methyl-, ethyl-, propyl-, butyl-, and benzylparaben in sports supplements was established. The analytical method was validated with the values of parameters being acceptable. The method proved reliable, accurate, and sensitive and was successfully applied for the quantitation of parabens in 85 sports supplements samples. Almost all sports supplements contained at least one of the parabens analyzed, and the total concentrations of five parabens ranged from below the limit of quantification to 145 μg g-1, with a median value of 0.171 μg g-1. Ethylparaben and methylparaben were the most frequent paraben analogs found in samples (detection rate > 60%). Propylparaben, butylparaben and benzylparaben are forbidden in food samples, their detection rates were 49.4, 21.2 and 4.7%, respectively. Therefore, our study suggests that Brazilian population may be exposed to estrogenic not allowed parabens and their harmful effects through the intake of sports food supplements.

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Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry

Toxins ◽

10.3390/toxins11040196 ◽

2019 ◽

Vol 11 (4) ◽

pp. 196 ◽

Cited By ~ 7

Author(s):

Ali Alsharif ◽

Yeun-Mun Choo ◽

Guan-Huat Tan

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Ochratoxin A ◽

Wheat Flour ◽

Food Samples ◽

Food Contaminants ◽

Relative Standard ◽

Liquid Chromatography Tandem Mass ◽

Food Matrices

Mycotoxins are common food contaminants which cause poisoning and severe health risks to humans and animals. The present study applied chemometric approach in liquid chromatography-tandem mass spectrometry (LC-MS/MS) optimization for simultaneous determination of mycotoxins, i.e., aflatoxins B1, B2, G1, and G2, and ochratoxin A. The validated quick, easy, cheap, effective, rugged, and safe (QuEChERS)-LC-MS/MS method was used to study the occurrence of mycotoxins in 120 food matrices. The recovery ranges from 81.94% to 101.67% with relative standard deviation (RSD) lesser than 11%. Through the developed method, aflatoxins were detected in raisin, pistachio, peanut, wheat flour, spice, and chili samples with concentration ranges from 0.45 to 16.93 µg/kg. Trace concentration of ochratoxin A was found in wheat flour and peanut samples which ranged from 1.2 to 3.53 µg/kg. Some of the tested food samples contained mycotoxins of above the European legal maximum limit.

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Simultaneous determination of steviol glycosides in food from stevia by liquid chromatography tandem mass spectrometry (LC-MS/MS)

Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam ◽

10.47866/2615-9252/vjfc.66 ◽

2019 ◽

Vol 2 (2) ◽

pp. 23-28

Author(s):

Huyen Trang Luu Thi ◽

Kim Oanh Vu Thi ◽

Trang Vu Thi ◽

Bien Nguyen Thi ◽

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...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Stevia Rebaudiana ◽

Food Samples ◽

Steviol Glycosides ◽

Tandem Mass ◽

Rebaudioside A ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass

The objective of this study is to develop a method for determination of steviol glycosides in food using liquid chromatography mass tandem spectrometry. The compounds were extracted from the matrices with methanol at 40°C within 60 minutes, and then determined by LC-MS/MS using C18 column (100 mm x 2.1 mm x 3.5 µm), the MS/MS detector with negative ESI mode. The calibration curves were linear in the range of 0.1 to 20 μg/mL; the RSD was of 1.74 – 5.01%; and the recovery was in range of 90 – 105%. The method was applied to analyze 20 food samples collected from markets in Hanoi (including dry stevia rebaudiana, stevia powder, stevia tea and soft drinks). The results showed that the composition of steviol glycosides was different from sample to sample. The most abundant steviol glycosides were stevioside and rebaudioside A.

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