The Protective Effect of Quercetin Against to Renal Tissue Damage Induced Carbontetrachloride in Rat

Abstract Purpose: Calcium dobesilate (CaD) has antioxidant and anti-inflammatory properties. In this study, the protective effects of CaD against hepatorenal damage induced by CCL4 in male mice were evaluated. Methods: Thirty male mice randomly were divided into five groups: Control, CaD 100 mg/kg, CCL4, CCL4+CaD 50 mg/kg, and CCL4+CaD 100 mg/kg. Drugs were administered orally once a day for 4-weeks. The liver and kidney indices (serum creatinine, blood urine nitrogen, alanine aminotransferase and aspartate aminotransferase levels) were determined. Also, hepatic and renal tissue oxidant/antioxidant markers (glutathione peroxidase, malondialdehyde, total antioxidant capacity, and superoxide dismutase) were measured. Cleaved caspase-3, Bax, and Bcl-2 protein levels were measured by immunoblotting method. The liver and kidney histopathological changes were evaluated by H&E staining.Results: CCL4 induces significant oxidative stress in the kidney and liver that was concomitant with functional and histopathological abnormalities in these organs in the CCL4 group versus the control (P<0.05). CaD could significantly improve the histopathological change in the liver and kidney tissues of CCL4+CaD 100 mg/kg mice versus the CCL4 group (P<0.05). In addition, CaD attenuated apoptosis in the liver and kidney tissues (P<0.05).Conclusion: The protective effect of CaD may be related to its anti-inflammatory and antioxidant properties.

Download Full-text

Thymoquinone Upregulates Catalase Gene Expression and Preserves the Structure of the Renal Cortex of Propylthiouracil-Induced Hypothyroid Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/3295831 ◽

2020 ◽

Vol 2020 ◽

pp. 1-15

Author(s):

Nasra Ayuob ◽

Maha Jameal Balgoon ◽

Ahmed A. El-Mansy ◽

Wafaa A. Mubarak ◽

Alaa El-Din L. Firgany

Keyword(s):

Gene Expression ◽

Body Weight ◽

Protective Effect ◽

Renal Cortex ◽

Nigella Sativa ◽

Serum Levels ◽

Renal Tissue ◽

Renal Diseases ◽

Catalase Gene ◽

Male Wistar Rats

Background. The association between hypothyroidism and renal diseases has been described in many studies. Nigella Sativa was among the recently reported natural product that has the potential to prevent renal tissue damage and fibrosis. The aim of this study was to evaluate the possible protective effect of thymoquinone on the structure of the renal cortex of hypothyroid rats and explore the mechanism behind it. Methods. An experimental model of hypothyroidism was induced in adult male Wistar rats by administration of propylthiouracil (6 mg/kg/body weight). One hypothyroid group was treated with thymoquinone at the dose of 50 mg/kg/body weight and compared to the untreated group. Thyroid function and oxidant/antioxidant status were assessed in the serum. Catalase gene expression was assessed using the real-time polymerase chain reaction. The kidney was assessed both histologically and immunohistochemically. Results. Administration of propylthiouracil resulted in a significant decrease in the serum levels of nitric oxide, reduced glutathione, and superoxide dismutase activity while the level of malondialdehyde significantly (p<0.001) increased. Administration of thymoquinone alleviated this effect on the thyroid hormones and significantly increased the serum levels of antioxidants. Thymoquinone significantly (p<0.001) upregulated catalase transcription by about 24-fold and could block the hypothyroidism-induced glomerular and tubular injury. Conclusion. Thymoquinone may have a potential protective effect against hypothyroidism-induced renal injury acting through the attenuation of the oxidative stress and upregulation of renal catalase gene expression.

Download Full-text

Protective effects of pentoxifylline on cigarette smoking-induced renal tissue damage in rats

Toxicology and Industrial Health ◽

10.1177/0748233710387006 ◽

2010 ◽

Vol 27 (4) ◽

pp. 335-340 ◽

Cited By ~ 1

Author(s):

Cuneyd Ozkurkcugil ◽

Melda Yardimoglu Yilmaz ◽

Levend Ozkan ◽

Sibel Kokturk ◽

Tonguc Isken

Keyword(s):

Cigarette Smoking ◽

Tissue Damage ◽

Renal Tissue ◽

Protective Effects

Download Full-text

Protective effect of chlorogenic acid on renal ischemia/reperfusion injury in rats

Archivio Italiano di Urologia e Andrologia ◽

10.4081/aiua.2020.2.153 ◽

2020 ◽

Vol 92 (2) ◽

Author(s):

Tuncay Toprak ◽

Cagri Akin Sekerci ◽

Hasan Riza Aydın ◽

Mehmet Akif Ramazanoglu ◽

Fatma Demet Arslan ◽

...

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Chlorogenic Acid ◽

Tissue Damage ◽

Sham Group ◽

Ischemia Reperfusion ◽

Statistical Significance ◽

Histological Evaluation ◽

Renal Histology ◽

Biochemical Examination

Objectives: Ischemia/reperfusion (I/R) injury is a common cause of renal injury and to date, many pharmacological agents have been identified to decrease I/R injury. One of the potential compound that can target I/R injury is chlorogenic acid (CGA). It has potent antiinflammatory, antibacterial, anti-oxidant, analgesic and antipyretic activities in in vitro experiments and in vivo animal models. The aim of the study was to investigate the protective characteristic of CGA on renal I/R injury. Material and Methods: 24 rats were randomly allocated to three groups (n = 8): Sham, I/R+CGA and I/R groups. CGA was administered intraperitoneally at a dose of 20 mg/kg, 10 min before reperfusion. I/R injury was achieved by clamping the left renal artery for 45 minutes, followed by reperfusion for 4 hours. The left kidneys of the rats were examined for tissue damage by histopathological and biochemical examination. For histological evaluation, EGTI scoring system was used. For biochemical examination total oxidant status, total antioxidant status and oxidative stress index were used. The power analysis indicated that 8 subjects per group would be required to produce 80% chance of achieving statistical significance at p < 0.05 level. The results are expressed as mean ± SD. Mann- Whitney U was performed for statistical analysis. Results: Histopathological examination of the tissue damage revealed that all kidneys in the sham group were normal. I-R group had significantly higher histopathological scores than other groups. Histopathological improvement was seen after CGA treatment. TAS, TOS and OSI values of I-R group were significantly higher than sham group (0.88 vs 0.76 (p: 0.004), 13.8 vs 7.04 (p: 0.021) and 0.15 vs 0.09 (p: 0.034), respectively). In CGA treated group TAS, TOS and OSI levels were 0.84, 6.47 and 0.07, respectively. CGA treatment resulted in significant improvement in TOS and OSI parameters. Conclusions: CGA treatment provided marked improvement in renal histology and suppressed oxidative stress. Thus, CGA may have a protective effect in renal tissue against I/R injury.

Download Full-text

Differential requirement for A2a and A3 adenosine receptors for the protective effect of inosine in vivo

Blood ◽

10.1182/blood-2002-11-3624 ◽

2003 ◽

Vol 102 (13) ◽

pp. 4472-4478 ◽

Cited By ~ 75

Author(s):

Gregorio Gomez ◽

Michail V. Sitkovsky

Keyword(s):

Protective Effect ◽

Adenosine Receptors ◽

Tissue Damage ◽

Wild Type ◽

In Vivo Models ◽

Inflammatory Conditions ◽

Severe Liver Damage ◽

Lung Tissue Damage ◽

Deficient Mice

AbstractInosine is an endogenous nucleoside with immunosuppressive properties that is known to inhibit the accumulation of proinflammatory cytokines and protect mice from endotoxin-induced inflammation and lung tissue damage. There are no known receptors specific for inosine, but A3 adenosine receptors (A3Rs) have been shown to bind inosine, resulting in mast cell degranulation and increased vascular permeability. The present study specifically addresses the requirement for A2aR and/or A3R for the protective effect of inosine in 2 experimental in vivo models of inflammatory disease. The data show that A3R is essential for protection against ConA-induced fulminant hepatitis since only A3R-expressing mice were protected by inosine whereas wild-type and A2aR-deficient mice exhibited severe liver damage even after administration of inosine. In addition, we show in a model of LPS-induced endotoxemia that inosine protected both A2aR-/- and A3R-/- mice from inflammation, but not A2aA3R double-null mice, indicating that in this model both A2aR and A3R were used by inosine. Thus, we demonstrate that A2a and A3 adenosine receptors are differentially utilized by inosine for the down-regulation of tissue damage under different inflammatory conditions in vivo. (Blood. 2003;102:4472-4478)

Download Full-text