scholarly journals Evaluation of the antimicrobial activity of purified Spodoptera littoralis hemolymph against some pathogenic bacteria

2021 ◽  
Vol 17 (1) ◽  
pp. 221-231
Author(s):  
Hanan Amer ◽  
Doaa Soliman ◽  
Wael Abdel-Mageed ◽  
Shimaa Mo’men ◽  
Tamer Roshdy ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Spodoptera Littoralis ◽  
Pathogenic Bacteria

ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Risa Nofiani ◽  
Siti Nurbetty ◽  
Ajuk Sapar
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Agar Medium ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Associated Bacteria ◽  
Minimum Inhibitory Concentrations ◽  
Sponge Associated Bacteria

<p>The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity.</p> <p>Keywords:Antimicrobial,Sponges associated bacteria,MICs</p>

Antimicrobial Activity of Phenolics and Glucosinolate Hydrolysis Products and their Synergy with Streptomycin against Pathogenic Bacteria

2010 ◽  
Vol 6 (3) ◽  
pp. 174-183 ◽  
Author(s):  
Maria Saavedra ◽  
Anabela Borges ◽  
Carla Dias ◽  
Alfredo Aires ◽  
Richard Bennett ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Hydrolysis Products

scholarly journals Antimicrobial activity of different F innish monofloral honeys against human pathogenic bacteria

Apmis ◽  
2012 ◽  
Vol 121 (9) ◽  
pp. 827-834 ◽  
Author(s):  
Sanna Huttunen ◽  
Kaisu Riihinen ◽  
Jussi Kauhanen ◽  
Carina Tikkanen‐Kaukanen
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Human Pathogenic Bacteria

scholarly journals Interrelations of Synthesis Method, Polyethylene Glycol Coating, Physico-Chemical Characteristics, and Antimicrobial Activity of Silver Nanoparticles

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2475
Author(s):  
Amirah Shafilla Mohamad Kasim ◽  
Arbakariya Bin Ariff ◽  
Rosfarizan Mohamad ◽  
Fadzlie Wong Faizal Wong
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Polyethylene Glycol ◽  
Pathogenic Bacteria ◽  
Synthesis Method ◽  
Chemical Characteristics ◽  
Synthesis Methods ◽  
Uniform Size ◽  
Transmission Electron Microscopy Analysis ◽  
Physico Chemical

Silver nanoparticles (AgNPs) have been found to have extensive biomedical and biological applications. They can be synthesised using chemical and biological methods, and coated by polymer to enhance their stability. Hence, the changes in the physico-chemical characteristics of AgNPs must be scrutinised due to their importance for biological activity. The UV-Visible absorption spectra of polyethylene glycol (PEG) -coated AgNPs displayed a distinctive narrow peak compared to uncoated AgNPs. In addition, High-Resolution Transmission Electron Microscopy analysis revealed that the shapes of all AgNPs, were predominantly spherical, triangular, and rod-shaped. Fourier-Transform Infrared Spectroscopy analysis further confirmed the role of PEG molecules in the reduction and stabilisation of the AgNPs. Moreover, dynamic light scattering analysis also revealed that the polydispersity index values of PEG-coated AgNPs were lower than the uncoated AgNPs, implying a more uniform size distribution. Furthermore, the uncoated and PEG-coated biologically synthesised AgNPs demonstrated antagonisms activities towards tested pathogenic bacteria, whereas no antagonism activity was detected for the chemically synthesised AgNPs. Overall, generalisation on the interrelations of synthesis methods, PEG coating, characteristics, and antimicrobial activity of AgNPs were established in this study.

Antimicrobial activity of phenolic acids against commensal, probiotic and pathogenic bacteria

2010 ◽  
Vol 161 (5) ◽  
pp. 372-382 ◽  
Author(s):  
Carolina Cueva ◽  
M. Victoria Moreno-Arribas ◽  
Pedro J. Martín-Álvarez ◽  
Gerald Bills ◽  
M. Francisca Vicente ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Acids ◽  
Pathogenic Bacteria

scholarly journals In Silico Analyses of Rice Thionin Genes and the Antimicrobial Activity of OsTHION15 Against Phytopathogens

2019 ◽  
Vol 109 (1) ◽  
pp. 27-35
Author(s):  
Krissana Boonpa ◽  
Suparuk Tantong ◽  
Kamonwan Weerawanich ◽  
Pawinee Panpetch ◽  
Onanong Pringsulaka ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Stress Responses ◽  
In Silico ◽  
Pathogenic Bacteria ◽  
Hyphal Growth ◽  
In Planta ◽  
Recombinant Peptide ◽  
Plant Disease Control ◽  
Helminthosporium Oryzae ◽  
In Silico Analyses

Thionins are a family of antimicrobial peptides. We performed in silico expression analyses of the 44 rice (Oryza sativa) thionins (OsTHIONs). Modulated expression levels of OsTHIONs under different treatments suggest their involvement in many processes, including biotic, abiotic, and nutritional stress responses, and in hormone signaling. OsTHION15 (LOC_Os06g32600) was selected for further characterization based on several in silico analyses. OsTHION15 in O. sativa subsp. indica ‘KDML 105’ was expressed in all of the tissues and organs examined, including germinating seed, leaves, and roots of seedlings and mature plants, and inflorescences. To investigate the antimicrobial activity of OsTHION15, we produced a recombinant peptide in Escherichia coli Rosetta-gami (DE3). The recombinant OsTHION15 exhibited inhibitory activities toward rice-pathogenic bacteria such as Xanthomonas oryzae pv. oryzae and Pectobacterium carotovorum pv. atroseptica, with minimum inhibitory concentrations of 112.6 and 14.1 µg ml−1, respectively. A significant hyphal growth inhibition was also observed toward Fusarium oxysporum f. sp. cubense and Helminthosporium oryzae. In addition, we demonstrated the in planta antibacterial activity of this peptide in Nicotiana benthamiana against X. campestris pv. glycines. These activities suggest the possible application of OsTHION15 in plant disease control.

scholarly journals Algae-meditated route to cuprous oxide (Cu2O) nanoparticle: differential expression profile of MALAT1 and GAS5 LncRNAs and cytotoxic effect in human breast cancer

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Parisa Taherzadeh-Soureshjani ◽  
Mohammad Chehelgerdi
Keyword(s):  
Breast Cancer ◽  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Normal Cell ◽  
Pathogenic Bacteria ◽  
Control Cell ◽  
Normal Cell Line ◽  
Control Cell Line

Abstract Background Breast cancer (BC), as the most widely recognized disease in women worldwide, represents about 30% of all cancers impacting women. This study was aimed to synthesize Cu2O nanoparticles from the cystoseira myrica algae (CM-Cu2O NPs) assess their antimicrobial activity against pathogenic bacteria and fungi. We evaluated the expression levels of lncRNAs (MALAT1 and GAS5) and apoptosis genes (p53, p27, bax, bcl2 and caspase3), their prognostic roles. Methods In this study, CM-Cu2O NPs synthesized by cystoseira myrica algae extraction used to evaluate its cytotoxicity and apoptotic properties on MDA-MB-231, SKBR3 and T-47D BC cell lines compared to HDF control cell line. The CM-Cu2O NPs was characterized by UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM). The antimicrobial activity of CM-Cu2O NPs was assessed against pathogenic bacteria, staphylococcus aureus (S. aureus) PTCC 1112 bacteria as a standard gram-positive bacteria and pseudomonas aeruginosa (P. aeruginosa) PTCC 1310 as a standard gram-negative bacterium. Expression profile of MALAT1 and GAS5 lncRNAs and apoptosis genes, i.e., p27, bax, bcl2 and caspase3 genes, were calculated utilizing qRT-PCR. The changes in the expression levels were determined using the DDCT method. Results MALAT1 was upregulated in MDA-MB-231, SKBR3 and T-47D BC (p < 0.01), while GAS5 was downregulated in SKBR3 and T-47D cell lines tested compared with HDF control cell line (p < 0.05) was found. The results revealed that, p27, bax and caspase3 were significantly upregulated in BC cell lines as compared with normal cell line. Bcl2 expression was also significantly increased in MDA-MB-231 and T47D cell lines compared with normal cell line, but bcl2 levels were downregulated in SKBR3 cell line. Conclusions Our results confirm the beneficial cytotoxic effects of green-synthesized CM-Cu2O NPs on BC cell lines. This nanoparticle decreased angiogenesis and induces apoptosis, so we conclude that CM-Cu2O NPs can be used as a supplemental drug in cancer treatments. Significantly, elevated circulating lncRNAs were demonstrated to be BC specific and could differentiate BC cell lines from the normal cell lines. It was demonstrated that lncRNAs used in this study and their expression profiles can be created as biomarkers for early diagnosis and prognosis of BC. Further studies utilizing patients would give recognizable identification of lncRNAs as key players in intercellular interactions.

Isolation and antimicrobial activity of Streptomyces tubercidicus against pathogenic bacteria and fungi

2010 ◽  
Author(s):  
R. Priscila Ratti ◽  
A.C.M. Toledo Piza ◽  
A.C. Granato ◽  
C.O. Hokka ◽  
C. Paiva de Sousa
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Bacteria And Fungi

Phytochemical composition and in vitro screening of the antimicrobial activity of essential oils on oral pathogenic bacteria

2017 ◽  
Vol 32 (5) ◽  
pp. 544-551 ◽  
Author(s):  
Roberta Tardugno ◽  
Federica Pellati ◽  
Ramona Iseppi ◽  
Moreno Bondi ◽  
Giacomo Bruzzesi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
In Vitro Screening ◽  
Phytochemical Composition
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