scholarly journals Interrelations of Synthesis Method, Polyethylene Glycol Coating, Physico-Chemical Characteristics, and Antimicrobial Activity of Silver Nanoparticles

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2475
Author(s):  
Amirah Shafilla Mohamad Kasim ◽  
Arbakariya Bin Ariff ◽  
Rosfarizan Mohamad ◽  
Fadzlie Wong Faizal Wong
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Polyethylene Glycol ◽  
Pathogenic Bacteria ◽  
Synthesis Method ◽  
Chemical Characteristics ◽  
Synthesis Methods ◽  
Uniform Size ◽  
Transmission Electron Microscopy Analysis ◽  
Physico Chemical

Silver nanoparticles (AgNPs) have been found to have extensive biomedical and biological applications. They can be synthesised using chemical and biological methods, and coated by polymer to enhance their stability. Hence, the changes in the physico-chemical characteristics of AgNPs must be scrutinised due to their importance for biological activity. The UV-Visible absorption spectra of polyethylene glycol (PEG) -coated AgNPs displayed a distinctive narrow peak compared to uncoated AgNPs. In addition, High-Resolution Transmission Electron Microscopy analysis revealed that the shapes of all AgNPs, were predominantly spherical, triangular, and rod-shaped. Fourier-Transform Infrared Spectroscopy analysis further confirmed the role of PEG molecules in the reduction and stabilisation of the AgNPs. Moreover, dynamic light scattering analysis also revealed that the polydispersity index values of PEG-coated AgNPs were lower than the uncoated AgNPs, implying a more uniform size distribution. Furthermore, the uncoated and PEG-coated biologically synthesised AgNPs demonstrated antagonisms activities towards tested pathogenic bacteria, whereas no antagonism activity was detected for the chemically synthesised AgNPs. Overall, generalisation on the interrelations of synthesis methods, PEG coating, characteristics, and antimicrobial activity of AgNPs were established in this study.

Biosynthesis of Silver Nanoparticles by Punica Granatum Peel Extract and their Biological Activity on different Pathogenic Bacteria

2021 ◽  
Vol 19 (9) ◽  
pp. 38-45
Author(s):  
Hussein H. Al-Turnachy ◽  
Fadhilk. alibraheemi ◽  
Ahmed Abd Alreda Madhloom ◽  
Zahraa Yosif Motaweq ◽  
Nibras Yahya Abdulla
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Biological Activity ◽  
Pathogenic Bacteria ◽  
Punica Granatum ◽  
Gram Positive ◽  
Gram Negative ◽  
Inhibition Zones ◽  
Peel Extract

The present study was included the assessment of the antimicrobial activity of AgNPs synthesized by Punica granatum peel extract against pathogenic bacteria by testing warm aqueous P. granatum peel extract and silver nanoparticles. Punica granatum indicated potency for AgNP extracellular nanobiosynthesis after addition of silver nitrate (AgNO3) 4mM to the extract supernatant, in both concentrations (100mg and 50mg). The biogenic AgNPs showed potency to inhibit both gram-negative and gram-positive bacterial growth. Zons of inhibition in (mm) was lesser in gram-positive than gram-negative bacteria. The resulted phytogenic AgNPs gave higher biological activity than warm aqueous Punica granatum peel extract. The inhibition zone of the phytogenic AgNPs on E. coli reached 17.53, 22.35, and 26.06 mm at (0.1, 0.5, and 1) mg/ml respectively. While inhibition zones of Punica warm aqueous extract reached 5.33, 10.63, and 16.08 mm at the same concentrations. phytogenic AgNPs gave smaller inhibition zones in gram-positive than gram- negative. Cytotoxic activity of the phytogenic AgNPs was assayed in vitro agaist human blood erythrocytes (RBCs), spectroscopic results showed absorbance at 540 nm hemolysis was observed. In general, AgNPs showed least RBCs hemolysis percentage, at 1 mg/ml concentration, hemolysis percentage was (4.50%). This study, concluded that the Punica granatum peel extract has the power of synthses of AgNPs characterized by broad spectrum antimicrobial activity with cyto-toxicity proportional to AgNPs concentration.

scholarly journals Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1936 ◽  
Author(s):  
M. A. Abu-Saied ◽  
Mohamed Elnouby ◽  
Tarek Taha ◽  
Muhammad El-shafeey ◽  
Ali G. Alshehri ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Drinking Water ◽  
Pathogenic Bacteria ◽  
Polymeric Nanoparticles ◽  
Wide Distribution ◽  
Spectrophotometric Analysis ◽  
Transmission Electron ◽  
Green Bottle

The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water. The current applied treatments face some limitations. In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast. The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles. The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30–100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer. The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength. The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens was highly effective. These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.

scholarly journals Biosynthetic potential assessment of four food pathogenic bacteria in hydrothermally silver nanoparticles fabrication

2019 ◽  
Vol 8 (1) ◽  
pp. 629-634 ◽  
Author(s):  
Amir Rahimirad ◽  
Afshin Javadi ◽  
Hamid Mirzaei ◽  
Navideh Anarjan ◽  
Hoda Jafarizadeh-Malmiri
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Pathogenic Bacteria ◽  
Heating Time ◽  
Synthesis Process ◽  
Ag Nps ◽  
Physico Chemical ◽  
Minimum Particle Size ◽  
Particle Size Analyzer

Abstract Silver nanoparticles (Ag NPs) were synthesized using four pathogenic bacterial extracts namely, Bacillus cereus, E. coli, Staphylococcus aureus and Salmonella entericasubsp.enterica. Synthesis process were hydrothermally accelerated using temperature, pressure and heating time of 121°C, 1.5 bar ad 15 min. Physico- chemical characteristics of the fabricated Ag NPs, including, particle size, polydispersity index (PDI), zeta potential, broad emission peak (λmax) and concentration were evaluated using UV-Vis spectrophotometer and dynamic light scattering (DLS) particle size analyzer. Furthermore, main existed functional groups in the provided bacterial extracts were recognized using Fourier transform infrared spectroscopy. The obtained results revealed that two main peaks were detected around 3453 and 1636.5 cm-1, for all bacterial extracts, were interrelated to the stretching vibrations of hydroxyl and amide groups which those had key roles in the reduction of ions and stabilizing of the formed Ag NPs. The results also indicated that, Ag NPs with much desirable characteristics, including minimum particle size (25.62 nm) and PDI (0.381), and maximum zeta potential (-29.5 mV) were synthesized using S. e. subsp. enterica extract. λmax, absorbance and concentration values for the fabricated Ag NPs with this bacterial extract were 400 nm, 0.202% a.u. and 5.87 ppm.

scholarly journals Characterization and Biocompatibility Properties of Silver Nanoparticles Produced Using Short Chain Polyethylene Glycol

2010 ◽  
Vol 10 ◽  
pp. 29-37 ◽  
Author(s):  
Maryam Jokar ◽  
Russly Abdul Rahman ◽  
Nor Azowa Ibrahim ◽  
Luqman Chuah Abdullah ◽  
Tan Chin Ping
Keyword(s):  
Silver Nanoparticles ◽  
Polyethylene Glycol ◽  
Chemical Reduction ◽  
Radical Scavenging ◽  
Agar Plate ◽  
Antimicrobial Properties ◽  
Silver Ions ◽  
Colloidal Dispersion ◽  
Short Chain ◽  
Uniform Size

Silver nanoparticles are of interest due to their unique physicochemical and antimicrobial properties. The nanoparticles were produced by chemical reduction using short chain polyethylene glycol (PEG) as reducing agent, solvent and stabilizer in absence of other chemicals. Silver nanoparticles were separated from colloidal dispersion by ultra centrifuge at 14000 rpm. The reduction of silver ion (Ag+) to silver nanoparticles (Agº) was monitored by pH measurement and UV-visible spectroscopy of colloidal dispersion at fixed intervals. Silver nanoparticles were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM) and diphenyl-picrylhydrazyl (DPPH) radical scavenging method. Antimicrobial activity of silver nanoparticles was investigated against Escherichia coli, Staphylococcus aureus and Vibrio parahaemolyticus by agar plate test. Results indicated 51.5% conversion efficiency of silver ions to silver nanoparticles. Colloidal dispersion containing 4.12 mg/ml silver nanoparticles showed uniform size of 5.5 ± 1.1 nm with a typical visible spectra band at 447 nm. Silver nanoparticles showed significant (p < 0.05) antimicrobial efficiency and with concentration of 100 ppm resulted in 46.22%, 66.51% and 69.06% inhibition against S. aureus, E. coli and V. parahaemolyticus, respectively. The nanoparticles were also found to reduce DPPH free radical up to 88.9%. Results of this study proved that the silver nanoparticles produced by polyethylene glycol possess antimicrobial and antioxidant activity.

scholarly journals Natural synthesis of silver nanoparticles from grape fruit juice and estimation of antimicrobial activity

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Mehreen Fatima ◽  
Azra Quraishi ◽  
Maida Irfan
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Synthesis Method ◽  
Antimicrobial Effect ◽  
Tomato Juice ◽  
Zone Of Inhibition ◽  
Nanoparticle Preparation ◽  
Biochemical Research ◽  
Grape Fruit Juice

Silver has been known for its antimicrobial activity for a very long time. Formulation of silver particles that range from 1-100nm in size makes it even more potent to induce antimicrobial effect. Green chemistry has started to become more frequent in the field of biochemical research. Silver nanoparticles synthesized from green synthesis method provide a cheap and environmental friendly method of nanoparticle preparation. The aim of the current study is green synthesis of silver nanoparticles using tomato juice as reducing and capping agent and evaluation of its antimicrobial activity. The stability and conformation of SNPs was determined by UV-visible spectroscopy. The antimicrobial activity of synthesized SNPs was determined against E.coli DH5α. Ultraviolet spectroscopic analysis offered peak at 400 nm that indicate the production of SNPs of adequate size.  E.coli DH5α showed considerable decrease upon introduction of SNPs to the bacterial inoculum. Upon increasing the concentration of silver nanoparticles an increase in zone of inhibition was recorded.  For 70µg/ml of SNPs, the zone of inhibition was 0.5 cm, while 0.6 cm, 0.7 cm and 0.7cm was recorded for 100µg/ml, 150µg/ml and 200µg/ml of SNPs respectively. The efficacy of antimicrobial activity of SNPs derived from tomato juice proves its potential use in pharmaceutical and medicinal industries for synthesis of nanomedicine.  

scholarly journals SEMI-INDUSTRIAL TECHNOLOGY FOR SYNTHESIS OF TETRACYANOETHYLENE

2020 ◽  
Vol 63 (6) ◽  
pp. 72-79
Author(s):  
Konstantin V. Lipin ◽  
Sergey V. Fedoseev
Keyword(s):  
Carbon Disulfide ◽  
Raw Materials ◽  
Synthesis Method ◽  
Sodium Cyanide ◽  
Reaction Mass ◽  
Technological Scheme ◽  
Chemical Characteristics ◽  
Auxiliary Equipment ◽  
Physico Chemical ◽  
Selection Of

The goal of our work is to develop a synthesis of tetracyanoethylene suitable for scaling and to design a technological scheme of the process based on it. To achieve this goal, a tetracyanoethylene synthesis method was originally developed, which consists of the following stages: obtaining sodium cyanodithioformate based on carbon disulfide and sodium cyanide using N, N-dimethylformamide as a solvent; obtaining tetracyano-1,4-dithiine by treating sodium cyanodithioformate with chlorine; obtaining tetracyanoethylene from tetracyano-1,4-dithiine by sequential interaction with sodium cyanide and chlorine. The developed method was tested in the laboratory, where it showed its suitability. Therefore, further on the base of this method, a technological scheme of the process was designed. A description of the scheme and the necessary sequence of operations are compiled. The interaction takes place in the reactors R-1, R-2, R-3. Pumps N-1–4 provided the pumping the reaction mass. Also, in the technological scheme there is an F-1 Nutsche filter for separation of the suspension. To isolate and purify the obtained tetracyanoethylene, an I-1 vacuum evaporator and a SA-1 sublimation apparatus are used. The necessary equipment was selected for the technological scheme: Р-1 reactor made of Teflon with a volume of 200 l with a stirrer and a jacket; two reactors Р-2 and Р-3 made of glass with a volume of 200 l with a stirrer and a jacket; Ф-1 Nutsche filter made of thick-walled polypropylene pipes; sublimation apparatus CA-1 continuous. The selection of the main and auxiliary equipment was carried out on the base of the chemical and physico-chemical characteristics of the reagents, technological conditions and the characteristics of the process. This method of obtaining compares favorably with the use of simple and cheap raw materials common in the chemical industry and the use of standard processing equipment.

scholarly journals Green synthesis of silver nanoparticles using Calotropis gigantea and its characterization using UV-Vis Spectroscopy

2022 ◽  
Vol 951 (1) ◽  
pp. 012090
Author(s):  
P Kemala ◽  
R Idroes ◽  
K Khairan ◽  
T E Tallei ◽  
M Ramli ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Synthesis Method ◽  
Colour Change ◽  
Synthesis Methods ◽  
Phytochemical Analysis ◽  
Geothermal Area ◽  
Calotropis Gigantea ◽  
Yellow Solution ◽  
Vis Spectroscopy

Abstract The use of Calotropis gigantea from Ie Seu-Um, Aceh Besar geothermal area for silver nanoparticles (AgNPs) synthesized has been reported. The process of synthesis AgNPs can be carried out with chemical methods, physical methods, and green synthesis methods, but in this study, the process is focused on the green synthesis method using C. gigantea flowers and leaves extract from Ie Seu-Um geothermal area, Aceh Besar. Phytochemical analysis showed that C. gigantea leaves contain alkaloids, steroids, tannins, phenolics, and saponins, while the flowers of C. gigantea contain alkaloids, terpenoids, steroids, flavonoids, tannins, phenolics, and saponins. The reaction of the AgNPs formation was observed by colour change formed. AgNPs-C. gigantea flower extract dan AgNPs-C. gigantea leaves extract showed the reddish-brown and brownish-yellow solution respectively after 48 h incubation in dark condition at room temperature. The result of the reaction characterized using UV-Vis Spectrophotometry showed that the phenomenon of Surface plasmone resonance (SPR) occurs in the mixture of nanoparticles formed. The concentration of AgNO3 as a reagent affected the SPR phenomenon. The result showed that particles formed are the same size and shape.

scholarly journals Comparative analysis of different methods for graphene nanoribbon synthesis

2013 ◽  
Vol 67 (1) ◽  
pp. 147-156
Author(s):  
Dragana Tosic ◽  
Zoran Markovic ◽  
Svetlana Jovanovic ◽  
Momir Milosavljevic ◽  
Biljana Todorovic-Markovic
Keyword(s):  
Comparative Analysis ◽  
Gamma Irradiation ◽  
Carbon Nanomaterials ◽  
Graphene Nanoribbons ◽  
Synthesis Method ◽  
Chemical Vapour ◽  
Morphological Properties ◽  
Synthesis Methods ◽  
Uniform Size ◽  
Atomic Force

Graphene nanoribbons (GNRs) are thin strips of graphene that have captured the interest of scientists due to their unique structure and promising applications in electronics. This paper presents the results of a comparative analysis of morphological properties of graphene nanoribbons synthesized by different methods. Various methods have been reported for graphene nanoribons synthesis. Lithography methods usually include electron-beam (e-beam) lithography, atomic force microscopy (AFM) lithography, and scanning tunnelling microscopy (STM) lithography. Sonochemical and chemical methods exist as well, namely chemical vapour deposition (CVD) and anisotropic etching. Graphene nanoribbons can also be fabricated from unzipping carbon nanotubes (CNTs). We propose a new highly efficient method for graphene nanoribbons production by gamma irradiation of graphene dispersed in cyclopentanone (CPO). Surface morphology of graphene nanoribbons was visualized with atomic force and transmission electron microscopy. It was determined that dimensions of graphene nanoribbons are inversely proportional to applied gamma irradiation dose. It was established that the narrowest nanoribbons were 10-20 nm wide and 1 nm high with regular and smooth edges. In comparison to other synthesis methods, dimensions of graphene nanoribbons synthesized by gamma irradiation are slightly larger, but the yield of nanoribbons is much higher. Fourier transform infrared spectroscopy was used for structural analysis of graphene nanoribbons. Results of photoluminescence spectroscopy revealed for the first time that synthesized nanoribbons showed photoluminescence in the blue region of visible light in contrast to graphene nanoribbons synthesized by other methods. Based on disclosed facts, we believe that our synthesis method has good prospects for potential future mass production of graphene nanoribbons with uniform size, as well as for future investigations of carbon nanomaterials for applications in optoelectronics and biological labeling.

scholarly journals Biosynthesis, characterization, and in vitro assessment on cytotoxicity of actinomycete-synthesized silver nanoparticles on Allium cepa root tip cells

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Sreenivasa Nayaka ◽  
Bidhayak Chakraborty ◽  
Meghashyama Prabhakara Bhat ◽  
Shashiraj Kareyallappa Nagaraja ◽  
Dattatraya Airodagi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Allium Cepa ◽  
Pathogenic Bacteria ◽  
Synthesis Method ◽  
In Vitro Cytotoxicity ◽  
Root Tip ◽  
Average Size ◽  
Tip Cells ◽  
Root Tip Cells

Abstract Background The industrial production of silver nanoparticles (AgNPs) and its commercial applications are being considerably increased in recent times, resulting in the release of AgNPs in the environment and enhanced probability of contaminations and their adverse effects on living systems. Based on this, the present study was conducted to evaluate the in vitro cytotoxicity of actinomycete-synthesized AgNPs on Allium cepa (A. cepa) root tip cells. A green synthesis method was employed for biosynthesis of AgNPs from Streptomyces sp. NS-33. However, morphological, physiological, biochemical, and molecular analysis were carried out to characterize the strain NS-33. Later, the synthesized AgNPs were characterized and antibacterial activity was also carried out against pathogenic bacteria. Finally, cytotoxic activity was evaluated on A. cepa root tip cells. Results Results showed the synthesis of spherical and polydispersed AgNPs with a characteristic UV-visible (UV-Vis.) spectral peak at 397 nm and average size was 32.40 nm. Energy dispersive spectroscopy (EDS) depicted the presence of silver, whereas Fourier transform infrared (FTIR) studies indicated the presence of various functional groups. The phylogenetic relatedness of Streptomyces sp. NS-33 was found with Streptomyces luteosporeus through gene sequencing. A good antibacterial potential of AgNPs was observed against two pathogenic bacteria. Concerning cytotoxicity, a gradually decreased mitotic index (MI) and increased chromosomal aberrations were observed along with the successive increase of AgNPs concentration. Conclusions Therefore, the release of AgNPs into the environment must be prevented, so that it cannot harm plants and other beneficial microorganisms.

scholarly journals SILVER NANOPARTICLES FROM TRIANTHEMA PORTULACASTRUM: GREEN SYNTHESIS, CHARACTERIZATION, ANTIBACTERIAL AND ANTICANCER PROPERTIES

2017 ◽  
Vol 10 (3) ◽  
pp. 308
Author(s):  
Gowri Shankar Krishnan ◽  
Namrata Pradhan ◽  
Masilamani K ◽  
Albin T Fleming
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Pathogenic Bacteria ◽  
Synthesis Method ◽  
Silver Ions ◽  
Bacterial Strains ◽  
X Ray ◽  
Nih 3T3 ◽  
Dose Dependent

ABSTRACTObjective: In this study, silver nanoparticles (SNPs) were synthesized using an aqueous extract of Trainthema portulacastrum and silver ions (Ag+)which have been proven against certain pathogenic bacterial strains and hepatocellular carcinoma (HepG2) cell line.Methods: The bio fabricated nanoparticles were confirmed by surface plasmon resonance which were characterized by biophysical measuresutilizing the ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray, and transmission electron microscope(TEM), Fourier transform infrared spectroscopy, particle size analyzer, and X-ray diffraction. Antibacterial efficacy against Enterobacter aerogens,Proteus mirabilis, Escherichia coli, Staphylococcus epidermis, and Bacillus subtilis. The effect of SNPs tested against HepG2 and NIH/3T3 cell lineexhibits a dose-dependent toxicity.Results and Conclusion: The SEM and TEM images confirmed the presence of spherical and hexagonal shape (0.3-4 μm) of nanocrystalline particleswith the size range of 11.5-29.2 nm. The average particles size of SNPs is 190.3±17.0 nm. Antibacterial activity was carried out by agar well diffusionmethod against different pathogenic bacteria of which B. subtilis showed a significant zone of inhibition 8.66 mm and 12.0 mm for aqueous plantextract and synthesized SNPs. The effect of SNPs tested against HepG2 and NIH/3T3 cell line exhibits a dose-dependent toxicity. In case of HepG2, thecell viability was decreased to 50% (IC50) at the concentration of 173.8±0.84 μg/mL. From the results, it can be concluded that the SNPs fabricatedusing green synthesis method will be a promising candidate in the biomedical field, due to its high bioactive properties.Keywords: Silver nanoparticles, Trainthema portulacastrum, Antibacterial activity, Cytotoxic activity.

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