scholarly journals Synergistic physic-chemical pretreatment of lignocellulosic sugarcane bagasse via freezing and alkaline processes

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Keyword(s):  
Sugarcane Bagasse ◽  
Chemical Pretreatment

scholarly journals Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vihang S. Thite ◽  
Anuradha S. Nerurkar
Keyword(s):  
Cell Wall ◽  
Sugarcane Bagasse ◽  
Structural Changes ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Gravimetric Analysis ◽  
Chemical Pretreatment ◽  
Cell Wall Degrading Enzymes ◽  
First Time ◽  
Soluble Components

Abstract After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.

scholarly journals Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Rozina Rashid ◽  
Uroosa Ejaz ◽  
Firdous Imran Ali ◽  
Imran Ali Hashmi ◽  
Ahmed Bari ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Sugarcane Bagasse ◽  
Fermentation Medium ◽  
Chemical Pretreatment ◽  
Chemical Methods ◽  
Xylanase Production ◽  
Box Behnken Design ◽  
Combined Pretreatment ◽  
Scanning Electron ◽  
Better Than

Abstract Background Lignin in sugarcane bagasse (SB) hinders its utilization by microorganism, therefore, pretreatment methods are employed to make fermentable components accessible to the microbes. Multivariate analysis of different chemical pretreatment methods can aid to select the most appropriate strategy to valorize a particular biomass. Results Amongst methods tested, the pretreatment by using sodium hydroxide in combination with methyltrioctylammonium chloride, an ionic liquid, (NaOH+IL) was the most significant for xylanase production by Bacillus aestuarii UE25. Investigation of optimal levels of five significant variables by adopting Box-Behnken design (BBD) predicted 20 IU mL− 1 of xylanase and experimentally, a titer of 17.77 IU mL− 1 was obtained which indicated the validity of the model. The production kinetics showed that volumetric productivity of xylanase was much higher after 24 h (833.33 IU L− 1 h− 1) than after 48 h (567.08 IU L− 1 h− 1). The extracted xylan from SB induced more xylanase in the fermentation medium than pretreated SB or commercially purified xylan. Nuclear Magnetic Resonance, Fourier transform infrared spectroscopy and scanning electron microscopy of SB indicated removal of lignin and changes in the structure of SB after NaOH+IL pretreatment and fermentation. Conclusion Combined pretreatment of SB with alkali and methyltrioctylammonium chloride appeared better than other chemical methods for bacterial xylanase production and for the extraction of xylan form SB.

scholarly journals PHYSICAL AND CHEMICAL PRETREATMENT OF SUGARCANE BAGASSE FOR ENHANCED ACID HYDROLYSIS

2020 ◽  
Vol 54 (7-8) ◽  
pp. 699-704
Author(s):  
JANAÍNA ALVES CARVALHO ◽  
◽  
MATHEUS FELIPE FREIRE PEGO ◽  
PAULO RICARDO GHERARDI HEIN ◽  
WILLIAN MIGUEL DA SILVA BORGES ◽  
...  
Keyword(s):  
Acid Hydrolysis ◽  
Sugarcane Bagasse ◽  
Chemical Pretreatment ◽  
Physical And Chemical

scholarly journals Tamarind extract pretreatment: Valorization of sugarcane bagasse for cellulase production by Aspergillus flavus

2021 ◽  
Vol 23 (2) ◽  
pp. 25-34
Author(s):  
M. O. Kazeem ◽  
K. A. Ajijolakewu ◽  
A.M. Ahmed El - Imam ◽  
R. B. Salau
Keyword(s):  
Weight Loss ◽  
Aspergillus Flavus ◽  
Sugarcane Bagasse ◽  
Colour Change ◽  
Cellulase Production ◽  
Reducing Sugar ◽  
Chemical Pretreatment ◽  
Highest Weight ◽  
Filter Paper Assay ◽  
Carboxy Methyl

Effective pretreatment is crucial for cellulase production from sugarcane bagasse. Pretreatment with tamarind extract could reduce the hazardous effect associated with chemical pretreatment. The present work investigated tamarind (Tamarindus indica) extract in combination with H2SO4 and thermal pretreatment of sugarcane bagasse for cellulase production by Aspergillus flavus. The sugarcane bagasse was pretreated with tamarind extract pH 2 and pH 4, followed by 1% H2SO4 and thermal treatment at 121°C for 15 min. The pretreatment slurry was analysed for reducing sugar while solid bagasse was analysed for weight loss. Aspergillus flavus grew on sugarcane bagasse under solid state fermentation and the Carboxy Methyl Cellulase (CMCase) and Filter Paper Assay (FPA) activities were compared on the various pretreatments. The pretreatments changed the visible morphology of the sugarcane bagasse observed by the swelling, fibrous appearance and colour change. Pretreatment slurry yielded highest soluble reducing sugar at 60.01 mg/ml in tamarind extract (pH 4/1% H2SO4 ) and highest weight  loss of solids at 73.70% in tamarind extract (pH 2/1% H2SO4 /thermal 121°C). Aspergillus flavus performed better on tamarind extract (pH 2/1% H2SO4 ) by producing optimal CMCase and FPA activities at 0.100 U/ml and 0.409 U/ml respectively after 3 days of fermentation. Cellulase was maximally active at temperature of 50 °C. The tamarind extract pretreatment successfully proved to be an alternative organo-chemical pretreatment of sugarcane bagasse as evidenced by the physical properties, soluble reducing sugars and cellulase activities. Keywords: Aspergillus flavus, Cellulase, Pretreatment, Sugarcane bagasse, Tamarind extract

Effect of Chemical Pretreatment of Sugarcane Bagasse on Biogas Production

2020 ◽  
Vol 21 ◽  
pp. 1937-1942 ◽  
Author(s):  
Maninder Kaur ◽  
Neetu ◽  
Yajvender Pal Verma ◽  
Sanchita Chauhan
Keyword(s):  
Sugarcane Bagasse ◽  
Biogas Production ◽  
Chemical Pretreatment

Catalytic Hydrolysis of Pretreated Sugarcane Bagasse over Acid-Activated Vermiculite Catalysts

2015 ◽  
Vol 365 ◽  
pp. 212-218
Author(s):  
B.R.P. Nunes ◽  
Líbia de Sousa Conrado ◽  
C.R.S. Morais
Keyword(s):  
Aqueous Solution ◽  
Sugarcane Bagasse ◽  
High Performance ◽  
Lignin Content ◽  
Catalytic Hydrolysis ◽  
Chemical Pretreatment ◽  
Before And After ◽  
Pretreated Sugarcane Bagasse ◽  
Hydrolysis Of ◽  
Vermiculite Clay

Aiming at obtaining glucose, we studied the chemical pretreatment (NaOH + H2SO4) and the hydrolysis of sugarcane bagasse using as catalyst the acid-treated vermiculite clay. Samples of the bagasse before and after the treatment were characterized as to the fiber content and XRD. It has been found that the chemical pretreatment showed satisfactory results providing a decrease of 40% in the lignin content and of 43% in the hemicellulose content, regarding to the bagasse in natura. Catalytic tests in aqueous solution were performed at 200°C, to evaluate the use of vermiculite treated as a catalyst for the hydrolysis of sugarcane bagasse. The reaction product was filtered and the supernatant was analyzed by high performance liquid chromatography. A yield of 6.18% in glucose was achieved.

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