Isolation, Characterization and Insecticidal Activity of Methylene Chloride Extract of Cladosporium cladosporioides Secondary Metabolites against Aphis gossypii (Glov.)

Two different methods of chromatographic analysis have been used in this study for the phytochemical evaluation of main secondary metabolites in the aerial part of bee balm (Monarda fistulosa L.) as the non-officinal medicinal plant of the Lamiaceae Martinov family. The high performance thin layer chromatography (HPTLC) fingerprinting method was developed for the qualitative analyses of phenolic and non-polar compounds in the bee balm herb after its maceration in the solvents of different polarity. Such polyphenols as rosmarinic, caffeic and chlorogenic acids were authentically identified in the methanol extract of herb using HPTLC. Aromatic monoterpenoid thymol was identified by the HPTLC method in the extracts obtained with non-polar solvents (toluene, methylene chloride, and chloroform). 38 volatile compounds were determined in the methylene chloride extract of M. fistulosa herb by gas chromatography mass spectrometry (GC/MS); it was taken into account only components with the content more than 0.2 %. The GC/MS analysis showed that thymol (23.73 %), followed by carvacrol (10.09 %), p-cymene (9.74 %), and thymoquinone (8.52 %) were the major constituent of methylene chloride extract. Used chromatographic techniques may be recommended for the reliable phytochemical authentication of the M. fistulosa herb.

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Phytochemical investigation of the antisalmonellal effect of Cyperus sphacelatus Rottb. (Cyperaceae)

Current Chinese Science ◽

10.2174/2210298101666210302105522 ◽

2021 ◽

Vol 01 ◽

Author(s):

Napoleon A. Mfonku ◽

Gabriel T. Kamsu ◽

Norbert Kodjio ◽

Jie Ren ◽

James A. Mbah ◽

...

Keyword(s):

Typhoid Fever ◽

Antimicrobial Agents ◽

Methylene Chloride ◽

2D Nmr ◽

Sub Saharan Africa ◽

Resistant Isolate ◽

Bacterial Strains ◽

Phytochemical Investigation ◽

Methylene Chloride Extract ◽

Sub Saharan

Background: Typhoid fever is a major health burden in Sub-Saharan Africa. Conventional anti-typhoid drugs are becoming more and more unavailable to most patients in Africa due to the increased costs and emerging drug resistance. Therefore, there is a need for discovery of new antimicrobial agents to combat typhoid fever. Objective: This work aimed to investigate the bioactive components in Cyperus sphacelatus Rottb. (Cyperaceae) and test the antisalmonellal activity of the isolated compounds. Methods: Compound purification was done through column chromatography. Structure elucidation was accomplished based on the 1D and 2D NMR, IR and mass spectra. The biological assay was done using five bacterial strains, including Salmonella enterica subsp. enterica sérovars Typhi ATCC 6539 (STS), S. enterica subsp. enterica sérovars Typhi (ST), S. enteritidis (STE), S. enterica subsp. enterica sérovars Typhimurim (STM), and a resistant isolate of S. enterica subsp. enterica sérovars Typhi (ST566). Results: Three natural products were isolated from the methylene chloride extract of the rhizomes of C. sphacelatus, including a new furanoquinone, scabequinon-6(14)-ene (1) and two known compounds, cyperotundone (2) and vanillin (3). Compound 1 showed moderate antisalmonellal activity, with a minimal inhibitory concentration (MIC) of 32 µg/mL against STM and STS. The best inhibitory result was obtained with compound 2 on STM with a MIC of 8 µg/mL. Compound 2 also gave the best minimum bactericidal concentration (MBC) of 32 µg/mL on the STM strain. Conclusion: Discovery of the three antisalmonellal compounds from C. sphacelatus supports the addition of this plant to typhoid fever preparations.

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Cardioprotective effects of methanol/methylene chloride extract of Vitex cienkowskii (Verbebaceae) in l-NAME induced hypertension in rats

European Journal of Integrative Medicine ◽

10.1016/j.eujim.2013.07.005 ◽

2013 ◽

Vol 5 (6) ◽

pp. 519-526 ◽

Cited By ~ 2

Author(s):

Flaure M. Donfack Metchi ◽

Edwige Nguemfo ◽

Paulin Nana ◽

Joel R. Temdié ◽

Esther Ngo Lemba Tom ◽

...

Keyword(s):

Methylene Chloride ◽

Methylene Chloride Extract ◽

Induced Hypertension ◽

Cardioprotective Effects

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Chemical Constituents of the Aerial Parts of Euphorbia Nematocypha

Natural Product Communications ◽

10.1177/1934578x1601100210 ◽

2016 ◽

Vol 11 (2) ◽

pp. 1934578X1601100 ◽

Cited By ~ 1

Author(s):

Chen Xu ◽

Hai-Yang Jia ◽

Bo Zuo ◽

Zhi-Xin Liao ◽

Lan-Ju Ji ◽

...

Keyword(s):

Oleanolic Acid ◽

Ellagic Acid ◽

Betulinic Acid ◽

Methylene Chloride ◽

Chemical Constituents ◽

2D Nmr ◽

Cytotoxic Activities ◽

Aerial Parts ◽

Methylene Chloride Extract ◽

Oleanane Triterpenoid

Chemical constituents of the dried aerial parts of Euphorbia nematocypha were investigated. A new oleanane triterpenoid, trans, trans-2′,4′-hexadienedioicacid-1′-β-amyrin ester (1), together with, β-amyrin (2), β-amyrin acetate (3), betulinic acid (4), ellagic acid (5), oleanolic acid (6), β-sitosterol (7), kaempferol (8), quercetin (9), lupeol (10) and pseudo-taraxasterol (11) were isolated from the methylene chloride extract. Their structures were elucidated on the basis of extensive spectroscopic (1D- & 2D-NMR) and ESI-MS analysis and comparison with data reported in the literature. The new isolated triterpenoid showed moderate cytotoxic activities against HeLa and MCF-7cell lines.

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Biologically Active Aldehydes In Extracts Of Lactarius Pergamenus (Fr.) Fr Fresh Fruiting Bodies

METHODS AND OBJECTS OF CHEMICAL ANALYSIS ◽

10.17721/moca.2020.125-131 ◽

2020 ◽

Vol 15 (3) ◽

pp. 125-131

Author(s):

V.O. Antonyuk ◽

L.V. Panchak ◽

M.V. Tsivinska ◽

R.S. Stoika

Keyword(s):

Thin Layer ◽

Aromatic Amines ◽

Methylene Chloride ◽

Biologically Active ◽

Fruiting Bodies ◽

Highly Active ◽

Vegetable Kingdom ◽

Methylene Chloride Extract ◽

Colored Compound ◽

Selection Of

The biologically active aldehydes in extracts of fungi of the genus Lactarius were identified. It’s established that these substances are unstable, they are found in fresh and frozen fungi, but are absent in dried mushrooms and interact with 1,4-phenylenediamine to form a colored compound. Methylene chloride is the best extragent for these substances. TLC on silufol plates showed that there were several substances in Lactarius pergamenus fruiting bodies and they had varying degrees of stability. For selection of these substances, methylene chloride extract was separated on a column of silica gel. Fraction, which gave the most expressive reaction with 1,4-phenylenediamine on thin-layer chromatograms were analyzed by GC-MS both in the absence and in the presence of 1,4-phenylenediamine. As a result, it was found that 1,4-phenylenediamine or other aromatic amines interacted with highly active aldehydes, that were present in fruiting bodies. Among them 2,2-dimethylocta- 3,4-dienal was the most stable and was present in the biggest quantity. This substance very rarely occurs in the vegetable kingdom and in fungi extracts of Lactarius genus wasn’t previously described. Its possible function in fungi is prevention of damage by parasites and eating by animals.

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PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF ZILLA SPINOSA EXTRACTS AGAINST PATHOGENIC MICROORGANISMS

Journal of Medicinal Botany ◽

10.25081/jmb.2018.v2.987 ◽

2018 ◽

pp. 24 ◽

Cited By ~ 1

Author(s):

Oday Al-hadithy ◽

S. M. ALGHANEM ◽

M. MILAD

Keyword(s):

Antimicrobial Activity ◽

Ethyl Acetate ◽

Methyl Alcohol ◽

Methylene Chloride ◽

Dry Weight ◽

Klebsiella Pneumonia ◽

Phytochemical Analysis ◽

Pathogenic Microorganisms ◽

Alcohol Extract ◽

Methylene Chloride Extract

Zilla spinosa is one of the most common plant species of family Brassicaceae, due to its important uses in the folk medicinal. The present study was aimed to determine some active constituents and to test antimicrobial activity of different plant extracts against pathogenic microorganisms. Fresh plant was collected randomly from northern sector of Eastern Desert, Egypt (Cairo-Suez desert road) in the month of March 2018. Phytochemical analysis of Z. spinosa showed that, it was rich in saponins and tannins (34.68 and 32.82 mg/g dry weight, respectively). While, phenolics attained values (28.22 mg/g dry weight), alkaloids (17.56 mg/g dry weight) and flavonoids (11.22mg/g dry weight). In the present study, in case of petroleum ether extract the inhibition zone varied according to type of examined bacteria. Staphylococcus aureus was the most affected bacteria followed by Stroptococcus pyogenes, Klebsiella pneumonia and Bacillus subtilis. Methylene chloride extract does not affect both K. pneumoniae and Escherichia coli but prevents others. The ethyl acetate extract inhibit S. aureus, S. pyogenes and B. subtilis. The acetone extract inhibited all the bacteria except E. coli and K. pneumonia. Methyl alcohol extract inhibited all bacteria with different inhibition zones except B. subtilis. On the other hand, Methylene chloride extract has effect on Aspergillus fumigatus. The ethyl acetate and methyl alcohol extract inhibited the growth of all tested fungi except A. niger. Acetone inhibited the growth of Mucor spp. and Candidia albicans.

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Ferulol and epi-Samarcandin, Two New Sesquiterpene Coumarins from Ferula Sinaica

Natural Product Communications ◽

10.1177/1934578x0700200502 ◽

2007 ◽

Vol 2 (5) ◽

pp. 1934578X0700200

Author(s):

Ahmed A. Ahmed ◽

Abou El-Hamd H. Mohamed ◽

Mohamed H. Abd El-Razek ◽

Mohamed-Elamir F. Hegazy

Keyword(s):

Spectral Data ◽

Structure Elucidation ◽

Methylene Chloride ◽

2D Nmr ◽

Carbon Skeleton ◽

Methylene Chloride Extract

Re-investigation of the methylene chloride extract of the root of Ferula sinaica gave a sesquiterpene coumarin with a rare carbon skeleton and 3′,9′-di- epi-samarcandin. The structure elucidation was determined by MS; 1H- and 13C- 1D and 2D NMR spectral data.

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