Chromatographic Profiles of the main Secondary Metabolites in the Monarda fistulosa L. Aerial Part

2021 ◽  
pp. 2179-2184
Author(s):  
Mariia Shanaida ◽  
Izabela Jasicka-Misiak ◽  
Marietta Bialon ◽  
Olha Korablova ◽  
Piotr P. Wieczorek
Keyword(s):  
Secondary Metabolites ◽  
High Performance ◽  
Aerial Part ◽  
Methylene Chloride ◽  
Polar Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Monarda Fistulosa ◽  
Methylene Chloride Extract ◽  
Layer Chromatography

Two different methods of chromatographic analysis have been used in this study for the phytochemical evaluation of main secondary metabolites in the aerial part of bee balm (Monarda fistulosa L.) as the non-officinal medicinal plant of the Lamiaceae Martinov family. The high performance thin layer chromatography (HPTLC) fingerprinting method was developed for the qualitative analyses of phenolic and non-polar compounds in the bee balm herb after its maceration in the solvents of different polarity. Such polyphenols as rosmarinic, caffeic and chlorogenic acids were authentically identified in the methanol extract of herb using HPTLC. Aromatic monoterpenoid thymol was identified by the HPTLC method in the extracts obtained with non-polar solvents (toluene, methylene chloride, and chloroform). 38 volatile compounds were determined in the methylene chloride extract of M. fistulosa herb by gas chromatography mass spectrometry (GC/MS); it was taken into account only components with the content more than 0.2 %. The GC/MS analysis showed that thymol (23.73 %), followed by carvacrol (10.09 %), p-cymene (9.74 %), and thymoquinone (8.52 %) were the major constituent of methylene chloride extract. Used chromatographic techniques may be recommended for the reliable phytochemical authentication of the M. fistulosa herb.

scholarly journals PHYTOCHEMICAL ANALYSIS OF SECONDARY METABOLITES OF SATUREJA HORTENSIS L.

2017 ◽  
Vol 9 (2) ◽  
pp. 315
Author(s):  
Mariya Shanaida ◽  
Iryna Ivanusa ◽  
Ivanna Kernychna
Keyword(s):  
Secondary Metabolites ◽  
Essential Oil ◽  
Essential Oils ◽  
High Performance ◽  
Aerial Part ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Satureja Hortensis ◽  
Main Component ◽  
Essential Oil Yield

<p><strong>Objective: </strong>The present study was designated to analyse the essential oils and tannins as important secondary metabolites of the aerial part of <em>Satureja hortensis</em>.</p><p><strong>Methods: </strong>The chemical composition of <em>S</em><em>. </em><em>hortensis</em> herb was investigated using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The essential oil was obtained by hydrodistillation method.</p><p><strong>Results: </strong>The essential oil yield in the aerial part of<em> </em><em>S</em><em>. </em><em>hortensis</em><strong> </strong>was 1.61%. GC-MS analysis of the essential oils identified the presence of 29 components. Carvacrol (76.16%), as the main component of essential oils, belongs to the group of aromatic compounds. Eight tannin components identified by HPLC and epigallocatechin (130.91х10<sup>-2</sup>%) are prevalent among them.</p><p><strong>Conclusion</strong>:<strong> </strong><em>S. hortensis</em> was found to possess considerable amount of phytoconstituents such as essential oils and tannins. The results of this research will help to study pharmacological properties of the investigated plant and to prevent possible adulteration with other plants.</p>

Detection of the toxic substance dibutyl phthalate in Antarctic krill

2017 ◽  
Vol 29 (6) ◽  
pp. 511-516 ◽  
Author(s):  
Xiangning Han ◽  
Daicheng Liu
Keyword(s):  
High Performance ◽  
Dibutyl Phthalate ◽  
Antarctic Krill ◽  
Toxic Substance ◽  
Dioctyl Phthalate ◽  
Gas Chromatography Mass Spectrometry ◽  
Coastal Marine ◽  
Freeze Dried ◽  
Coastal Marine Ecosystems ◽  
Layer Chromatography

AbstractHigh-performance thin layer chromatography was performed to investigate the potential presence of four phthalic acid esters, dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP) and dioctyl phthalate (DEHP), in Antarctic krill. The results revealed that in freeze-dried Antarctic krill levels of DBP (0.1043±0.0005 mg g-1 (104.3±0.05 mg kg-1)) were high. The structure of DBP in Antarctic krill was determined by gas chromatography-mass spectrometry. Its existence is of concern based on demonstrated harmful effects to animals and plants as Antarctic krill is a key part of the food chain in Antarctic coastal marine ecosystems. The adverse effects of DBP on Antarctic krill and the source of DBP should be explored in further research.

scholarly journals Quantitative Analysis of Sulpiride and Impurities of 2-Aminomethyl-1-Ethylpyrrolidine and Methyl-5-Sulphamoyl-2-Methoxybenzoate in Pharmaceuticals by High-Performance Thin-Layer Chromatography and Scanning Densitometry

1999 ◽  
Vol 82 (4) ◽  
pp. 825-829 ◽  
Author(s):  
Danica Agbaba ◽  
Tatjana Miljkovic ◽  
Valentina Marinkovic ◽  
Dobrila Zivanov-Stakic ◽  
Sote Vladimirov
Keyword(s):  
Quantitative Analysis ◽  
Thin Layer ◽  
High Performance ◽  
Methylene Chloride ◽  
Solvent System ◽  
Ammonia Solution ◽  
Dosage Forms ◽  
Raw Material ◽  
Layer Chromatography

Abstract A simple and reliable thin-layer chromatographic method for determining sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate was developed and validated. A methylene chloride–methanol–ammonia solution (25%; 18 + 2.8 + 0.4, v/v) solvent system is used for separation and quantitative evaluation of chromatograms. The chromatographic plate is first scanned at 240 nm to locate chromatographic zones corresponding to sulpiride and methyl-5-sulphamoyl-2-methoxybenzoate. Then 2-aminomethyl-1-ethylpyrrolidine is derivatized in situ with ninhydrin, and resulting colored spots are measured at 500 nm. The method is reproducible and convenient for quantitative analysis and purity control of sulpiride in its raw material and in its dosage forms.

scholarly journals Cycloartane-Type Triterpenes and Botanical Origin of Propolis of Stingless Indonesian Bee Tetragonula sapiens

Plants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 57 ◽  
Author(s):  
Niken Pujirahayu ◽  
Toshisada Suzuki ◽  
Takeshi Katayama
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Mangifera Indica ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Botanical Origin ◽  
Characteristic Peak ◽  
Hplc Chromatogram ◽  
Main Components ◽  
Layer Chromatography

This study clarifies the chemical constituents and botanical origin of Tetragonula sapiens Cockerell bee propolis collected from Southeast Sulawesi, Indonesia. Propolis samples and resin of Mangifera indica were extracted with 99% ethanol to obtain an ethanol extract of propolis (EEP) and an ethanol extract of M. indica resin (EEM). Column chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were developed and used for the separation and isolation of compounds from the ether-soluble fraction. The structure of the compounds was determined by nuclear magnetic resonance (NMR) spectroscopic analysis, and their molecular weight analyzed by gas chromatography–mass spectrometry (GC–MS). The HPLC chromatogram of the EEP was then compared with the HPLC chromatogram of EEM to investigate the botanical origin of propolis. Five compounds were isolated from the EEP, and their structures were determined as mangiferolic acid, cycloartenol, ambonic acid, mangiferonic acid, and ambolic acid, which are cycloartane-type triterpenes. The characteristic peak of the HPLC chromatograms of EEP and EEM showed a similar pattern, which is that the main components of propolis were also found in M. indica resin. These results suggested that the propolis from Southeast Sulawesi was rich in cycloartane-type triterpenes, and the plant source of the propolis could be Mangifera indica (mango).

Bioremediation of synthetic fatliquors under microaerobic condition

2016 ◽  
Vol 75 (5) ◽  
pp. 1118-1127 ◽  
Author(s):  
B. Umamaheswari ◽  
K. Priya ◽  
Rama Rajaram
Keyword(s):  
Oxygen Demand ◽  
Polar Compounds ◽  
Industrial Applications ◽  
Gas Chromatography Mass Spectrometry ◽  
Microaerobic Condition ◽  
Wide Range ◽  
End Products ◽  
Emulsifying Agent ◽  
Microaerobic Conditions ◽  
Layer Chromatography

Synthetic fatliquors are useful as a fatliquoring agent, flotation agent and emulsifying agent in a wide range of industrial applications such as leather, pharmacy and farm chemicals. These fatliquors remain recalcitrant to natural biota in existing treatment plants. In the present study, the isolated microaerophilic Serratia sp. HA1 strain CSMB3 is capable of utilizing structurally different fatliquors as the sole substrate for their growth under microaerobic conditions. Degradation of vegetable fatliquors was observed from 95 to 97% in terms of lipids, with the production of lipase at 72 h. Degradation of synthetic fatliquors was observed in terms of chemical oxygen demand from 85% to a minimum of 25%. It is in the order of sulfited/sulfated fatliquors &gt; sulfochlorinated fatliquors &gt; chlorinated fatliquors. A thin layer chromatography chromatogram confirmed the degradation of non polar fatliquor to polar compounds. Production of the red pigment prodigiosin in synthetic fatliquors enhanced the growth of the isolate. Fourier transform infrared spectroscopy (FTIR) confirmed the bioremediation of sulfochlorinated fatliquor into lipids and fatty acids and gas chromatography–mass spectrometry (GC-MS) results confirmed that alcohols and esters are the final end products. Thus the isolated strain CSMB3 may be used in the treatment of wastewaters containing vegetable and synthetic fatliquors.

scholarly journals Isolation and purification of plant secondary metabolites using column-chromatographic technique

2016 ◽  
Vol 11 (4) ◽  
pp. 844 ◽  
Author(s):  
Vivek K. Bajpai ◽  
Rajib Majumder ◽  
Jae Gyu Park
Keyword(s):  
Secondary Metabolites ◽  
High Performance ◽  
Plant Secondary Metabolites ◽  
Biologically Active ◽  
Chromatographic Technique ◽  
Isolation And Purification ◽  
Chromatographic Techniques ◽  
Visual Demonstration ◽  
Layer Chromatography ◽  
Column Chromatographic

<p>Chromatographic techniques have significant role in natural products chemistry as well as contribute dramatically in the discovery of novel and innovative compounds of pharmaceutical and biomedical importance. This study focused on step-by-step visual demonstration of fractionation and isolation of biologically active plant secondary metabolites using column-chromatographic techniques. Isolation of bioactive compounds using column-chromatographic involves: a) Preparation of sample; b) Packing of column; c) Pouring of sample into the column; d) Elution of fractions; and e) Analysis of each fractions using thin layer chromatography. However, depending on nature of research, compounds can be further purified using high performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) spectral analyses.</p><p><strong>Video Clips</strong></p><p><a href="https://www.youtube.com/v/pr8mrBoI8xA">Part 1:</a> 3 min 45 sec</p><p><a href="https://www.youtube.com/v/rYrfClKn-og">Part 2:</a> 6 min 21 sec</p><p><a href="https://www.youtube.com/v/kffHXxuPwbo">Part 3</a>: 4 min 45 sec</p>

Antifungal Antibiotic from the Mushroom Agrocybe aegerita (BRIG.) SING.

1992 ◽  
Vol 57 (3) ◽  
pp. 590-603 ◽  
Author(s):  
Karel Stránský ◽  
Marta Semerdžieva ◽  
Miroslav Otmar ◽  
Želimír Procházka ◽  
Miloš Buděšínský ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Performance ◽  
Preparative Thin Layer Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Agrocybe Aegerita ◽  
Antifungal Antibiotic ◽  
Antibiotic Compounds ◽  
Structural Formulae ◽  
Layer Chromatography

An extract from a submersed culture of the mushroom Agrocybe aegerita (BRIG.) SING., containing antifungal antibiotic compounds was chromatographed on a silica gel column. Compounds from fractions which displayed the highest biological activity were concentrated and isolated by means of preparative thin-layer chromatography and preparative high-performance liquid chromatography, and were further characterized by means of gas chromatography, mass spectrometry and nuclear magnetic resonance. They are sesquiterpenic diols predominantly with an illudine skeleton. Structural formulae are proposed for some of them.

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