Evaluation of antibacterial activity of Citrus aurantium L. leaf extracts on bacteria isolated from blood of Hepatitis B positive individuals in Ondo State, Nigeria

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88

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Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

BioMed Research International ◽

10.1155/2014/521287 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 14

Author(s):

Somayeh Razmavar ◽

Mahmood Ameen Abdulla ◽

Salmah Binti Ismail ◽

Pouya Hassandarvish

Keyword(s):

Antibacterial Activity ◽

Ethanol Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Phytochemical Screening ◽

Bioactive Constituents ◽

Disc Diffusion Method ◽

Leaf Extracts ◽

Baeckea Frutescens ◽

High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

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Antibacterial Activity of Oenothera rosea (L ’Hér) Leaf Extracts

British Journal of Medicine and Medical Research ◽

10.9734/bjmmr/2012/1480 ◽

2012 ◽

Vol 2 (3) ◽

pp. 396-404 ◽

Cited By ~ 5

Author(s):

Ricardo Gomez-Flores

Keyword(s):

Antibacterial Activity ◽

Leaf Extracts

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Antimicrobial activity of leaf and root extracts of tough lovegrass

Comunicata Scientiae ◽

10.14295/cs.v7i4.1845 ◽

2017 ◽

Vol 7 (4) ◽

pp. 420 ◽

Cited By ~ 1

Author(s):

Adriana Favaretto ◽

Fabiana Tonial ◽

Charise Dallazem Bertol ◽

Simone Meredith Scheffer-Basso

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antifungal Activity ◽

Staphylococcus Epidermidis ◽

Phytopathogenic Fungus ◽

Leaf Extracts ◽

Reduced Pressure ◽

Root Extracts ◽

Xanthomonas Translucens ◽

Microdilution Broth

This study aimed to evaluate tough lovegrass leaf and root extracts antimicrobial activity. The extracts (plant material: solvent, 1:10) were prepared by maceration with methanol:water (1:1) during ten days followed by a concentration in a rotary evaporator under reduced pressure. The extracts were resuspended in water containing 1% of dimethylsulfoxide (DMSO) to obtain a final concentration of 100 mg/mL and then filtered through a sterilizing membrane with 0.22μm. The antibacterial activity of the leaf and root extracts were evaluated against pathogenic and phytopathogenic bacteria by agar well diffusion and microdilution broth methods for the minimum inhibitory concentrations (MIC) determination. The antifungal activity of tough lovegrass leaf and root extracts were evaluated by micelial growth inhibition and conidial germination inhibition. The extracts presented low antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Xanthomonas translucens, but the leaf extracts presented significant antifungal activity against the phytopathogenic fungus Drechslera tritici-repentis. The results support the continuity of the study in improving the effectiveness of the active extract for a possible use in pharmacology and agronomy and in attempting to determine the probable active antimicrobial compound.

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GREEN SYNTHESIS AND CHARACTERIZATION OF ZnO NANOPARTICLES FROM LEAF EXTRACTS OF BARRINTONIA ACUTANGULA AND ITS ANTIBACTERIAL ACTIVITY

RASAYAN Journal of Chemistry ◽

10.31788/rjc.2021.1436332 ◽

2021 ◽

Vol 14 (03) ◽

Author(s):

S. Renjusha ◽

P.H. Vaisakh

Keyword(s):

Antibacterial Activity ◽

Green Synthesis ◽

Zno Nanoparticles ◽

Synthesis And Characterization ◽

Leaf Extracts

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Synthesis of zinc oxide and silver nanoparticles using ficus palmata - Forssk leaf extracts and assessment of antibacterial activity

Environmental Engineering Research ◽

10.4491/eer.2020.454 ◽

2020 ◽

Vol 26 (6) ◽

pp. 200454-0

Author(s):

Sabaoon Shamshad ◽

Jamshaid Rashid ◽

Ihsan-ul-haq ◽

Naseem Iqbal ◽

Saif Ullah Awan

Keyword(s):

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Zinc Oxide ◽

Antibacterial Activity ◽

Zinc Oxide Nanoparticles ◽

Inhibitory Concentration ◽

Oxide Nanoparticles ◽

Leaf Extracts ◽

E Coli ◽

Ficus Palmata

Multidrug resistance of bacteria is an emerging human health hazard and warrants development of novel antibacterial agents with more effective mode of action. Here, zinc oxide and silver nanomaterials were prepared using Ficus palmata Forssk leaf extract with efficient antibacterial activity. SEM coupled with EDS confirmed the spherical symmetry with average particle diameter 50 to 65 nm while the XRD confirmed crystalline face centered cubic structure of silver and hexagonal crystallize phase of zinc oxide nanoparticles. Antibacterial activity was evaluated for 8 pathogenic bacterial strains including 3 drug resistant pathogenic strains. The nanoparticles showed enhanced growth inhibition for resistant strains in comparison with the broad-spectrum antibiotics i.e. roxithromycin and cefixime. Minimum inhibitory concentration in μg.mL-1 of silver nanoparticles was found to be as low as 33.3 for resistant Streptococcus haemolyticus; 11.1 for Staphylococcus aureus and E Coli; and 3.7 μg.mL-1 for resistant Pseudomonas aeruginosa. Similarly, the minimum inhibitory concentration of zinc oxide nanoparticles was found to be 100 μg.mL-1 against resistant Streptococcus haemolyticus and Staphylococcus aureus; 11.1 μg.mL-1 for resistant Pseudomonas aeruginosa; and 3.7 μg.mL-1 against resistant E coli. Ficus palmata Forssk leaf extracts can be explored effectively for synthesizing active antibacterial nanomaterials as a non-toxic and environmentally benign synthesis route.

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