scholarly journals Synthesis of zinc oxide and silver nanoparticles using ficus palmata - Forssk leaf extracts and assessment of antibacterial activity

2020 ◽  
Vol 26 (6) ◽  
pp. 200454-0
Author(s):  
Sabaoon Shamshad ◽  
Jamshaid Rashid ◽  
Ihsan-ul-haq ◽  
Naseem Iqbal ◽  
Saif Ullah Awan
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Inhibitory Concentration ◽  
Oxide Nanoparticles ◽  
Leaf Extracts ◽  
E Coli ◽  
Ficus Palmata

Multidrug resistance of bacteria is an emerging human health hazard and warrants development of novel antibacterial agents with more effective mode of action. Here, zinc oxide and silver nanomaterials were prepared using Ficus palmata Forssk leaf extract with efficient antibacterial activity. SEM coupled with EDS confirmed the spherical symmetry with average particle diameter 50 to 65 nm while the XRD confirmed crystalline face centered cubic structure of silver and hexagonal crystallize phase of zinc oxide nanoparticles. Antibacterial activity was evaluated for 8 pathogenic bacterial strains including 3 drug resistant pathogenic strains. The nanoparticles showed enhanced growth inhibition for resistant strains in comparison with the broad-spectrum antibiotics i.e. roxithromycin and cefixime. Minimum inhibitory concentration in μg.mL<sup>-1</sup> of silver nanoparticles was found to be as low as 33.3 for resistant Streptococcus haemolyticus; 11.1 for Staphylococcus aureus and E Coli; and 3.7 μg.mL<sup>-1</sup> for resistant Pseudomonas aeruginosa. Similarly, the minimum inhibitory concentration of zinc oxide nanoparticles was found to be 100 μg.mL<sup>-1</sup> against resistant Streptococcus haemolyticus and Staphylococcus aureus; 11.1 μg.mL<sup>-1</sup> for resistant Pseudomonas aeruginosa; and 3.7 μg.mL<sup>-1</sup> against resistant E coli. Ficus palmata Forssk leaf extracts can be explored effectively for synthesizing active antibacterial nanomaterials as a non-toxic and environmentally benign synthesis route.

scholarly journals Camellia sinensis (Green Tea) Mediated Synthesis of Zinc Oxide Nanoparticles and Detect its Antibacterial Activity against Escherichia coli, Staphylococcus aureus and Acinetobacter baumannii

2017 ◽  
Vol 11 (1) ◽  
pp. 34-40
Author(s):  
Israa Ali Zaidan Al-Ogaidi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Acinetobacter Baumannii ◽  
Green Tea ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Pathogenic Bacteria ◽  
Oxide Nanoparticles

In the current study, synthesis and characterization of Zinc oxide nanoparticles (ZnONPs) and its application as anti-pathogenic bacteria were investigated.  ZnO which has been prepared by using aqueous of green tea leaves extract (Camellia sinensis) as a reducing agent. The wavelength range was measured by Ultraviolet–visible spectroscopy (UV-Vis) for monitoring the formation of the nanoparticles, which showed sharp peak at 360 nm. The average size and shape of the nanoparticles were detected by using Atomic Force Microscopy (AFM) which was 88 nm with spherical shape. Fourier transform–infrared (FTIR). FT-IR spectra was documented for the ZnO nanoparticles synthesized by green tea extract to detect the biomolecules involved in the synthesis process. The antibacterial activity of crystal Zinc Oxide (ZnO) nanoparticles was explored against pathogenic bacteria that included Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. The antibacterial test was conducted in solid media using different concentrations of ZnO and disk diffusion method, 100 µg/ml presented the best antibacterial activity, and further studies on the damage of bacterial genomic DNA of Escherichia coli and Acinetobacter baumannii were carried out using gel electrophoresis exposed the DNA fragment bands, this activity may be caused by the interactions between the surface charge of cell and nanoparticles. Reactive oxygen species (ROS) properties of the particles might disturb cell wall and great antimicrobial action

scholarly journals Green Synthesis of Zinc Oxide Nanoparticles and their Antibacterial Properties using Plant Extract of Aristolochia elegans

2020 ◽  
Vol 32 (10) ◽  
pp. 2589-2593
Author(s):  
Juhi Aggarwal ◽  
Tanveer Alam
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Property ◽  
Antibacterial Properties ◽  
Oxide Nanoparticles ◽  
Gram Negative ◽  
E Coli ◽  
Ft Ir

Present paper deals with the synthesis of zinc oxide nanoparticles (ZnONPs) using leaf extract of Aristolochia elegans and study of antibacterial property for some human bacterial pathogens. The ZnONPs synthesized were characterized using UV-Vis, FT-IR, XRD, EDX, TEM and SEM techniques. The synthesized ZnONP having a crystallite size of 20.1 nm exhibited a distinct absorption peak maxima at 358 nm. The ZnONPs synthesized using the extract of A. elegans have shown antibacterial activity against M. luteus, S. aureus (Gram-positive), E. coli and P. aeruginosa (Gram-negative).

scholarly journals Enhanced antibacterial activity of capped zinc oxide nanoparticles: A step towards the control of clinical bovine mastitis

2019 ◽  
Vol 12 (8) ◽  
pp. 1225-1232 ◽  
Author(s):  
H. F. Hozyen ◽  
E. S. Ibrahim ◽  
E. A. Khairy ◽  
S. I. El-Dek
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Combustion Reaction ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
E Coli ◽  
Auto Combustion

Background and Aim: Bovine mastitis is the costliest prevalent disease in the dairy sector due to the limitations of conventional treatments. Zinc oxide nanoparticles (ZnO-NPs) have been regarded as safe and economical antibacterial candidates against several microorganisms, but the tendency of these particles to aggregate is a major barrier to their application. This study aimed to enhance the antibacterial efficiency of ZnO-NPs against some bacterial agents, causing bovine mastitis. Materials and Methods: A total of 24 milk samples out of 300 cases from Nubaria farm, Beheira Governorate, Egypt, were collected from cows with clinical mastitis. ZnO-NPs were fabricated by a sonochemical method using starch as a capping agent and by an auto-combustion reaction using glycine as a fuel. The two preparations of synthesized ZnO-NPs at different concentrations were assessed for their antimicrobial activities in vitro against Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae isolated from milk of affected cows. Results: Sonochemically synthesized capped ZnO-NPs were dispersed and non-agglomerated in comparison with aggregated uncapped ZnO-NPs prepared by an auto-combustion reaction. Capped dispersed ZnO-NPs showed higher antibacterial activity against S. aureus, E. coli, and K. pneumoniae than particles synthesized by the auto-combustion reaction at same concentrations. However, the zone of inhibition for dispersed and agglomerated ZnO-NPs was concentration-dependent. In addition, Gram-positive S. aureus exhibited higher resistance to ZnO-NPs synthesized by both methods than Gram-negative E. coli and K. pneumoniae. Conclusion: Dispersed, non-agglomerated ZnO-NPs fabricated using starch as a capping agent under sonochemical irradiation could potentially be regarded as highly effective and inexpensive antimicrobial agents against S. aureus, E. coli, and K. pneumoniae for the management of bovine mastitis. Keywords: antibacterial activity, clinical mastitis, dairy cows, zinc oxide nanoparticles.

The use of zinc oxide nanoparticles to enhance the antibacterial properties of light-activated polydimethylsiloxane containing crystal violet

RSC Advances ◽  
2015 ◽  
Vol 5 (12) ◽  
pp. 8806-8813 ◽  
Author(s):  
Ekrem Ozkan ◽  
Feyza Tunali Ozkan ◽  
Elaine Allan ◽  
Ivan P. Parkin
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Crystal Violet ◽  
Zinc Oxide Nanoparticles ◽  
Antibacterial Properties ◽  
Oxide Nanoparticles ◽  
Step Method ◽  
E Coli ◽  
Modified Polymer

Crystal violet–ZnO mixtures were incorporated into PDMS by a simple two step method. The modified polymer demonstrated significant antibacterial activity againstE. coliandS. aureus, showing possibly the most potent light-induced antibacterial polymer reported to date.

A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Quercus Robur ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Oxide Nanoparticles ◽  
Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

scholarly journals Scolicidal activity of biosynthesized zinc oxide nanoparticles by Mentha longifolia L. leaves against Echinococcus granulosus protoscolices

2021 ◽  
Author(s):  
Bushra H. Shnawa ◽  
Samir M. Hamad ◽  
Azeez A. Barzinjy ◽  
Payman A. Kareem ◽  
Mukhtar H. Ahmed
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Public Health Problem ◽  
Analytical Techniques ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Leaf Extracts ◽  
X Ray ◽  
Morphological Alterations ◽  
Mentha Longifolia

AbstractCystic echinococcosis is a public health problem in developing countries that practice sheep breeding extensively. In the current study, the protoscolicidal activity of biosynthesized zinc oxide nanoparticles (ZnO NPs) derived from Mentha longifolia L. leaf extracts was investigated. The resultant ZnO NPs were characterized by means of various analytical techniques, such as ultraviolet–visible (UV–Vis) spectrometry, Fourier transform infrared (FT-IR) spectrophotometry, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) analysis. The results showed that the ZnO NP had the highest scolicidal activity at 400 ppm concentration after 150 min of exposure time, showing 100% mortality rate. The treated protoscolices exhibited loss of viability with several morphological alterations. Hence, an easy and effective green synthesis of ZnO NPs, with efficient scolicidal potential, is reported in this study.

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