The impact of precipitation and external carbon source addition on biological nutrient removal in activated sludge systems – experimental investigation and mathematical modeling

2012 ◽  
Vol 7 (1) ◽  
Author(s):  
J. Makinia ◽  
J. Drewnowski ◽  
M. Swinarski ◽  
K. Czerwionka ◽  
M. Kaszubowska ◽  
...  
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Carbon Sources ◽  
Full Scale ◽  
Biological Nutrient Removal ◽  
Batch Experiments ◽  
Carbon Addition ◽  
Reference Case ◽  
External Carbon Source ◽  
The Impact

The aim of this study was to determine the effects of chemical precipitation and addition of external carbon sources on the denitrification capability and enhanced biological phosphorus removal (EBPR) interactions at the ‘Wschod’ WWTP (600,000 PE) in Gdansk (northern Poland). For this purpose, different kinds of batch experiments were carried out with the settled wastewater (without pretreatment and after coagulation-flocculation) and external carbon sources (ethanol and fusel oil). Precipitation of colloidal and particulate organic fractions has a significant effect on denitrification and EBPR. The removal of these two fractions by coagulation-flocculation resulted in the reduced process rates (30–70%). The experimental investigations were supported by both lab-scale and full-scale simulations using a newly developed model as an expansion of the Activated Sludge Model No. 2d (ASM2d). The new model accurately predicted the effects of precipitation and external carbon addition in batch experiments. Full-scale simulations revealed that addition of external carbon source can compensate the effects of precipitation resulting in a similar NO3-N behavior compared to the reference case (without precipitation and external carbon addition). The combined effects of precipitation and addition of external carbon source resulted in a significantly different PO4-P behavior compared to the reference case.

The role of colloidal and particulate organic compounds in denitrification and EBPR occurring in a full-scale activated sludge system

2011 ◽  
Vol 63 (2) ◽  
pp. 318-324 ◽  
Author(s):  
J. Drewnowski ◽  
J. Makinia
Keyword(s):  
Activated Sludge ◽  
Carbon Sources ◽  
Treatment Plant ◽  
P Uptake ◽  
Full Scale ◽  
Biological Nutrient Removal ◽  
Batch Reactors ◽  
Biological Phosphorus Removal ◽  
Phosphate Release ◽  
Automatic Batch

The efficiencies of denitrification and enhanced biological phosphorus removal (EBPR) in biological nutrient removal (BNR) activated sludge systems are strongly dependent on the availability of appropriate carbon sources. Due to high costs of commercial compounds (such as methanol, ethanol, acetic acid etc.) and acclimation periods (usually) required, the effective use of internal carbon sources for denitrification is preferred. The aim of this study was to determine the immediate effects of slowly biodegradable substrates on the denitrification capability and phosphate release/uptake interactions for a full-scale biomass process from the “Wschod” wastewater treatment plant (WWTP) in Gdansk (Poland). Since it is hard to distinguish the slowly biodegradable substrate in a direct way, a novel procedure based on batch experiments was developed and implemented. The laboratory experiments were carried out in two parallel, fully automatic batch reactors with the settled wastewater without pretreatment and after coagulation-flocculation. The removal of colloidal and particulate fractions resulted in the reduced observed process rates, such as denitrification, phosphate release and phosphate uptake (under aerobic and anoxic conditions). The reduction ranged from approximately 14% for the anaerobic P release to approximately 46% for the anoxic P uptake.

scholarly journals The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Matuszewska ◽  
Tomasz Maciąg ◽  
Magdalena Rajewska ◽  
Aldona Wierzbicka ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Antimicrobial Activity ◽  
Carbon Source ◽  
Reference Genes ◽  
Plant Pathogens ◽  
Plant Protection ◽  
Carbon Sources ◽  
Unknown Compound ◽  
Fungal Plant Pathogens ◽  
The Impact

AbstractPseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7-hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for the antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.

scholarly journals Modelization of Nutrient Removal Processes at a Large WWTP Using a Modified ASM2d Model

2018 ◽  
Vol 15 (12) ◽  
pp. 2817 ◽  
Author(s):  
Jakub Drewnowski ◽  
Jacek Makinia ◽  
Lukasz Kopec ◽  
Francisco-Jesus Fernandez-Morales
Keyword(s):  
Nutrient Removal ◽  
Carbon Sources ◽  
Oxygen Demand ◽  
Full Scale ◽  
Biological Nutrient Removal ◽  
Organic Substrates ◽  
Modified Model ◽  
Rate Limiting Step ◽  
Uptake Rates ◽  
Rate Limiting

The biodegradation of particulate substrates starts by a hydrolytic stage. Hydrolysis is a slow reaction and usually becomes the rate limiting step of the organic substrates biodegradation. The objective of this work was to evaluate a novel hydrolysis concept based on a modification of the activated sludge model (ASM2d) and to compare it with the original ASM2d model. The hydrolysis concept was developed in order to accurately predict the use of internal carbon sources in enhanced biological nutrient removal (BNR) processes at a full scale facility located in northern Poland. Both hydrolysis concepts were compared based on the accuracy of their predictions for the main processes taking place at a full-scale facility. From the comparison, it was observed that the modified ASM2d model presented similar predictions to those of the original ASM2d model on the behavior of chemical oxygen demand (COD), NH4-N, NO3-N, and PO4-P. However, the modified model proposed in this work yield better predictions of the oxygen uptake rate (OUR) (up to 5.6 and 5.7%) as well as in the phosphate release and uptake rates.

scholarly journals Optimizing external carbon source addition in domestics wastewater treatment based on online sensoring data and a numerical model

2017 ◽  
Vol 75 (11) ◽  
pp. 2716-2725 ◽  
Author(s):  
Qibin Wang ◽  
Qiuwen Chen ◽  
Jing Chen
Keyword(s):  
Wastewater Treatment ◽  
Numerical Model ◽  
Carbon Source ◽  
Wastewater Treatment Plants ◽  
Pilot Scale ◽  
Fixed Dose ◽  
Carbon Addition ◽  
External Carbon Source ◽  
Wastewater Quality ◽  
Denitrification Reactor

The removal of total nitrogen in wastewater treatment plants (WWTPs) is often unsatisfactory for a variety of reasons. One possible measure to improve nitrogen removal is the addition of external carbon. However, the amount of carbon addition is directly related to WWTP operation costs, highlighting the importance of accurately determining the amount of external carbon required. The objective of this study was to obtain a low nitrate concentration in the anoxic zone of WWTPs efficiently and economically by optimizing the external carbon source dosage. Experiments were conducted using a pilot-scale pre-denitrification reactor at a Nanjing WWTP in China. External carbon source addition based on online monitoring of influent wastewater quality and a developed nitrification–denitrification numerical model was investigated. Results showed that carbon addition was reduced by 47.7% and aeration costs were reduced by 8.0% compared with those using a fixed-dose addition mode in the pilot reactor. The obtained technology was applied to the full-scale Jiangxinzhou WWTP in Nanjing with promising results.

scholarly journals Methane Emissions Driven by Adding a Gradient of Ethanol as Carbon Source in Integrated Vertical-Flow Constructed Wetlands

Water ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 1086
Author(s):  
Xiaoling Liu ◽  
Jingting Wang ◽  
Xiaoying Fu ◽  
Hongbing Luo ◽  
Bruce C. Anderson ◽  
...  
Keyword(s):  
Carbon Source ◽  
Constructed Wetlands ◽  
Control Experiment ◽  
Ethanol Concentration ◽  
Removal Rate ◽  
Carbon Sources ◽  
Vertical Flow ◽  
Emission Flux ◽  
Rate Range ◽  
External Carbon Source

This work aims to investigate the methane emissions from integrated vertical-flow constructed wetlands (IVCWs) when ethanol is added as an external carbon source. In this study, a gradient of ethanol (0, 2, 4, 8, 16 and 32 mmol/L) was added as the carbon source in an IVCW planted with Cyperus alternifolius L. The results showed that the methane emission flux at an ethanol concentration of 32 mmol/L was 32.34 g CH4 m−2 day−1 less than that of the control experiment (0 mmol/L) and that the methane emission flux at an ethanol concentration of 16 mmol/L was 5.53 g CH4 m−2 day−1 less than that at 0 mmol/L. In addition, variations in the water quality driven by the different ethanol concentrations were found, with a redox potential range of −64 mV to +30 mV, a pH range of 6.6–6.9, a chemical oxygen demand (COD) removal rate range of 41% to 78%, and an ammonia nitrogen removal rate range of 59% to 82% after the ethanol addition. With the average CH4-C/TOC (%) value of 35% driven by ethanol, it will be beneficial to understand that CH4-C/TOC can be considered an ecological indicator of anthropogenic methanogenesis from treatment wetlands when driven by carbon sources or carbon loading. It can be concluded that adding ethanol as an external carbon source can not only meet the water quality demand of the IVCW treatment system but also stimulate and increase the average CH4 emissions from IVCWs by 23% compared with the control experiment. This finding indicates that an external carbon source can stimulate more CH4 emissions from IVCWs and shows the importance of carbon sources during sewage treatment processes when considering greenhouse emissions from treated wetlands.

Iterative design of a nitrate controller using an external carbon source in an activated sludge process

1998 ◽  
Vol 37 (12) ◽  
pp. 95-102 ◽  
Author(s):  
Pericles R. Barros ◽  
Bengt Carlsson
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
High Performance ◽  
Controller Design ◽  
Wastewater Treatment Plants ◽  
Design Procedure ◽  
Pole Placement ◽  
Activated Sludge Process ◽  
Suggested Approach ◽  
External Carbon Source

More stringent effluent and cost requirements are increasing the need for better control of wastewater treatment plants. In an activated sludge process, the nitrogen removal efficiency may be improved by adding an external carbon source. In this paper, automatic control of the nitrate level by regulating external carbon flow is discussed. More specifically, an iterative tuning procedure for the controller is outlined. Iterative controller design schemes aim at tuning high performance controllers of low complexity using closed loop data. The basic strategy used in this paper is an iterative pole placement controller design procedure. The suggested approach is compared with conventional design in a simulation study.

Simultaneous removal of organic and strong nitrogen from sewage in a pilot-scale BNR process supplemented with food waste

2004 ◽  
Vol 49 (5-6) ◽  
pp. 257-264 ◽  
Author(s):  
S.R. Chae ◽  
S.H. Lee ◽  
J.O. Kim ◽  
B.C. Paik ◽  
Y.C. Song ◽  
...  
Keyword(s):  
Carbon Source ◽  
Food Waste ◽  
Nutrient Removal ◽  
Phosphorus Removal ◽  
Pilot Scale ◽  
Biological Nutrient Removal ◽  
Biological Phosphorus Removal ◽  
External Carbon Source ◽  
N Removal ◽  
Biological Phosphorus

As the sewerage system is incomplete, sewage in Korea lacks easily biodegradable organics for nutrient removal. In this country, about 11,400 tons of food waste of high organic materials is produced daily. Therefore, the potential of food waste as an external carbon source was examined in a pilot-scale BNR (biological nutrient removal) process for a half year. It was found that as the supply of the external carbon increased, the average removal efficiencies of T-N (total nitrogen) and T-P (total phosphorus) increased from 53% and 55% to 97% and 93%, respectively. VFAs (volatile fatty acids) concentration of the external carbon source strongly affected denitrification efficiency and EBPR (enhanced biological phosphorus removal) activity. Biological phosphorus removal was increased to 93% when T-N removal efficiency increased from 78% to 97%. In this study, several kinds of PHAs (poly-hydroxyalkanoates) in cells were observed. The observed PHAs was composed of 37% 3HB (poly-3- hydroxybutyrate), 47% 3HV (poly-3-hydroxyvalerate), 9% 3HH (poly-3-hydroxyhexanoate), 5% 3HO (poly-3-hydroxyoctanoate), and 2% 3HD (poly-3-hydroxydecanoate).

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