The Effect of Dissolved Nutrients and Inorganic Suspended Solids on the Survival of E. coli in Seawater

1991 ◽  
Vol 24 (2) ◽  
pp. 133-136 ◽  
Author(s):  
D. P. Milne ◽  
J. C. Curran ◽  
J. S. Findlay ◽  
J. M. Crowther ◽  
J. Bennett ◽  
...  
Keyword(s):  
Steady State ◽  
Glucose Concentration ◽  
Laboratory Experiments ◽  
Suspended Solids ◽  
Salinity Range ◽  
Survival Times ◽  
Dissolved Nutrients ◽  
E Coli ◽  
Solids Concentration ◽  
Low Concentrations

The effect of dissolved nutrients and inorganic suspended solids on E. coli inactivation has been examined in laboratory experiments. The work employed artificial seawater with dissolved glucose and peptone, laboratory processed suspended solids and chemostat steady state E. coli cultures to establish the effect of these parameters on the viability of an E. coli population. Initial E. coli concentration was 5 × 103 100ml−1, the temperatures 5 °C and 20°C, the suspended solids concentration range was 0 - 100mgl−1, glucose concentration l.0mgl −1, peptone concentration 9.0mgl−1, salinity range 27 - 32‰ and the experimental vessels were shielded from light. Previous work has shown that nutrient-free inorganic suspended solids, at low concentrations, markedly increased the survival of E. coli in seawater. The work presented here shows that the presence of dissolved nutrients greatly increased E. coli survival, in the absence of suspended solids. However at suspended solids concentrations of >5 - 12mgl−1survival time was greatly reduced; thereafter increasing suspended solids concentration in the range >12.5 - 100mgl−1, generally resulted in increasing survival times.

The Effect of Estuary Type Suspended Solids on Survival of E. coli in Saline Waters

1989 ◽  
Vol 21 (3) ◽  
pp. 61-65 ◽  
Author(s):  
D. P. Milne ◽  
J. C. Curran ◽  
J. S. Findlay ◽  
J. M. Crowther ◽  
S. G. Wallis
Keyword(s):  
Survival Time ◽  
Laboratory Experiments ◽  
Suspended Solids ◽  
Salinity Range ◽  
Survival Times ◽  
Chemical Parameters ◽  
Additional Increase ◽  
E Coli ◽  
Physical Chemical ◽  
Solids Concentration

The effect of estuary type suspended solids on E.coli inactivation has been examined in laboratory experiments. The work used artificial seawater, laboratory processed suspended solids and chemostat steady-state E.coli cultures to establish the effect of these physical/chemical parameters on the viability of an E.coli population. Initial E.coli concentrations were 5 × 103. 100ml−1, the temperatures employed were 10°C and 20°C, the suspended solids concentration range was 0 – l00mgl−1, salinity range 10 – 32%o, and the experimental vessels were completely shielded from light. The introduction of suspended solids at a low concentration (ie 5mgl−1) markedly increased the survival time of E.coli at the higher salinities (ie 32% o and 27%o). Suspended solids at higher concentrations (>12.5mgl−1) provided no additional increase in survival time. At low suspended solids concentrations (≤5mgl−1) decreasing salinity increased survival time; at higher suspended solids concentration (>12.5mgl−1) salinities in excess of a critical value, around 27%o, also increased survival times.

Treatment of tunnel wash water and implications for its disposal

2014 ◽  
Vol 69 (10) ◽  
pp. 2029-2035 ◽  
Author(s):  
M. Hallberg ◽  
G. Renman ◽  
L. Byman ◽  
G. Svenstam ◽  
M. Norling
Keyword(s):  
Traffic Safety ◽  
Urban Areas ◽  
Suspended Solids ◽  
Treatment Plant ◽  
Particulate Material ◽  
Wash Water ◽  
Polluted Water ◽  
Solids Concentration ◽  
Low Concentrations ◽  
Road Tunnels

The use of road tunnels in urban areas creates water pollution problems, since the tunnels must be frequently cleaned for traffic safety reasons. The washing generates extensive volumes of highly polluted water, for example, more than fivefold higher concentrations of suspended solids compared to highway runoff. The pollutants in the wash water have an affinity for particulate material, so sedimentation should be a viable treatment option. In this study, 12 in situ sedimentation trials were carried out on tunnel wash water, with and without addition of chemical flocculent. Initial suspended solids concentration ranged from 804 to 9,690 mg/L. With sedimentation times of less than 24 hours and use of a chemical flocculent, it was possible to reach low concentrations of suspended solids (<15 mg/L), PAH (<0.1 μg/L), As (<1.0 μg/L), Cd (<0.05 μg/L), Hg (<0.02 μg/L), Fe (<200 μg/L), Ni (<8 μg/L), Pb (<0.5 μg/L), Zn (<60 μg/L) and Cr (<8 μg/L). Acute Microtox® toxicity, mainly attributed to detergents used for the tunnel wash, decreased significantly at low suspended solids concentrations after sedimentation using a flocculent. The tunnel wash water did not inhibit nitrification. The treated water should be suitable for discharge into recipient waters or a wastewater treatment plant.

Immunogold labeling of CMP-KDO synthetase produced by recombinant E. Coli cells

1987 ◽  
Vol 45 ◽  
pp. 924-925
Author(s):  
F. A. Durum ◽  
R. G. Goldman ◽  
T. J. Bolling ◽  
M. F. Miller
Keyword(s):  
Inclusion Bodies ◽  
Large Scale ◽  
Recombinant Dna ◽  
Test System ◽  
Cell Protein ◽  
Gram Negative Bacteria ◽  
Cytoplasmic Inclusions ◽  
Isolation And Purification ◽  
E Coli ◽  
Low Concentrations

CMP-KDO synthetase (CKS) is an enzyme which plays a key role in the synthesis of LPS, an outer membrane component unique to gram negative bacteria. CKS activates KDO to CMP-KDO for incorporation into LPS. The enzyme is normally present in low concentrations (0.02% of total cell protein) which makes it difficult to perform large scale isolation and purification. Recently, the gene for CKS from E. coli was cloned and various recombinant DNA constructs overproducing CKS several thousandfold (unpublished data) were derived. Interestingly, no cytoplasmic inclusions of overproduced CKS were observed by EM (Fig. 1) which is in contrast to other reports of large proteinaceous inclusion bodies in various overproducing recombinant strains. The present immunocytochemical study was undertaken to localize CKS in these cells.Immune labeling conditions were first optimized using a previously described cell-free test system. Briefly, this involves soaking small blocks of polymerized bovine serum albumin in purified CKS antigen and subjecting them to various fixation, embedding and immunochemical conditions.

Calibration 7" – Cutthroat Flume as New Size for Discharge Measurement at Free Flow Condition

2020 ◽  
Vol 38 (12A) ◽  
pp. 1783-1789
Author(s):  
Jaafar S. Matooq ◽  
Muna J. Ibraheem
Keyword(s):  
Steady State ◽  
Flow Condition ◽  
Laboratory Experiments ◽  
Free Flow ◽  
Experimental Program ◽  
Steady State Flow ◽  
Empirical Formulas ◽  
State Flow ◽  
Operation Conditions ◽  
Throat Width

 This paper aims to conduct a series of laboratory experiments in case of steady-state flow for the new size 7 ̋ throat width (not presented before) of the cutthroat flume. For this size, five different lengths were adopted 0.535, 0.46, 0.40, 0.325 and 0.27m these lengths were adopted based on the limitations of the available flume. The experimental program has been followed to investigate the hydraulic characteristic and introducing the calibrated formula for free flow application within the discharge ranged between 0.006 and 0.025 m3/s. The calibration result showed that, under suitable operation conditions, the suggested empirical formulas can accurately predict the values of discharge within an error ± 3%.

Nitrogen Reduction – Volume Demand

1992 ◽  
Vol 25 (4-5) ◽  
pp. 233-240
Author(s):  
T. Palmgren
Keyword(s):  
Suspended Solids ◽  
Age Factors ◽  
Reduction Rate ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Aeration Tank ◽  
Nitrogen Reduction ◽  
Solids Concentration ◽  
Yearly Average ◽  
Nitrification Process

Due to the slow growth of nitrification bacteria at low temperatures, nitrogen reduction normally requires long hydraulic retention time during winter. Important for the nitrification process is the aerated sludge age. Factors influencing the sludge age are aerated volume, mixed liquor suspended solids concentration, organic loading and sludge yield. In an existing plant you cannot easily expand the volume and the load is difficult to decrease. But the suspended solids concentration can be increased by running the biological step with the contact stabilisation process. At the Käppala Association sewage treatment plant in Lidingö just outside Stockholm, one of the six aeration tanks has been reconstructed for full scale nitrogen removal experiments. In this tank the old aeration system has been replaced with rubber membrane diffusers. Further more there are several zones separated by walls in the tank. The tank can thereby be run with great flexibility. By running it with the contact stabilisation process, the sludge age has been improved by a factor between 1.5 and 2 and thereby it succeeds in keeping the nitrification bacteria in the system even during snow melting. At temperatures of about 9 °C and hydraulic retention times of less than 3 hours in the contact zone there has been a nitrification degree of up to 50 to 60 %. The experiment was conducted with a stabilisation zone of up to half the total volume of the aeration tank. The main purpose for the experiments during the winter seasons was to improve nitrification. Keeping the nitrifiers in the system had been a crucial problem during previous years. When the nitrifiers were lost with an increased flow and decreased temperature the nitrification process didn't restart until the temperature was increased and the load decreased. Usually this didn't occur until the middle of the summer meaning a loss of nitrification for up to six months. In Sweden there is a goal set for 50 % nitrogen reduction for the plants in the Stockholm region. At Käppala we manage to keep 60 to 70 % nitrogen reduction during the warm season, that is from July to December. If we can keep up the nitrification the whole year we can achieve 50 % as a yearly average under normal conditions even though we can't keep the nitrogen reduction rate as high during the cold season.

Experiments on high rate effluent filtration in the Netherlands

1998 ◽  
Vol 38 (3) ◽  
pp. 127-134
Author(s):  
Jaap H. J. M. van der Graaf ◽  
Arjen F. van Nieuwenhuijzen
Keyword(s):  
Wastewater Treatment ◽  
The Netherlands ◽  
Suspended Solids ◽  
Practical Experience ◽  
High Rate ◽  
Treated Water ◽  
Low Concentrations ◽  
Wastewater Treatment Effluent

As yet, filtration of wastewater treatment effluent has not been practised in the Netherlands. The main objections were the expected high costs. In order to gain practical experience an investigation programme studied the applicability and optimization of effluent filtration. Especially multi-layer filtration with the addition of ironchloride seemed to be very effective. Very low concentrations of suspended solids and phosphorus were achieved, even at high filtration rates (up to 30 m/h). This leads to an impressive reduction of expected costs, down to Dfl. 0.02/m3 (treated water).

scholarly journals Temporal Variability of Suspended-Solids Concentration in the Estuarine Channel of Patos Lagoon, Southern Brazil

Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 646
Author(s):  
Rafael André Ávila ◽  
Priscila Mulattieri Suarez Orozco ◽  
Mauro Michelena Andrade ◽  
Osmar Olinto Möller
Keyword(s):  
Temporal Variability ◽  
Suspended Solids ◽  
Southern Brazil ◽  
Patos Lagoon ◽  
Reliable Technique ◽  
Acoustic Data ◽  
Solids Concentration ◽  
Estuarine Hydrodynamics ◽  
Inflow Conditions

The assessment of suspended-solids dynamics is crucial for the effective monitoring of estuarine environments. As the recurring in-situ sampling is usually problematic, the calibration of the backscattering from acoustic Doppler profilers has shown to be a reliable technique to estimate the suspended-solids concentration (SSC) in estuaries and rivers. In this study, we obtained a linear model that provides SSC estimates for the estuarine channel of Patos Lagoon by calibrating turbidity and acoustic data with in-situ concentration samples. The model output was analyzed in terms of its relationship with estuarine hydrodynamics and temporal variability. In this estuary, the supply of suspended solids is known to be due the runoff from its main tributaries, but also through the exchanges between the estuary and the coastal ocean. Both sources provide sediments and organic solids which affect water quality, geomorphology, and harbor operations. Results show that SSC is strongly linked to estuarine hydrodynamics, where concentrations increase with streamflow. During outflow periods, higher concentrations are associated with river runoff, whereas with inflow conditions they are induced by southern and southwesterly winds. However, relationship between SSC and streamflow is asymmetrical, meaning that the largest concentrations are majorly linked to outflow currents and downstream transport.

Supradegeneracy and the Second Law of Thermodynamics

2020 ◽  
Vol 45 (2) ◽  
pp. 121-132
Author(s):  
Daniel P. Sheehan
Keyword(s):  
Steady State ◽  
Statistical Mechanics ◽  
Population Inversion ◽  
Laboratory Experiments ◽  
Second Law Of Thermodynamics ◽  
Second Law ◽  
Boltzmann Factor ◽  
Energy Currents ◽  
Non Equilibrium

AbstractCanonical statistical mechanics hinges on two quantities, i. e., state degeneracy and the Boltzmann factor, the latter of which usually dominates thermodynamic behaviors. A recently identified phenomenon (supradegeneracy) reverses this order of dominance and predicts effects for equilibrium that are normally associated with non-equilibrium, including population inversion and steady-state particle and energy currents. This study examines two thermodynamic paradoxes that arise from supradegeneracy and proposes laboratory experiments by which they might be resolved.

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