Filamentous Bulking and Hindrance of Phosphate Removal Due to Sulfate Reduction in Activated Sludge

1991 ◽  
Vol 23 (4-6) ◽  
pp. 927-935 ◽  
Author(s):  
Ryoko Ikemoto Yamamoto ◽  
Tomoaki Komori ◽  
Saburo Matsui
Keyword(s):  
Activated Sludge ◽  
Sulfate Reduction ◽  
Batch Reactor ◽  
Sulfate Reducing Bacteria ◽  
Phosphate Removal ◽  
Reduction Ratio ◽  
Filamentous Bacteria ◽  
Batch Experiments ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Sulfate reduction in the activated sludge cultivated with glucose and peptone in the sequential batch reactor system and the continuous anaerobic-oxic system was examined. In both systems, sulfate reduction occurred. When the sulfate reduction ratio increased, Type 021N or Beggiatoa sp. grew in the sludge and settling characteristics became poor. These results suggested that sulfate reduction was a cause of growth of the filamentous bacteria which could utilize sulfide for the energy source. There was a tendency that the greater the sulfate reduction ratio was, the smaller the phosphate removal ratio was. As a result of batch experiments using the mixed liquor and the wall growing sludge, it was demonstrated that the sulfate reducing bacteria grown in the activated sludge mainly produced acetate from propionate and lactate. About 107~108 MPN of sulfate reducing bacteria grew in 1 g of the activated sludge and about 109 MPN grew in 1 g of the wall growing sludge.

Interactions between filamentous sulfur bacteria, sulfate reducing bacteria and poly-P accumulating bacteria in anaerobic-oxic activated sludge from a municipal plant

1998 ◽  
Vol 37 (4-5) ◽  
pp. 599-603 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori ◽  
Edja. Kofi. Bosque-Hamilton
Keyword(s):  
Activated Sludge ◽  
Sulfate Reduction ◽  
Reduction Rate ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reduction Rate ◽  
Filamentous Bulking ◽  
Sulfate Reducing ◽  
Sulfur Bacteria ◽  
Sulfate Reduction Rates ◽  
Reducing Bacteria

The interactions between filamentous sulfur bacteria (FSB), sulfate reducing bacteria (SRB) and poly-P accumulating bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions were examined in batch experiments using return sludge (RAS) and settled sewage. Phosphate release and sulfate reduction occurred simultaneously under anaerobic conditions. SRB were more sensitive to temperature changes than PAB. SRB played an important role in the decomposition of propionate to acetate. When the sulfate reduction rates were high, there was a tendency for the maximum release of phosphate also to be high. This was explained by the fact that PAB utilized the acetate produced by SRB. Sulfur oxidizing bacteria were sensitive to temperature change. When the sulfate reduction rate was high, the sulfide oxidizing rate was also high and filamentous bulking occurred. The results showed that sulfate reduction was a cause of filamentous bulking due to Type 021N that could utilize reduced sulfur.

Symbiosis and competition among sulfate reduction, filamentous sulfur, denitrification, and poly-p accumulation bacteria in the anaerobic-oxic activated sludge of a municipal plant

1996 ◽  
Vol 34 (5-6) ◽  
pp. 119-128 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori ◽  
E. J. Bosque-Hamilton
Keyword(s):  
Organic Acids ◽  
Activated Sludge ◽  
Sulfate Reduction ◽  
Sulfide Oxidation ◽  
Sulfate Reducing Bacteria ◽  
Sulfur Cycle ◽  
Sulfate Reducing ◽  
P Accumulation ◽  
Relationship Of ◽  
Reducing Bacteria

Symbiosis and competition were examined among sulfate reducing bacteria (SRB), filamentous sulfur bacteria (FSB), denitrification bacteria (DNB) and poly-P accumulation bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions. Batch experiments were carried out using settled sewage from the same plant as the substrate under several conditions. Under oxic conditions, both sulfate reduction and sulfide oxidation occurred simultaneously, making a symbiotic relationship of SRB and FSB for establishment of a sulfur cycle sustaining the energy requirements. Under anoxic conditions, denitrification was dominant because DNB outcompeted PAB and SRB for organic acids. Under anaerobic conditions, phosphate release and sulfate reduction occurred simultaneously. SRB produced for moles of acetate from four moles of propionate and/or unknown substances by reduction of three moles of sulfate. PAB competed with sulfate-reducing bacteria for organic acids such as propionate. However, PAB utilized acetate produced by SRB.

Control of filamentous bulking and interactions among sulfur oxidation-reduction and iron oxidation-reduction in activated sludge using an iron coagulant

1998 ◽  
Vol 38 (8-9) ◽  
pp. 9-17 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori ◽  
Edja Kofi Bosque-Hamilton
Keyword(s):  
Activated Sludge ◽  
Sulfate Reduction ◽  
Iron Oxidation ◽  
Sulfur Oxidation ◽  
Phosphate Removal ◽  
Aeration Tank ◽  
Filamentous Bulking ◽  
Sulfate Reducing ◽  
Oxidation Reduction ◽  
Reducing Bacteria

The effect of iron coagulant on control of filamentous bulking and phosphate removal was investigated using a laboratory scale activated sludge process. Sulfate reduction was correlated to activated sludge bulking. When FeCl2 was added to the aeration tank, most of the phosphate was removed. Sulfate reduction and filamentous bulking were also suppressed. The addition of FeCl2 was also effective in suppressing phosphate release and sulfide production from wasted sludge. Interactions among sulfur oxidation-reduction and iron oxidation-reduction were examined in the batch experiments. When FeCl2 was added, iron reducing bacteria outcompeted sulfate reducing bacteria and iron oxidizing bacteria grew predominantly.

Microbial ecology of sulfate-reducing bacteria in wastewater biofilms analyzed by microelectrodes and fish (fluorescent in situ hybridization) technique

1999 ◽  
Vol 39 (7) ◽  
pp. 41-47 ◽  
Author(s):  
Satoshi Okabe ◽  
Hisashi Satoh ◽  
Tsukasa Itoh ◽  
Yoshimasa Watanabe
Keyword(s):  
In Situ Hybridization ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Hybridization Technique ◽  
Concentration Profiles ◽  
Reduction Zone ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Culture Dependent

The vertical distribution of sulfate-reducing bacteria (SRB) in microaerophilic wastewater biofilms grown on fully submerged rotating disk reactors (RDR) was determined by the conventional culture-dependent MPN method and in situ hybridization of fluorescently-labelled 16S rRNA-targeted oligonucleotide probes for SRB in parallel. Chemical concentration profiles within the biofilm were also measured using microelectrodes for O2, S2-, NO3- and pH. In situ hybridization revealed that the SRB probe-stained cells were distributed throughout the biofilm even in the oxic surface zone in all states from single scattered cells to clustered cells. The higher fluorescence intensity and abundance of SRB probe-stained cells were found in the middle part of the biofilm. This result corresponded well with O2 and H2S concentration profiles measured by microelectrodes, showing sulfate reduction was restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. Results of the MPN and potential sulfate reducing activity (culture-dependent approaches) indicated a similar distribution of cultivable SRB in the biofilm. The majority of the general SRB probe-stained cells were hybridized with SRB 660 probe, suggesting that one important member of the SRB in the wastewater biofilm could be the genus Desulfobulbus. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from O2 and NO3- respiration zones. Anaerobic H2S oxidation with NO3- was also induced by addition of nitrate to the medium.

scholarly journals Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

2020 ◽  
Vol 9 (6) ◽  
pp. 1920 ◽  
Author(s):  
Ivan Kushkevych ◽  
Jorge Castro Sangrador ◽  
Dani Dordević ◽  
Monika Rozehnalová ◽  
Martin Černý ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Healthy Individuals ◽  
Fecal Samples ◽  
Sulfate Reducing ◽  
Cell Extracts ◽  
Production Of Hydrogen ◽  
Reducing Bacteria

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.

Precipitation of technetium by subsurface sulfate-reducing bacteria

2002 ◽  
Vol 90 (9-11) ◽  
Author(s):  
A. Abdelouas ◽  
Massoud Fattahi ◽  
Bernd Grambow ◽  
L. Vichot ◽  
E. Gautier
Keyword(s):  
Bacterial Growth ◽  
Sulfate Reducing Bacteria ◽  
Deionized Water ◽  
Batch Experiments ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
System Water

SummaryTo study the interaction between Tc and subsurface bacteria, we conducted batch experiments with soil and groundwater or sterilized deionized water. The system water/soil was amended with lactate and phosphate for bacterial growth. Nitrate and sulfate were added to stimulate the growth of indigenous denitrifying and sulfate-reducing bacteria. During denitrification Tc-concentration did not change with time. In the presence of sulfate-reducing bacteria, Tc-concentrations decreased in reacted waters which could be attributed to Tc(VII) reduction and precipitation of TcO

scholarly journals Enhanced sulfate reduction with acidogenic sulfate-reducing bacteria

2008 ◽  
Vol 154 (1-3) ◽  
pp. 1060-1065 ◽  
Author(s):  
Aijie Wang ◽  
Nanqi Ren ◽  
Xu Wang ◽  
Duujong Lee
Keyword(s):  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Reducing Bacteria
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