Evaluation of the wastewater treatment plant of Rome airport

1997 ◽  
Vol 35 (11-12) ◽  
pp. 193-196 ◽  
Author(s):  
R. Gabrieli ◽  
M. Divizia ◽  
D. Donia ◽  
V. Ruscio ◽  
L. Bonadonna ◽  
...  
Keyword(s):  
Treatment Plant ◽  
Enteric Viruses ◽  
Point Of View ◽  
Salmonella Spp ◽  
E Coli ◽  
Three Samples ◽  
Microbiological Parameters ◽  
The Mean ◽  
Somatic Coliphage ◽  
Final Effluent

The wastewater plant of Rome airport, which receives all the sewage from the airport as well as the cess from aeroplanes, was analysed for microbiological parameters. From the bacteriological point of view, in the water and sludge samples the densities of the faecal indicator of pollution and the presence of Salmonella spp and Vibrio cholerae as bacteriological pathogens were determined. At the same time, samples were analysed for the presence of enteric viruses and phages. Overall, the mean reduction of the faecal coliforms was 96%, E. coli 92% and faecal streptococci 99%. Salmonella spp was identified in all but one of the final effluents and V. cholerae in 2/10. Enteric viruses were identified in all but one of the raw waters and in three samples of final effluent. Bacteriophages (somatic coliphage, F-plus phage and B40-8), were found in all the samples but irregularly. Phages and enteric viruses were also found in the prefilter membranes used for prefiltering the raw water samples.

scholarly journals Detection of Enteric Viruses and Bacterial Indicators in a Sewage Treatment Center and Shallow Water Bay

2020 ◽  
Vol 17 (18) ◽  
pp. 6483
Author(s):  
Essam M. Janahi ◽  
Sakina Mustafa ◽  
Saba F. D. Parkar ◽  
Humood A. Naser ◽  
Zaki M. Eisa
Keyword(s):  
Public Health ◽  
Hepatitis A Virus ◽  
Treatment Plant ◽  
Enteric Viruses ◽  
Treated Wastewater ◽  
Treatment Center ◽  
High Concentration ◽  
Salmonella Spp ◽  
Detection Rates ◽  
E Coli

The incidence of enteric viruses in treated wastewater and their potential release into the environment or use for agriculture are very critical matters in public health. In our study, PCR (polymerase chain reaction) analysis of enteric viruses was performed on 59 samples of influents and effluents collected from Tubli wastewater treatment plant (Water Pollution Control Center (WPCC)) and Tubli Bay, where the effluents were discharged, in Kingdom of Bahrain during two sampling periods. Four clinically essential waterborne enteric viruses were examined: enterovirus (EV), hepatitis A virus (HAV), astroviruses (AV), and rotaviruses (RV) and compared to standard bacterial and bacteriophages indicators of fecal pollution. Detection rates of EV, AV, HAV, and RV in the influent samples were 100%, 75%, 12.5%, and 12.5%, respectively, while 50% of the effluent samples from Tubli WPCC contained only EV RNA. None of the tested enteric viruses could be detected in any of the samples collected directly from Tubli Bay. Effluent samples from Tubli plant did not show significant seasonal differences. Since detection of enteric viruses genome does not necessarily indicate infectivity, the infectivity of these viruses was evaluated through isolation and growth of indictor bacteria and bacteriophages. High concentration of fecal bacteriological indicators was detected in all effluents samples (100%): 3.20 × 103 cfu/mL for E. coli, 1.32 × 103 cfu/mL for Salmonella spp., and 1.92 × 103 cfu/mL for Shigella spp. E. coli and Salmonella specific bacteriophages were also detected in the effluent samples in high titers. The combined results of PCR and bacterial enumeration point to a probable public health risk via the use of these wastewaters in agriculture or their discharge into the sea. Continuous surveillance of viral and bacterial prevalence and their resistance to sewage disinfection procedures could contribute to a better control of risks associated with the recycling of effluent wastewater and its release into the environment.

scholarly journals Inspected and non-inspected chilled chicken carcasses commercialized in Viçosa, MG, Brazil: microbiological parameters and Salmonella spp. occurrence

2012 ◽  
Vol 42 (9) ◽  
pp. 1675-1681 ◽  
Author(s):  
Marcus Vinícius Coutinho Cossi ◽  
Michelle Vieira de Almeida ◽  
Mariane Rezende Dias ◽  
Paulo Sérgio de Arruda Pinto ◽  
Luís Augusto Nero
Keyword(s):  
Escherichia Coli ◽  
Food Safety ◽  
Salmonella Spp ◽  
Total Coliforms ◽  
Indicator Microorganisms ◽  
E Coli ◽  
Safety Risks ◽  
Microbiological Parameters ◽  
The Mean

Sixty samples of chilled chicken carcasses submitted (30) and not submitted (30) to Brazilian inspection services were analyzed to investigate if inspected and non-inspected chilled carcasses represented different food safety risks in the region of Viçosa, MG, Brazil. The mean counts of indicator microorganisms (mesophilic aerobes, Enterobacteriaceae, total coliforms and Escherichia coli) of samples belonging to the inspected and non-inspected lots did not present significant differences (P>0.05). Also, no significant differences (P>0.05) were observed for the numbers of Salmonella spp. and E. coli (higher than 2log cfu g-1) between samples submitted or not to inspection. Statistical differences were observed between the two sample classes only for the numbers of mesophilic aerobes higher than 4 and 5log cfu g-1 (P<0.05). The obtained results indicated the limitations of microbiological parameters to differentiate inspected and non-inspected chilled chicken carcasses commercialized in the specific studied area.

scholarly journals Association between animal welfare indicators and microbiological quality of beef carcasses, including Salmonella spp., from a slaughterhouse in Ecuador

2021 ◽  
Vol 14 (4) ◽  
pp. 918-925
Author(s):  
María Cevallos-Almeida ◽  
Ana Burgos-Mayorga ◽  
Carlos A. Gómez ◽  
José Luis Lema-Hurtado ◽  
Leydi Lema ◽  
...  
Keyword(s):  
Animal Welfare ◽  
Microbiological Quality ◽  
Salmonella Spp ◽  
E Coli ◽  
Welfare Indicators ◽  
Beef Carcasses ◽  
Logistic Regressions ◽  
The Mean ◽  
Poor Management

Background and Aim: Pre-slaughter management and slaughter operations are considered critical factors for animal welfare and meat quality. Previous studies have found poor animal welfare management at municipal slaughterhouses in Ecuador, and little is known about how this affects the microbiological quality of the meat. Therefore, the aim of the study was to analyze the association of the microbiological quality of beef carcasses and animal welfare indicators in a municipal slaughterhouse in Ecuador. Materials and Methods: Data for 6 months were collected from a municipal slaughterhouse in Ecuador. Five trained researchers were strategically located along the slaughter process. A total of 351 animals were observed with regard to welfare indicators, and their carcasses were sampled to evaluate microbiological quality. Antemortem (slipping, falling, and vocalization) and postmortem animal welfare indicators (bleed interval, pH, temperature, and bruises) were measured. To determine the total aerobic bacteria (TAB) and Escherichia coli counts and the presence of Salmonella spp., we collected samples by swabbing four different points of each carcass. The association between microbiological quality and animal welfare indicators was studied using univariate and multivariate logistic regressions. Results: The mean TAB count was 5.3 log CFU/cm2, and the mean total count of E. coli was 2.4 log CFU/cm2. Salmonella spp. were isolated in 3.1% of the carcasses. An electric goad was used in all animals, 19.1% slipped at least once, and 19.9% vocalized. The mean pH of the carcasses was 7.2, and 79.2% of carcasses had bruises. Multivariate analysis showed that Salmonella spp. and the TAB count were associated with pH and the number of bruises (p = 0.01 in both cases). Conclusion: Although there was non-significant association between the majority of animal welfare indicators and microbiological quality, the poor management affecting animal welfare and carcass hygiene are worrisome.

Hydraulic behaviour and removal efficiencies of two H-SSF constructed wetlands for wastewater reuse with different operational life

2011 ◽  
Vol 64 (5) ◽  
pp. 1032-1039 ◽  
Author(s):  
S. Barbagallo ◽  
G. L. Cirelli ◽  
A. Marzo ◽  
M. Milani ◽  
A. Toscano
Keyword(s):  
Wastewater Treatment ◽  
Constructed Wetlands ◽  
High Reliability ◽  
Wastewater Reuse ◽  
Treatment Plant ◽  
Breakthrough Curves ◽  
E Coli ◽  
Hydraulic Behaviour ◽  
Operational Life ◽  
The Mean

This work focuses on the performance evaluation of two full-scale horizontal suburface flow constructed wetlands (H-SSF CWs) working in parallel, which have an almost equal surface area (about 2,000 m2) but with different operational lives: 8 and 3 years. Both H-SSF CWs, located in Southern Italy (Sicily), are used for tertiary treatment of the effluent of a conventional wastewater treatment plant. This study evaluates and compares H-SSF CW efficiency both in terms of water quality improvement (removal percentage) and achievement of Italian wastewater discharge and irrigation reuse limits. The mean removal percentage, for the overall operational life, of TSS, COD and BOD (80%, 63%, 58% obtained for H-SSF1 and 67%, 38%, 41% for H-SSF2), confirm the high reliability of CWs for wastewater treatment. However, despite the satisfactory removal of microbial indicators (the mean E. coli removal was up to 2.5 log unit for both beds), CWs didn't achieve the Italian limits for wastewater reuse. Information on hydraulic properties of the CWs were extracted from breakthrough curves of a non-reactive tracer (NaCl). By comparing the nominal (τn) and actual residence time (τ), hydraulic behaviour was revealed.

scholarly journals Prevalence of Escherichia coli and Salmonella spp. in street-vended food of open markets (tianguis) and general hygienic and trading practices in Mexico City

2004 ◽  
Vol 132 (6) ◽  
pp. 1181-1184 ◽  
Author(s):  
T. ESTRADA-GARCIA ◽  
C. LOPEZ-SAUCEDO ◽  
B. ZAMARRIPA-AYALA ◽  
M. R. THOMPSON ◽  
L. GUTIERREZ-COGCO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mexico City ◽  
Phage Type ◽  
Salmonella Spp ◽  
Street Vendors ◽  
E Coli ◽  
Three Samples ◽  
Open Markets ◽  
Average Consumption ◽  
Microbiological Status

Street-vendors in Mexico City provide ready-to-eat food to a high proportion of the inhabitants. Nevertheless, their microbiological status, general hygienic and trading practices are not well known. During spring and summer 2000, five tianguis (open markets) were visited and 48 vendors in 48 stalls interviewed. A total of 103 taco dressings were sampled for E. coli and Salmonella spp.: 44 (43%) contained E. coli and 5 (5%) Salmonella (2 S. Enteritidis phage type 8, 1 S. Agona, 2 S. B group). Both E. coli and salmonellas were isolated from three samples. Of Salmonella-positive stalls 80% (4/5) had three or more food-vendors and 80% of vendors were males, compared with 37·3% (16/43) and 46·4% (20/43) in the Salmonella-negative stalls respectively. Food-vendors kept water in buckets (reusing it all day), lacked toilet facilities, and prepared taco dressings the day before which remained at the tianguis without protection for 7·8 h on average. Consumption of street-vended food by local and tourist populations poses a health risk.

Case study on evaluation of the performance of waste stabilization pond system of Jodhpur, Rajasthan, India

2013 ◽  
Vol 8 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Bhawana Goyal ◽  
Devendra Mohan
Keyword(s):  
Sewage Treatment ◽  
Treatment Plant ◽  
Permissible Limit ◽  
Fecal Coliforms ◽  
E Coli ◽  
Stabilization Pond ◽  
Waste Stabilization Pond ◽  
Waste Stabilization ◽  
Physico Chemical ◽  
Microbiological Parameters

The objective of this study is to find out physico-chemical and microbiological characteristics of influent and effluent generated by the sewage treatment plant of Jodhpur city. The treatment plant was constructed in the year 2003–2004 and based on waste stabilization pond (WSP) system. The treatment capacity of the plant is 45 ML/d. Relationship between different physico-chemical and microbiological parameters were studied. After the treatment of wastewater in WSP, average removal of BOD, COD, TSS and TKN were 84.1, 53.4, 79.7 and 61.7% respectively. Average removal of Total Coliforms (TC), Fecal Coliforms (FC), E. coli and Fecal Streptococci (FS) were 2.79 logs, 2.47 logs, 1.65 logs and 1.58 logs respectively. Maximum efficiency of fecal bacteria removal was found during the summers (2.7 to 4.0 log units) and minimum in winters (1 to 1.8 log unit). Further correlation of temperature, pH and biological oxygen demand with TC, FC, E. coli and FS were studied. Among all these parameters temperature shows highest correlation with microbiological parameters as follows: TC (−0.85), FC (−0.62), E. coli (−0.65) and FS (−0.75). High ambient temperature, long photoperiod and high intensity of light can be considered as the major factor for the removal of fecal indicators during the sewage treatment. After treatment pH, BOD, COD, TSS and TKN were found within the permissible limit of Central Pollution Control Board, India. The observed values of fecal indicator organisms are higher than the permissible limit of World Health Organization (WHO). There are no maturation ponds in the treatment plant; construction of maturation ponds will provide better removal of fecal coliforms.

Effects of different blanching treatments on microbiological profile and quality of the mealworm (Tenebrio molitor)

2019 ◽  
Vol 5 (3) ◽  
pp. 225-234 ◽  
Author(s):  
S. Mancini ◽  
F. Fratini ◽  
T. Tuccinardi ◽  
B. Turchi ◽  
R. Nuvoloni ◽  
...  
Keyword(s):  
Edible Insects ◽  
Salmonella Spp ◽  
E Coli ◽  
Edible Insect ◽  
Microbiological Profile ◽  
Bacterial Endospores ◽  
Microbiological Parameters ◽  
Lower Temperature ◽  
Mealworm Larvae

Safety and quality of edible insects are among the primary aspects which heavily affect edible insect acceptance by the consumers. In this study, the effects of different blanching treatments on the microbiological profile, pH and colour of mealworm larvae were evaluated. The effect of 10 combinations of temperature (50, 60, 70, 80 and 90 °C) and time (2.5 and 5 min) were compared to fresh larvae and oven cooked larvae (10 min at 150 °C). Moreover, the effect of 24 h starvation on the microbiological profile was evaluated. Total viable aerobic count, Enterobacteriaceae, staphylococci, yeasts and moulds, lactic acid bacteria, aerobic bacterial endospores, Escherichia coli, Bacillus cereus, Listeria monocytogenes and Salmonella spp. were determined. Starvation only marginally affected the microflora, furthermore, in all samples E. coli, B. cereus, L. monocytogenes and Salmonella spp. were never detected. A blanching treatment at 60 °C for 5 min seems to be the lower time-temperature combination in order to achieve a significant decrease of microbial loads. Blanching treatments also played a role in pH and colour modifications: larvae blanched at least at 60 °C stopped browning, possibly in relation to an enzymatic inhibition. Among the tested blanching treatments, 60 °C for 5 min seems to be the most feasible application in order to achieve the fixed goals. Lower temperature or time combinations were unable to reduce microbial loads or stop the browning effect, on the other hand, higher temperatures did not allow to improve the product quality and microbiological parameters.

scholarly journals Microbiological Analysis of Tucumã Pulp (Astrocaryum aculeatum Meyer) Commercialized at Fairs in the City of Manaus

2021 ◽  
Vol 10 (9) ◽  
pp. 254-261
Author(s):  
Luana Lopes Casas Rherysonn Pantoja de Jesus ◽  
Lucas Nascimento de Almeida Pedro de Queiroz Costa Neto ◽  
Samaroni Adilson Moreira Corrêa
Keyword(s):  
Escherichia Coli ◽  
Economic Value ◽  
Palm Tree ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
E Coli ◽  
Microbiological Profile ◽  
Three Samples ◽  
Astrocaryum Aculeatum ◽  
The City

The tucumanzeiro is a palm tree of the Arecaceae family, and Astrocaryum aculeatum is the best known and most appreciated species. The fruit called tucumã has a significant cultural and economic value, and despite being widely consumed, the pulp of this fruit has a relatively short shelf life. At fairs, it is possible to see the pulp being stored inappropriately, contributing to the proliferation of microorganisms. Given the importance of this fruit for local trade, this study aimed to investigate the microbiological profile of tucumã pulp sold in the main fairs in Manaus. The pulps were obtained from 18 points of sale divided between the six zones of the city. The samples were placed in a thermal box and submitted to microbiological analysis. The microorganisms evaluated were coliforms (AOAC 991.14), Escherichia coli (AOAC 991.14), Salmonella spp. (ISO 6579), molds and yeasts (APHA 21:2015) in triplicate. The results showed that 13 samples showed coliform growth with amounts ranging from 6.4x10 to 1.2x104 CFU/g. Regarding the amount of yeast, only three samples showed results above what is considered ideal for fruit pulps. Samples that showed growth for molds were within the permissible microbiological limits. None of the samples showed growth for E. coli and Salmonella spp.

Microbiological Status of Australian Sheep Meat

1999 ◽  
Vol 62 (4) ◽  
pp. 380-385 ◽  
Author(s):  
PAUL B. VANDERLINDE ◽  
BARRY SHAY ◽  
JAMES MURRAY
Keyword(s):  
Geometric Mean ◽  
Plate Count ◽  
Salmonella Spp ◽  
Export Markets ◽  
Bacterial Counts ◽  
E Coli ◽  
Coliform Count ◽  
Sheep Meat ◽  
The Mean ◽  
Microbiological Status

Two studies were undertaken to determine the microbiological status of sheep carcass meat and frozen, bulk-packed sheep meat produced in Australia. Samples were collected from 470 sheep carcasses and 415 cartons of frozen sheep trimmings over a period of approximately 12 months. Samples were collected from plants processing sheep carcasses for domestic or export markets. On carcasses, where bacterial counts were obtained, the mean of the log10 aerobic plate count (APC) was 3.92/cm2, the geometric mean of the most probable number (MPN) per square centimeter of Escherichia coli (biotype I) was 23, and the geometric mean of the coliform count was 38 MPN per cm2. A high percentage (75%) of samples was positive for E. coli (biotype I), 81% were positive for coliforms, 5.74% were positive for Salmonella spp., and 1.29% were positive for Campylobacter. Bacterial counts were higher on carcasses chilled over a weekend than on carcasses chilled for 24 h. The total number of bacteria on carcasses processed for domestic markets was similar to that on carcasses processed for export markets. E. coli O157 was not isolated from any of the 465 samples tested. Of the frozen export samples that tested positive, the mean of the log10 APC was 3.47/g, the geometric mean of the E. coli (biotype I) count was 9 MPN per g, and the geometric mean of the coliform count was 19 MPN per g. Of the frozen export samples tested, 48% were positive for E. coli (biotype I), 58% were positive for coliforms, and 6.5% were positive for Salmonella spp. E. coli O157 was recovered from 1 of 343 frozen sheep meat samples tested (0.29%). Bacterial counts were higher on samples of domestic product than on samples of export product. Results from both surveys are compared with data from similar studies conducted in other countries.

scholarly journals Escherichia coli and Salmonella spp. early diagnosis and seasonal monitoring in the sewage treatment process by EMA-qPCR method

2015 ◽  
Vol 64 (2) ◽  
pp. 143-148 ◽  
Author(s):  
MAŁGORZATA KACPRZAK ◽  
KRZYSZTOF FIJAŁKOWSKI ◽  
ANNA GROBELAK ◽  
KAROLINA ROSIKOŃ ◽  
AGNIESZKA RORAT
Keyword(s):  
Escherichia Coli ◽  
Early Diagnosis ◽  
Municipal Wastewater ◽  
Sewage Treatment ◽  
Treatment Plant ◽  
Municipal Wastewater Treatment ◽  
Salmonella Spp ◽  
E Coli ◽  
Four Seasons ◽  
Seasonal Monitoring

Numerous studies have recently shown that molecular biology tools can allow for early diagnosis of pathogens and can substitute existing cost and time-taking traditional methods. One of them, the qPCR, is successfully used in microbiology and its utility has been assessed for many different biological materials. The aim of this study was to: 1) determine, optimize and apply qPCR as a method to detect Escherichia coli and Salmonella spp. in primary influents and final effluents from municipal wastewater treatment plant 2) define if addition of ethidium bromide monoazide (EMA) before DNA extraction can allow to distinguish between alive and dead bacteria, 3) quantify E. coli and Salmonella spp. in wastewater during four seasons by qPCR and traditional spread plate method and determine the correlation between the indicator and pathogenic microorganisms. The obtained results has shown that qPCR can be used as a quantitative method in the diagnosis of investigated bacteria in wastewater with EMA pretreatment as a crucial step for a proper quantitative analysis of the presence of these bacteria in wastewater. Both E. coli and Salmonella spp. bacteria species were present in all samples of primary influents and final effluents. Our study shown that the quantity of investigated bacteria is strictly correlated with the season that they were obtained in.

Sign in / Sign up

Export Citation Format

Share Document