A novel salt-tolerant mutant YWL-01 for the treatment of saline wastewater

2009 ◽  
Vol 60 (11) ◽  
pp. 2869-2877 ◽  
Author(s):  
J. Liu ◽  
G. L. Wang
Keyword(s):  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Sewage Treatment ◽  
Oxygen Demand ◽  
Salt Tolerant ◽  
Bacterial Strains ◽  
Saline Wastewater ◽  
Tolerant Mutant ◽  
Combined Mutagenesis ◽  
Salt Tolerant Mutant

The treatment of high-saline wastewater from some salt-end markets including agro-food industry is a serious problem yet to be solved in some coastal cities. The conventional physical–chemical techniques are energy-consuming and their startup and running costs are still high. Biological methods using salt-tolerant bacterial strains for the treatment of hypersaline wastewater provide one possible solution. In this study, one salt-tolerant mutant named YWL-01 was screened out by sewage treatment and proved to be a genetically stable salt-tolerant strain for saline wastewater treatment. First, combined mutagenesis was done on an isolated sewage treatment strain Bacillus Y for the screening of salt tolerance, and 11 mutants were obtained after subculture for many times. Then, a secondary screening test was performed for COD (chemical oxygen demand) and TOC (total organic carbon) removal efficiency analyses. At last, the best mutant YWL-01 with increased capacity to treat saline wastewater was chosen for use. RAPD (Random Amplified Polymorphic DNA) analysis of genetic stability on the mutant YWL-01 showed that it is a hereditary mutant for the treatment of high-saline wastewater.

scholarly journals Isolation of two salt-tolerant strains from activated sludge and its COD degradation characteristics from saline organic wastewater

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Guizhong Zhou ◽  
Xitong Wang ◽  
Huiyang Zhao ◽  
Weiqian Zhang ◽  
Guishan Liu ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Biological Treatment ◽  
Soluble Sugar ◽  
Oxygen Demand ◽  
Salt Tolerant ◽  
Bacterial Strains ◽  
Saline Wastewater ◽  
High Salt Stress ◽  
Relative Membrane Permeability ◽  
Organic Wastewater

Abstract The efficient biological treatment of saline wastewater has been limited by the low activities of microorganisms under saline conditions. High salinity poses unbalance osmotic stress across the cell wall and even leads to cell plasmolysis. In this work, we aim to isolate salt-tolerant bacterial strains from activated sludge, and apply them for degrading chemical oxygen demand (COD) of saline organic wastewater. Two salt-tolerant strains were screened and isolated from activated sludge, which was domesticated with salty water for over 300 days. The two strains were identified as Bacillus cereus (strain A) and Bacillus anthracis (strain B) through 16S rRNA sequencing. The degradation characteristics of strain A were explored. The results showed the relative membrane permeability of strain A remained stable under high salt stress, which glycine and proline play an important role to maintain cell osmotic. The protein and soluble sugar amounts of strain were increased by higher salt concentrations. In simulating saline wastewater, the optimum culture temperature, pH, salinity, influent COD concentration and inoculation amount of strain A were 35 °C, 9, 4%, 8000 mg L−1, 6%, respectively. Optimal conditions could provide guidance for the treatment of practical saline wastewater. The linear regression model of each impact factor built based on the result PB experiment revealed that cross-linking time has the most significant influence on COD removal for salt-tolerant strains. It will provide theoretical basis for biological treatment of saline organic wastewater.

scholarly journals Physiological Basis and Transcriptional Profiling of Three Salt-Tolerant Mutant Lines of Rice

2016 ◽  
Vol 7 ◽  
Author(s):  
Concha Domingo ◽  
Eric Lalanne ◽  
María M. Catalá ◽  
Eva Pla ◽  
Juan L. Reig-Valiente ◽  
...  
Keyword(s):  
Transcriptional Profiling ◽  
Salt Tolerant ◽  
Physiological Basis ◽  
Tolerant Mutant ◽  
Mutant Lines ◽  
Salt Tolerant Mutant

Ternary cycle treatment of high saline wastewater from pesticide production using a salt-tolerant microorganism

2013 ◽  
Vol 67 (9) ◽  
pp. 1960-1966 ◽  
Author(s):  
Xiang Wu ◽  
Ya-guang Du ◽  
Yi Qu ◽  
Dong-yun Du
Keyword(s):  
Gene Sequencing ◽  
Oxygen Demand ◽  
Ethyl Chloride ◽  
Aerobic Treatment ◽  
Salt Tolerant ◽  
Chemical Oxygen Demand Removal ◽  
Saline Wastewater ◽  
Nacl Concentration ◽  
Ph Range ◽  
Immobilized Microorganism

The material of this study is provided by biological aerobic treatment of high saline wastewater from pesticide production. The microorganism used for biodegradation has been identified by gene-sequencing as a strain of Bacillus sp. SCUN. The best growth condition for the salt-tolerant microorganism has been studied by varying the pH, immobilized microorganism dosage and temperature conditions. The feasibility of pretreating wastewater in ethyl chloride production containing 4% NaCl has been discussed. It was found that under the pH range of 6.0–8.0, immobilized microorganism dosage of 1.5 g/L, temperature of 30 °C, and NaCl concentration of 0–3%, the microorganism achieves the best growth for biodegradation. After domestication, the strain can grow under 4% NaCl. This salt-tolerant microorganism is effective in the pretreated high saline wastewater. With a newly developed ternary cycle treatment, the chemical oxygen demand removal approaches 58.3%. The theoretical basis and a new method for biological treatments in biodegradation of high saline wastewater in ethyl chloride production are discussed.

Screening for high productive salt tolerant mutant M4 lines in chickpea(Cicer arietinum L.)

2017 ◽  
Author(s):  
Mousumi Das ◽  
Saby Asachi Kundagrami
Keyword(s):  
Cicer Arietinum ◽  
Seed Yield ◽  
Gamma Rays ◽  
Genetic Improvement ◽  
Yield Potential ◽  
Salt Tolerant ◽  
Cicer Arietinum L ◽  
Tolerant Mutant ◽  
Salt Tolerant Mutant ◽  
Stressed Field

Genetic improvement through induced mutation has been very effective in improvement of crops. Seeds of three popular chickpea variety namely BGM 408, B115 and JG 315 were treated with 10,20 and 30 kR of gamma rays. Then treated seeds with their respective control(0 kR gamma rays) were sown in the field of Calcutta University experimental farm,Baruipur (with no salinity) in three consecutive years to raise M1, M2 and M3 generations respectively.Some M3 lines having higher yield potential were subjected to grow as M4 lines in salt stressed field (5-7ds m-1) of Hingalganj,South 24 Parganas. Finally, some promising salt tolerant lines with improved seed yield were selected which have a good promise for coastal saline belt after releasing as a variety.

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